{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:22:06Z","timestamp":1787458926663,"version":"build-2736575974"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Tissue and Cell"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.tice.2026.103823","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:02:05Z","timestamp":1785250925000},"page":"103823","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"P1","title":["Unveiling the dynamic transcriptomic changes in and replicative senescence balance of hUC-MSCs: A comparative study of serum-containing versus serum-free culture supplements"],"prefix":"10.1016","volume":"104","author":[{"given":"Huan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenqing","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tice.2026.103823_bib1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.5187\/jast.2022.e114","article-title":"The roles of growth factors and hormones in the regulation of muscle satellite cells for cultured meat production","volume":"65","author":"Ahmad","year":"2023","journal-title":"J. Anim. Sci. Technol."},{"key":"10.1016\/j.tice.2026.103823_bib2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/s13287-018-0863-8","article-title":"Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience","volume":"9","author":"Becherucci","year":"2018","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib3","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1186\/s13287-017-0688-x","article-title":"Restoring the quantity and quality of elderly human mesenchymal stem cells for autologous cell-based therapies","volume":"8","author":"Block","year":"2017","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41580-022-00510-w","article-title":"Ageing and rejuvenation of tissue stem cells and their niches","volume":"24","author":"Brunet","year":"2023","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"10.1016\/j.tice.2026.103823_bib5","doi-asserted-by":"crossref","first-page":"2396","DOI":"10.1182\/blood.V98.8.2396","article-title":"Identification of mesenchymal stem\/progenitor cells in human first-trimester fetal blood, liver, and bone marrow","volume":"98","author":"Campagnoli","year":"2001","journal-title":"Blood"},{"key":"10.1016\/j.tice.2026.103823_bib6","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/cpr.12039","article-title":"Epiregulin can promote proliferation of stem cells from the dental apical papilla via MEK\/Erk and JNK signalling pathways","volume":"46","author":"Cao","year":"2013","journal-title":"Cell. Prolif."},{"key":"10.1016\/j.tice.2026.103823_bib7","doi-asserted-by":"crossref","DOI":"10.1186\/s13287-025-04835-z","article-title":"Differential effects of fetal bovine serum and human platelet lysate on mesenchymal stromal cell-mediated support of hematopoietic stem\/progenitor cells: a functional and transcriptomic analysis","author":"Carreira","year":"2025","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib8","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2024.1400347","article-title":"The issue of heterogeneity of MSC-based advanced therapy medicinal products-a review","volume":"12","author":"\u010cesnik","year":"2024","journal-title":"Front. Cell. Dev. Biol."},{"key":"10.1016\/j.tice.2026.103823_bib9","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1186\/s12967-024-05294-5","article-title":"Unveiling heterogeneity in MSCs: exploring marker-based strategies for defining MSC subpopulations","volume":"22","author":"Chen","year":"2024","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.tice.2026.103823_bib10","doi-asserted-by":"crossref","DOI":"10.34133\/bmr.0152","article-title":"Insulin-like growth factor 2 secreted from mesenchymal stem cells with high glutathione levels alleviates osteoarthritis via paracrine rejuvenation of senescent chondrocytes","volume":"29","author":"Cho","year":"2025","journal-title":"Biomater. Res."},{"key":"10.1016\/j.tice.2026.103823_bib11","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1109\/TCBB.2018.2873010","article-title":"Differential expression analysis of RNA-seq reads: overview, taxonomy, and tools","volume":"17","author":"Chowdhury","year":"2020","journal-title":"IEEE\/ACM Trans. Comput. Biol. Bioinform"},{"key":"10.1016\/j.tice.2026.103823_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/cells9092143","article-title":"miR-9-mediated inhibition of EFEMP1 contributes to the acquisition of pro-tumoral properties in normal fibroblasts","volume":"9","author":"Cosentino","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.tice.2026.103823_bib13","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1186\/s13287-023-03589-w","article-title":"Media matters: culture medium-dependent hypervariable phenotype of mesenchymal stromal cells","volume":"14","author":"Fitzgerald","year":"2023","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib14","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1097\/00007890-196803000-00009","article-title":"Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues","volume":"6","author":"Friedenstein","year":"1968","journal-title":"Transplantation"},{"key":"10.1016\/j.tice.2026.103823_bib15","doi-asserted-by":"crossref","DOI":"10.3390\/cells8080784","article-title":"Mesenchymal stem cell migration and tissue repair","volume":"8","author":"Fu","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.tice.2026.103823_bib16","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.tcb.2018.02.001","article-title":"Hallmarks of cellular senescence","volume":"28","author":"Hernandez-Segura","year":"2018","journal-title":"Trends Cell. Biol."