{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T21:05:47Z","timestamp":1787951147872,"version":"build-2784847793"},"reference-count":76,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32322047"],"award-info":[{"award-number":["32322047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515030121"],"award-info":[{"award-number":["2024A1515030121"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515012643"],"award-info":[{"award-number":["2023A1515012643"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008868","name":"Jiangsu Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["BM2023009"],"award-info":[{"award-number":["BM2023009"]}],"id":[{"id":"10.13039\/501100008868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008868","name":"Jiangsu Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["CN2025101231482"],"award-info":[{"award-number":["CN2025101231482"]}],"id":[{"id":"10.13039\/501100008868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0901000"],"award-info":[{"award-number":["2018YFA0901000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0900100"],"award-info":[{"award-number":["2018YFA0900100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2104800"],"award-info":[{"award-number":["2022YFC2104800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Trends in Biotechnology"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.tibtech.2025.02.019","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T08:41:05Z","timestamp":1743324065000},"page":"1425-1445","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Expediting genome synthesis of Corynebacterium glutamicum with an artificial chromosome vector"],"prefix":"10.1016","volume":"43","author":[{"given":"Zhanhua","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peixiong","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zebin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baitao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8501-1408","authenticated-orcid":false,"given":"Yanrui","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5872-3269","authenticated-orcid":false,"given":"Yun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5223-7013","authenticated-orcid":false,"given":"Zhanglin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tibtech.2025.02.019_bb0005","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/nature10403","article-title":"Synthetic chromosome arms function in yeast and generate phenotypic diversity by design","volume":"477","author":"Dymond","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0010","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1126\/science.aaf4557","article-title":"Design of a synthetic yeast genome","volume":"355","author":"Richardson","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0015","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature20124","article-title":"Defining synonymous codon compression schemes by genome recoding","volume":"539","author":"Wang","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0020","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/s41586-019-1192-5","article-title":"Total synthesis of Escherichia coli with a recoded genome","volume":"569","author":"Fredens","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0025","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1101\/gr.193433.115","article-title":"SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes","volume":"26","author":"Shen","year":"2016","journal-title":"Genome Res."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0030","doi-asserted-by":"crossref","DOI":"10.1093\/nsr\/nwad073","article-title":"Dynamics of synthetic yeast chromosome evolution shaped by hierarchical chromatin organization","volume":"10","author":"Zhou","year":"2023","journal-title":"Natl. Sci. Rev."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0035","doi-asserted-by":"crossref","DOI":"10.1126\/science.aad6253","article-title":"Design and synthesis of a minimal bacterial genome","volume":"351","author":"Hutchison","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0040","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1126\/science.1151721","article-title":"Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome","volume":"319","author":"Gibson","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0045","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1126\/science.1190719","article-title":"Creation of a bacterial cell controlled by a chemically synthesized genome","volume":"329","author":"Gibson","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0050","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.aaf3639","article-title":"Design, synthesis, and testing toward a 57-codon genome","volume":"353","author":"Ostrov","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0055","article-title":"Synthetic genomes unveil the effects of synonymous recoding","author":"Nyerges","year":"2024","journal-title":"bioRxiv"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0060","doi-asserted-by":"crossref","first-page":"5220","DOI":"10.1016\/j.cell.2023.09.025","article-title":"Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions","volume":"186","author":"Zhao","year":"2023","journal-title":"Cell"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0065","doi-asserted-by":"crossref","first-page":"5237","DOI":"10.1016\/j.cell.2023.10.015","article-title":"Design, construction, and functional characterization of a tRNA neochromosome in yeast","volume":"186","author":"Schindler","year":"2023","journal-title":"Cell"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0070","article-title":"Methodological advances enabled by the construction of a synthetic yeast genome","volume":"4","author":"Schindler","year":"2024","journal-title":"Cell Rep. Methods"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0075","doi-asserted-by":"crossref","first-page":"6971","DOI":"10.1093\/nar\/gkx415","article-title":"Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA","volume":"45","author":"Lau","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0080","doi-asserted-by":"crossref","first-page":"8070","DOI":"10.1073\/pnas.1818259116","article-title":"Chemical synthesis rewriting of a bacterial genome to achieve design flexibility and biological functionality","volume":"116","author":"Venetz","year":"2019","journal-title":"Proc. Natl Acad. Sci. U. S. A."