{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T23:58:44Z","timestamp":1793923124396,"version":"4.8.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004933","name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Toxinas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004933","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007762","name":"National Institute of Translational Science and Technology in Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","award":["2021\/14976\\u20136"],"award-info":[{"award-number":["2021\/14976\\u20136"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","award":["2021\/08029\\u20134"],"award-info":[{"award-number":["2021\/08029\\u20134"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Tissue and Cell"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.tice.2026.103463","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:24:14Z","timestamp":1773188654000},"page":"103463","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Comparative effects of Vitex agnus-castus and estradiol on bone tissue and osteoblasts under estrogen-deficient and oxidative stress conditions."],"prefix":"10.1016","volume":"101","author":[{"given":"Maria Carolina","family":"Coelho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Gabriel Plasier Guedes","family":"de Mello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin Tavares","family":"Camargo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger Rodrigo","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayuri Poli","family":"Suguimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma","family":"Siessere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0267-7643","authenticated-orcid":false,"given":"Karina Fittipaldi","family":"Bombonato-Prado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.tice.2026.103463_bib1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.ecl.2021.03.009","article-title":"Postmenopausal osteoporosis: latest guidelines","volume":"50","author":"Arceo-Mendoza","year":"2021","journal-title":"Endocrinol. Metab. Clin. North Am."},{"issue":"3","key":"10.1016\/j.tice.2026.103463_bib2","doi-asserted-by":"crossref","first-page":"749","DOI":"10.3390\/molecules30030749","article-title":"Bioactive potential and chemical composition of Vitex agnus-castus L. leaf extracts collected in Algeria: a combined in vitro and in silico approach","volume":"30","author":"Bramki","year":"2025","journal-title":"Molecules"},{"issue":"1","key":"10.1016\/j.tice.2026.103463_bib3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s11033-020-06096-w","article-title":"Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide","volume":"48","author":"Ceverino","year":"2021","journal-title":"Mol. Biol. Rep."},{"issue":"7","key":"10.1016\/j.tice.2026.103463_bib4","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1055\/s-2003-41122","article-title":"Phytoestrogens: recent developments","volume":"69","author":"Cos","year":"2003","journal-title":"Planta Med"},{"key":"10.1016\/j.tice.2026.103463_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.ctim.2019.08.024","article-title":"Vitex agnus-castus in premenstrual syndrome: a meta-analysis of double-blind randomised controlled trials","volume":"47","author":"Csupor","year":"2019","journal-title":"Complement. Ther. Med."},{"issue":"5","key":"10.1016\/j.tice.2026.103463_bib6","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1210\/jc.2019-00221","article-title":"Pharmacological management of osteoporosis in postmenopausal women: an Endocrine Society clinical practice guideline","volume":"104","author":"Eastell","year":"2019","journal-title":"J. Clin. Endocrinol. Metab."},{"issue":"3","key":"10.1016\/j.tice.2026.103463_bib7","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1159\/000021996","article-title":"Influence of aging on cortical and trabecular bone response to estradiol treatment in ovariectomized rats","volume":"44","author":"Gaumet","year":"1998","journal-title":"Gerontology"},{"issue":"1","key":"10.1016\/j.tice.2026.103463_bib8","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ab.2004.02.002","article-title":"An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction","volume":"329","author":"Gregory","year":"2004","journal-title":"Anal. Biochem"},{"issue":"10","key":"10.1016\/j.tice.2026.103463_bib9","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1007\/s00198-025-07592-0","article-title":"Nrf2 signaling pathway: focus on oxidative stress in osteoporosis","volume":"36","author":"Gu","year":"2025","journal-title":"Osteoporos. Int."