{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:32:49Z","timestamp":1793140369702,"version":"4.5.0"},"reference-count":147,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordination for the improvement of Higher Education Personnel","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["465571\/2014-0"],"award-info":[{"award-number":["465571\/2014-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","award":["2014\/50945-4"],"award-info":[{"award-number":["2014\/50945-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.talanta.2026.130509","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:54:45Z","timestamp":1787673285000},"page":"130509","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"PB","title":["Analytical advances in citrus disease detection: Conventional molecular assays and emerging analytical technologies"],"prefix":"10.1016","volume":"312","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4911-3295","authenticated-orcid":false,"given":"Lucas Moreira","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Aparecido Pupim","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideko","family":"Yamanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2026.130509_bib1","series-title":"Recent Insights on Citrus Diversity and Phylogeny","author":"Luro","year":"2017"},{"key":"10.1016\/j.talanta.2026.130509_bib3","series-title":"The Orange Juice Landscape","author":"Neves","year":"2011"},{"key":"10.1016\/j.talanta.2026.130509_bib4","doi-asserted-by":"crossref","first-page":"164","DOI":"10.33545\/26174693.2025.v9.i3Sc.3938","article-title":"The complex world of citrus tristeza virus: a review on its impact on citrus production and management approaches","volume":"9","author":"Kalita","year":"2025","journal-title":"International Journal of Advanced Biochemistry Research"},{"key":"10.1016\/j.talanta.2026.130509_bib6","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1094\/PHYTO-11-16-0419-R","article-title":"Sexual reproduction in the citrus black spot pathogen, Phyllosticta citricarpa","volume":"107","author":"Tran","year":"2017","journal-title":"Phytopathology"},{"key":"10.1016\/j.talanta.2026.130509_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5070\/C421028302","article-title":"Where have all the flowers gone? Postbloom fruit drop of citrus in the americas","volume":"2","author":"Timmer","year":"2015","journal-title":"J. Citrus Pathol."},{"key":"10.1016\/j.talanta.2026.130509_bib8","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/ppa.12930","article-title":"Spatial patterns of the citrus leprosis virus and its associated mite vector in systems without intervention","volume":"68","author":"Bassanezi","year":"2019","journal-title":"Plant Pathol."},{"key":"10.1016\/j.talanta.2026.130509_bib9","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.3390\/v12121353","article-title":"Walking together: cross-protection, genome conservation, and the replication machinery of citrus tristeza virus","volume":"12","author":"Folimonova","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.talanta.2026.130509_bib10","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.3390\/agronomy13041112","article-title":"Citrus canker: a persistent threat to the worldwide citrus industry\u2014an analysis","volume":"13","author":"Ali","year":"2023","journal-title":"Agronomy"},{"key":"10.1016\/j.talanta.2026.130509_bib11","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1094\/MPMI-09-13-0292-R","article-title":"The complete genome sequence of \u2018 candidatus liberibacter americanus\u2019, associated with citrus huanglongbing","volume":"27","author":"Wulff","year":"2014","journal-title":"MPMI (Mol. Plant-Microbe Interact.)"},{"key":"10.1016\/j.talanta.2026.130509_bib12","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1128\/aem.59.4.1143-1148.1993","article-title":"Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method","volume":"59","author":"Hartung","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.talanta.2026.130509_bib13","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1023\/A:1024219629669","article-title":"Development and validation of a fast PCR-based detection method for pathogenic isolates of the citrus black spot fungus, Guignardia citricarpa","volume":"109","author":"Bonants","year":"2003","journal-title":"Eur. J. Plant Pathol."},{"key":"10.1016\/j.talanta.2026.130509_bib14","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jviromet.2017.02.012","article-title":"Production of mono- and polyclonal antibodies to citrus leprosis virus C2 and their application in triple antibody sandwich ELISA and immunocapture RT-PCR diagnostic assays","volume":"243","author":"Choudhary","year":"2017","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.talanta.2026.130509_bib15","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.3390\/microorganisms12081539","article-title":"Serological and molecular detection of citrus tristeza virus: a review","volume":"12","author":"Shang","year":"2024","journal-title":"Microorganisms"},{"key":"10.1016\/j.talanta.2026.130509_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2019.07.005","article-title":"Ultrasensitive immunoassay for detection of citrus tristeza virus in citrus sample using disposable microfluidic electrochemical device","volume":"205","author":"Freitas","year":"2019","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2026.130509_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2024.127132","article-title":"Impedimetric immunosensor versus qPCR for huanglongbing detection","volume":"283","author":"Silva","year":"2025","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2026.130509_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.electacta.2025.147893","article-title":"Electrochemical