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2024.108421","article-title":"Advances on transfer and maintenance of large DNA in bacteria, fungi, and mammalian cells","volume":"76","author":"Bai","year":"2024","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0090","article-title":"The design and engineering of synthetic genomes","author":"James","year":"2024","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0095","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/s41477-023-01595-7","article-title":"A designer synthetic chromosome fragment functions in moss","volume":"10","author":"Chen","year":"2024","journal-title":"Nat. Plants"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0100","article-title":"Towards synthetic diatoms: the Phaeodactylum tricornutum Pt-syn 1.0 project","volume":"35","author":"Pampuch","year":"2022","journal-title":"Curr. Opin. Green Sustain. Chem."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0105","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.72847","article-title":"Resurrecting essential amino acid biosynthesis in mammalian cells","volume":"11","author":"Trolle","year":"2022","journal-title":"eLife"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0110","doi-asserted-by":"crossref","first-page":"4482","DOI":"10.1038\/s41467-022-31927-8","article-title":"Multiplex base editing to convert TAG into TAA codons in the human genome","volume":"13","author":"Chen","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0115","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaf4791","article-title":"Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome","volume":"355","author":"Shen","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0120","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaf4704","article-title":"\u2018Perfect\u2019 designer chromosome V and behavior of a ring derivative","volume":"355","author":"Xie","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0125","doi-asserted-by":"crossref","first-page":"4424","DOI":"10.1016\/j.molcel.2023.10.015","article-title":"Manipulating the 3D organization of the largest synthetic yeast chromosome","volume":"83","author":"Zhang","year":"2023","journal-title":"Mol. Cell"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0130","doi-asserted-by":"crossref","first-page":"12522","DOI":"10.1093\/nar\/gkad1024","article-title":"Metagenomics harvested genus-specific single-stranded DNA-annealing proteins improve and expand recombineering in Pseudomonas species","volume":"51","author":"Asin-Garcia","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0135","doi-asserted-by":"crossref","first-page":"3237","DOI":"10.1021\/acssynbio.1c00355","article-title":"Synthetic biology toolkits and metabolic engineering applied in Corynebacterium glutamicum for biomanufacturing","volume":"10","author":"Chai","year":"2021","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0140","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1021\/acssynbio.1c00644","article-title":"Genomic iterative replacements of large synthetic DNA fragments in Corynebacterium glutamicum","volume":"11","author":"Ye","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0145","doi-asserted-by":"crossref","DOI":"10.1093\/genetics\/iyab038","article-title":"De novo assembly and delivery to mouse cells of a 101 kb functional human gene","volume":"218","author":"Mitchell","year":"2021","journal-title":"Genetics"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0150","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1016\/j.cell.2022.06.046","article-title":"Synthetic chromosomes, genomes, viruses, and cells","volume":"185","author":"Venter","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0155","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s00253-005-1976-4","article-title":"Multiple large segment deletion method for Corynebacterium glutamicum","volume":"69","author":"Suzuki","year":"2005","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0160","doi-asserted-by":"crossref","first-page":"3597","DOI":"10.1007\/s00253-020-10517-y","article-title":"Homing endonuclease I-SceI-mediated Corynebacterium glutamicum ATCC 13032 genome engineering","volume":"104","author":"Wu","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0165","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1038\/s41596-020-00464-3","article-title":"Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS","volume":"16","author":"Robertson","year":"2021","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0170","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0580-9517(02)33014-9","article-title":"Cloning the metagenome: culture-independent access to the diversity and functions of the uncultivated microbial world","volume":"33","author":"Handelsman","year":"2002","journal-title":"Methods Microbiol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0175","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1128\/AEM.00415-07","article-title":"Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp","volume":"74","author":"Hain","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0180","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/BF00338997","article-title":"Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2","volume":"181","author":"Stalker","year":"1981","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0185","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.plasmid.2010.06.004","article-title":"Replication