},{"key":"10.1016\/j.tice.2026.103823_bib17","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/2116367","article-title":"Collagen modulates the biological characteristics of WJ-MSCs in basal and osteoinduced conditions","volume":"2022","author":"Hiew","year":"2022","journal-title":"Stem Cells Int."},{"key":"10.1016\/j.tice.2026.103823_bib18","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.781021","article-title":"Cross-tissue characterization of heterogeneities of mesenchymal stem cells and their differentiation potentials","volume":"9","author":"Hou","year":"2021","journal-title":"Front. Cell. Dev. Biol."},{"key":"10.1016\/j.tice.2026.103823_bib19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1186\/s13287-020-01723-6","article-title":"Human bone marrow mesenchymal stem cell-derived exosomes stimulate cutaneous wound healing mediates through TGF-\u03b2\/Smad signaling pathway","volume":"11","author":"Jiang","year":"2020","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib20","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines8050101","article-title":"The role of IL-6 in SKIN FIBROSIS AND CUTANEOUS WOUND HEaling","volume":"8","author":"Johnson","year":"2020","journal-title":"Biomedicines"},{"key":"10.1016\/j.tice.2026.103823_bib21","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241713408","article-title":"EGF, TGF-\u03b1 and amphiregulin differently regulate endometrium-derived mesenchymal stromal\/stem cells","volume":"24","author":"Kamentseva","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tice.2026.103823_bib22","doi-asserted-by":"crossref","DOI":"10.3390\/ijms251910627","article-title":"Comparative analysis of serum and serum-free medium cultured mesenchymal stromal cells for cartilage repair","volume":"25","author":"Kang","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tice.2026.103823_bib23","doi-asserted-by":"crossref","first-page":"3806","DOI":"10.1016\/j.celrep.2020.02.062","article-title":"Mesenchymal stem and stromal cells harness macrophage-derived amphiregulin to maintain tissue homeostasis","volume":"30","author":"Ko","year":"2020","journal-title":"Cell. Rep."},{"key":"10.1016\/j.tice.2026.103823_bib24","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1016\/j.cell.2025.03.011","article-title":"From geroscience to precision geromedicine: understanding and managing aging","volume":"188","author":"Kroemer","year":"2025","journal-title":"Cell"},{"key":"10.1016\/j.tice.2026.103823_bib25","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1089\/cell.2021.0050","article-title":"Differential development of umbilical cord-derived mesenchymal stem cells during long-term maintenance in fetal bovine serum-supplemented medium and xeno- and serum-free culture medium","volume":"23","author":"Le","year":"2021","journal-title":"Cell. Reprogram"},{"key":"10.1016\/j.tice.2026.103823_bib26","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1093\/stcltm\/szac050","article-title":"Chemokine assay matrix defines the potency of human bone marrow mesenchymal stromal cells","volume":"11","author":"Lipat","year":"2022","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.tice.2026.103823_bib27","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-72386-z","article-title":"Maternal and neonatal factors' effects on wharton's jelly mesenchymal stem cell yield","volume":"14","author":"Mahmoud","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.tice.2026.103823_bib28","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1267550","article-title":"Age-related changes in human bone marrow mesenchymal stromal cells: morphology, gene expression profile, immunomodulatory activity and miRNA expression","volume":"14","author":"Massaro","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.tice.2026.103823_bib29","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1002\/sctm.19-0044","article-title":"Challenges in clinical development of mesenchymal stromal\/stem cells: concise review","volume":"8","author":"Mastrolia","year":"2019","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.tice.2026.103823_bib30","doi-asserted-by":"crossref","DOI":"10.1042\/bst019029s","article-title":"Isolation, culture and characterisation of fibroblast-like cells derived from the Wharton's jelly portion of human umbilical cord","volume":"19","author":"McElreavey","year":"1991","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.tice.2026.103823_bib31","doi-asserted-by":"crossref","DOI":"10.3390\/cells10040886","article-title":"Towards physiologic culture approaches to improve standard cultivation of mesenchymal stem cells","volume":"10","author":"Nikolits","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.tice.2026.103823_bib32","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/j.1524-475X.2011.00726.x","article-title":"Clinical efficacy of basic fibroblast growth factor on pressure ulcers: case-control pairing study using a new evaluation method","volume":"19","author":"Ohura","year":"2011","journal-title":"Wound Repair. Regen."