impedance spectroscopy as a tool to investigate the electrochemical interface in detection of huanglongbing bacterium using a magnetic nanoparticle-based immunosensor","volume":"548","author":"Silva","year":"2026","journal-title":"Electrochim. Acta"},{"key":"10.1016\/j.talanta.2026.130509_bib19","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.aca.2018.09.031","article-title":"Electrochemical detection of plant virus using gold nanoparticle-modified electrodes","volume":"1046","author":"Khater","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2026.130509_bib20","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s40858-025-00766-5","article-title":"Development of a novel duplex PCR-Lateral flow immunoassay (d-PCR-LFIA) for simultaneous detection of candidatus Liberibacter asiaticus and citrus tristeza virus","volume":"50","author":"Bharsakale","year":"2025","journal-title":"Trop. Plant Pathol."},{"key":"10.1016\/j.talanta.2026.130509_bib21","doi-asserted-by":"crossref","first-page":"5474","DOI":"10.3390\/su16135474","article-title":"Raman spectroscopy for plant disease detection in next-generation agriculture","volume":"16","author":"Saletnik","year":"2024","journal-title":"Sustainability"},{"key":"10.1016\/j.talanta.2026.130509_bib22","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1002\/jrs.5741","article-title":"Detection and identification of canker and blight on orange trees using a hand\u2010held raman spectrometer","volume":"50","author":"Sanchez","year":"2019","journal-title":"J. Raman Spectrosc."},{"key":"10.1016\/j.talanta.2026.130509_bib23","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1021\/acs.jafc.0c07205","article-title":"Advanced application of raman spectroscopy and surface-enhanced raman spectroscopy in plant disease diagnostics: a review","volume":"69","author":"Weng","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.talanta.2026.130509_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.cropro.2024.107003","article-title":"Early plant disease detection by raman spectroscopy: an open-source software designed for the automation of preprocessing and analysis of spectral dataset","volume":"188","author":"Vallejo P\u00e9rez","year":"2025","journal-title":"Crop Prot."},{"key":"10.1016\/j.talanta.2026.130509_bib25","doi-asserted-by":"crossref","first-page":"24203","DOI":"10.1021\/acsomega.4c02104","article-title":"Application and research progress of laser-induced breakdown spectroscopy in agricultural product inspection","volume":"9","author":"Wang","year":"2024","journal-title":"ACS Omega"},{"key":"10.1016\/j.talanta.2026.130509_bib26","doi-asserted-by":"crossref","first-page":"386","DOI":"10.3390\/foods14030386","article-title":"Advances in computer vision and spectroscopy techniques for non-destructive quality assessment of citrus fruits: a comprehensive review","volume":"14","author":"Yu","year":"2025","journal-title":"Foods"},{"key":"10.1016\/j.talanta.2026.130509_bib27","article-title":"Recent advances in agricultural disease image recognition technologies: a review, concurr","volume":"35","author":"Bondre","year":"2023","journal-title":"Comput. Times"},{"key":"10.1016\/j.talanta.2026.130509_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.compag.2022.106808","article-title":"Combining multicolor fluorescence imaging with multispectral reflectance imaging for rapid citrus huanglongbing detection based on lightweight convolutional neural network using a handheld device","volume":"194","author":"He","year":"2022","journal-title":"Comput. Electron. Agric."},{"key":"10.1016\/j.talanta.2026.130509_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.apr.2022.101325","article-title":"Machine learning method using camera image patterns for predictions of particulate matter concentrations","volume":"13","author":"Suh","year":"2022","journal-title":"Atmos. Pollut. Res."},{"key":"10.1016\/j.talanta.2026.130509_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2024.106688","article-title":"Study on the citrus greening disease: current challenges and novel therapies","volume":"192","author":"Limayem","year":"2024","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.talanta.2026.130509_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.pmpp.2023.102016","article-title":"Citrus Huanglongbing (HLB): Diagnostic and management options","volume":"125","author":"Thakuria","year":"2023","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"10.1016\/j.talanta.2026.130509_bib32","article-title":"Optical screening of citrus leaf diseases using label-free spectroscopic tools: a review","volume":"18","author":"Bharadwaj","year":"2024","journal-title":"J. Agric. Food Res."},{"key":"10.1016\/j.talanta.2026.130509_bib33","doi-asserted-by":"crossref","first-page":"500","DOI":"10.3390\/computers14110500","article-title":"Deep learning-based citrus canker and huanglongbing disease detection using leaf images","volume":"14","author":"Devora-Guadarrama","year":"2025","journal-title":"Computers"},{"key":"10.1016\/j.talanta.2026.130509_bib34","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1007\/s11192-017-2300-7","article-title":"Citation-based clustering of publications using CitNetExplorer and VOSviewer","volume":"111","author":"van Eck","year":"2017","journal-title":"Scientometrics"},{"key":"10.1016\/j.talanta.2026.130509_bib35","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1094\/PDIS-04-17-0537-RE","article-title":"Critical fungicide spray period for citrus black spot control in S\u00e3o Paulo State, Brazil","volume":"102","author":"Lanza","year":"2018","journal-title":"Plant Dis."