{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:09:22Z","timestamp":1789384162848,"version":"build-2803163510"},"reference-count":36,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Tissue and Cell"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1016\/j.tice.2014.10.008","type":"journal-article","created":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T03:30:27Z","timestamp":1415331027000},"page":"39-48","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["Spheroid cultures promote the stemness of corneal stromal cells"],"prefix":"10.1016","volume":"47","author":[{"given":"Hongyang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianchang","family":"Shu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjie","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonglong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tice.2014.10.008_bib0005","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1097\/ICO.0b013e318213796b","article-title":"Comparison of stem cell properties in cell populations isolated from human central and limbal corneal epithelium","volume":"30","author":"Chang","year":"2011","journal-title":"Cornea"},{"key":"10.1016\/j.tice.2014.10.008_bib0010","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1111\/j.1525-1594.2007.00440.x","article-title":"Morphological characteristics and proliferation of keratocytes cultured under simulated microgravity","volume":"31","author":"Chen","year":"2007","journal-title":"Artif. Organs"},{"key":"10.1016\/j.tice.2014.10.008_bib0015","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1089\/ten.tea.2008.0251","article-title":"Formation of keratocyte spheroids on chitosan-coated surface can maintain keratocyte phenotypes","volume":"15","author":"Chen","year":"2009","journal-title":"Tissue Eng. Part A"},{"key":"10.1016\/j.tice.2014.10.008_bib0020","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/j.biomaterials.2011.11.049","article-title":"The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities","volume":"33","author":"Cheng","year":"2012","journal-title":"Biomaterials"},{"key":"10.1016\/j.tice.2014.10.008_bib0025","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1080\/14653240701218508","article-title":"Mesenchymal stromal cell-like characteristics of corneal keratocytes","volume":"9","author":"Choong","year":"2007","journal-title":"Cytotherapy"},{"key":"10.1016\/j.tice.2014.10.008_bib0030","doi-asserted-by":"crossref","first-page":"e50114","DOI":"10.1371\/journal.pone.0050114","article-title":"Characterizing the effects of VPA VC and RCCS on rabbit keratocytes onto decellularized bovine cornea","volume":"7","author":"Dai","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.tice.2014.10.008_bib0035","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1101\/gad.1061803","article-title":"In vitro propagation and transcriptional profiling of humanmammarystem\/progenitor cells","volume":"17","author":"Dontu","year":"2003","journal-title":"Genes Dev."},{"key":"10.1016\/j.tice.2014.10.008_bib0040","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1096\/fj.04-2770fje","article-title":"PAX6 expression identifies progenitor cells for corneal keratocytes","volume":"19","author":"Funderburgh","year":"2005","journal-title":"FASEB J."},{"key":"10.1016\/j.tice.2014.10.008_bib0045","first-page":"308","article-title":"Keratocyte phenotype is enhanced in the absence of attachment to the substratum","volume":"14","author":"Funderburgh","year":"2008","journal-title":"Mol. Vis."},{"key":"10.1016\/j.tice.2014.10.008_bib0050","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.exer.2010.06.021","article-title":"The molecular basis of corneal transparency","volume":"91","author":"Hassell","year":"2010","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.tice.2014.10.008_bib0055","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/nmeth.1403","article-title":"Non-genetic method for purifying stem cell derived cardiomyocytes","volume":"7","author":"Hattori","year":"2010","journal-title":"Nat. Methods"},{"key":"10.1016\/j.tice.2014.10.008_bib0060","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0014-4835(03)00188-X","article-title":"Modulation of cultured corneal keratocytephenotype by growth factors\/cytokines control in vitro contractility andextracellular matrix contraction","volume":"77","author":"Jester","year":"2003","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.tice.2014.10.008_bib0065","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1016\/j.transproceed.2013.11.074","article-title":"Artificial islets from hybrid spheroids of three pancreatic cell lines","volume":"46","author":"Jo","year":"2014","journal-title":"Transplant. Proc."},{"key":"10.1016\/j.tice.2014.10.008_bib0070","doi-asserted-by":"crossref","first-page":"4815","DOI":"10.1016\/j.biomaterials.2014.02.045","article-title":"Microchip-based engineering of super-pancreaticis lets supported by adipose-derived stem cells","volume":"35","author":"Jun","year":"2014","journal-title":"Biomaterials"},{"key":"10.1016\/j.tice.2014.10.008_bib0075","first-page":"1790","article-title":"Preparation of recombinant OCT4 with penetrating peptide PTD and activity assay [Article in Chinese]","volume":"27","author":"Li","year":"2011","journal-title":"Chin. J. Pathophysiol."},{"key":"10.1016\/j.tice.2014.10.008_bib0080","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1002\/biot.200700228","article-title":"Recent advances in three-dimensional multicellular spheroid culture for biomedical research","volume":"3","author":"Lin","year":"2008","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.tice.2014.10.008_bib0085","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1016\/j.biomaterials.2005.08.031","article-title":"Enhanced cell survival of melanocyte spheroids inserum starvation condition","volume":"27","author":"Lin","year":"2006","journal-title":"Biomaterials"},{"key":"10.1016\/j.tice.2014.10.008_bib0090","first-page":"23","article-title":"Preparation and activity identification of recombinantPTD-Sox2 [Article in Chinese]","volume":"19","author":"Liu","year":"2011","journal-title":"Biotechnology"},{"key":"10.1016\/j.tice.2014.10.008_bib0095","first-page":"197","article-title":"Isolation and distribution of rabbit keratocyte precursors","volume":"14","author":"Mimura","year":"2008","journal-title":"Mol. Vis."