{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T10:43:43Z","timestamp":1773917023134,"version":"3.50.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7252026"],"award-info":[{"award-number":["7252026"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Tissue and Cell"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.tice.2025.103263","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T08:03:39Z","timestamp":1764921819000},"page":"103263","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["U0126 induces osteoclast differentiation via the p38-NFATc-1 signaling pathway"],"prefix":"10.1016","volume":"99","author":[{"given":"Jiran","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueting","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangyong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-5742","authenticated-orcid":false,"given":"Xiaofeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.tice.2025.103263_bib1","doi-asserted-by":"crossref","first-page":"12515","DOI":"10.1096\/fj.201802805RR","article-title":"Activation of ROS\/MAPKs\/NF-\u03baB\/NLRP3 and inhibition of efferocytosis in osteoclast-mediated diabetic osteoporosis","volume":"33","author":"An","year":"2019","journal-title":"FASEB J."},{"key":"10.1016\/j.tice.2025.103263_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2024.112906","article-title":"A natural agent, 5-deoxycajanin, mitigates estrogen-deficiency bone loss via modulating osteoclast-osteoblast homeostasis","volume":"141","author":"Chen","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.tice.2025.103263_bib3","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.7150\/ijbs.94863","article-title":"Fgf9 regulates bone marrow mesenchymal stem cell fate and bone-fat balance in osteoporosis by PI3K\/AKT\/Hippo and MEK\/ERK signaling","volume":"20","author":"Chen","year":"2024","journal-title":"Int. J. Biol. Sci."},{"key":"10.1016\/j.tice.2025.103263_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232113512","article-title":"ERK inhibition increases RANKL-induced osteoclast differentiation in RAW 264.7 cells by stimulating AMPK activation and RANK expression and inhibiting anti-osteoclastogenic factor expression","volume":"23","author":"Choi","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tice.2025.103263_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2024.1338951","article-title":"Pharmacological and mechanistic aspects of quercetin in osteoporosis","volume":"15","author":"Deng","year":"2024","journal-title":"Front. Pharm."},{"key":"10.1016\/j.tice.2025.103263_bib6","article-title":"Dihydroartemisinin attenuates osteoclast formation and bone resorption via inhibiting the NF\u2011\u03baB, MAPK and NFATc1 signaling pathways and alleviates osteoarthritis","volume":"49","author":"Ding","year":"2022","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.tice.2025.103263_bib7","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/3830212","article-title":"Molecular and cellular pathways contributing to joint damage in rheumatoid arthritis","volume":"2020","author":"Fang","year":"2020","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.tice.2025.103263_bib8","doi-asserted-by":"crossref","first-page":"6337","DOI":"10.2147\/IJN.S456545","article-title":"pH-responsive mesoporous silica nanoparticles loaded with naringin for targeted osteoclast inhibition and bone regeneration","volume":"19","author":"Gong","year":"2024","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.tice.2025.103263_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2024.107409","article-title":"Endogenous oncogenic KRAS expression increases cell proliferation and motility in near-diploid hTERT RPE-1 cells","volume":"300","author":"Hindul","year":"2024","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.tice.2025.103263_bib10","doi-asserted-by":"crossref","first-page":"47366","DOI":"10.1074\/jbc.M208284200","article-title":"U0126 and PD98059, specific inhibitors of MEK, accelerate differentiation of RAW264.7 cells into osteoclast-like cells","volume":"277","author":"Hotokezaka","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.tice.2025.103263_bib11","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jare.2023.09.001","article-title":"Irisin as an agent for protecting against osteoporosis: A review of the current mechanisms and pathways","volume":"62","author":"Hu","year":"2024","journal-title":"J. Adv. Res."