{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:48:51Z","timestamp":1792147731093,"version":"4.4.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Topics in Companion Animal Medicine"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.tcam.2026.101106","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:13:45Z","timestamp":1791558825000},"page":"101106","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Biological response stability of refrigerated cosacthen after opening and non-hormonal effects of ACTH stimulation testing in healthy dogs"],"prefix":"10.1016","volume":"73","author":[{"given":"Milena Cleff","family":"de Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3289-0157","authenticated-orcid":false,"given":"Ta\u00eds Bock","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda de Lucena","family":"Gouv\u00eaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Moura","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1819-0653","authenticated-orcid":false,"given":"Luciano","family":"Trevizan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7117-5284","authenticated-orcid":false,"given":"\u00c1lan Gomes","family":"P\u00f6ppl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"n.1","key":"10.1016\/j.tcam.2026.101106_bib0001","first-page":"2","article-title":"Diagnosis of hyperadrenocorticism in dogs","volume":"v.22","author":"Peterson","year":"2007","journal-title":"Clin Tech Small Anim Prher assistance with the translation of this manuscript, CAPES and CNPq for their financial support, and the VETEX Laboratory for its assistance with sample processing.act"},{"issue":"n.6","key":"10.1016\/j.tcam.2026.101106_bib0002","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1111\/jvim.12192","article-title":"Diagnosis of spontaneous canine hyperadrenocorticism: 2012 ACVIM consensus statement (small animal)","volume":"v.27","author":"Behrend","year":"2013","journal-title":"J Vet Intern Med"},{"key":"10.1016\/j.tcam.2026.101106_bib0003","series-title":"Endocrinologia Canina E Felina","first-page":"cap. 10.","article-title":"Hiperadrenocorticismo canino","author":"Behrend","year":"2015"},{"key":"10.1016\/j.tcam.2026.101106_bib0004","series-title":"Canine and Feline Endocrinology","first-page":"378","article-title":"Canine hyperadrenocorticism","author":"Behrend","year":"2015"},{"issue":"n.5","key":"10.1016\/j.tcam.2026.101106_bib0005","doi-asserted-by":"crossref","first-page":"555","DOI":"10.2460\/ajvr.68.5.555","article-title":"Effect of low doses of cosyntropin on serum cortisol concentrations in clinically normaldogs","volume":"v.68","author":"Martin","year":"2007","journal-title":"Am J Vet Res"},{"issue":"n.4","key":"10.1016\/j.tcam.2026.101106_bib0006","doi-asserted-by":"crossref","first-page":"528","DOI":"10.2460\/javma.229.4.528","article-title":"Intramuscular administration of a low dose of ACTH for ACTH stimulation testing in dogs","volume":"v.229","author":"Behrend","year":"2006","journal-title":"J Am Vet Med Assoc"},{"issue":"n.03","key":"10.1016\/j.tcam.2026.101106_bib0007","article-title":"Use of low dose of synthetic ACTH in the adrenal stimulation test for diagnosis and monitoring of canine hyperadrenocorticism: evaluation of diagnostic efficacy","volume":"v.37","author":"Jeric\u00f3","year":"2017","journal-title":"Pesqui Vet Bras"},{"key":"10.1016\/j.tcam.2026.101106_bib0008","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1111\/jvim.14528","article-title":"Comparison of 2 doses for ACTH stimulation testing in dogs suspected of or treated for hyperadrenocorticism","volume":"v.30","author":"Aldridge","year":"2016","journal-title":"J Vet Intern Med"},{"issue":"n.6","key":"10.1016\/j.tcam.2026.101106_bib0009","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1111\/jvim.15256","article-title":"Low-dose ACTH stimulation testing in dogs suspected of hypoadrenocorticism","volume":"v.32","author":"Botsford","year":"2018","journal-title":"J Vet