{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:05:52Z","timestamp":1783109152025,"version":"3.54.6"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["CSC 201906220218"],"award-info":[{"award-number":["CSC 201906220218"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012456","name":"National Social Science Fund of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007601","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"EU Framework Programme for Research and Innovation Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["813409"],"award-info":[{"award-number":["813409"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.com.au","thrombosisresearch.com","clinicalkey.es","clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Thrombosis Research"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.thromres.2024.109208","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T09:02:28Z","timestamp":1730970148000},"page":"109208","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Functionally distinct anticoagulant mechanisms of endothelial cells"],"prefix":"10.1016","volume":"244","author":[{"given":"Claudia","family":"Sch\u00f6nichen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harmen","family":"Middelveld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Huskens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip G.","family":"de Groot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan W.M.","family":"Heemskerk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Roest","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bas","family":"de Laat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.thromres.2024.109208_bb0005","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1152\/physrev.00016.2011","article-title":"New fundamentals in hemostasis","volume":"93","author":"Versteeg","year":"2013","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.thromres.2024.109208_bb0010","doi-asserted-by":"crossref","first-page":"3568","DOI":"10.1182\/blood.V90.9.3568","article-title":"Association of tissue factor pathway inhibitor with human umbilical vein endothelial cells","volume":"90","author":"Hansen","year":"1997","journal-title":"Blood"},{"key":"10.1016\/j.thromres.2024.109208_bb0015","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1182\/blood-2009-10-250910","article-title":"Endothelial-derived tissue factor pathway inhibitor regulates arterial thrombosis but is not required for development or hemostasis","volume":"116","author":"White","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.thromres.2024.109208_bb0020","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00281-011-0285-5","article-title":"Endothelium: role in regulation of coagulation and inflammation","volume":"34","author":"Van Hinsbergh","year":"2012","journal-title":"Semin. Immunopathol."},{"key":"10.1016\/j.thromres.2024.109208_bb0025","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1055\/s-0035-1556586","article-title":"Homeostasis of hemostasis: the role of endothelium","volume":"41","author":"Kazmi","year":"2015","journal-title":"Semin. Thromb. Hemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0030","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.3324\/haematol.2018.208363","article-title":"Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice","volume":"104","author":"Wichaiyo","year":"2019","journal-title":"Haematologica"},{"key":"10.1016\/j.thromres.2024.109208_bb0035","doi-asserted-by":"crossref","DOI":"10.1063\/5.0023312","article-title":"Scalable manufacture of a disposable, storage-stable eight-channel microfluidic device for rapid testing of platelet, coagulation, and drug function under whole blood flow","volume":"14","author":"Rossi","year":"2020","journal-title":"Biomicrofluidics"},{"key":"10.1016\/j.thromres.2024.109208_bb0040","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/s41467-018-02990-x","article-title":"A microengineered vascularized bleeding model that integrates the principal components of hemostasis","volume":"9","author":"Sakurai","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.thromres.2024.109208_bb0045","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1161\/01.RES.0000255691.76142.4a","article-title":"Phenotypic heterogeneity of the endothelium: structure, function, and mechanisms","volume":"100","author":"Aird","year":"2007","journal-title":"Circ. Res."},{"key":"10.1016\/j.thromres.2024.109208_bb0050","doi-asserted-by":"crossref","first-page":"4411","DOI":"10.3390\/ijms20184411","article-title":"Vascular endothelial cell biology: an update","volume":"20","author":"Kr\u00fcger-Genge","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.thromres.2024.109208_bb0055","doi-asserted-by":"crossref","first-page":"2338","DOI":"10.3390\/cells10092338","article-title":"Endothelial heterogeneity in development and wound healing","volume":"10","author":"Gurevich","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.thromres.2024.109208_bb0060","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1203\/00006450-199912000-00001","article-title":"Comparison of tissue factor pathway in human umbilical vein and adult saphenous vein endothelial cells: implications for newborn hemostasis and for laboratory models of endothelial cell function","volume":"46","author":"Grabowski","year":"1999","journal-title":"Pediatr. Res."