},{"key":"10.1016\/j.tice.2025.103263_bib12","doi-asserted-by":"crossref","first-page":"4650","DOI":"10.1002\/ptr.8299","article-title":"Trifolirhizin reduces osteoclast formation and prevents inflammatory osteolysis by inhibiting RANKL-induced activation of NF-\u03baB and MAPK signaling pathways and ROS","volume":"38","author":"Huang","year":"2024","journal-title":"Phytother. Res."},{"key":"10.1016\/j.tice.2025.103263_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2023.105461","article-title":"Oxyfluorfen induces cell cycle arrest by regulating MAPK, PI3K and autophagy in ruminant immortalized mammary epithelial cells","volume":"193","author":"Jang","year":"2023","journal-title":"Pest. Biochem. Physiol."},{"key":"10.1016\/j.tice.2025.103263_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2022.115207","article-title":"Heat shock protein 90 inhibitor 17-AAG down-regulates thymidine phosphorylase expression and potentiates the cytotoxic effect of tamoxifen and erlotinib in human lung squamous carcinoma cells","volume":"204","author":"Ko","year":"2022","journal-title":"Biochem. Pharm."},{"key":"10.1016\/j.tice.2025.103263_bib15","doi-asserted-by":"crossref","first-page":"8792","DOI":"10.1038\/s41467-024-53119-2","article-title":"A RANKL-UCHL1-sCD13 negative feedback loop limits osteoclastogenesis in subchondral bone to prevent osteoarthritis progression","volume":"15","author":"Liang","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tice.2025.103263_bib16","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.3389\/fimmu.2018.02847","article-title":"Sonic hedgehog signaling pathway mediates proliferation and migration of fibroblast-like synoviocytes in rheumatoid arthritis via MAPK\/ERK signaling pathway","volume":"9","author":"Liu","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.tice.2025.103263_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.tice.2024.102643","article-title":"The Shh-p38-NFATc1 signaling pathway is essential for osteoclastogenesis during tooth eruption","volume":"92","author":"Liu","year":"2025","journal-title":"Tissue Cell"},{"key":"10.1016\/j.tice.2025.103263_bib18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s10020-024-00931-7","article-title":"Erythropoietin regulates osteoclast formation via up-regulating PPAR\u03b3 expression","volume":"30","author":"Liu","year":"2024","journal-title":"Mol. Med."},{"key":"10.1016\/j.tice.2025.103263_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2025.114377","article-title":"DPHB inhibits osteoclastogenesis by suppressing NF-\u03baB and MAPK signaling and alleviates inflammatory bone destruction","volume":"152","author":"Liu","year":"2025","journal-title":"Int Immunopharmacol."},{"key":"10.1016\/j.tice.2025.103263_bib20","doi-asserted-by":"crossref","DOI":"10.3390\/jdb8030012","article-title":"A synthetic peptide, CK2.3, inhibits RANKL-induced osteoclastogenesis through BMPRIa and ERK signaling pathway","volume":"8","author":"Nguyen","year":"2020","journal-title":"J. Dev. Biol."},{"key":"10.1016\/j.tice.2025.103263_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2024.1454058","article-title":"Role of hedgehog signaling in the pathogenesis and therapy of heterotopic ossification","volume":"12","author":"Pei","year":"2024","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.tice.2025.103263_bib22","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21238965","article-title":"Long-lasting activity of ERK kinase depends on NFATc1 induction and is involved in cell migration-fusion in murine macrophages RAW264.7","volume":"21","author":"Russo","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tice.2025.103263_bib23","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s12079-007-0010-2","article-title":"Specific inhibitor of MEK-mediated cross-talk between ERK and p38 MAPK during differentiation of human osteosarcoma cells","volume":"1","author":"Shimo","year":"2007","journal-title":"J. Cell Commun. Signal"},{"key":"10.1016\/j.tice.2025.103263_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2025.117424","article-title":"ERK inhibits osteoclast differentiation in RAW 264.7 cells through the osteoprotegerin-mediated autophagy","volume":"193","author":"Shin","year":"2025","journal-title":"Bone"},{"key":"10.1016\/j.tice.2025.103263_bib25","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.778078","article-title":"Macrophage-osteoclast associations: origin, polarization, and subgroups","volume":"12","author":"Sun","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.tice.2025.103263_bib26","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1096\/fj.201700672RR","article-title":"A ERK\/RSK-mediated negative feedback loop regulates M-CSF-evoked PI3K\/AKT activation in macrophages","volume":"32","author":"Wang","year":"2018","journal-title":"FASEB J."