},{"key":"10.1016\/j.tice.2014.10.008_bib0100","first-page":"1819","article-title":"Tissue engineering of corneal stroma with rabbit fibroblast precursors and gelatin hydrogels","volume":"14","author":"Mimura","year":"2008","journal-title":"Mol. Vis."},{"key":"10.1016\/j.tice.2014.10.008_bib0105","doi-asserted-by":"crossref","first-page":"C1109","DOI":"10.1152\/ajpcell.1997.273.4.C1109","article-title":"Three-dimensional cell cultures: from molecularmechanisms to clinical applications","volume":"273","author":"Mueller-Klieser","year":"1997","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.tice.2014.10.008_bib0110","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00441-012-1441-5","article-title":"Three-dimensional tissue cultures: current trends and beyond","volume":"352","author":"Page","year":"2013","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.tice.2014.10.008_bib0115","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.stem.2011.04.007","article-title":"Eyes wide open: a critical review of spheroid-formation as an assay for stem cells","volume":"8","author":"Pastrana","year":"2011","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.tice.2014.10.008_bib0120","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1002\/stem.1100","article-title":"Concise review: stem cells in the corneal stroma","volume":"30","author":"Pinnamaneni","year":"2012","journal-title":"Stem Cells"},{"key":"10.1016\/j.tice.2014.10.008_bib0125","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1126\/science.1553558","article-title":"Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system","volume":"255","author":"Reynolds","year":"1992","journal-title":"Science"},{"key":"10.1016\/j.tice.2014.10.008_bib0130","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.preteyeres.2008.08.001","article-title":"Prelude to corneal tissue engineering gaining control of collagen organization","volume":"27","author":"Ruberti","year":"2008","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.tice.2014.10.008_bib0135","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1016\/j.exer.2011.10.004","article-title":"Spheroid formation from corneal keratocytes and phenotype specific markers","volume":"93","author":"Scott","year":"2011","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.tice.2014.10.008_bib0140","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1167\/iovs.03-0256","article-title":"Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes","volume":"45","author":"Stramer","year":"2004","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.tice.2014.10.008_bib0145","first-page":"48","article-title":"Preparation of recombinant PTD-KLF4 with penetrating peptide and activity Assay [Article in Chinese]","volume":"31","author":"Su","year":"2011","journal-title":"China Biotechnol."},{"key":"10.1016\/j.tice.2014.10.008_bib0150","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/cyto.a.20671","article-title":"Stem cells of the adult cornea: from cytometric markers to therapeutic applications","volume":"75","author":"Tak\u00e1cs","year":"2009","journal-title":"Cytometry A"},{"key":"10.1016\/j.tice.2014.10.008_bib0155","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1387\/ijdb.103193mt","article-title":"Exploring refined conditions for reprogramming cells by recombinant Oct4 protein","volume":"54","author":"Their","year":"2010","journal-title":"Int. J. Dev. Biol."},{"key":"10.1016\/j.tice.2014.10.008_bib0160","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1126\/science.287.5460.2032","article-title":"Retinal stem cells in the adult mammalianeye","volume":"287","author":"Tropepe","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.tice.2014.10.008_bib0165","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1167\/iovs.04-0288","article-title":"Spheroidformation and expression ofneural proteins byhuman corneal stromal cells in vitro","volume":"46","author":"Uchida","year":"2005","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.tice.2014.10.008_bib0170","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.ophtha.2006.07.042","article-title":"Distribution of precursors in human corneal stromal cells and endothelialcells","volume":"114","author":"Yamagami","year":"2007","journal-title":"Ophthalmology"},{"key":"10.1016\/j.tice.2014.10.008_bib0175","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1167\/iovs.04-1405","article-title":"Serum-free spheroid culture of mouse corneal keratocytes","volume":"46","author":"Yoshida","year":"2005","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.tice.2014.10.008_bib0180","first-page":"1739","article-title":"Preparation and identification of recombinant PTD-maxadilan [Article in Chinese]","volume":"25","author":"Zeng","year":"2009","journal-title":"Sheng Wu Gong Cheng Xue Bao"}],"container-title":["Tissue and Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816614001165?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816614001165?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T20:13:09Z","timestamp":1758571989000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0040816614001165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S0040816614001165"],"URL":"https:\/\/doi.org\/10.1016\/j.tice.2014.10.008","relation":{},"ISSN":["0040-8166"],"issn-type":[{"value":"0040-8166","type":"print"}],"subject":[],"published":{"date-parts":[[2015,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Spheroid cultures promote the stemness of corneal stromal cells","name":"articletitle","label":"Article Title"},{"value":"Tissue and Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tice.2014.10.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}