{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:43:44Z","timestamp":1790433824752,"version":"4.1.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Seneca Foundation of Murcia","award":["GERM 04543\/07"],"award-info":[{"award-number":["GERM 04543\/07"]}]},{"DOI":"10.13039\/501100004837","name":"MICINN","doi-asserted-by":"crossref","award":["AGL2009-12091"],"award-info":[{"award-number":["AGL2009-12091"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Seneca Foundation of Murcia","award":["07875\/BPS\/07"],"award-info":[{"award-number":["07875\/BPS\/07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Theriogenology"],"published-print":{"date-parts":[[2011,12]]},"DOI":"10.1016\/j.theriogenology.2011.06.032","type":"journal-article","created":{"date-parts":[[2011,8,28]],"date-time":"2011-08-28T20:26:10Z","timestamp":1314563170000},"page":"1667-1675","source":"Crossref","is-referenced-by-count":13,"title":["Effects of Hoechst 33342 staining and ultraviolet irradiation on the developmental competence of in vitro-matured porcine oocytes"],"prefix":"10.1016","volume":"76","author":[{"given":"C.","family":"Maside","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Gil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cuello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sanchez-Osorio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Parrilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Lucas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.N.","family":"Caama\u00f1o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Vazquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Roca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.A.","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.theriogenology.2011.06.032_bib1","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1002\/dvdy.20915","article-title":"Mammalian nuclear transfer","volume":"235","author":"Meissner","year":"2006","journal-title":"Dev Dyn"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib2","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.theriogenology.2007.05.059","article-title":"Somatic cell nuclear transfer: Past, present and future perspectives","volume":"68","author":"Campbell","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib3","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1038\/80242","article-title":"Production of cloned pigs from in vitro systems","volume":"18","author":"Betthauser","year":"2000","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib4","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1126\/science.289.5482.1188","article-title":"Pig cloning by microinjection of fetal fibroblast nuclei","volume":"289","author":"Onishi","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib5","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1038\/35024082","article-title":"Cloned pigs produced by nuclear transfer from adult somatic cells","volume":"407","author":"Polejaeva","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib6","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1095\/biolreprod65.5.1558","article-title":"Feasibility of producing porcine nuclear transfer embryos by using G2\/M-stage fetal fibroblasts as donors","volume":"65","author":"Lai","year":"2001","journal-title":"Biol Reprod"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib7","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1095\/biolreprod.102.014886","article-title":"Production of nuclear transfer-derived piglets using porcine fetal fibroblasts transfected with the enhanced green fluorescent protein","volume":"69","author":"Hyun","year":"2003","journal-title":"Biol Reprod"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib8","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1095\/biolreprod.103.015917","article-title":"Production of cloned pigs by whole-cell intracytoplasmic microinjection","volume":"69","author":"Lee","year":"2003","journal-title":"Biol Reprod"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib9","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1089\/clo.2008.0005","article-title":"Successful cloning of the Yucatan minipig using commercial\/occidental breeds as oocyte donors and embryo recipients","volume":"10","author":"Estrada","year":"2008","journal-title":"Cloning Stem Cells"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib10","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1262\/jrd.19110","article-title":"Effects of recloning on the efficiency of production of alpha 1,3-galactosyltransferase knockout pigs","volume":"54","author":"Fujimura","year":"2008","journal-title":"J Reprod Dev"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib11","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/10495390701839264","article-title":"A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methods","volume":"19","author":"Lee","year":"2008","journal-title":"Anim Biotechnol"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib12","first-page":"773","article-title":"Influence of ovulation status, seasonality and embryo transfer method on development of cloned porcine embryos","volume":"45","author":"Koo","year":"2010","journal-title":"Reprod Dom Anim"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib13","doi-asserted-by":"crossref","first-page":"10897","DOI":"10.1021\/bi980818l","article-title":"2'-Deoxyisoguanosine adopts more than one tautomer to form base pairs with thymidine observed by high-resolution crystal structure analysis","volume":"37","author":"Robinson","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib14","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1089\/15362300460743781","article-title":"Review of enucleation methods and procedures used in animal cloning: state of the art","volume":"6","author":"Li","year":"2004","journal-title":"Cloning Stem Cells"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib15","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/mrd.1080270206","article-title":"Potential of hypertonic medium treatment for embryo micromanipulation: II","volume":"27","author":"Yang","year":"1990","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib16","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1530\/jrf.0.0950475","article-title":"Viable embryos and normal calves after nuclear transfer into Hoechst stained enucleated demi-oocytes of cows","volume":"95","author":"Westhusin","year":"1992","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib17","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1530\/jrf.0.0990039","article-title":"Membrane and intracellular effects of ultraviolet irradiation with Hoechst 33342 on bovine secondary oocytes matured in vitro","volume":"99","author":"Smith","year":"1993","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib18","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1017\/S0967199402002265","article-title":"Effect of Hoechst 33342 staining on developmental competence of prepubertal goat oocytes","volume":"10","author":"Velilla","year":"2002","journal-title":"Zygote"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib19","doi-asserted-by":"crossref","first-page":"I170","DOI":"10.1093\/humrep\/de.25.s1.140","article-title":"Toxic effects of Hoechst staining and UV-irradiation on preimplantation development of parthenogenetically activated mouse oocytes","volume":"25","author":"Versieren","year":"2010","journal-title":"Hum Reprod"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib20","first-page":"61","article-title":"Culture of pig embryo","volume":"48","author":"Petters","year":"1993","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib21","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0093-691X(97)00270-7","article-title":"In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium","volume":"48","author":"Abeydeera","year":"1997","journal-title":"Theriogenology"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib22","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S0093-691X(03)00051-7","article-title":"Effect of the volume of medium and number of oocytes during in vitro fertilization on embryo development in pigs","volume":"60","author":"Gil","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib23","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/j.1939-4640.2004.tb02805.x","article-title":"Effects of centrifugation before freezing on boar sperm cryosurvival","volume":"25","author":"Carvajal","year":"2004","journal-title":"J Androl"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib24","series-title":"Probability Theory and Mathematical Statistics","first-page":"129","article-title":"Some Probability Distributions","author":"Fisz","year":"1980"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib25","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1017\/S0967199400000848","article-title":"Optimisation of porcine oocyte activation following nuclear transfer","volume":"8","author":"Tao","year":"2000","journal-title":"Zygote"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib26","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1530\/jrf.0.0820173","article-title":"Differential sensitivity of mouse pronuclei and zygote cytoplasm to Hoechst staining and ultraviolet irradiation","volume":"82","author":"Tsunoda","year":"1988","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib27","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1095\/biolreprod62.1.150","article-title":"Dynamic imaging of the metaphase II spindle and maternal chromosomesin bovine oocytes: implications for enucleation efficiency verification, avoidance of parthenogenesis, and successful embryogenesis","volume":"62","author":"Dominko","year":"2000","journal-title":"Biol Reprod"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib28","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1530\/jrf.0.1160363","article-title":"UV irradiation of pig metaphase chromosomes: maturation-promoting factor degradation, nuclear cytology and cell cycle progression","volume":"116","author":"Leal","year":"1999","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib29","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1002\/mrd.1080410413","article-title":"UV irradiation of chromosomal DNA and its effect upon MPF and meiosis in mammalian oocytes","volume":"41","author":"Bradshaw","year":"1995","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib30","doi-asserted-by":"crossref","first-page":"14289","DOI":"10.1073\/pnas.0706687104","article-title":"Effects of light on development of mammalian zygotes","volume":"104","author":"Takenaka","year":"2007","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib31","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.theriogenology.2008.09.023","article-title":"Hoechst 33342: the dye that enabled differentiation of living X-and Y-chromosome bearing mammalian sperm","volume":"71","author":"Garner","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib32","doi-asserted-by":"crossref","DOI":"10.1007\/s11010-011-0764-y","article-title":"Hoechst 33342 induced reactive oxygen species and impaired expression of cytochrome c oxidase subunit 1 leading to cell death in irradiated human cancer cells","author":"Athar","year":"2011","journal-title":"Mol Cell Biochem"},{"key":"10.1016\/j.theriogenology.2011.06.032_bib33","doi-asserted-by":"crossref","first-page":"936","DOI":"10.3109\/10715762.2010.492829","article-title":"Hoechst 33342 induces radiosensitization in malignant glioma cells via increase in mitochondrial reactive oxygen species","volume":"44","author":"Athar","year":"2010","journal-title":"Free Radic Res"}],"container-title":["Theriogenology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0093691X11003177?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0093691X11003177?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T12:58:03Z","timestamp":1543582683000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0093691X11003177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["S0093691X11003177"],"URL":"https:\/\/doi.org\/10.1016\/j.theriogenology.2011.06.032","relation":{},"ISSN":["0093-691X"],"issn-type":[{"value":"0093-691X","type":"print"}],"subject":[],"published":{"date-parts":[[2011,12]]}}}