},{"key":"10.1016\/j.tice.2025.103263_bib27","article-title":"MCU inhibitor ruthenium red alleviates the osteoclastogenesis and ovariectomized osteoporosis via suppressing RANKL-induced ROS production and NFATc1 activation through P38 MAPK signaling pathway","volume":"2022","author":"Wang","year":"2022","journal-title":"Oxid. Med. Cell Longev."},{"key":"10.1016\/j.tice.2025.103263_bib28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s13020-024-00938-6","article-title":"Cirsilineol inhibits RANKL-induced osteoclast activity and ovariectomy-induced bone loss via NF-\u03bab\/ERK\/p38 signaling pathways","volume":"19","author":"Wang","year":"2024","journal-title":"Chin. Med."},{"key":"10.1016\/j.tice.2025.103263_bib29","doi-asserted-by":"crossref","first-page":"20383","DOI":"10.1021\/acs.jafc.4c02982","article-title":"Tetrahydroberberine prevents ovariectomy-induced bone loss by inhibiting RANKL-induced osteoclastogenesis and promoting osteoclast apoptosis","volume":"72","author":"Wu","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.tice.2025.103263_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2025.114630","article-title":"Therapeutic potential of Sweroside in postmenopausal osteoporosis: Inhibition of osteoclast differentiation and promotion of osteoclast apoptosis via NF-\u03baB and MAPK pathways","volume":"155","author":"Wu","year":"2025","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.tice.2025.103263_bib31","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s12964-023-01423-7","article-title":"IL13R\u03b12 as a crucial receptor for Chi3l1 in osteoclast differentiation and bone resorption through the MAPK\/AKT pathway","volume":"22","author":"Xu","year":"2024","journal-title":"Cell Commun. Signal"},{"key":"10.1016\/j.tice.2025.103263_bib32","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s00441-014-2025-3","article-title":"U0126 promotes osteogenesis of rat bone-marrow-derived mesenchymal stem cells by activating BMP\/Smad signaling pathway","volume":"359","author":"Xu","year":"2015","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.tice.2025.103263_bib33","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1177\/0022034520970471","article-title":"Nicotinamide improves delayed tooth eruption in Runx2+\/- mice","volume":"100","author":"Yoon","year":"2021","journal-title":"J. Dent. Res."},{"key":"10.1016\/j.tice.2025.103263_bib34","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.927083","article-title":"U0126: not only a MAPK kinase inhibitor","volume":"13","author":"You","year":"2022","journal-title":"Front Pharm."},{"key":"10.1016\/j.tice.2025.103263_bib35","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1186\/s13287-022-03140-3","article-title":"Runx2 and Nell-1 in dental follicle progenitor cells regulate bone remodeling and tooth eruption","volume":"13","author":"Zeng","year":"2022","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.tice.2025.103263_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2024.109682","article-title":"StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-\u03baB\/p-ERK MAPK-NFATc1 signaling pathway","volume":"27","author":"Zhou","year":"2024","journal-title":"iScience"}],"container-title":["Tissue and Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816625005452?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816625005452?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T05:29:55Z","timestamp":1773898195000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0040816625005452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":36,"alternative-id":["S0040816625005452"],"URL":"https:\/\/doi.org\/10.1016\/j.tice.2025.103263","relation":{},"ISSN":["0040-8166"],"issn-type":[{"value":"0040-8166","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"U0126 induces osteoclast differentiation via the p38-NFATc-1 signaling pathway","name":"articletitle","label":"Article Title"},{"value":"Tissue and Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tice.2025.103263","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"103263"}}