},{"key":"10.1016\/j.thromres.2024.109208_bb0065","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1111\/jth.14719","article-title":"Localized endothelial-based control of platelet aggregation and coagulation under flow: proof-of-principle vessel-on-a-chip study","volume":"18","author":"Brouns","year":"2020","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0070","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202300360RR","article-title":"Endothelial-mediated regulation of platelet activation: involvement of multiple protein kinases","volume":"38","author":"Provenzale","year":"2024","journal-title":"FASEB J."},{"key":"10.1016\/j.thromres.2024.109208_bb0075","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1172\/JCI117202","article-title":"Endothelial cells stimulated with tumor necrosis factor-alpha express varying amounts of tissue factor resulting in inhomogenous fibrin deposition in a native blood flow system. Effects of thrombin inhibitors","volume":"93","author":"Kirchhofer","year":"1994","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.thromres.2024.109208_bb0080","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1093\/ajcp\/78.3.424a","article-title":"Polybrene-neutralization of heparin","volume":"78","author":"Hoffmann","year":"1982","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.thromres.2024.109208_bb0085","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1160\/TH13-06-0493","article-title":"Additive roles of platelets and fibrinogen in whole-blood fibrin clot formation upon dilution as assessed by thromboelastometry","volume":"111","author":"Ninivaggi","year":"2014","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0090","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.bja.2018.01.023","article-title":"Anticoagulant and side-effects of protamine in cardiac surgery: a narrative review","volume":"120","author":"Boer","year":"2018","journal-title":"Br. J. Anaesth."},{"key":"10.1016\/j.thromres.2024.109208_bb0095","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1182\/blood-2009-05-222109","article-title":"Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation","volume":"114","author":"Ni Ainle","year":"2009","journal-title":"Blood"},{"key":"10.1016\/j.thromres.2024.109208_bb0100","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1177\/1060028020943160","article-title":"Andexanet alfa, the possible alternative to protamine for reversal of unfractionated heparin","volume":"55","author":"Maneno","year":"2021","journal-title":"Ann. Pharmacother."},{"key":"10.1016\/j.thromres.2024.109208_bb0105","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1007\/s40265-018-0940-4","article-title":"Andexanet alfa: first global approval","volume":"78","author":"Heo","year":"2018","journal-title":"Drugs"},{"key":"10.1016\/j.thromres.2024.109208_bb0110","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1056\/NEJMoa1814051","article-title":"Full study report of andexanet alfa for bleeding associated with factor Xa inhibitors","volume":"380","author":"Connolly","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.thromres.2024.109208_bb0115","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/j.bja.2024.02.027","article-title":"Antithrombin supplementation attenuates heparin resistance in plasma spiked with Gla-domainless factor Xa S195A in vitro","volume":"132","author":"Mishima","year":"2024","journal-title":"Br. J. Anaesth."},{"key":"10.1016\/j.thromres.2024.109208_bb0120","doi-asserted-by":"crossref","DOI":"10.1177\/1076029619863493","article-title":"Reversal of factor Xa inhibitors by andexanet alfa may increase thrombogenesis compared to pretreatment values","volume":"25","author":"Siddiqui","year":"2019","journal-title":"Clin. Appl. Thromb. Hemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0125","doi-asserted-by":"crossref","DOI":"10.1177\/10760296241247558","article-title":"Andexanet alfa neutralizes the anticoagulant effects of unfractionated heparin of bovine, ovine and porcine origin almost as protamine sulfate","volume":"30","author":"Siddiqui","year":"2024","journal-title":"Clin. Appl. Thromb. Hemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0130","doi-asserted-by":"crossref","first-page":"11910","DOI":"10.1038\/s41598-020-68438-9","article-title":"Platelet-primed interactions of coagulation and anticoagulation pathways in flow-dependent thrombus formation","volume":"10","author":"Brouns","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.thromres.2024.109208_bb0135","doi-asserted-by":"crossref","first-page":"6717","DOI":"10.1182\/bloodadvances.2023010027","article-title":"Crucial roles of red blood cells and platelets in whole blood thrombin generation","volume":"7","author":"Sun","year":"2023","journal-title":"Blood Adv."