and partitioning of the broad-host-range plasmid RK2","volume":"64","author":"Kolatka","year":"2010","journal-title":"Plasmid"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0190","series-title":"YAC Protocols","first-page":"85","article-title":"Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes","author":"Kouprina","year":"2006"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0195","doi-asserted-by":"crossref","first-page":"8794","DOI":"10.1073\/pnas.89.18.8794","article-title":"Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector","volume":"89","author":"Shizuya","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0200","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1021\/acssynbio.0c00008","article-title":"Overcoming the challenges of megabase-sized plasmid construction in Escherichia coli","volume":"9","author":"Mukai","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0205","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1574-6968.2004.tb09623.x","article-title":"Characterization of the replicon of a 51-kb native plasmid from the gram-positive bacterium Leifsonia xyli subsp. cynodontis","volume":"236","author":"Li","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0210","doi-asserted-by":"crossref","first-page":"8103","DOI":"10.1128\/JB.01232-06","article-title":"A Type Ib ParB protein involved in plasmid partitioning in a gram-positive bacterium","volume":"188","author":"Yin","year":"2006","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0215","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1574-6968.2000.tb09062.x","article-title":"A Brevibacterium lactofermentum 16S rRNA gene used as target site for homologous recombination","volume":"185","author":"Amador","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0220","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1134\/S1022795416100070","article-title":"Nucleotide sequence and structural analysis of cryptic plasmid pBL90 from Brevibacterium lactofermentum","volume":"52","author":"Novikov","year":"2016","journal-title":"Russ. J. Genet."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0225","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0378-1119(91)90365-I","article-title":"New pUC-derived cloning vectors with different selectable markers and DNA replication origins","volume":"100","author":"Jeffrey","year":"1991","journal-title":"Gene"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0230","doi-asserted-by":"crossref","DOI":"10.15252\/msb.20188516","article-title":"A conserved mechanism drives partition complex assembly on bacterial chromosomes and plasmids","volume":"14","author":"Debaugny","year":"2018","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0235","doi-asserted-by":"crossref","DOI":"10.1002\/mbo3.1344","article-title":"A new role for monomeric ParA\/Soj in chromosome dynamics in Bacillus subtilis","volume":"12","author":"Roberts","year":"2023","journal-title":"MicrobiologyOpen"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0240","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1038\/s41467-020-15238-4","article-title":"Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum","volume":"11","author":"B\u00f6hm","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0245","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1186\/s12934-017-0815-5","article-title":"Development of a CRISPR\/Cas9 genome editing toolbox for Corynebacterium glutamicum","volume":"16","author":"Liu","year":"2017","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0250","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ymben.2017.06.010","article-title":"CRISPR\/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum","volume":"42","author":"Cho","year":"2017","journal-title":"Metab. Eng."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0255","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s12934-018-0910-2","article-title":"A RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum","volume":"17","author":"Wang","year":"2018","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0260","doi-asserted-by":"crossref","first-page":"6006","DOI":"10.1128\/AEM.01634-13","article-title":"Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology","volume":"79","author":"Baumgart","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0265","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1101\/gad.14.15.1971","article-title":"RecE\/RecT and Redalpha\/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners","volume":"14","author":"Muyrers","year":"2000","journal-title":"Genes Dev."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0270","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.751334","article-title":"Construction of an IS-free Corynebacterium glutamicum ATCC13032 chassis strain and random mutagenesis using the endogenous ISCg1 transposase","volume":"9","author":"Linder","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0275","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.plasmid.2011.11.001","article-title":"Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum","volume":"67","author":"Tan","year":"2012","journal-title":"Plasmid"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0280","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1111\/1751-7915.13444","article-title":"An update of the suicide plasmid-mediated genome editing system in Corynebacterium glutamicum","volume":"12","author":"Wang","year":"2019","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0285","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1016\/j.csbj.2023.03.004","article-title":"Synthetic biology tools for engineering Corynebacterium glutamicum","volume":"21","author":"Kim","year":"2023","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0290","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/s41597-020-0484-9","article-title":"CoryneRegNet 