},{"key":"10.1016\/j.talanta.2026.130509_bib36","doi-asserted-by":"crossref","DOI":"10.1590\/0100-29452019422","article-title":"Advances in citrus propagation in Brazil","volume":"41","author":"de Carvalho","year":"2019","journal-title":"Rev. Bras. Frutic."},{"key":"10.1016\/j.talanta.2026.130509_bib37","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.cropro.2007.04.007","article-title":"Evaluation of fungicide applications to sweet orange at different flowering stages for control of postbloom fruit drop caused by Colletotrichum acutatum","volume":"27","author":"de Goes","year":"2008","journal-title":"Crop Prot."},{"key":"10.1016\/j.talanta.2026.130509_bib38","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.cropro.2014.01.012","article-title":"Efficacy and timing of application of fungicides for control of citrus postbloom fruit drop","volume":"59","author":"Silva-Junior","year":"2014","journal-title":"Crop Prot."},{"key":"10.1016\/j.talanta.2026.130509_bib39","series-title":"Pesticides Classification and its Impact on Human and Environment","first-page":"140","author":"Yadav","year":"2017"},{"key":"10.1016\/j.talanta.2026.130509_bib40","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/B:APPA.0000006546.55305.e3","article-title":"Brevipalpus-transmitted plant virus and virus-like diseases: cytopathology and some recent cases","volume":"30","author":"Kitajima","year":"2003","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.talanta.2026.130509_bib41","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1099\/vir.0.82038-0","article-title":"Complete nucleotide sequence, genomic organization and phylogenetic analysis of citrus leprosis virus cytoplasmic type","volume":"87","author":"Locali-Fabris","year":"2006","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.talanta.2026.130509_bib42","series-title":"Citros, Instituto Agron\u00f4mico E Fundag","article-title":"Doen\u00e7as de citros causadas por v\u00edrus e vir\u00f3ides","author":"A","year":"2005"},{"key":"10.1016\/j.talanta.2026.130509_bib43","doi-asserted-by":"crossref","first-page":"78","DOI":"10.5958\/2249-524X.2021.00023.6","article-title":"Combined effects of azadirachtin and citrus limonum essential oil against sitophilus granarius: toxicity and biological activities","volume":"33","author":"Guettal","year":"2021","journal-title":"Pestic. Res. J."},{"key":"10.1016\/j.talanta.2026.130509_bib44","article-title":"Lethal and sublethal effects of pesticides used in Brazilian citrus groves on Panonychus citri (Acari: tetranychidae)","volume":"85","author":"Alves","year":"2018","journal-title":"Arq. Inst. Biol. (Sao. Paulo)."},{"key":"10.1016\/j.talanta.2026.130509_bib45","series-title":"Plant Health Instructor","article-title":"Cancro c\u00edtrico","author":"Gottwald","year":"2013"},{"key":"10.1016\/j.talanta.2026.130509_bib46","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1094\/PHP-2002-0812-01-RV","article-title":"Citrus canker: the pathogen and its impact","volume":"3","author":"Gottwald","year":"2002","journal-title":"Plant Health Prog."},{"key":"10.1016\/j.talanta.2026.130509_bib47","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1094\/MPMI-22-8-1011","article-title":"Complete genome sequence of citrus huanglongbing bacterium, \u2018 Candidatus Liberibacter asiaticus\u2019 obtained through metagenomics","volume":"22","author":"Duan","year":"2009","journal-title":"MPMI (Mol. Plant-Microbe Interact.)"},{"key":"10.1016\/j.talanta.2026.130509_bib48","article-title":"Manejo do greening: 10 mandamentos para o sucesso no controle da doen\u00e7a","volume":"1","author":"Ayres","year":"2019","journal-title":"Fundo de Defesa da Citricultura, Araraquara"},{"key":"10.1016\/j.talanta.2026.130509_bib49","series-title":"USE OF MOLECULAR AND BIOCHEMICAL METHODS TO DETERMINE CITRUS TRISTEZA VIRUS (CTV) VIRAL COMPONENTS AND RESISTANCE IN CANDIDATE ROOTSTOCKS TO REPLACE SOUR ORANGE","author":"Hosni","year":"2009"},{"key":"10.1016\/j.talanta.2026.130509_bib50","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/epp.12908","article-title":"Citrus tristeza virus","volume":"53","year":"2023","journal-title":"EPPO Bull."},{"key":"10.1016\/j.talanta.2026.130509_bib51","series-title":"MANUAL DE PINTA PRETA MEDIDAS ESSENCIAIS DE CONTROLE","first-page":"1","year":"2018"},{"key":"10.1016\/j.talanta.2026.130509_bib52","series-title":"MANUAL DE PODRID\u00c3O FLORAL MEDIDAS ESSENCIAIS DE CONTROLE PODRID\u00c3O FLORAL","first-page":"13","year":"2018"},{"key":"10.1016\/j.talanta.2026.130509_bib53","series-title":"FUNDECITRUS, HLB\/Greening","year":"2022"},{"key":"10.1016\/j.talanta.2026.130509_bib56","series-title":"FUNDECITRUS, Leprose","year":"2022"},{"key":"10.1016\/j.talanta.2026.130509_bib57","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0168-1702(00)00191-X","article-title":"Progress on strain differentiation of Citrus tristeza virus and its application to the epidemiology of citrus tristeza disease","volume":"71","author":"Niblett","year":"2000","journal-title":"Virus Res."},{"key":"10.1016\/j.talanta.2026.130509_bib58","first-page":"97","article-title":"Pseudomonas citri, the cause of citrus canker","volume":"4","author":"Hasse","year":"1915","journal-title":"J. Agric. Res."},{"key":"10.1016\/j.talanta.2026.130509_bib59","doi-asserted-by":"crossref","DOI":"10.1146\/annurev-virology-100520-114412","article-title":"Citrus tristeza virus : from pathogen to Panacea","volume":"9","author":"Folimonova","year":"2022","journal-title":"Annu. Rev. Virol."},{"key":"10.1016\/j.talanta.2026.130509_bib60","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/jipb.12437","article-title":"Huanglongbing: an overview of a complex pathosystem ravaging the world's citrus","volume":"58","author":"da Gra\u00e7a","year":"2016","journal-title":"J. Integr. Plant Biol."