},{"key":"10.1016\/j.tice.2026.103823_bib33","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1186\/s13287-022-02815-1","article-title":"Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol","volume":"13","author":"Palombella","year":"2022","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib34","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1146\/annurev-cellbio-111315-125341","article-title":"Focal adhesion-independent cell migration","volume":"32","author":"Paluch","year":"2016","journal-title":"Annu. Rev. Cell. Dev. Biol."},{"key":"10.1016\/j.tice.2026.103823_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.tice.2022.101791","article-title":"Effect of miR-381-3p\/FGF7 axis on the osteogenic differentiation of bone marrow mesenchymal stem cells through MEK\/ERK signaling pathway","volume":"76","author":"Qiu","year":"2022","journal-title":"Tissue Cell."},{"key":"10.1016\/j.tice.2026.103823_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.mad.2020.111288","article-title":"Stem cell aging: the upcoming era of proteins and metabolites","volume":"190","author":"Sch\u00fcler","year":"2020","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.tice.2026.103823_bib37","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13287-020-02121-8","article-title":"Deconstructing transcriptional variations and their effects on immunomodulatory function among human mesenchymal stromal cells","volume":"12","author":"Sun","year":"2021","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib38","doi-asserted-by":"crossref","DOI":"10.3390\/cells11071210","article-title":"Equilibrium among inflammatory factors determines human MSC-mediated immunosuppressive effect","volume":"11","author":"Suzdaltseva","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.tice.2026.103823_bib39","doi-asserted-by":"crossref","DOI":"10.1002\/ctm2.650","article-title":"Single-cell transcriptome atlas of human mesenchymal stem cells exploring cellular heterogeneity","volume":"11","author":"Wang","year":"2021","journal-title":"Clin. Transl. Med."},{"key":"10.1016\/j.tice.2026.103823_bib40","doi-asserted-by":"crossref","first-page":"4192","DOI":"10.7150\/ijbs.61950","article-title":"Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells","volume":"17","author":"Wang","year":"2021","journal-title":"Int. J. Biol. Sci."},{"key":"10.1016\/j.tice.2026.103823_bib41","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1093\/stcltm\/szac004","article-title":"Mesenchymal stem\/stromal cell senescence: hallmarks, mechanisms, and combating strategies","volume":"11","author":"Weng","year":"2022","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.tice.2026.103823_bib42","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.jcyt.2020.03.431","article-title":"Derivation of clinical-grade mesenchymal stromal cells from umbilical cord under chemically defined culture condition - platform for future clinical application","volume":"22","author":"Wu","year":"2020","journal-title":"Cytotherapy"},{"key":"10.1016\/j.tice.2026.103823_bib43","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/s13287-020-01641-7","article-title":"Chemical-defined medium supporting the expansion of human mesenchymal stem cells","volume":"11","author":"Xu","year":"2020","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib44","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s13287-018-0876-3","article-title":"Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro","volume":"9","author":"Yang","year":"2018","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib45","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s13287-020-02055-1","article-title":"Single-cell transcriptome analysis of uncultured human umbilical cord mesenchymal stem cells","volume":"12","author":"Zhang","year":"2021","journal-title":"Stem Cell. Res. Ther."},{"key":"10.1016\/j.tice.2026.103823_bib46","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1089\/107632701300062859","article-title":"Multilineage cells from human adipose tissue: implications for cell-based therapies","volume":"7","author":"Zuk","year":"2001","journal-title":"Tissue Eng."}],"container-title":["Tissue and Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816626005173?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0040816626005173?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:07:24Z","timestamp":1787458044000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0040816626005173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":46,"alternative-id":["S0040816626005173"],"URL":"https:\/\/doi.org\/10.1016\/j.tice.2026.103823","relation":{},"ISSN":["0040-8166"],"issn-type":[{"value":"0040-8166","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Unveiling the dynamic transcriptomic changes in and replicative senescence balance of hUC-MSCs: A comparative study of serum-containing versus serum-free culture supplements","name":"articletitle","label":"Article Title"},{"value":"Tissue and Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tice.2026.103823","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"103823"}}