},{"key":"10.1016\/j.thromres.2024.109208_bb0140","doi-asserted-by":"crossref","DOI":"10.3390\/mi10110787","article-title":"Whole blood based multiparameter assessment of thrombus formation in a standard microfluidic device to proxy in vivo haemostasis and thrombosis","volume":"10","author":"Provenzale","year":"2019","journal-title":"Micromachines"},{"key":"10.1016\/j.thromres.2024.109208_bb0145","series-title":"Platelets in Thrombotic and Non-Thrombotic Disorders","first-page":"447","article-title":"Platelets and coagulation","author":"Heemskerk","year":"2017"},{"key":"10.1016\/j.thromres.2024.109208_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.blre.2020.100733","article-title":"Interplay between platelets and coagulation","volume":"46","author":"Sang","year":"2021","journal-title":"Blood Rev."},{"key":"10.1016\/j.thromres.2024.109208_bb0155","doi-asserted-by":"crossref","first-page":"12358","DOI":"10.1038\/s41598-021-91661-x","article-title":"Assessment of a complete and classified platelet proteome from genome-wide transcripts of human platelets and megakaryocytes covering platelet functions","volume":"11","author":"Huang","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.thromres.2024.109208_bb0160","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1160\/TH12-04-0269","article-title":"Coagulation on endothelial cells: the underexposed part of Virchow\u2019s triad","volume":"108","author":"Geenen","year":"2012","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0165","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.3390\/biomedicines11061669","article-title":"Development of personalized thrombogenesis and thrombin generation assays to assess endothelial dysfunction in cardiovascular diseases","volume":"11","author":"Bacci","year":"2023","journal-title":"Biomedicines"},{"key":"10.1016\/j.thromres.2024.109208_bb0170","doi-asserted-by":"crossref","first-page":"2810","DOI":"10.1111\/j.1538-7836.2010.04088.x","article-title":"Internalization of microparticles by endothelial cells promotes platelet\/endothelial cell interaction under flow","volume":"8","author":"Terrisse","year":"2010","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.thromres.2024.109208_bb0175","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1161\/CIRCRESAHA.108.187831","article-title":"The glycocalyx of the human umbilical vein endothelial cell: an impressive structure ex vivo but not in culture","volume":"104","author":"Chappell","year":"2009","journal-title":"Circ. Res."},{"key":"10.1016\/j.thromres.2024.109208_bb0180","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1016\/j.jacc.2011.11.057","article-title":"Heparanase regulates thrombosis in vascular injury and stent-induced flow disturbance","volume":"59","author":"Baker","year":"2012","journal-title":"J. Am. Coll. Cardiol."},{"key":"10.1016\/j.thromres.2024.109208_bb0185","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0167576","article-title":"Glycocalyx degradation induces a proinflammatory phenotype and increased leukocyte adhesion in cultured endothelial cells under flow","volume":"11","author":"McDonald","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.thromres.2024.109208_bb0190","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0186116","article-title":"Regeneration of glycocalyx by heparan sulfate and sphingosine 1-phosphate restores inter-endothelial communication","volume":"12","author":"Mensah","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.thromres.2024.109208_bb0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2023.120779","article-title":"Absolute pharmacokinetics of heparin in primates","volume":"311","author":"Song","year":"2023","journal-title":"Carbohydr. Polym."}],"container-title":["Thrombosis Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0049384824003402?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0049384824003402?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T14:43:04Z","timestamp":1737297784000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0049384824003402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":39,"alternative-id":["S0049384824003402"],"URL":"https:\/\/doi.org\/10.1016\/j.thromres.2024.109208","relation":{},"ISSN":["0049-3848"],"issn-type":[{"value":"0049-3848","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Functionally distinct anticoagulant mechanisms of endothelial cells","name":"articletitle","label":"Article Title"},{"value":"Thrombosis Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.thromres.2024.109208","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"109208"}}