},{"key":"10.1016\/j.talanta.2026.130509_bib61","doi-asserted-by":"crossref","unstructured":"J.M.S. Bartlett, D. Stirling, A short history of the polymerase chain reaction, in: PCR Protocols, Humana Press, New Jersey, n.d.: pp. 3\u20136. https:\/\/doi.org\/10.1385\/1-59259-384-4:3.","DOI":"10.1385\/1-59259-384-4:3"},{"key":"10.1016\/j.talanta.2026.130509_bib62","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/BF00918012","article-title":"Polymerase chain reaction","volume":"9","author":"Erlich","year":"1989","journal-title":"J. Clin. Immunol."},{"key":"10.1016\/j.talanta.2026.130509_bib63","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1128\/AEM.67.6.2849-2852.2001","article-title":"Quantitative PCR method for diagnosis of citrus bacterial canker","volume":"67","author":"Cubero","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.talanta.2026.130509_bib64","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1006\/mcpr.1996.0006","article-title":"PCR detection of the two 'Candidatus\" liberobacter species associated with greening disease of citrus","volume":"10","author":"Jagoueix","year":"1996","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.talanta.2026.130509_bib65","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1094\/PDIS.2003.87.11.1317","article-title":"Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the americas","volume":"87","author":"Locali","year":"2003","journal-title":"Plant Dis."},{"key":"10.1016\/j.talanta.2026.130509_bib66","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/095530000138952","article-title":"Effect of ethidium bromide intercalation on DNA radiosensitivity","volume":"76","author":"Begusova","year":"2000","journal-title":"Int. J. Radiat. Biol."},{"key":"10.1016\/j.talanta.2026.130509_bib67","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1128\/jb.127.3.1550-1557.1976","article-title":"Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus","volume":"127","author":"Chien","year":"1976","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.talanta.2026.130509_bib68","doi-asserted-by":"crossref","first-page":"619","DOI":"10.2144\/000112776","article-title":"Twenty-five years of quantitative PCR for gene expression analysis","volume":"44","author":"VanGuilder","year":"2008","journal-title":"Biotechniques"},{"key":"10.1016\/j.talanta.2026.130509_bib69","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1101\/gr.6.10.986","article-title":"Real time quantitative PCR","volume":"6","author":"Heid","year":"1996","journal-title":"Genome Res."},{"key":"10.1016\/j.talanta.2026.130509_bib70","series-title":"Encyclopedia of Food Microbiology","first-page":"344","article-title":"IDENTIFICATION METHODS | real-time PCR","author":"Rodr\u00edguez-L\u00e1zaro","year":"2014"},{"key":"10.1016\/j.talanta.2026.130509_bib71","doi-asserted-by":"crossref","first-page":"85","DOI":"10.4103\/2277-9175.127998","article-title":"Comparison of SYBR green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes","volume":"3","author":"Tajadini","year":"2014","journal-title":"Adv. Biomed. Res."},{"key":"10.1016\/j.talanta.2026.130509_bib72","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gnh101","article-title":"Investigations on DNA intercalation and surface binding by SYBR green I, its structure determination and methodological implications","volume":"32","author":"Zipper","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.talanta.2026.130509_bib73","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1383-5718(98)00172-7","article-title":"Comparison of SYBR\u00ae green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the salmonella\/mammalian microsome reverse mutation assay (Ames test)","volume":"439","author":"Singer","year":"1999","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen"},{"key":"10.1016\/j.talanta.2026.130509_bib74","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1007\/s11033-025-10763-1","article-title":"Improved genomic DNA extraction from citrus species using a modified CTAB method","volume":"52","author":"Gill","year":"2025","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.talanta.2026.130509_bib75","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s13205-016-0375-0","article-title":"An efficient protocol for isolation of inhibitor-free nucleic acids even from recalcitrant plants","volume":"6","author":"Rezadoost","year":"2016","journal-title":"3 Biotech"},{"key":"10.1016\/j.talanta.2026.130509_bib76","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1093\/clinchem\/hvaf043","article-title":"Miqe 2.0: revision of the minimum information for publication of quantitative real-time PCR experiments guidelines","volume":"71","author":"Bustin","year":"2025","journal-title":"Clin. Chem."},{"key":"10.1016\/j.talanta.2026.130509_bib77","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/0005-2795(71)90132-2","article-title":"Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin g, by means of enzyme-labelled antigen and antibody-coated tubes","volume":"251","author":"Engvall","year":"1971","journal-title":"Biochim. Biophys. Acta Protein Struct."