{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:54:32Z","timestamp":1790891672394,"version":"4.1.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.talanta.2026.130382","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T23:24:28Z","timestamp":1785281068000},"page":"130382","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"PA","title":["Integrated proteomic and functional mass spectrometry workflow for comprehensive ricin characterization at trace levels"],"prefix":"10.1016","volume":"312","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1683-3925","authenticated-orcid":false,"given":"Ylane","family":"Idir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Piquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kodjo","family":"Nouwade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Dano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4655-1695","authenticated-orcid":false,"given":"Maria Lucia","family":"Orsini Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Thuret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6787-4149","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Fenaille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1075-9980","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Becher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2026.130382_bib1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.2165\/00139709-200322010-00007","article-title":"Ricin poisoning","volume":"22","author":"Bradberry","year":"2003","journal-title":"Toxicol. Rev."},{"key":"10.1016\/j.talanta.2026.130382_bib2","doi-asserted-by":"crossref","first-page":"8128","DOI":"10.1016\/S0021-9258(18)47538-2","article-title":"RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes","volume":"262","author":"Endo","year":"1987","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib3","doi-asserted-by":"crossref","first-page":"4906","DOI":"10.3390\/toxins7124856","article-title":"Characterization of Ricin and R. communis agglutinin reference materials","volume":"7","author":"Worbs","year":"2015","journal-title":"Toxins"},{"key":"10.1016\/j.talanta.2026.130382_bib4","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.bios.2007.11.005","article-title":"A hand-held surface plasmon resonance biosensor for the detection of ricin and other biological agents","volume":"23","author":"Feltis","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2026.130382_bib5","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1021\/ac802769s","article-title":"Mass spectrometric detection of ricin and its activity in food and clinical samples","volume":"81","author":"Kalb","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib6","doi-asserted-by":"crossref","first-page":"4154","DOI":"10.1021\/pr8003437","article-title":"Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry","volume":"7","author":"Duriez","year":"2008","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2026.130382_bib7","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1021\/ac061498b","article-title":"Detection of functional ricin by immunoaffinity and liquid Chromatography\u2212Tandem Mass spectrometry","volume":"79","author":"Becher","year":"2007","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib8","first-page":"7940","article-title":"Simultaneous analysis of depurinated nucleic acid stem-loop and free adenine for ricin toxicity assay by hydrophilic interaction liquid chromatography-high-resolution mass spectrometry (HILIC-HRMS)","volume":"16","author":"Miyaguchi","year":"2024","journal-title":"Anal. Methods Adv. Methods Appl."},{"key":"10.1016\/j.talanta.2026.130382_bib9","doi-asserted-by":"crossref","first-page":"177","DOI":"10.3390\/toxins17040177","article-title":"Establishment and comparison of detection methods for ricin and Abrin based on their depurination activities","volume":"17","author":"Dong","year":"2025","journal-title":"Toxins"},{"key":"10.1016\/j.talanta.2026.130382_bib10","doi-asserted-by":"crossref","first-page":"6867","DOI":"10.1021\/acs.analchem.6b01486","article-title":"Improved sensitivity for the qualitative and quantitative analysis of active ricin by MALDI-TOF mass spectrometry","volume":"88","author":"Wang","year":"2016","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib11","doi-asserted-by":"crossref","DOI":"10.1021\/acs.chemrestox.4c00149","article-title":"Mass spectrometric detection and differentiation of enzymatically active Abrin and ricin combined with a novel affinity enrichment technique","author":"Drinkard","year":"2024","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.talanta.2026.130382_bib12","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.toxicon.2015.01.003","article-title":"Quantification of ricin, RCA and comparison of enzymatic activity in 18 Ricinus communis cultivars by isotope dilution mass spectrometry, Toxicon Off","volume":"95","author":"Schieltz","year":"2015","journal-title":"J. Int. Soc. Toxinology"},{"key":"10.1016\/j.talanta.2026.130382_bib13","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.bios.2015.11.020","article-title":"Simultaneous differentiation and quantification of ricin and agglutinin by an antibody-sandwich surface plasmon resonance sensor","volume":"78","author":"Stern","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2026.130382_bib14","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1007\/s00216-020-03030-8","article-title":"Quantitative detection of ricin in beverages using trypsin\/Glu-C tandem digestion coupled with ultra-high-pressure liquid chromatography-tandem mass spectrometry","volume":"413","author":"Liang","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib15","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/pmic.202400338","article-title":"Top-down proteomics: why and when?","volume":"25","author":"Kaulich","year":"2025","journal-title":"Proteomics"},{"key":"10.1016\/j.talanta.2026.130382_bib16","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1021\/acs.jproteome.0c00458","article-title":"Direct acetonitrile-assisted trypsin digestion method combined with LC-MS\/MS-Targeted peptide analysis for unambiguous identification of intact ricin","volume":"20","author":"Liu","year":"2021","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2026.130382_bib18","first-page":"182","article-title":"The identification of disulfides in ricin D using proteolytic cleavage followed by negative-ion nano-electrospray ionization mass spectrometry of the peptide fragments, Rapid commun","volume":"29","author":"Tran","year":"2015","journal-title":"Mass Spectrom. RCM"},{"key":"10.1016\/j.talanta.2026.130382_bib19","doi-asserted-by":"crossref","first-page":"243","DOI":"10.3390\/toxins16060243","article-title":"Glycan profile and sequence variants of certified Ricin reference material and other Ricin