Intern Med"},{"issue":"n. 3","key":"10.1016\/j.tcam.2026.101106_bib0010","first-page":"734","article-title":"Effect of intravenous or perivascular injection of synthetic adrenocorticotropic hormone on the results of ACTH stimulation testing in dogs","volume":"v. 31","author":"Johnson","year":"2017","journal-title":"J Vet Intern Med"},{"issue":"n.10","key":"10.1016\/j.tcam.2026.101106_bib0011","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.2460\/javma.1998.212.10.1569","article-title":"Comparison of serum cortisol concentrations in clinically normal dogs after administration of freshly reconstituted versus reconstituted and stored frozen cosyntropin","volume":"v.212","author":"Frank","year":"1998","journal-title":"J Am Vet Med Assoc"},{"key":"10.1016\/j.tcam.2026.101106_bib0012","series-title":"Canine And Feline Endocrinology","first-page":"485","article-title":"Hypoadrenocorticism","author":"Scott\u2013Moncrieff","year":"2015"},{"issue":"n.03","key":"10.1016\/j.tcam.2026.101106_bib0013","article-title":"Use of low dose of synthetic ACTH in the adrenal stimulation test for diagnosis and monitoring of canine hyperadrenocorticism: evaluation of diagnostic efficacy","volume":"v.37","author":"Jeric\u00f3","year":"2017","journal-title":"Pesqui Vet Bras"},{"issue":"n.4","key":"10.1016\/j.tcam.2026.101106_bib0014","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1111\/j.1939-1676.2008.0118.x","article-title":"Use of a low-dose ACTH stimulation test for diagnosis of hypoadrenocorticism in dogs","volume":"v.22","author":"Lathan","year":"2008","journal-title":"J Vet Intern Med"},{"issue":"n.10","key":"10.1016\/j.tcam.2026.101106_bib0015","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.2460\/javma.1999.214.10.1497","article-title":"Evaluation of a low-dose synthetic adrenocorticotropic hormone stimulation test in clinically normal dogs and dogs with naturally developing hyperadrenocorticism","volume":"v.214","author":"Kerl","year":"1999","journal-title":"J Am Vet Med Assoc"},{"key":"10.1016\/j.tcam.2026.101106_bib0016","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/jvim.14710","article-title":"Biological variability in serum cortisol concentration post-adrenocorticotropic hormone stimulation in healthy dogs","volume":"v.31","author":"Gal","year":"2017","journal-title":"J Vet Intern Med"},{"issue":"n. 2","key":"10.1016\/j.tcam.2026.101106_bib0017","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1677\/joe.0.1030219","article-title":"Evidence for episodic but not circadian activity in plasma concentrations of adrenocorticotrophin, cortisol and thyroxine in dogs","volume":"v.103","author":"Kemppainen","year":"1984","journal-title":"J Endocrinol"},{"issue":"n. 2\u20133","key":"10.1016\/j.tcam.2026.101106_bib0018","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.ejphar.2007.11.073","article-title":"The significance of glucocorticoid pulsatility","volume":"v.583","author":"Lightman","year":"2008","journal-title":"Eur J Pharmacol"},{"key":"10.1016\/j.tcam.2026.101106_bib0019","series-title":"Basic Method Validation","author":"Westgard","year":"2008"},{"issue":"n. 4","key":"10.1016\/j.tcam.2026.101106_bib0020","first-page":"541","article-title":"Current and emerging concepts in biological and analytical variation applied in veterinary clinical pathology","volume":"v. 49","author":"Flatland","year":"2020","journal-title":"Vet Clin Pathol"},{"issue":"n. 5","key":"10.1016\/j.tcam.2026.101106_bib0021","first-page":"799","article-title":"Validation study of canine serum cortisol measurement with the Immulite 2000 Xpi cortisol immunoassay","volume":"v. 33","author":"Korchia","year":"2021","journal-title":"J Vet Diagn Investig"},{"issue":"n.1","key":"10.1016\/j.tcam.2026.101106_bib0022","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1590\/0001-3765201520140210","article-title":"Volume-dependent hemodynamic effects of blood collection in canine donors \u2013 evaluation of 