},{"key":"10.1016\/j.talanta.2026.130509_bib78","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1365-3083.1984.tb00931.x","article-title":"Senior overviews","volume":"19","author":"Lindenmann","year":"1984","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.talanta.2026.130509_bib79","series-title":"Diagnostic Immunohistochemistry","first-page":"1","article-title":"Techniques of immunohistochemistry","author":"Taylor","year":"2011"},{"key":"10.1016\/j.talanta.2026.130509_bib80","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1094\/Phyto-69-190","article-title":"The use of enzyme-linked immunosorbent assay for detection","volume":"69","author":"Bar-Joseph","year":"1979","journal-title":"Phytopathology"},{"key":"10.1016\/j.talanta.2026.130509_bib81","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2021.114412","article-title":"A new single-chain variable fragment (scFv) antibody provides sensitive and specific detection of citrus tristeza virus","volume":"300","author":"Raeisi","year":"2022","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.talanta.2026.130509_bib82","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1094\/PDIS-11-12-1086-RE","article-title":"A new diagnostic system for detection of \u2018Candidatus Liberibacter asiaticus\u2019 infection in citrus","volume":"97","author":"Lu","year":"2013","journal-title":"Plant Dis."},{"key":"10.1016\/j.talanta.2026.130509_bib83","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1007\/s12250-016-3718-4","article-title":"Monoclonal antibody-based serological methods for detecting citrus tristeza virus in citrus groves","volume":"31","author":"Liu","year":"2016","journal-title":"Virol. Sin."},{"key":"10.1016\/j.talanta.2026.130509_bib84","doi-asserted-by":"crossref","first-page":"3414","DOI":"10.3390\/foods14193414","article-title":"Matrix interference of vegetable on enzyme-linked immunosorbent assay for parathion residue detection","volume":"14","author":"Chen","year":"2025","journal-title":"Foods"},{"key":"10.1016\/j.talanta.2026.130509_bib85","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1007\/s00253-026-13735-y","article-title":"Development of a double-monoclonal antibody sandwich ELISA for the detection of citrus tristeza virus","volume":"110","author":"Shang","year":"2026","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.talanta.2026.130509_bib86","first-page":"91","author":"Matson","year":"2023"},{"key":"10.1016\/j.talanta.2026.130509_bib87","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.trac.2018.09.027","article-title":"Paper-based immunosensors: current trends in the types and applied detection techniques","volume":"111","author":"Zhu","year":"2019","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130509_bib88","doi-asserted-by":"crossref","first-page":"9677","DOI":"10.1007\/s11033-023-08838-y","article-title":"Novel plant disease detection techniques-a brief review","volume":"50","author":"Attaluri","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.talanta.2026.130509_bib89","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1111\/pbi.13828","article-title":"Time\u2010resolved fluorescent microsphere lateral flow biosensors for rapid detection of candidatus Liberibacter asiaticus","volume":"20","author":"Su","year":"2022","journal-title":"Plant Biotechnol. J."},{"key":"10.1016\/j.talanta.2026.130509_bib90","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-77692-w","article-title":"Development of a reverse transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay (CTV-RT-RPA-LFICA) for rapid detection of citrus tristeza virus","volume":"10","author":"Ghosh","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.talanta.2026.130509_bib91","first-page":"72","article-title":"Eliminating viscosity bias in lateral flow tests, microsyst","volume":"7","author":"Kainz","year":"2021","journal-title":"Nano"},{"key":"10.1016\/j.talanta.2026.130509_bib92","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-74407-3","article-title":"Lateral flow assay sensitivity and signal enhancement via laser \u03bc-machined constrains in nitrocellulose membrane","volume":"14","author":"Khatmi","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.talanta.2026.130509_bib93","series-title":"Health and Environmental Applications of Biosensing Technologies","first-page":"1","article-title":"Fundamentals of a biosensor system","author":"Krishnan","year":"2024"},{"key":"10.1016\/j.talanta.2026.130509_bib94","doi-asserted-by":"crossref","first-page":"48918","DOI":"10.1021\/acsomega.4c07991","article-title":"Sensing the future\u2500frontiers in biosensors: exploring classifications, principles, and recent advances","volume":"9","author":"Manoharan Nair Sudha Kumari","year":"2024","journal-title":"ACS Omega"},{"key":"10.1016\/j.talanta.2026.130509_bib96","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2025.115374","article-title":"Photobioelectrochemical sensor based on WO3 modified by chloroplast as a platform for simazine detection in environmental waters","volume":"218","author":"Dias","year":"2025","journal-title":"Microchem. J."},{"key":"10.1016\/j.talanta.2026.130509_bib97","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1002\/celc.201600758","article-title":"Electrochemical biosensors in Point-of-Care devices: recent advances and future trends","volume":"4","author":"da Silva","year":"2017","journal-title":"Chemelectrochem"},{"key":"10.1016\/j.talanta.2026.130509_bib98","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.jmrt.2021.08.023","article-title":"Use of two functional monomers for a new approach to the synthesis of a magnetic molecularly imprinted polymer for ciprofloxacin","volume":"15","author":"Silva","year":"2021","journal-title":"J. Mater. Res. Technol."