{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T01:51:42Z","timestamp":1777600302617,"version":"3.51.4"},"reference-count":64,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordination of Higher Education Personnel Improvement","doi-asserted-by":"publisher","award":["2015 - 0774\/2017"],"award-info":[{"award-number":["2015 - 0774\/2017"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-00524-17"],"award-info":[{"award-number":["APQ-00524-17"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["RED-00132-16"],"award-info":[{"award-number":["RED-00132-16"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007374","name":"Federal University of Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007374","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["406402\/2018-3"],"award-info":[{"award-number":["406402\/2018-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Topics in Companion Animal Medicine"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.tcam.2025.100981","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T12:13:59Z","timestamp":1746533639000},"page":"100981","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Metagenomic and 16S rRNA analysis of culture-negative uterine samples identifies Brucella spp. in a female dog with pyometra"],"prefix":"10.1016","volume":"66","author":[{"given":"Rafael Gariglio Clark","family":"Xavier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarissa Helena","family":"Santana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasmin Gon\u00e7alves","family":"de Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Dias de Oliveira","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tales Fernando","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasco","family":"Azevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via Figueira","family":"Aburjaile","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Maria Seles","family":"Dorneles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato Lima","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0074-2488","authenticated-orcid":false,"given":"Rodrigo Ot\u00e1vio Silveira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.tcam.2025.100981_bib0001","doi-asserted-by":"crossref","unstructured":"Hagman R. Pyometra in small animals. Vet clin small anim pract. 2018;48(4):639-661. doi:10.1016\/j.cvsm.2018.03.001.","DOI":"10.1016\/j.cvsm.2018.03.001"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0002","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12917-016-0924-0","article-title":"Closed cervix is associated with more severe illness in dogs with pyometra","volume":"13","author":"Jitpean","year":"2017","journal-title":"BMC Vet Res"},{"key":"10.1016\/j.tcam.2025.100981_bib0003","doi-asserted-by":"crossref","unstructured":"Hagman R. Pyometra in small animals 2.0. Vet Clin North Am small Anim Pract. 2022;52(3):631-657. doi:10.1016\/j.cvsm.2022.01.004.","DOI":"10.1016\/j.cvsm.2022.01.004"},{"issue":"2","key":"10.1016\/j.tcam.2025.100981_bib0004","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.rvsc.2008.07.018","article-title":"Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs","volume":"86","author":"Siqueira","year":"2009","journal-title":"Res Vet Sci"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0005","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.vetmic.2013.02.028","article-title":"The role of type 1, P and S fimbriae in binding of Escherichia coli to the canine endometrium","volume":"164","author":"Krekeler","year":"2013","journal-title":"Vet Microbiol"},{"issue":"5","key":"10.1016\/j.tcam.2025.100981_bib0006","first-page":"245","article-title":"Characterization of Escherichia coli in dogs with Pyometra and the influence of diet on the intestinal colonization of extraintestinal pathogenic E. coli (ExPEC)","volume":"9","author":"Xavier","year":"2022","journal-title":"Vet Sci"},{"key":"10.1016\/j.tcam.2025.100981_bib0007","doi-asserted-by":"crossref","unstructured":"Hagman R. Pyometra in small animals 3.0. Vet Clin North Am Small Anim Pract. Published online June 1, 2023. doi:10.1016\/j.cvsm.2023.04.009.","DOI":"10.1016\/j.cvsm.2023.04.009"},{"issue":"21","key":"10.1016\/j.tcam.2025.100981_bib0008","doi-asserted-by":"crossref","first-page":"3310","DOI":"10.3390\/ani13213310","article-title":"Canine Pyometra: a short review of current