{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T21:48:52Z","timestamp":1791668932163,"version":"4.3.4"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Tissue and Cell"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.tice.2026.104028","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:58:33Z","timestamp":1791475113000},"page":"104028","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"P3","title":["An ozone injectable solution reduces structural cartilage degeneration in an ACLT rat model"],"prefix":"10.1016","volume":"104","author":[{"given":"Elif","family":"\u00dcnl\u00fc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet \u00dcmit","family":"Sabanc\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nihal Y.","family":"G\u00fcl Satar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onur","family":"Onguncan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6ner","family":"S\u00f6nmez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabire","family":"G\u00fcler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aybike S.","family":"Bozkurt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazmiye","family":"G\u00fcne\u015f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tice.2026.104028_bib1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1080\/01919512.2021.1904205","article-title":"New antibacterial agent: nanobubble ozone stored in liposomes: the antibacterial activity of nanobubble ozone in liposomes and their thymol solutions","volume":"43","author":"Alkan","year":"2021","journal-title":"Ozone Sci. Eng."},{"key":"10.1016\/j.tice.2026.104028_bib2","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.addr.2010.01.001","article-title":"Potential of endogenous regenerative technology for in situ regenerative medicine","volume":"62","author":"Anitua","year":"2010","journal-title":"Adv. Drug. Deliv. Rev."},{"key":"10.1016\/j.tice.2026.104028_bib3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1479-5876-9-66","article-title":"Ozone acting on human blood yields a hormetic dose-response relationship","volume":"9","author":"Bocci","year":"2011","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.tice.2026.104028_bib4","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.arcmed.2005.08.006","article-title":"Scientific and medical aspects of ozone therapy. State of the art","volume":"37","author":"Bocci","year":"2006","journal-title":"Arch. Med. Res."},{"key":"10.1016\/j.tice.2026.104028_bib5","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.knee.2025.03.010","article-title":"Corticosteroids versus platelet-rich plasma injections for knee osteoarthritis: Where is there more evidence? A systematic review of 60 years of literature","volume":"55","author":"Boffa","year":"2025","journal-title":"Knee"},{"key":"10.1016\/j.tice.2026.104028_bib6","first-page":"161","article-title":"Disc herniation and knee arthritis as chronic oxidative stress diseases: the therapeutic role of oxygen ozone therapy","volume":"4","author":"Borrelli","year":"2015","journal-title":"J. Arthritis"},{"key":"10.1016\/j.tice.2026.104028_bib7","doi-asserted-by":"crossref","first-page":"975","DOI":"10.2147\/IJN.S68861","article-title":"Liposomes as nanomedical devices","author":"Bozzuto","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.tice.2026.104028_bib8","doi-asserted-by":"crossref","DOI":"10.1590\/acb361201","article-title":"Use of hyaluronic acid associated with triamcinolone acetonide or ozone gas in the treatment of induced osteoarthritis in rabbits","volume":"36","author":"Brombini","year":"2021","journal-title":"Acta Cir. Bras."},{"key":"10.1016\/j.tice.2026.104028_bib9","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.actbio.2019.03.010","article-title":"Intra-articular targeting of nanomaterials for the treatment of osteoarthritis","volume":"93","author":"Brown","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.tice.2026.104028_bib10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s40266-019-00660-1","article-title":"Safety of oral non-selective non-steroidal anti-inflammatory drugs in osteoarthritis: what does the literature say?","volume":"36","author":"Cooper","year":"2019","journal-title":"Drugs & Aging"},{"key":"10.1016\/j.tice.2026.104028_bib11","doi-asserted-by":"crossref","first-page":"576","DOI":"10.20344\/amp.10330","article-title":"Ozone Therapy in Knee Osteoarthritis: A Systematic Review","volume":"31","author":"Costa","year":"2018","journal-title":"Acta Med. Port."},{"key":"10.1016\/j.tice.2026.104028_bib12","first-page":"33","article-title":"Comparing therapeutic effects of intraarticular ozone and hyaluronic acid in the rat model of experimental knee osteoarthritis","author":"Dayan\u0131r","year":"2022","journal-title":"Gazi Med. J."},{"key":"10.1016\/j.tice.2026.104028_bib13","first-page":"109","article-title":"The efficacy of intra-articular lidocaine administration in chronic knee pain due to osteoarthritis: a randomized, double-blind, controlled study","volume":"36","author":"Eker","year":"2017","journal-title":"Anaesth. Crit. Care Pain. Med."