13\u202f% and 15\u202f% of total blood volume depletion","volume":"v. 87","author":"Ferreira","year":"2015","journal-title":"An Acad Bras Ci\u00eanc"},{"issue":"n.5","key":"10.1016\/j.tcam.2026.101106_bib0023","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1111\/jvim.13598","article-title":"Evaluation of iron deficiency using reticulocyte indices in dogs enrolled in a blood donor program","volume":"v. 29","author":"Foy","year":"2015","journal-title":"J Vet Intern Med"},{"issue":"n. 4","key":"10.1016\/j.tcam.2026.101106_bib0024","doi-asserted-by":"crossref","first-page":"131","DOI":"10.5326\/JAAHA-MS-7444","article-title":"2024 AAHA fluid therapy guidelines for dogs and cats","volume":"v. 60","author":"Pardo","year":"2024","journal-title":"J Am Anim Hosp Assoc"},{"issue":"n. 2","key":"10.1016\/j.tcam.2026.101106_bib0025","doi-asserted-by":"crossref","first-page":"283","DOI":"10.2460\/ajvr.1988.49.02.283","article-title":"Evaluation of plasma aldosterone concentrations before and after ACTH administration in clinically normal dogs and in dogs with various diseases","volume":"v. 49","author":"Willard","year":"1988","journal-title":"Am J Vet Res"},{"issue":"n.6","key":"10.1016\/j.tcam.2026.101106_bib0026","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1172\/JCI108431","article-title":"The renin-angiotensin-aldosterone system in congestive failure in conscious dogs","volume":"v. 57","author":"Watkins","year":"1976","journal-title":"J Clin Investig"},{"issue":"n.2","key":"10.1016\/j.tcam.2026.101106_bib0027","first-page":"279","article-title":"Experimental aldosterone hypertension in the dog: effects on arterial pressure, blood volume and extracellular fluid volume","volume":"v. 4","author":"Krieger","year":"1982","journal-title":"Hypertension"},{"key":"10.1016\/j.tcam.2026.101106_bib0028","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/jvim.12243","article-title":"Evaluation of aldosterone concentrations in dogs with hypoadrenocorticism","volume":"v.28","author":"Baumstark","year":"2014","journal-title":"J Vet Intern Med"},{"key":"10.1016\/j.tcam.2026.101106_bib0029","series-title":"Fundamentals of Veterinary Clinical Pathology","first-page":"p.516","author":"Stockham","year":"2002"},{"key":"10.1016\/j.tcam.2026.101106_bib0030","first-page":"18","article-title":"A screening evaluation for endogenous glucocorticoid deficiency in dogs: a modified Thorn test","volume":"v. 25","author":"Chastain","year":"1989","journal-title":"J Am Anim Hosp Assoc"},{"issue":"n.1","key":"10.1016\/j.tcam.2026.101106_bib0031","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1751-0147-51-33","article-title":"Cortisol secretion after adrenocorticotrophin (ACTH) and dexamethasone tests in healthy female and male dogs","volume":"v. 51","author":"Pessina","year":"2009","journal-title":"Acta Vet Scand"},{"issue":"n.11","key":"10.1016\/j.tcam.2026.101106_bib0032","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1161\/CIRCRESAHA.112.277962","article-title":"Acute psychological stress accelerates reverse cholesterol transport via corticosterone-dependent inhibition of intestinal cholesterol absorption","volume":"v. 111","author":"Silvennoinen","year":"2012","journal-title":"Circ Res"},{"issue":"n.4","key":"10.1016\/j.tcam.2026.101106_bib0033","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1210\/endo-74-4-543","article-title":"Role of Cholesterol in the in Vivo biosynthesis of adrenal steroids by the dog","volume":"v. 74","author":"Krum","year":"1964","journal-title":"Endocrinology"},{"issue":"n.