advances","volume":"13","author":"Xavier","year":"2023","journal-title":"Animals"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0009","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1590\/S1517-83822008000300012","article-title":"Benites NR. Microbiological and histopathological aspects of canine pyometra","volume":"39","author":"Coggan","year":"2008","journal-title":"Braz J Microbiol Publ Braz Soc Microbiol"},{"issue":"7","key":"10.1016\/j.tcam.2025.100981_bib0010","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.14202\/vetworld.2022.1804-1813","article-title":"First report on clinical aspects, blood profiles, bacterial isolation, antimicrobial susceptibility, and histopathology in canine pyometra in Thailand","volume":"15","author":"Lansubsakul","year":"2022","journal-title":"Vet World"},{"key":"10.1016\/j.tcam.2025.100981_bib0011","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.theriogenology.2023.10.007","article-title":"Association between bacterial pathogenicity, endometrial histological changes and clinical prognosis in canine pyometra","volume":"214","author":"Xavier","year":"2024","journal-title":"Theriogenology"},{"issue":"2","key":"10.1016\/j.tcam.2025.100981_bib0012","doi-asserted-by":"crossref","first-page":"128","DOI":"10.4110\/in.2017.17.2.128","article-title":"Sterile pyometra in two dogs","volume":"17","author":"Yoon","year":"2017","journal-title":"Immune Netw"},{"issue":"9","key":"10.1016\/j.tcam.2025.100981_bib0013","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1128\/aem.62.9.3350-3354.1996","article-title":"Confirmational identification of Escherichia coli, a comparison of genotypic and phenotypic assays for glutamate decarboxylase and beta-d-glucuronidase","volume":"62","author":"McDaniels","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.tcam.2025.100981_bib0014","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.01492","article-title":"Use of MALDI-TOF mass spectrometry for the fast identification of gram-positive fish pathogens","volume":"8","author":"Assis","year":"2017","journal-title":"Front Microbiol"},{"issue":"6","key":"10.1016\/j.tcam.2025.100981_bib0015","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0269422","article-title":"Occurrence and characterization of methicillin-resistant Staphylococcus spp. In diseased dogs in Brazil","volume":"17","author":"Viegas","year":"2022","journal-title":"PLOS ONE"},{"issue":"2","key":"10.1016\/j.tcam.2025.100981_bib0016","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4103\/2305-0500.254645","article-title":"Review on canine pyometra, oxidative stress and current trends in diagnostics","volume":"8","author":"Rautela","year":"2019","journal-title":"Asian Pac J Reprod"},{"key":"10.1016\/j.tcam.2025.100981_bib0017","first-page":"167","article-title":"Canine pyometra: interferences of age and type in blood count and serum biochemistry","author":"dos","year":"2021","journal-title":"Rev Bras Ci\u00eanc Veterin\u00e1ria. Published online"},{"issue":"24","key":"10.1016\/j.tcam.2025.100981_bib0018","first-page":"659","article-title":"Uterine bacterial flora and uterine lesions in bitches with cystic endometrial hyperplasia (pyometra)","volume":"143","author":"Dhaliwal","year":"1998","journal-title":"Vet Rec"},{"issue":"6","key":"10.1016\/j.tcam.2025.100981_bib0019","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1111\/j.1939-1676.2010.0603.x","article-title":"Escherichia coli pyometra induces transient glomerular and tubular dysfunction in dogs","volume":"24","author":"Maddens","year":"2010","journal-title":"J Vet Intern Med"},{"key":"10.1016\/j.tcam.2025.100981_bib0020","doi-asserted-by":"crossref","unstructured":"Hamidi A., Sylejmani D., Robaj A. Occurrence and antimicrobial susceptibility ofbacterial agents of canine pyometra. Indian J Anim Res. 2016;(OF). doi:10.18805\/ijar.v0iOF.6830.","DOI":"10.18805\/ijar.v0iOF.6830"},{"issue":"2","key":"10.1016\/j.tcam.2025.100981_bib0021","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1128\/jcm.33.2.445-454.1995","article-title":"Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice","volume":"33","author":"Fox","year":"1995","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.tcam.2025.100981_bib0022","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0057923","article-title":"The variability of the 16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses","volume":"8","author":"V\u011btrovsk\u00fd","year":"2013","journal-title":"PLOS