},{"key":"10.1016\/j.talanta.2026.130509_bib99","series-title":"Biosensors for Emerging and Re-Emerging Infectious Diseases","first-page":"1","article-title":"Biosensors: principle, fundamentals history, recent trends and applications","author":"Das","year":"2022"},{"key":"10.1016\/j.talanta.2026.130509_bib100","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.snb.2016.12.135","article-title":"Label-free electrochemical immunosensor for direct detection of citrus tristeza virus using modified gold electrode","volume":"244","author":"Haji-Hashemi","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"10.1016\/j.talanta.2026.130509_bib101","doi-asserted-by":"crossref","first-page":"5176","DOI":"10.3390\/ijms27125176","article-title":"Why sensors fail in biological samples: fouling, blocking, matrix effects and prevention solutions","volume":"27","author":"Lenar","year":"2026","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.talanta.2026.130509_bib102","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2023.1187573","article-title":"Plant impedance spectroscopy: a review of modeling approaches and applications","volume":"14","author":"Van Haeverbeke","year":"2023","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.talanta.2026.130509_bib103","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-63112-w","article-title":"Development of label-free electrochemical OMP-DNA probe biosensor as a highly sensitive system to detect of citrus huanglongbing","volume":"14","author":"Kazemzadeh-Beneh","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.talanta.2026.130509_bib104","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117986","article-title":"Magnetic bead-assisted one-pot RCA-activated CRISPR\/Cas12a electrochemiluminescence biosensor for the detection of citrus huanglongbing pathogen","volume":"290","author":"He","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2026.130509_bib105","doi-asserted-by":"crossref","DOI":"10.1016\/j.sab.2021.106218","article-title":"Application of laser-induced breakdown spectroscopy (LIBS) in environmental monitoring","volume":"181","author":"Zhang","year":"2021","journal-title":"Spectrochim. Acta Part B At. Spectrosc."},{"key":"10.1016\/j.talanta.2026.130509_bib106","doi-asserted-by":"crossref","DOI":"10.1016\/j.sab.2022.106595","article-title":"Progress in calibration-free laser-induced breakdown spectroscopy","volume":"200","author":"Hermann","year":"2023","journal-title":"Spectrochim. Acta Part B At. Spectrosc."},{"key":"10.1016\/j.talanta.2026.130509_bib107","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/s10462-024-11100-x","article-title":"Deep learning and computer vision in plant disease detection: a comprehensive review of techniques, models, and trends in precision agriculture","volume":"58","author":"Upadhyay","year":"2025","journal-title":"Artif. Intell. Rev."},{"key":"10.1016\/j.talanta.2026.130509_bib108","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2025.111849","article-title":"Advances in the machine learning construction process for citrus quality and disease detection","volume":"182","author":"Mai","year":"2026","journal-title":"Food Control"},{"key":"10.1016\/j.talanta.2026.130509_bib109","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.biosystemseng.2016.02.010","article-title":"Laser-induced fluorescence spectroscopy applied to early diagnosis of citrus Huanglongbing","volume":"144","author":"Ranulfi","year":"2016","journal-title":"Biosyst. Eng."},{"key":"10.1016\/j.talanta.2026.130509_bib110","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s00340-020-7392-8","article-title":"Comparative identification of citrus huanglongbing by analyzing leaves using laser-induced breakdown spectroscopy and near-infrared spectroscopy","volume":"126","author":"Xu","year":"2020","journal-title":"Appl. Phys. B"},{"key":"10.1016\/j.talanta.2026.130509_bib111","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1366\/14-07767","article-title":"Detection of anomalies in citrus leaves using laser-induced breakdown spectroscopy (LIBS)","volume":"69","author":"Sankaran","year":"2015","journal-title":"Appl. Spectrosc."},{"key":"10.1016\/j.talanta.2026.130509_bib112","doi-asserted-by":"crossref","DOI":"10.1016\/j.sab.2022.106561","article-title":"Reducing matrix effects on the quantification of Ca, Mg, and Fe in soybean leaf samples using calibration-free LIBS and one-point calibration","volume":"198","author":"Borduchi","year":"2022","journal-title":"Spectrochim. Acta Part B At. Spectrosc."},{"key":"10.1016\/j.talanta.2026.130509_bib113","doi-asserted-by":"crossref","first-page":"49320","DOI":"10.1039\/D5RA08452K","article-title":"Advances in surface-enhanced raman scattering applications for precision agriculture: monitoring plant health and crop quality","volume":"15","author":"Nguyen","year":"2025","journal-title":"RSC Adv."},{"key":"10.1016\/j.talanta.2026.130509_bib114","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/07352681003617285","article-title":"Plant disease severity estimated visually, by digital photography and image analysis, and by hyperspectral imaging","volume":"29","author":"Bock","year":"2010","journal-title":"CRC Crit. Rev. Plant Sci."},{"key":"10.1016\/j.talanta.2026.130509_bib115","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3390\/rs12010170","article-title":"Quantifying citrus tree health using true color UAV images","volume":"12","author":"Garza","year":"2020","journal-title":"Remote Sens. (Basel)."},{"key":"10.1016\/j.talanta.2026.130509_bib116","doi-asserted-by":"crossref","DOI":"10.1016\/j.postharvbio.2021.111627","article-title":"Detection of citrus black spot symptoms using spectral reflectance","volume":"180","author":"Xie","year":"2021","journal-title":"Postharvest Biol. Technol."},{"key":"10.1016\/j.talanta.2026.130509_bib117","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.3390\/rs11111373","article-title":"UAV-based remote sensing technique to detect citrus canker disease utilizing hyperspectral imaging and machine learning","volume":"11","author":"Abdulridha","year":"2019","journal-title":"Remote Sens. (Basel)."