},{"issue":"1","key":"10.1016\/j.tice.2026.103463_bib10","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.bbrc.2016.07.048","article-title":"Lack of SIRP\u03b1 phosphorylation and concomitantly reduced SHP-2\u2013PI3K\u2013Akt2 signaling decrease osteoblast differentiation","volume":"478","author":"Holm","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"22","key":"10.1016\/j.tice.2026.103463_bib11","doi-asserted-by":"crossref","first-page":"12139","DOI":"10.3390\/ijms252212139","article-title":"Primary osteoporosis induced by androgen and estrogen deficiency: the molecular and cellular perspective on pathophysiological mechanisms and treatments","volume":"25","author":"Hsu","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"4","key":"10.1016\/j.tice.2026.103463_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0154367","article-title":"Cortical bone morphological and trabecular bone microarchitectural changes in the mandible and femoral neck of ovariectomized rats","volume":"11","author":"Hsu","year":"2016","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.tice.2026.103463_bib13","doi-asserted-by":"crossref","first-page":"3772","DOI":"10.3390\/ijms24043772","article-title":"Oxidative stress and inflammation in osteoporosis: molecular mechanisms involved and the relationship with microRNAs","volume":"24","author":"Iantomasi","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"6","key":"10.1016\/j.tice.2026.103463_bib14","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1080\/14786410701592612","article-title":"Gynecological efficacy and chemical investigation of Vitex agnus-castus L. fruits growing in Egypt","volume":"22","author":"Ibrahim","year":"2008","journal-title":"Nat. Prod. Res."},{"issue":"14","key":"10.1016\/j.tice.2026.103463_bib15","doi-asserted-by":"crossref","first-page":"6973","DOI":"10.3390\/ijms26146973","article-title":"Daidzein and genistein: natural phytoestrogens with potential applications in hormone replacement therapy","volume":"26","author":"Intharuksa","year":"2025","journal-title":"Int. J. Mol. Sci."},{"issue":"10","key":"10.1016\/j.tice.2026.103463_bib16","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1055\/s-2003-45105","article-title":"Evidence for estrogen receptor beta-selective activity of Vitex agnus-castus and isolated flavones","volume":"69","author":"Jarry","year":"2003","journal-title":"Planta Med"},{"issue":"15","key":"10.1016\/j.tice.2026.103463_bib17","doi-asserted-by":"crossref","first-page":"8053","DOI":"10.3390\/ijms25158053","article-title":"Bisphosphonate-related osteonecrosis of the jaw and oral microbiome: clinical risk factors, pathophysiology and treatment options","volume":"25","author":"Jelin-Uhlig","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tice.2026.103463_bib18","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.bone.2016.03.016","article-title":"Estrogen receptors alpha and beta in bone","volume":"87","author":"Khalid","year":"2016","journal-title":"Bone"},{"key":"10.1016\/j.tice.2026.103463_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.jsbmb.2025.106740","article-title":"Phytoestrogens as potential anti-osteoporosis nutraceuticals: major sources and mechanisms of action","volume":"251","author":"Khan","year":"2025","journal-title":"J. Steroid Biochem. Mol. Biol."},{"issue":"2","key":"10.1016\/j.tice.2026.103463_bib20","doi-asserted-by":"crossref","first-page":"843","DOI":"10.3892\/ijo.2014.2454","article-title":"Involvement of histone H3 phosphorylation via the activation of p38 MAPK pathway and intracellular redox status in cytotoxicity of HL-60 cells induced by Vitex agnus-castus fruit extract","volume":"45","author":"Kikuchi","year":"2014","journal-title":"Int. J. Oncol."},{"issue":"11","key":"10.1016\/j.tice.2026.103463_bib21","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1080\/10408398.2013.789823","article-title":"Bioactivation of phytoestrogens: intestinal bacteria and health","volume":"56","author":"Landete","year":"2016","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.tice.2026.103463_bib22","article-title":"Site-specific and time-course changes of postmenopausal osteoporosis in rat mandible: comparative study with femur","volume":"9","author":"Lee","year":"2019","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.tice.2026.103463_bib23","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1078\/0944-7113-00331","article-title":"Isolation of linoleic acid as an estrogenic compound from the fruits of Vitex agnus-castus L","volume":"11","author":"Liu","year":"2004","journal-title":"Phytomedicine"},{"key":"10.1016\/j.tice.2026.103463_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2022.116501","article-title":"Site-specific characteristics of bone and progenitor cells in control and ovariectomized rats","volume":"163","author":"Liu","year":"2022","journal-title":"Bone"},{"issue":"4","key":"10.1016\/j.tice.2026.103463_bib25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"3","key":"10.1016\/j.tice.2026.103463_bib26","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.bone.2006.10.014","article-title":"The antioxidant effect of estrogen and selective estrogen receptor modulators in the inhibition of osteocyte apoptosis in vitro","volume":"40","author":"Mann","year":"2007","journal-title":"Bone"},{"issue":"2","key":"10.1016\/j.tice.2026.103463_bib27","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3390\/antiox12020373","article-title":"Oxidative stress and natural antioxidants in osteoporosis: novel preventive and therapeutic approaches","volume":"12","author":"Marcucci","year":"2023","journal-title":"Antioxidants"},{"issue":"1","key":"10.1016\/j.tice.2026.103463_bib28","doi-asserted-by":"crossref","DOI":"10.1515\/jcim-2018-0053","article-title":"The effects of Vitex agnus-castus on menstrual bleeding: a systematic review and meta-analysis","volume":"17","author":"Mollazadeh","year":"2019","journal-title":"J. Complement. Integr. Med."