},{"key":"10.1016\/j.tice.2026.104028_bib14","doi-asserted-by":"crossref","first-page":"S24","DOI":"10.1016\/j.joca.2010.05.030","article-title":"The OARSI histopathology initiative\u2013recommendations for histological assessments of osteoarthritis in the rat","volume":"18","author":"Gerwin","year":"2010","journal-title":"Osteoarthr. Cartil."},{"key":"10.1016\/j.tice.2026.104028_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2019.00192","article-title":"Hyaluronic acid: molecular mechanisms and therapeutic trajectory","volume":"6","author":"Gupta","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.tice.2026.104028_bib16","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.joca.2006.11.008","article-title":"Intra-articular injection of the cyclooxygenase-2 inhibitor parecoxib attenuates osteoarthritis progression in anterior cruciate ligament-transected knee in rats: role of excitatory amino acids","volume":"15","author":"Jean","year":"2007","journal-title":"Osteoarthr. Cartil."},{"key":"10.1016\/j.tice.2026.104028_bib17","doi-asserted-by":"crossref","DOI":"10.1142\/S0218957720500098","article-title":"Comparison of intra-articular application of polydeoxyribonucleic acid and hyaluronic acid in experimentally induced osteoarthritis in rats","volume":"23","author":"Karahan","year":"2020","journal-title":"J. Musculoskelet. Res."},{"key":"10.1016\/j.tice.2026.104028_bib18","first-page":"256","article-title":"Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research","volume":"20","author":"Kilkenny","year":"2010","journal-title":"J. Pharmacol. Pharmacother."},{"key":"10.1016\/j.tice.2026.104028_bib19","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1007\/s00264-017-3422-5","article-title":"Proliferative injection therapy for osteoarthritis: a systematic review","volume":"41","author":"Krsti\u010devi\u0107","year":"2017","journal-title":"Int. Orthop."},{"key":"10.1016\/j.tice.2026.104028_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0179185","article-title":"Comparison between intra-articular ozone and placebo in the treatment of knee osteoarthritis: A randomized, double-blinded, placebo-controlled study","volume":"12","author":"Lopes de Jesus","year":"2017","journal-title":"PLoS. One"},{"key":"10.1016\/j.tice.2026.104028_bib21","doi-asserted-by":"crossref","first-page":"523","DOI":"10.2106\/00004623-197153030-00009","article-title":"Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips: II. Correlation of morphology with biochemical and metabolic data","volume":"53","author":"Mankin","year":"1971","journal-title":"J. Bone Jt. Surg."},{"key":"10.1016\/j.tice.2026.104028_bib22","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.ejphar.2005.08.020","article-title":"Therapeutic efficacy of ozone in patients with diabetic foot","volume":"523","author":"Mart\u00ednez-S\u00e1nchez","year":"2005","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.tice.2026.104028_bib23","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202410060","article-title":"Liposome-Based Interventions in Knee Osteoarthritis","volume":"21","author":"Mitsou","year":"2025","journal-title":"Small"},{"key":"10.1016\/j.tice.2026.104028_bib24","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.semarthrit.2013.11.012","article-title":"A systematic review of recommendations and guidelines for the management of osteoarthritis: the chronic osteoarthritis management initiative of the US bone and joint initiative","volume":"43","author":"Nelson","year":"2014","journal-title":"Semin. Arthritis Rheum."},{"key":"10.1016\/j.tice.2026.104028_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.ocarto.2020.100066","article-title":"Characterization of OA development between sexes in the rat medial meniscal transection model","volume":"2","author":"Pucha","year":"2020","journal-title":"Osteoarthr. Cartil. Open."},{"key":"10.1016\/j.tice.2026.104028_bib26","doi-asserted-by":"crossref","first-page":"456S","DOI":"10.1177\/1947603520904759","article-title":"The influence of a single intra-articular lidocaine injection on the viability of articular cartilage in the knee","volume":"13","author":"Ravnihar","year":"2021","journal-title":"Cartilage"},{"key":"10.1016\/j.tice.2026.104028_bib27","doi-asserted-by":"crossref","first-page":"351","DOI":"10.2147\/IJN.S328090","article-title":"Nanobubble ozone stored in hyaluronic acid decorated liposomes: antibacterial, anti-SARS-CoV-2 effect and biocompatibility tests","author":"Sabanc\u0131","year":"2022","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.tice.2026.104028_bib28","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1136\/annrheumdis-2019-216515","article-title":"Global, regional and national burden of osteoarthritis 1990\u20132017: a systematic analysis of the Global Burden of Disease Study 2017","volume":"79","author":"Safiri","year":"2020","journal-title":"Ann. Rheum. Dis."},{"key":"10.1016\/j.tice.2026.104028_bib29","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/2045-9912-1-29","article-title":"Mechanisms of action involved in ozone therapy: is healing induced via a mild oxidative stress?","volume":"1","author":"Sagai","year":"2011","journal-title":"Med. Gas. Res."