},{"key":"10.1016\/j.talanta.2026.130509_bib118","doi-asserted-by":"crossref","DOI":"10.1016\/j.compag.2023.108348","article-title":"Early detection of citrus anthracnose caused by Colletotrichum gloeosporioides using hyperspectral imaging","volume":"214","author":"Tang","year":"2023","journal-title":"Comput. Electron. Agric."},{"key":"10.1016\/j.talanta.2026.130509_bib119","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1080\/10798587.2015.1015772","article-title":"Diagnosis of CTV-infected leaves using hyperspectral imaging","volume":"21","author":"Guo","year":"2015","journal-title":"Intell. Autom. Soft Comput."},{"key":"10.1016\/j.talanta.2026.130509_bib120","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.compag.2017.08.001","article-title":"Identification of asymptomatic plants infected with citrus tristeza virus from a time series of leaf spectral characteristics","volume":"141","author":"Afonso","year":"2017","journal-title":"Comput. Electron. Agric."},{"key":"10.1016\/j.talanta.2026.130509_bib121","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/s13007-024-01315-y","article-title":"Quality assurance of hyperspectral imaging systems for neural network supported plant phenotyping","volume":"20","author":"Detring","year":"2024","journal-title":"Plant Methods"},{"key":"10.1016\/j.talanta.2026.130509_bib122","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1186\/s13007-025-01450-0","article-title":"Recent advances in plant disease detection: challenges and opportunities","volume":"21","author":"Shafay","year":"2025","journal-title":"Plant Methods"},{"key":"10.1016\/j.talanta.2026.130509_bib123","doi-asserted-by":"crossref","first-page":"371","DOI":"10.17660\/ActaHortic.2024.1399.46","article-title":"A simple diagnostic method for citrus greening disease based on deep learning","author":"Dong","year":"2024","journal-title":"Acta Hortic."},{"key":"10.1016\/j.talanta.2026.130509_bib124","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1007\/s10489-021-02452-w","article-title":"Citrus disease detection and classification using end-to-end anchor-based deep learning model","volume":"52","author":"Syed-Ab-Rahman","year":"2022","journal-title":"Appl. Intell."},{"key":"10.1016\/j.talanta.2026.130509_bib125","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-97159-0","article-title":"Integrating advanced deep learning techniques for enhanced detection and classification of citrus leaf and fruit diseases","volume":"15","author":"Goyal","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.talanta.2026.130509_bib126","doi-asserted-by":"crossref","DOI":"10.1002\/ecj.12472","article-title":"A simple diagnostic method for citrus greening disease with deep learning","volume":"108","author":"Dong","year":"2025","journal-title":"Electron. Commun. Jpn."},{"key":"10.1016\/j.talanta.2026.130509_bib127","doi-asserted-by":"crossref","first-page":"9643","DOI":"10.3390\/su15129643","article-title":"Image acquisition, preprocessing and classification of citrus fruit diseases: a systematic literature review","volume":"15","author":"Dhiman","year":"2023","journal-title":"Sustainability"},{"key":"10.1016\/j.talanta.2026.130509_bib128","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2025.1587869","article-title":"Integration of smart sensors and IOT in precision agriculture: trends, challenges and future prospectives","volume":"16","author":"Mansoor","year":"2025","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.talanta.2026.130509_bib129","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3390\/network5020014","article-title":"State of the art in internet of things standards and protocols for precision agriculture with an approach to semantic interoperability","volume":"5","author":"Roccatello","year":"2025","journal-title":"Network"},{"key":"10.1016\/j.talanta.2026.130509_bib130","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2022.123808","article-title":"Plant pathogenicity and associated\/related detection systems. A review","volume":"251","author":"Patel","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2026.130509_bib139","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1094\/PDIS-04-13-0465-RE","article-title":"qPCR quantification of pathogenic Guignardia citricarpa and nonpathogenic G. mangiferae in citrus","volume":"98","author":"Hu","year":"2014","journal-title":"Plant Dis."},{"key":"10.1016\/j.talanta.2026.130509_bib140","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1094\/PHYTOFR-02-21-0011-R","article-title":"Validation of quantitative and digital polymerase chain reaction assays targeting the mating types of phyllosticta citricarpa> , the causal agent of citrus black spot","volume":"1","author":"Urbina","year":"2021","journal-title":"PhytoFrontiersTM"},{"key":"10.1016\/j.talanta.2026.130509_bib141","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1007\/s10658-019-01831-x","article-title":"Multiplex real-time PCR for detection and quantification of Colletotrichum abscissum and C. gloeosporioides on citrus leaves","volume":"155","author":"Pereira","year":"2019","journal-title":"Eur. J. Plant Pathol."},{"key":"10.1016\/j.talanta.2026.130509_bib131","doi-asserted-by":"crossref","DOI":"10.1007\/s10462-026-11629-z","article-title":"Trends, challenges and future directions in deep learning for citrus leaf disease recognition: a survey","author":"Godage","year":"2026","journal-title":"Artif. Intell. Rev."},{"key":"10.1016\/j.talanta.2026.130509_bib132","doi-asserted-by":"crossref","DOI":"10.1111\/jph.70304","article-title":"Evaluation of machine learning, deep learning and spectroscopy integration for early huanglongbing detection: current limitations and future directions","volume":"174","author":"Torres\u2010Galv\u00e1n","year":"2026","journal-title":"J. Phytopathol."