\u202f1","key":"10.1016\/j.tcam.2026.101106_bib0034","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.tvjl.2008.10.011","article-title":"Lipid metabolism and hyperlipidemia in dogs","volume":"v. 183","author":"Xenoulis","year":"2010","journal-title":"Vet J"},{"issue":"n.3","key":"10.1016\/j.tcam.2026.101106_bib0035","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1939-165X.1994.tb00683.x","article-title":"The influence of fasting on blood glucose, triglycerides, cholesterol, and alkaline phosphatase in rats","volume":"v. 23","author":"Waner","year":"1994","journal-title":"Vet Clin Pathol"},{"issue":"n.8","key":"10.1016\/j.tcam.2026.101106_bib0036","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.2460\/ajvr.2002.63.1083","article-title":"Alkaline phosphatase expression in tissues from glucocorticoid-treated dogs","volume":"v. 63","author":"Wiedemeyer","year":"2002","journal-title":"Am J Vet Res"},{"issue":"n. 11","key":"10.1016\/j.tcam.2026.101106_bib0037","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1590\/1678-5150-pvb-6105","article-title":"Epidemiological, clinical, and laboratory aspects in a case series of canine hyperadrenocorticism: 115 cases (2010-2014)","volume":"v. 39","author":"Martins","year":"2019","journal-title":"Pesqui Vet Bras"},{"issue":"n.11","key":"10.1016\/j.tcam.2026.101106_bib0038","doi-asserted-by":"crossref","first-page":"729","DOI":"10.2337\/diab.14.11.729","article-title":"Effect of adrenal steroids on glucose kinetics in normal and diabetic dogs","volume":"v. 14","author":"Ninomiya","year":"1965","journal-title":"Diabetes"},{"issue":"n.1","key":"10.1016\/j.tcam.2026.101106_bib0039","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1172\/JCI109264","article-title":"Synergistic interactions of physiologic increments of glucagon, epinephrine, and cortisol in the dog: a model for stress-induced hyperglycemia","volume":"v. 63","author":"Eigler","year":"1979","journal-title":"J Clin Invest"},{"key":"10.1016\/j.tcam.2026.101106_bib0040","series-title":"Fundamentals of Veterinary Clinical Pathology","first-page":"516","author":"Stockham","year":"2002"},{"issue":"n.2","key":"10.1016\/j.tcam.2026.101106_bib0041","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0026-0495(83)90221-4","article-title":"Importance of increased urinary calcium excretion in the development of secondary hyperparathyroidism of patients umder glucocorticoid therapy","volume":"v. 32","author":"Suzuki","year":"1983","journal-title":"Metabolism"},{"issue":"n. 11","key":"10.1016\/j.tcam.2026.101106_bib0042","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/j.1748-5827.2005.tb00282.x","article-title":"Hyperparathyroidism in dogs with hyperadrenocorticism","volume":"v. 46","author":"Ramsey","year":"2005","journal-title":"J Small Anim Pract"},{"issue":"n.5","key":"10.1016\/j.tcam.2026.101106_bib0043","first-page":"2429","article-title":"Loss of bone minerals and strength in rats with aldosteronism","volume":"v. 287","author":"Chhokar","year":"2004","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"n.1","key":"10.1016\/j.tcam.2026.101106_bib0044","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1111\/j.1523-1755.2004.00725.x","article-title":"Aldosterone increases renal calcium reabsorption through two types of channels","volume":"v. 66","author":"Leclerc","year":"2004","journal-title":"Kidney Int"}],"container-title":["Topics in Companion Animal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1938973626000620?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1938973626000620?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:16:14Z","timestamp":1792145774000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1938973626000620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"alternative-id":["S1938973626000620"],"URL":"https:\/\/doi.org\/10.1016\/j.tcam.2026.101106","relation":{},"ISSN":["1938-9736"],"issn-type":[{"value":"1938-9736","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biological response stability of refrigerated cosacthen after opening and non-hormonal effects of ACTH stimulation testing in healthy dogs","name":"articletitle","label":"Article Title"},{"value":"Topics in Companion Animal Medicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tcam.2026.101106","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"101106"}}