{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:08:37Z","timestamp":1790647717874,"version":"4.1.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Topics in Companion Animal Medicine"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.tcam.2022.100735","type":"journal-article","created":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T03:50:44Z","timestamp":1666237844000},"page":"100735","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["An Improved Point-of-Care ELISA for the Diagnosis of Anaplasmosis and Ehrlichiosis During the Acute Phase of Tick-Borne Infections in Dogs"],"prefix":"10.1016","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8165-5361","authenticated-orcid":false,"given":"Melissa J.","family":"Beall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine A.","family":"Mainville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Arguello-Marin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevieve","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Lemieux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Saucier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendon","family":"Thatcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward B.","family":"Breitschwerdt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah A.","family":"Cohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara A.","family":"Qurollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramaswamy","family":"Chandrashekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tcam.2022.100735_bib0001","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s13071-017-2184-7","article-title":"Canine infection with Borrelia burgdorferi, Dirofilaria immitis, Anaplasma spp. and Ehrlichia spp. in Canada","volume":"10","author":"Herrin","year":"2017","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.tcam.2022.100735_bib0002","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1089\/vbz.2014.1661","article-title":"Confirmation of Borrelia burgdorferi sensu stricto and Anaplasma phagocytophilum in Ixodes scapularis, Southwestern Virginia","volume":"14","author":"Herrin","year":"2014","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"10","key":"10.1016\/j.tcam.2022.100735_bib0003","article-title":"Canine infection with Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp., and Ehrlichia spp. in the United States, 2013-2019","volume":"14","author":"Little","year":"2021","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.tcam.2022.100735_bib0004","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1089\/vbz.2005.5.101","article-title":"The dog as a sentinel for human infection: prevalence of Borrelia burgdorferi C6 antibodies in dogs from southeastern and mid-Atlantic States","volume":"5","author":"Duncan","year":"2005","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.tcam.2022.100735_bib0005","doi-asserted-by":"crossref","first-page":"80","DOI":"10.2460\/javma.245.1.80","article-title":"Performance of a commercially available in-clinic ELISA for detection of antibodies against Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis, and Ehrlichia ewingii and Dirofilaria immitis antigen in dogs","volume":"245","author":"Stillman","year":"2014","journal-title":"J Am Vet Med Assoc"},{"key":"10.1016\/j.tcam.2022.100735_bib0006","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.2460\/ajvr.71.12.1443","article-title":"Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs","volume":"71","author":"Chandrashekar","year":"2010","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.tcam.2022.100735_bib0007","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetpar.2008.10.093","article-title":"Prevalence and geographic distribution of Dirofilaria immitis, Borrelia burgdorferi, Ehrlichia canis, and Anaplasma phagocytophilum in dogs in the United States: results of a national clinic-based serologic survey","volume":"160","author":"Bowman","year":"2009","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.tcam.2022.100735_bib0008","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1186\/1756-3305-7-257","article-title":"Canine infection with Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp., and Ehrlichia spp. in the United States, 2010-2012","volume":"7","author":"Little","year":"2014","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.tcam.2022.100735_bib0009","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1756-3305-3-33","article-title":"Experimental infection and co-infection of dogs with Anaplasma platys and Ehrlichia canis: hematologic, serologic and molecular findings","volume":"3","author":"Gaunt","year":"2010","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.tcam.2022.100735_bib0010","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1089\/vbz.2009.0262","article-title":"Comparative strain analysis of Anaplasma phagocytophilum infection and clinical outcomes in a canine model of granulocytic