samples yield unique molecular signature features","volume":"16","author":"Josuran","year":"2024","journal-title":"Toxins"},{"key":"10.1016\/j.talanta.2026.130382_bib20","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/mas.21540","article-title":"The Skyline ecosystem: informatics for quantitative mass spectrometry proteomics","volume":"39","author":"Pino","year":"2020","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.talanta.2026.130382_bib21","doi-asserted-by":"crossref","first-page":"4881","DOI":"10.3390\/toxins7124854","article-title":"Recommended mass spectrometry-based strategies to identify ricin-containing samples","volume":"7","author":"Kalb","year":"2015","journal-title":"Toxins"},{"key":"10.1016\/j.talanta.2026.130382_bib22","doi-asserted-by":"crossref","first-page":"5145","DOI":"10.1007\/s00216-024-05452-0","article-title":"Rapid detection of ricin at trace levels in complex matrices by asialofetuin-coated beads and bottom-up proteomics using high-resolution mass spectrometry","volume":"416","author":"Piquet","year":"2024","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib23","doi-asserted-by":"crossref","first-page":"8473","DOI":"10.1021\/acs.analchem.5b01900","article-title":"Multiplex quantification of protein toxins in human biofluids and food matrices using immunoextraction and high-resolution targeted mass spectrometry","volume":"87","author":"Dupr\u00e9","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib24","doi-asserted-by":"crossref","first-page":"412","DOI":"10.3390\/toxins16100412","article-title":"A monoclonal antibody with a high affinity for ricin isoforms D and E provides strong protection against ricin poisoning","volume":"16","author":"Lequesne","year":"2024","journal-title":"Toxins"},{"key":"10.1016\/j.talanta.2026.130382_bib25","doi-asserted-by":"crossref","DOI":"10.1080\/19420862.2025.2544922","article-title":"Combining deep mutational scanning and SPR binning approaches for large-scale epitope identification of anti-ricin antibodies","volume":"17","author":"Kot","year":"2025","journal-title":"mAbs"},{"key":"10.1016\/j.talanta.2026.130382_bib26","doi-asserted-by":"crossref","first-page":"4399","DOI":"10.1007\/s00204-025-04132-x","article-title":"Polyclonal antibodies towards abrin and ricin-design and potential application for mass spectrometry-based analysis of human biosamples","volume":"99","author":"Vollmer","year":"2025","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.talanta.2026.130382_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2023.464373","volume":"1708","author":"Liang","year":"2023","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2026.130382_bib28","doi-asserted-by":"crossref","first-page":"5147","DOI":"10.1007\/s00216-014-7710-2","article-title":"Identification and quantification of ricin in biomedical samples by magnetic immunocapture enrichment and liquid chromatography electrospray ionization tandem mass spectrometry","volume":"406","author":"Ma","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2025.115942","article-title":"Mass spectrometric identification of novel truncated \u03b1-synuclein species following optimized immunoprecipitation from human brain tissue","volume":"706","author":"Van","year":"2025","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.talanta.2026.130382_bib30","doi-asserted-by":"crossref","first-page":"3068","DOI":"10.1021\/acs.jproteome.7b00432","article-title":"Quantitative assessment of the effects of trypsin digestion methods on affinity purification\u2013mass spectrometry-based protein\u2013protein interaction analysis","volume":"16","author":"Zhang","year":"2017","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2026.130382_bib31","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jchromb.2017.03.044","article-title":"To elute or not to elute in immunocapture bottom-up LC\u2013MS","volume":"1055\u20131056","author":"Levern\u00e6s","year":"2017","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.talanta.2026.130382_bib32","doi-asserted-by":"crossref","first-page":"6425","DOI":"10.1007\/s00216-021-03608-w","article-title":"Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-\u03b1","volume":"413","author":"Laborie","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib33","doi-asserted-by":"crossref","first-page":"4988","DOI":"10.1021\/acs.jproteome.5c00327","article-title":"High-performance proteomics using Nano-, Capillary-, and microflow chromatographic separations","volume":"24","author":"Tsiklauri","year":"2025","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2026.130382_bib34","doi-asserted-by":"crossref","first-page":"12339","DOI":"10.1021\/acs.analchem.3c01772","article-title":"Easy, robust, and repeatable online acid cleavage of proteins in Mobile phase for fast quantitative LC-MS Bottom-Up protein analysis\u2500application for Ricin detection","volume":"95","author":"Naplekov","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib35","doi-asserted-by":"crossref","first-page":"3750","DOI":"10.1038\/s41596-024-01033-8","article-title":"Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP","volume":"19","author":"Leduc","year":"2024","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.talanta.2026.130382_bib36","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/s41592-024-02559-1","article-title":"Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity","volume":"22","author":"Bubis","year":"2025","journal-title":"Nat. Methods"},{"key":"10.1016\/j.talanta.2026.130382_bib37","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1021\/ac102571f","article-title":"Detection and quantification of ricin in beverages using isotope dilution tandem mass spectrometry","volume":"83","author":"McGrath","year":"2011","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130382_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbi.2022.110055","article-title":"Inactivation of ricin by constituents present in a skin decontamination lotion","volume":"365","author":"Van Den Berg","year":"2022","journal-title":"Chem. Biol. Interact."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914026010386?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914026010386?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:59:25Z","timestamp":1790888365000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914026010386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":37,"alternative-id":["S0039914026010386"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2026.130382","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated proteomic and functional mass spectrometry workflow for comprehensive ricin characterization at trace levels","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2026.130382","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"130382"}}