ONE"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0023","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/npjbiofilms.2016.4","article-title":"A perspective on 16S rRNA operational taxonomic unit clustering using sequence similarity","volume":"2","author":"Nguyen","year":"2016","journal-title":"Npj Biofilms Microbiomes"},{"issue":"10","key":"10.1016\/j.tcam.2025.100981_bib0024","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1128\/JCM.00837-08","article-title":"Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains","volume":"46","author":"L\u00f3pez-Go\u00f1i","year":"2008","journal-title":"J Clin Microbiol"},{"issue":"5","key":"10.1016\/j.tcam.2025.100981_bib0025","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat Methods"},{"issue":"15","key":"10.1016\/j.tcam.2025.100981_bib0026","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinforma Oxf Engl"},{"issue":"16","key":"10.1016\/j.tcam.2025.100981_bib0027","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/bioinformatics\/btr381","article-title":"UCHIME improves sensitivity and speed of chimera detection","volume":"27","author":"Edgar","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1016\/j.tcam.2025.100981_bib0028","doi-asserted-by":"crossref","first-page":"e2584","DOI":"10.7717\/peerj.2584","article-title":"VSEARCH: a versatile open source tool for metagenomics","volume":"4","author":"Rognes","year":"2016","journal-title":"PeerJ"},{"issue":"D1","key":"10.1016\/j.tcam.2025.100981_bib0029","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0030","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1128\/JCM.36.1.157-160.1998","article-title":"Detection of Porphyromonas gingivalis from Saliva by PCR by using a simple sample-processing method","volume":"36","author":"M\u00e4tt\u00f6","year":"1998","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0031","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetmic.2004.12.018","article-title":"Pigmented-anaerobic bacteria associated with canine periodontitis","volume":"106","author":"Hardham","year":"2005","journal-title":"Vet Microbiol"},{"issue":"9","key":"10.1016\/j.tcam.2025.100981_bib0032","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1007\/s00467-007-0531-1","article-title":"Urinary tract infection caused by Haemophilus influenzae and Haemophilus parainfluenzae in children","volume":"22","author":"Hansson","year":"2007","journal-title":"Pediatr Nephrol Berl Ger"},{"issue":"12","key":"10.1016\/j.tcam.2025.100981_bib0033","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0113744","article-title":"Early canine plaque biofilms: characterization of key bacterial interactions involved in initial colonization of enamel","volume":"9","author":"Holcombe","year":"2014","journal-title":"PLOS ONE"},{"key":"10.1016\/j.tcam.2025.100981_bib0034","article-title":"Microbiological and molecular detection of canicola (Haemophilus) haemoglobinophilus from the urine of a dog","volume":"28","author":"Karthikeyan","year":"2022","journal-title":"INDIAN J Anim Health. Published online November"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.13005\/bbra\/3197","article-title":"The impact of dogs oral microbiota on Human health: a review","volume":"21","author":"AL-Amrah","year":"2024","journal-title":"Biosci Biotechnol Res Asia"},{"key":"10.1016\/j.tcam.2025.100981_bib0036","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.theriogenology.2022.11.003","article-title":"A study on the correlation between intrauterine microbiota and uterine pyogenesis in dogs","volume":"196","author":"Zheng","year":"2023","journal-title":"Theriogenology"},{"issue":"7","key":"10.1016\/j.tcam.2025.100981_bib0037","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/nrmicro2381","article-title":"Oral multispecies biofilm development and the key role of cell\u2013cell distance","volume":"8","author":"Kolenbrander","year":"2010","journal-title":"Nat Rev Microbiol"},{"issue":"2","key":"10.1016\/j.tcam.2025.100981_bib0038","first-page":"161","article-title":"Comparison of serum amyloid A and C-reactive protein as diagnostic markers of systemic inflammation in dogs","volume":"55","author":"Christensen","year":"2014","journal-title":"Can Vet J"},{"key":"10.1016\/j.tcam.2025.100981_bib0039","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.micpath.2017.11.001","article-title":"Oral pathogenesis of Aggregatibacter actinomycetemcomitans","volume":"113","author":"Gholizadeh","year":"2017","journal-title":"Microb