anaplasmosis","volume":"11","author":"Scorpio","year":"2011","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.tcam.2022.100735_bib0011","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.tvjl.2014.08.018","article-title":"Detection of antibodies against Anaplasma phagocytophilum in dogs using an automated fluorescence based system","volume":"202","author":"Moroff","year":"2014","journal-title":"Vet J"},{"key":"10.1016\/j.tcam.2022.100735_bib0012","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1177\/1040638714534849","article-title":"Use of an automated system for detection of canine serum antibodies against Ehrlichia canis glycoprotein 36","volume":"26","author":"Moroff","year":"2014","journal-title":"J Vet Diagn Invest"},{"key":"10.1016\/j.tcam.2022.100735_bib0013","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.tvjl.2017.06.005","article-title":"Serologic responses to peptides of Anaplasma phagocytophilum and Borrelia burgdorferi in dogs infested with wild-caught Ixodes scapularis","volume":"226","author":"Chandrashekar","year":"2017","journal-title":"Vet J"},{"key":"10.1016\/j.tcam.2022.100735_bib0014","series-title":"25th World Association for the Advancement of Veterinary Parasitology","article-title":"Evaluation of species-specific peptides for early detection of antibodies to canine monocytic ehrlichiosis and anaplasmosis abstract","author":"Thatcher","year":"2015"},{"key":"10.1016\/j.tcam.2022.100735_bib0015","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1128\/CVI.00361-06","article-title":"Enzyme-linked immunosorbent assay with conserved immunoreactive glycoproteins gp36 and gp19 has enhanced sensitivity and provides species-specific immunodiagnosis of Ehrlichia canis infection","volume":"14","author":"Cardenas","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.tcam.2022.100735_bib0016","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1128\/IAI.01494-06","article-title":"Identification of a glycosylated Ehrlichia canis 19-kilodalton major immunoreactive protein with a species-specific serine-rich glycopeptide epitope","volume":"75","author":"McBride","year":"2007","journal-title":"Infect Immun"},{"key":"10.1016\/j.tcam.2022.100735_bib0017","series-title":"26th Annual ACVIM Forum","first-page":"781","article-title":"Experimental Ehrlichia canis infection in dogs: antibody responses pre- and post-treatment abstract","volume":"22","author":"Bradley","year":"2008"},{"key":"10.1016\/j.tcam.2022.100735_bib0018","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.2460\/ajvr.1988.49.07.1014","article-title":"Anemia of inflammation in dogs infected with Ehrlichia platys","volume":"49","author":"Baker","year":"1988","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.tcam.2022.100735_bib0019","doi-asserted-by":"crossref","first-page":"916","DOI":"10.2460\/javma.1986.189.08.916","article-title":"Uveitis associated with Ehrlichia platys infection in a dog","volume":"189","author":"Glaze","year":"1986","journal-title":"J Am Vet Med Assoc"},{"key":"10.1016\/j.tcam.2022.100735_bib0020","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/s13071-020-04118-x","article-title":"Predominant risk factors for tick-borne co-infections in hunting dogs from the USA","volume":"13","author":"Mahachi","year":"2020","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.tcam.2022.100735_bib0021","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/jvim.15675","article-title":"Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii","volume":"34","author":"Lashnits","year":"2020","journal-title":"J Vet Intern Med"},{"key":"10.1016\/j.tcam.2022.100735_bib0022","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1089\/vbz.2014.1592","article-title":"Regional seroreactivity and vector-borne disease co-exposures in dogs in the United States from 2004-2010: utility of canine surveillance","volume":"14","author":"Yancey","year":"2014","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.tcam.2022.100735_bib0023","article-title":"A serological survey of tick-borne pathogens in dogs in North America and the Caribbean as assessed by Anaplasma phagocytophilum, A. platys, Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia burgdorferi species-specific peptides","volume":"4","author":"Qurollo","year":"2014","journal-title":"Infect Ecol Epidemiol"},{"key":"10.1016\/j.tcam.2022.100735_bib0024","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.2460\/ajvr.71.10.1195","article-title":"Evaluation of peptide- and recombinant protein-based assays for detection of anti-Ehrlichia ewingii antibodies in experimentally and naturally infected dogs. corrected","volume":"71","author":"O'Connor","year":"2010","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.tcam.2022.100735_bib0025","doi-asserted-by":"crossref","first-page":"71","DOI":"10.2460\/ajvr.82.1.71","article-title":"Comparison of Anaplasma and Ehrlichia species-specific peptide ELISAs with whole