},{"key":"10.1016\/j.talanta.2026.130509_bib133","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202417520","article-title":"Machine\u2010learning\u2010aided advanced electrochemical biosensors","volume":"37","author":"Bocan","year":"2025","journal-title":"Adv. Mater."},{"key":"10.1016\/j.talanta.2026.130509_bib134","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1021\/acssensors.0c02254","article-title":"Consideration of sample matrix effects and \u201cBiological\u201d noise in optimizing the limit of detection of biosensors","volume":"5","author":"Masson","year":"2020","journal-title":"ACS Sens."},{"key":"10.1016\/j.talanta.2026.130509_bib135","doi-asserted-by":"crossref","first-page":"6351","DOI":"10.1039\/D1AN01148K","article-title":"Machine learning and chemometrics for electrochemical sensors: moving forward to the future of analytical chemistry","volume":"146","author":"Puthongkham","year":"2021","journal-title":"Analyst"},{"key":"10.1016\/j.talanta.2026.130509_bib136","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.3390\/s24123855","article-title":"Strategies to enrich electrochemical sensing data with analytical relevance for machine learning applications: a focused review","volume":"24","author":"Kang","year":"2024","journal-title":"Sensors"},{"key":"10.1016\/j.talanta.2026.130509_bib138","doi-asserted-by":"crossref","first-page":"102964","DOI":"10.1109\/ACCESS.2025.3578751","article-title":"AIoT-Enabled data management for smart agriculture: a comprehensive review on emerging technologies","volume":"13","author":"Luo","year":"2025","journal-title":"IEEE Access"},{"key":"10.1016\/j.talanta.2026.130509_bib142","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2025.1674574","article-title":"Exploring the advances of biosensing technology for the detection of plant pathogens in sustainable agriculture","volume":"13","author":"Shikha","year":"2026","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.talanta.2026.130509_bib143","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2025.1516723","article-title":"Portable solutions for plant pathogen diagnostics: development, usage, and future potential","volume":"16","author":"Yadav","year":"2025","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.talanta.2026.130509_bib144","unstructured":"National Aeronautics and Space Administration, Technology Readiness Levels, (n.d.). https:\/\/www.nasa.gov\/directorates\/somd\/space-communications-navigation-program\/technology-readiness-levels\/(accessed August 3, 2026)."},{"key":"10.1016\/j.talanta.2026.130509_bib145","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e29930","article-title":"Adaptation of the technology readiness levels for impact assessment in implementation sciences: the TRL-IS checklist","volume":"10","author":"Salvador-Carulla","year":"2024","journal-title":"Heliyon"},{"key":"10.1016\/j.talanta.2026.130509_bib146","unstructured":"Bioingentech, Phyllosticta citricarpa PCR kit, (n.d.). https:\/\/kitpcr.com\/pcr-kit\/phyllosticta-citricarpa-detection-kits\/(accessed August 3, 2026)."},{"key":"10.1016\/j.talanta.2026.130509_bib147","unstructured":"Bioingentech, Citrus leprosis virus C, (n.d.). https:\/\/kitpcr.com\/pcr-kit\/citrus-leprosis-virus-c-detection-kits\/(accessed August 3, 2026)."},{"key":"10.1016\/j.talanta.2026.130509_bib148","unstructured":"I. Agdia, ELISA reagent set for citrus tristeza virus (CTV), (n.d.). https:\/\/orders.agdia.com\/agdia-set-ctv-alkphos-sra-78900 (accessed August 3, 2026)."},{"key":"10.1016\/j.talanta.2026.130509_bib149","unstructured":"I. Agdia, ImmunoStrip\u00ae for Xanthomonas axonopodis pv. citri (Xac), (n.d.). https:\/\/orders.agdia.com\/agdia-immunostrip-for-xac-isk-92200 (accessed August 3, 2026)."},{"key":"10.1016\/j.talanta.2026.130509_bib150","unstructured":"I. Agdia, Amplifyrp\u00ae XRT for Citrus Greening (HLB), (n.d.). https:\/\/orders.agdia.com\/amplifyrp-xrt-for-hlb-xcs-78000 (accessed August 3, 2026)."},{"key":"10.1016\/j.talanta.2026.130509_bib151","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2025.1570318","article-title":"Nanomaterial-based biosensors: a new frontier in plant pathogen detection and plant disease management","volume":"13","author":"Narware","year":"2025","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.talanta.2026.130509_bib152","article-title":"Early detection of plant pathogens in the asymptomatic phase: a scoping review of hyperspectral imaging combined with machine learning","volume":"14","author":"Wang","year":"2026","journal-title":"Smart Agric. Technol."},{"key":"10.1016\/j.talanta.2026.130509_bib153","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3390\/jimaging11100326","article-title":"A review on the detection of plant disease using machine learning and deep learning approaches","volume":"11","author":"Nyawose","year":"2025","journal-title":"J. Imaging"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914026011653?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914026011653?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T21:37:19Z","timestamp":1793137039000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914026011653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":147,"alternative-id":["S0039914026011653"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2026.130509","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Analytical advances in citrus disease detection: Conventional molecular assays and emerging analytical technologies","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2026.130509","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"130509"}}