Pathog"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0040","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-97057-1","article-title":"Porphyromonas spp., Fusobacterium spp., and Bacteroides spp. Dominate microbiota in the course of macropod progressive periodontal disease","volume":"11","author":"Yip","year":"2021","journal-title":"Sci Rep"},{"issue":"23","key":"10.1016\/j.tcam.2025.100981_bib0041","doi-asserted-by":"crossref","first-page":"3594","DOI":"10.3390\/ani13233594","article-title":"Tozon N. A comparison of the oral microbiota in healthy dogs and dogs with oral tumors","volume":"13","author":"Lisjak","year":"2023","journal-title":"Animals"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0042","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s10096-023-04734-2","article-title":"Clinical characteristics and antimicrobial susceptibility of Fusobacterium species isolated over 10 years at a Japanese university hospital","volume":"43","author":"Mori","year":"2024","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"4","key":"10.1016\/j.tcam.2025.100981_bib0043","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.anaerobe.2005.01.007","article-title":"Fusobacterium necrophorum infections in animals: pathogenesis and pathogenic mechanisms","volume":"11","author":"Nagaraja","year":"2005","journal-title":"Anaerobe"},{"key":"10.1016\/j.tcam.2025.100981_bib0044","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1600-0757.1999.tb00161.x","article-title":"Virulence factors of Actinobacillus actinomycetemcomitans","volume":"20","author":"Fives-Taylor","year":"1999","journal-title":"Periodontol 2000"},{"issue":"12","key":"10.1016\/j.tcam.2025.100981_bib0045","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1099\/0022-1317-51-12-1013","article-title":"Actinobacillus actinomycetemcomitans","volume":"51","author":"Henderson","year":"2002","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.tcam.2025.100981_bib0046","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2021.727641","article-title":"Brucella suis seroprevalence and associated risk factors in dogs in Eastern Australia, 2016 to 2019","volume":"8","author":"Kneipp","year":"2021","journal-title":"Front Vet Sci"},{"key":"10.1016\/j.tcam.2025.100981_bib0047","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jvc.2024.02.009","article-title":"Aorto-left atrial fistula secondary to aortic infective endocarditis in a dog with a bicuspid aortic valve","volume":"53","author":"Carrillo","year":"2024","journal-title":"J Vet Cardiol"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0048","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0378-1119(88)90540-9","article-title":"The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein","volume":"63","author":"Mayfield","year":"1988","journal-title":"Gene"},{"key":"10.1016\/j.tcam.2025.100981_bib0049","first-page":"271","article-title":"Detection of brucella melitensis and brucella abortus by DNA amplification","volume":"95","author":"Baily","year":"1992","journal-title":"J Trop Med Hyg"},{"key":"10.1016\/j.tcam.2025.100981_bib0050","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1590\/S1517-83822014005000048","article-title":"Molecular detection of brucella species in patients suspicious of brucellosis from Zanjan, Iran","volume":"45","author":"Garshasbi","year":"2014","journal-title":"Braz J Microbiol"},{"key":"10.1016\/j.tcam.2025.100981_bib0051","doi-asserted-by":"crossref","DOI":"10.1016\/j.mgene.2020.100683","article-title":"Molecular survey of BCSP31 and IS711 using PCR assays in detection of brucella spp. In raw milk","volume":"24","author":"Satei","year":"2020","journal-title":"Meta Gene"},{"key":"10.1016\/j.tcam.2025.100981_bib0052","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2021.106182","article-title":"Comparative study of IS711 and bcsp31-based polymerase chain reaction (PCR) for the diagnosis of human brucellosis in whole blood and serum samples","volume":"183","author":"Becker","year":"2021","journal-title":"J Microbiol Methods"},{"issue":"1","key":"10.1016\/j.tcam.2025.100981_bib0053","doi-asserted-by":"crossref","first-page":"31","DOI":"10.51585\/gjvr.2023.1.0050","article-title":"Genetic comparison of brucella spp. And ochrobactrum spp. Erroneously included into the genus Brucella confirms separate genera","volume":"3","author":"Holzer","year":"2023","journal-title":"Ger J Vet