},{"issue":"10","key":"10.1016\/j.tice.2026.103463_bib29","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1001\/jama.2025.6003","article-title":"Osteoporosis: a review","volume":"334","author":"Morin","year":"2025","journal-title":"JAMA"},{"issue":"6","key":"10.1016\/j.tice.2026.103463_bib30","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/jbmr.5650020617","article-title":"Bone histomorphometry: standardization of nomenclature, symbols, and units","volume":"2","author":"Parfitt","year":"1987","journal-title":"J. Bone Miner. Res."},{"issue":"7","key":"10.1016\/j.tice.2026.103463_bib31","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.1002\/ptr.7861","article-title":"A review on phytoestrogens: current status and future direction","volume":"37","author":"Patra","year":"2023","journal-title":"Phytother. Res."},{"issue":"21","key":"10.1016\/j.tice.2026.103463_bib32","doi-asserted-by":"crossref","first-page":"4154","DOI":"10.3390\/molecules30214154","article-title":"Polyphenols and bone health: a comprehensive review of their role in osteoporosis prevention and treatment","volume":"30","author":"Perrone","year":"2025","journal-title":"Molecules"},{"issue":"6","key":"10.1016\/j.tice.2026.103463_bib33","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1002\/ptr.2711","article-title":"Vitex agnus-castus as prophylaxis for osteopenia after orchidectomy in rats compared with estradiol and testosterone supplementation","volume":"23","author":"Sehmisch","year":"2009","journal-title":"Phytother. Res."},{"issue":"3","key":"10.1016\/j.tice.2026.103463_bib34","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1111\/joa.13672","article-title":"Lycopene prevents bone loss in ovariectomized rats and increases the number of osteocytes and osteoblasts","volume":"241","author":"Semeghini","year":"2022","journal-title":"J. Anat."},{"issue":"3","key":"10.1016\/j.tice.2026.103463_bib35","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/ijos.2014.2","article-title":"Differentially expressed genes and signalling pathways are involved in mouse osteoblast-like MC3T3-E1 cells exposed to 17-\u03b2 estradiol","volume":"6","author":"Shang","year":"2014","journal-title":"Int. J. Oral. Sci."},{"issue":"8","key":"10.1016\/j.tice.2026.103463_bib36","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1002\/jbmr.4821","article-title":"Global epidemiology of hip fractures: secular trends in incidence rate, post-fracture treatment, and all-cause mortality","volume":"38","author":"Sing","year":"2023","journal-title":"J. Bone Miner. Res."},{"issue":"12","key":"10.1016\/j.tice.2026.103463_bib37","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1007\/s12272-022-01417-y","article-title":"Effects of phytoestrogens on reproductive organ health","volume":"45","author":"Swathi Krishna","year":"2022","journal-title":"Arch. Pharm. Res."},{"issue":"21","key":"10.1016\/j.tice.2026.103463_bib38","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1056\/NEJMcp2307353","article-title":"Postmenopausal osteoporosis","volume":"389","author":"Walker","year":"2023","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.tice.2026.103463_bib39","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s12038-021-00236-5","article-title":"17\u03b2-Estradiol alleviates oxidative damage in osteoblasts by regulating miR-320\/RUNX2 signaling pathway","volume":"46","author":"Xu","year":"2021","journal-title":"J. Biosci."},{"issue":"2","key":"10.1016\/j.tice.2026.103463_bib40","first-page":"768","article-title":"Possible modulation of the anxiogenic effects of Vitex agnus-castus by the serotonergic system","volume":"15","author":"Yaghmaei","year":"2012","journal-title":"Iran. J. Basic Med. Sci."},{"key":"10.1016\/j.tice.2026.103463_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2023.114834","article-title":"Oxidative stress: a common pathological state in a high-risk population for osteoporosis","volume":"163","author":"Zhang","year":"2023","journal-title":"Biomed. Pharm."},{"key":"10.1016\/j.tice.2026.103463_bib42","article-title":"Angiogenesis changes in ovariectomized rats with osteoporosis treated with estrogen replacement therapy","volume":"2019","author":"Zhang","year":"2019","journal-title":"Biomed. Res. Int."},{"issue":"3","key":"10.1016\/j.tice.2026.103463_bib43","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1002\/ptr.8451","article-title":"The potential mechanism of soy isoflavones in treating osteoporosis: focusing on bone metabolism and oxidative stress","volume":"39","author":"Zhou","year":"2025","journal-title":"Phytother. Res."}],"container-title":["Tissue and Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816626001564?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816626001564?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T02:52:43Z","timestamp":1778727163000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0040816626001564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":43,"alternative-id":["S0040816626001564"],"URL":"https:\/\/doi.org\/10.1016\/j.tice.2026.103463","relation":{},"ISSN":["0040-8166"],"issn-type":[{"value":"0040-8166","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative effects of Vitex agnus-castus and estradiol on bone tissue and osteoblasts under estrogen-deficient and oxidative stress conditions.","name":"articletitle","label":"Article Title"},{"value":"Tissue and Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tice.2026.103463","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"103463"}}