7, the reference database and analysis platform for corynebacterial gene regulatory networks","volume":"7","author":"Parise","year":"2020","journal-title":"Sci. Data"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0295","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1007\/s00253-015-7165-1","article-title":"Identification of the phd gene cluster responsible for phenylpropanoid utilization in Corynebacterium glutamicum","volume":"100","author":"Kallscheuer","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0300","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1021\/acssynbio.7b00261","article-title":"Corynebacterium glutamicum chassis C1*: building and testing a novel platform host for synthetic biology and industrial biotechnology","volume":"7","author":"Baumgart","year":"2018","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0305","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1574-6968.2008.01425.x","article-title":"Myo-inositol facilitators IolT1 and IolT2 enhance d-mannitol formation from d-fructose in Corynebacterium glutamicum","volume":"290","author":"B\u00e4umchen","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0310","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymben.2021.12.013","article-title":"Modular, synthetic chromosomes as new tools for large scale engineering of metabolism","volume":"72","author":"Postma","year":"2022","journal-title":"Metab. Eng."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0315","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/s41422-024-00934-3","article-title":"Convenient synthesis and delivery of a megabase-scale designer accessory chromosome empower biosynthetic capacity","volume":"34","author":"Ma","year":"2024","journal-title":"Cell Res."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0320","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.ymben.2020.08.007","article-title":"Engineering Corynebacterium glutamicum with a comprehensive genomic library and phage-based vectors","volume":"62","author":"Marques","year":"2020","journal-title":"Metab. Eng."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0325","doi-asserted-by":"crossref","first-page":"3581","DOI":"10.1093\/nar\/gkac181","article-title":"Activating cryptic biosynthetic gene cluster through a CRISPR-Cas12a-mediated direct cloning approach","volume":"50","author":"Liang","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0330","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/s41586-023-06268-1","article-title":"Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly","volume":"619","author":"Z\u00fcrcher","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0335","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"Langmead","year":"2009","journal-title":"Genome Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0340","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1101\/gr.107524.110","article-title":"The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data","volume":"20","author":"McKenna","year":"2010","journal-title":"Genome Res."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0345","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0350","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1093\/bioinformatics\/btp120","article-title":"TopHat: discovering splice junctions with RNA-Seq","volume":"25","author":"Trapnell","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0355","doi-asserted-by":"crossref","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential expression analysis for sequence count data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0360","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0378-1119(94)90324-7","article-title":"Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum","volume":"145","author":"Sch\u00e4fer","year":"1994","journal-title":"Gene"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0365","doi-asserted-by":"crossref","DOI":"10.1016\/j.gene.2021.145693","article-title":"Efficient genome editing for Pseudomonas aeruginosa using CRISPR-Cas12a","volume":"790","author":"Lin","year":"2021","journal-title":"Gene"},{"key":"10.1016\/j.tibtech.2025.02.019_bb0370","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1128\/AEM.04023-14","article-title":"Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system","volume":"81","author":"Jiang","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0375","doi-asserted-by":"crossref","first-page":"5595","DOI":"10.1128\/jb.175.17.5595-5603.1993","article-title":"Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon","volume":"175","author":"Keilhauer","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.tibtech.2025.02.019_bb0380","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1101\/gr.092759.109","article-title":"Circos: an information aesthetic for comparative genomics","volume":"19","author":"Krzywinski","year":"2009","journal-title":"Genome Res."}],"container-title":["Trends in Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167779925000824?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167779925000824?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T11:53:07Z","timestamp":1778673187000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167779925000824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":76,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S0167779925000824"],"URL":"https:\/\/doi.org\/10.1016\/j.tibtech.2025.02.019","relation":{},"ISSN":["0167-7799"],"issn-type":[{"value":"0167-7799","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expediting genome synthesis of Corynebacterium glutamicum with an artificial chromosome vector","name":"articletitle","label":"Article Title"},{"value":"Trends in Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tibtech.2025.02.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}