Res"},{"key":"10.1016\/j.tcam.2025.100981_bib0054","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.106345","article-title":"Environmental risk characterization and ecological process determination of bacterial antibiotic resistome in lake sediments","volume":"147","author":"Chen","year":"2021","journal-title":"Environ Int"},{"key":"10.1016\/j.tcam.2025.100981_bib0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2021.104865","article-title":"Emerging diversity and ongoing expansion of the genus brucella","volume":"92","author":"Whatmore","year":"2021","journal-title":"Infect Genet Evol"},{"key":"10.1016\/j.tcam.2025.100981_bib0056","doi-asserted-by":"crossref","unstructured":"Morgan W.J.B. Techniques for the Brucellosis laboratory: G.G. Alton, L.M. Jones, R.D. Angus & J.M. Verger Versailles Cedex: INRA Publications. 1988. 192pp. Ff 195. Br Vet J. 2007;146(2):188. doi:10.1016\/0007-1935(90)90017-W.","DOI":"10.1016\/0007-1935(90)90017-W"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0057","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.theriogenology.2006.04.011","article-title":"Canine brucellosis: outbreaks and compliance","volume":"66","author":"Hollett","year":"2006","journal-title":"Theriogenology"},{"issue":"12","key":"10.1016\/j.tcam.2025.100981_bib0058","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.3201\/eid1712.111127","article-title":"Brucella suis infection in dogs","volume":"17","author":"Ramamoorthy","year":"2011","journal-title":"Emerg Infect Dis"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0059","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1111\/tbed.12632","article-title":"Brucella canis infection in dogs from commercial breeding kennels in Brazil","volume":"64","author":"Keid","year":"2017","journal-title":"Transbound Emerg Dis"},{"issue":"5","key":"10.1016\/j.tcam.2025.100981_bib0060","doi-asserted-by":"crossref","first-page":"e27","DOI":"10.1111\/tbed.12535","article-title":"Isolation of brucella abortus from a dog and a cat confirms their biological role in re-emergence and dissemination of bovine brucellosis on dairy farms","volume":"64","author":"Wareth","year":"2017","journal-title":"Transbound Emerg Dis"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0061","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.theriogenology.2008.04.035","article-title":"Bacterial and protozoal causes of pregnancy loss in the bitch and queen","volume":"70","author":"Pretzer","year":"2008","journal-title":"Theriogenology"},{"key":"10.1016\/j.tcam.2025.100981_bib0062","doi-asserted-by":"crossref","DOI":"10.1016\/j.cimid.2020.101554","article-title":"Brucella melitensis infection in dog: a critical issue in the control of brucellosis in ruminant farms","volume":"73","author":"Alamian","year":"2020","journal-title":"Comp Immunol Microbiol Infect Dis"},{"issue":"3","key":"10.1016\/j.tcam.2025.100981_bib0063","doi-asserted-by":"crossref","first-page":"1","DOI":"10.51585\/gjvr.2023.3.0056","article-title":"Development of antigen-capture ELISA using monoclonal antibodies for the detection of brucellae in milk","volume":"3","author":"G\u00fcrbilek","year":"2023","journal-title":"Ger J Vet Res"},{"issue":"9\u201310","key":"10.1016\/j.tcam.2025.100981_bib0064","first-page":"487","article-title":"Laboratory-based diagnosis of brucellosis\u2013a review of the literature. Part I: techniques for direct detection and identification of brucella spp","volume":"49","author":"Al Dahouk","year":"2003","journal-title":"Clin Lab"}],"container-title":["Topics in Companion Animal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1938973625000340?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1938973625000340?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T10:33:24Z","timestamp":1762598004000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1938973625000340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":64,"alternative-id":["S1938973625000340"],"URL":"https:\/\/doi.org\/10.1016\/j.tcam.2025.100981","relation":{},"ISSN":["1938-9736"],"issn-type":[{"value":"1938-9736","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Metagenomic and 16S rRNA analysis of culture-negative uterine samples identifies Brucella spp. in a female dog with pyometra","name":"articletitle","label":"Article Title"},{"value":"Topics in Companion Animal Medicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tcam.2025.100981","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"100981"}}