organism-based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs","volume":"82","author":"Qurollo","year":"2021","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.tcam.2022.100735_bib0026","series-title":"R: A Language and Environment for Statistical Computing (version 4.1.0)","author":"Core Team","year":"2021"},{"key":"10.1016\/j.tcam.2022.100735_bib0027","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1128\/JCM.37.3.558-564.1999","article-title":"Serologic testing for human granulocytic ehrlichiosis at a national referral center","volume":"37","author":"Comer","year":"1999","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.tcam.2022.100735_bib0028","doi-asserted-by":"crossref","first-page":"124","DOI":"10.4269\/ajtmh.2011.10-0613","article-title":"Increasing incidence of Ehrlichia chaffeensis and Anaplasma phagocytophilum in the United States, 2000-2007","volume":"85","author":"Dahlgren","year":"2011","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.tcam.2022.100735_bib0029","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1756-3305-5-29","article-title":"Seroprevalence of Ehrlichia canis, Ehrlichia chaffeensis and Ehrlichia ewingii in dogs in North America","volume":"5","author":"Beall","year":"2012","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.tcam.2022.100735_bib0030","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1111\/jvim.15354","article-title":"Clinicopathological findings in 41 dogs (2008-2018) naturally infected with Ehrlichia ewingii","volume":"33","author":"Qurollo","year":"2019","journal-title":"J Vet Intern Med"},{"key":"10.1016\/j.tcam.2022.100735_bib0031","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.vetmic.2014.08.006","article-title":"Development of antibodies to and PCR detection of Ehrlichia spp. in dogs following natural tick exposure","volume":"173","author":"Starkey","year":"2014","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.tcam.2022.100735_bib0032","doi-asserted-by":"crossref","DOI":"10.1016\/j.tvjl.2021.105676","article-title":"Comparative analysis of antibody responses to outer surface protein (Osp)A and OspC in dogs vaccinated with Lyme disease vaccines","volume":"273","author":"Camire","year":"2021","journal-title":"Vet J"},{"key":"10.1016\/j.tcam.2022.100735_bib0033","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.vetimm.2010.12.003","article-title":"A fluorescent bead-based multiplex assay for the simultaneous detection of antibodies to Borrelia burgdorferi outer surface proteins in canine serum","volume":"140","author":"Wagner","year":"2011","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.tcam.2022.100735_bib0034","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1128\/CVI.00373-08","article-title":"Bacterin that induces anti-OspA and anti-OspC borreliacidal antibodies provides a high level of protection against canine Lyme disease","volume":"16","author":"LaFleur","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.tcam.2022.100735_bib0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.jvacx.2020.100079","article-title":"VANGUARD(R)crLyme: A next generation Lyme disease vaccine that prevents Borrelia burgdorferi infection in dogs","volume":"6","author":"Marconi","year":"2020","journal-title":"Vaccine X"},{"key":"10.1016\/j.tcam.2022.100735_bib0036","doi-asserted-by":"crossref","DOI":"10.1016\/j.tcam.2019.100358","article-title":"Borrelia burgdorferi antibody test results in dogs administered 4 different vaccines","volume":"37","author":"Stillman","year":"2019","journal-title":"Top Companion Anim Med"},{"key":"10.1016\/j.tcam.2022.100735_bib0037","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00628-19","article-title":"Antibodies to Borrelia turicatae in experimentally infected dogs cross-react with Borrelia burgdorferi serologic assays","volume":"57","author":"Gettings","year":"2019","journal-title":"J Clin Microbiol"}],"container-title":["Topics in Companion Animal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1938973622001088?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1938973622001088?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T18:40:31Z","timestamp":1761849631000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1938973622001088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":37,"alternative-id":["S1938973622001088"],"URL":"https:\/\/doi.org\/10.1016\/j.tcam.2022.100735","relation":{},"ISSN":["1938-9736"],"issn-type":[{"value":"1938-9736","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An Improved Point-of-Care ELISA for the Diagnosis of Anaplasmosis and Ehrlichiosis During the Acute Phase of Tick-Borne Infections in Dogs","name":"articletitle","label":"Article Title"},{"value":"Topics in Companion Animal Medicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tcam.2022.100735","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 IDEXX Laboratories, Inc. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"100735"}}