},{"key":"10.1016\/j.tice.2026.104028_bib30","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.bone.2012.02.012","article-title":"The role of synovitis in osteoarthritis pathogenesis","volume":"51","author":"Scanzello","year":"2012","journal-title":"Bone"},{"key":"10.1016\/j.tice.2026.104028_bib31","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1111\/jcpt.13669","article-title":"Pharmacokinetics, safety and efficacy of intra-articular non-steroidal anti-inflammatory drug injections for the treatment of osteoarthritis: A narrative review","volume":"47","author":"Selig","year":"2022","journal-title":"J. Clin. Pharm. Ther."},{"key":"10.1016\/j.tice.2026.104028_bib32","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.knee.2022.03.003","article-title":"Intra-articular ozone slows down the process of degeneration of articular cartilage in the knees of rats with osteoarthritis","volume":"35","author":"Spassim","year":"2022","journal-title":"Knee"},{"key":"10.1016\/j.tice.2026.104028_bib33","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1016\/j.joca.2019.03.001","article-title":"Joint unloading inhibits articular cartilage degeneration in knee joints of a monosodium iodoacetate-induced rat model of osteoarthritis","volume":"27","author":"Takahashi","year":"2019","journal-title":"Osteoarthr. Cartil."},{"key":"10.1016\/j.tice.2026.104028_bib34","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1089\/bio.2023.0073","article-title":"Evaluation of Cryopreserved Ram Sperm with Nano-Ozone Solution and Post-Thaw Life Span by Flow Cytometric Analysis","volume":"22","author":"Toker","year":"2024","journal-title":"Biopreserv. Biobank."},{"key":"10.1016\/j.tice.2026.104028_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.rvsc.2025.105798","article-title":"Chondroprotective effects of ozone and hyaluronic acid in rat knee osteoarthritis: Comparison of intra-articular and systemic administration","author":"Unlu","year":"2025","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.tice.2026.104028_bib36","first-page":"103","article-title":"Effects of surgically induced instability on rat knee articular cartilage","volume":"134","author":"Williams","year":"1982","journal-title":"J. Anat."},{"key":"10.1016\/j.tice.2026.104028_bib37","first-page":"67","article-title":"Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model","volume":"22","author":"Xu","year":"2020","journal-title":"J. Orthop. Transl."},{"key":"10.1016\/j.tice.2026.104028_bib38","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.12998\/wjcc.v8.i11.2280","article-title":"Role of IL-17 gene polymorphisms in osteoarthritis: A meta-analysis based on observational studies","volume":"8","author":"Yang","year":"2020","journal-title":"World J. Clin. Cases"},{"key":"10.1016\/j.tice.2026.104028_bib39","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1007\/s00590-014-1437-9","article-title":"An evaluation of the delayed effect of intra-articular injections of lidocaine (2%) on articular cartilage: an experimental study in rabbits","volume":"24","author":"Yazdi","year":"2014","journal-title":"Eur. J. Orthop. Surg. Traumatol."},{"key":"10.1016\/j.tice.2026.104028_bib40","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1007\/s00264-020-04871-9","article-title":"The effects of systemic ozone application and hyperbaric oxygen therapy on knee osteoarthritis: an experimental study in rats","volume":"45","author":"Y\u0131lmaz","year":"2021","journal-title":"Int. Orthop."},{"key":"10.1016\/j.tice.2026.104028_bib41","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ejpb.2023.10.010","article-title":"Overcoming delivery barriers for RNA therapeutics in the treatment of rheumatoid arthritis","volume":"192","author":"Zhang","year":"2023","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.tice.2026.104028_bib42","doi-asserted-by":"crossref","first-page":"431","DOI":"10.3389\/fendo.2019.00431","article-title":"Emerging trend in the pharmacotherapy of osteoarthritis","volume":"10","author":"Zhang","year":"2019","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.tice.2026.104028_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.nantod.2022.101675","article-title":"Improvement of radiotherapy with an ozone-carried liposome nano-system for synergizing cancer immune checkpoint blockade","volume":"47","author":"Zheng","year":"2022","journal-title":"Nano Today"}],"container-title":["Tissue and Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004081662600724X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004081662600724X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T21:14:36Z","timestamp":1791666876000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S004081662600724X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":43,"alternative-id":["S004081662600724X"],"URL":"https:\/\/doi.org\/10.1016\/j.tice.2026.104028","relation":{},"ISSN":["0040-8166"],"issn-type":[{"value":"0040-8166","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An ozone injectable solution reduces structural cartilage degeneration in an ACLT rat model","name":"articletitle","label":"Article Title"},{"value":"Tissue and Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tice.2026.104028","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"104028"}}