{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:55:21Z","timestamp":1790621721258,"version":"4.1.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2006,6]]},"DOI":"10.1016\/j.tiv.2005.08.018","type":"journal-article","created":{"date-parts":[[2005,10,11]],"date-time":"2005-10-11T11:23:11Z","timestamp":1129029791000},"page":"480-489","source":"Crossref","is-referenced-by-count":50,"title":["Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay"],"prefix":"10.1016","volume":"20","author":[{"given":"Dijoux","family":"Nathalie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guingand","family":"Yannick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bourgeois","family":"Caroline","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durand","family":"Sandrine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fromageot","family":"Claude","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Combe","family":"Corinne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferret","family":"Pierre-Jacques","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2005.08.018_bib1","unstructured":"2000\/33\/EC, Commission Directive, 2000. Commission Directive 2000\/33\/EC of 25 April 2000 adapting to technical progress for the 27th time Council Directive 67\/548 E on the classification, packaging and labelling of dangerous substances, 2000. Annex V method B41 phototoxicity\u2014in vitro 3T3 NRU phototoxicity test. Official Journal of European Communities, L136, 90."},{"key":"10.1016\/j.tiv.2005.08.018_bib2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0887-2333(91)90016-7","article-title":"Toxic effects of non-steroidal anti-inflammatory drugs in a human intestinal epithelial cell line (HCT-8), as assessed by the MTT and neutral red assays","volume":"5","author":"Allen","year":"1991","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib3","first-page":"339","article-title":"In vitro assessment of phototoxicity","volume":"10","author":"Api","year":"1997","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib4","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1600-0781.1997.tb00105.x","article-title":"Use of dermal equivalent and skin equivalent models for identifying phototoxic compounds in vitro","volume":"13","author":"Augustin","year":"1997","journal-title":"Photodermatology, Photoimmunology and Photomedicine"},{"key":"10.1016\/j.tiv.2005.08.018_bib5","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1023\/A:1007604612003","article-title":"Development of a highly sensitive in vitro phototoxicity assay using the SkinEthic reconstructed human epidermis","volume":"16","author":"Bernard","year":"2000","journal-title":"Cellular and Biological Toxicology"},{"key":"10.1016\/j.tiv.2005.08.018_bib6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0378-4274(85)90046-3","article-title":"Toxicity determined in vitro by morphological alterations and neutral red absorption","volume":"24","author":"Borenfreund","year":"1985","journal-title":"Toxicology Letters"},{"key":"10.1016\/j.tiv.2005.08.018_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0887-2333(88)90030-6","article-title":"Comparisons of two in vitro cytotoxicity assays\u2014the neutral red (NR) and tetrazolium MTT tests","volume":"2","author":"Borenfreund","year":"1988","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib8","first-page":"247","article-title":"The use of human keratinocytes in the EU\/COLIPA international in vitro phototoxicity test validation study and the ECVAM\/COLIPA study on UV filter chemicals","volume":"27","author":"Clothier","year":"1999","journal-title":"ATLA"},{"key":"10.1016\/j.tiv.2005.08.018_bib9","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0887-2333(92)90004-B","article-title":"Prediction of eye irritancy potential of surfactants by cytotoxicity tests in vitro on cultures of human skin fibroblasts and keratinocytes","volume":"6","author":"Cornelis","year":"1992","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib10","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0022-1759(86)90368-6","article-title":"Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability","volume":"89","author":"Denizot","year":"1986","journal-title":"Journal of Immunological Methods"},{"key":"10.1016\/j.tiv.2005.08.018_bib11","unstructured":"Directive 67\/548\/EEC, 2004. Annex V. Part B.41\u2014Phototoxicity\u2014In vitro 3T3 NRU phototoxicity test."},{"key":"10.1016\/j.tiv.2005.08.018_bib12","first-page":"111","article-title":"Quantitative in vitro assessment of phototoxicity using a human skin model, Skin2","volume":"10","author":"Edwards","year":"1994","journal-title":"Photodermatology, Photoimmunology and Photomedicine."},{"key":"10.1016\/j.tiv.2005.08.018_bib13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0190-9622(83)70016-2","article-title":"Phototoxicity and photoallergy in man","volume":"8","author":"Epstein","year":"1983","journal-title":"Journal of American Academy of Dermatology"},{"key":"10.1016\/j.tiv.2005.08.018_bib14","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S1011-1344(01)00207-X","article-title":"Photosensitized oxidation of membrane lipids: reaction pathways, cytotoxic effects, and cytoprotective mechanisms","volume":"63","author":"Girotti","year":"2001","journal-title":"Journal of Photochemistry and Photobiology B"},{"key":"10.1016\/j.tiv.2005.08.018_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/105172301300055115","article-title":"In vitro testing for phototoxic potential using the epiderm 3-D reconstructed human skin model","volume":"11","author":"Jones","year":"2001","journal-title":"Toxicological Methods"},{"key":"10.1016\/j.tiv.2005.08.018_bib16","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1111\/j.1600-0781.1999.tb00094.x","article-title":"In vitro phototoxicity of new quinolones: production of active oxygen species and photosensitized lipid peroxidation","volume":"16","author":"Kawada","year":"1999","journal-title":"Photodermatology, Photoimmunology and Photomedicine"},{"key":"10.1016\/j.tiv.2005.08.018_bib17","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0024-3205(94)00746-2","article-title":"MTT-assay and neutral red release (NRR)-assay: relative role in the prediction of the irritancy potential of surfactants","volume":"55","author":"Korting","year":"1994","journal-title":"Life Science"},{"key":"10.1016\/j.tiv.2005.08.018_bib18","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0887-2333(93)90117-N","article-title":"A scheme for in vitro screening of substances for photoallergenic potential","volume":"7","author":"Lovell","year":"1993","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib19","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/0887-2333(91)90072-L","article-title":"Development of an in vitro test system with human skin cells for evaluation of phototoxicity","volume":"5","author":"Maier","year":"1991","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib20","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1111\/j.1751-1097.1998.tb05217.x","article-title":"Fluoroquinolone antimicrobials: singlet oxygen, superoxide and phototoxicity","volume":"67","author":"Martinez","year":"1998","journal-title":"Photochemistry and Photobiology"},{"key":"10.1016\/j.tiv.2005.08.018_bib21","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/0887-2333(91)90070-T","article-title":"Effects of exposure period of acetylsalicylic acid, paracetamol and isopropanol on L929 cytotoxicity","volume":"5","author":"Nordin","year":"1991","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib22","unstructured":"OECD, 2004. OECD Guideline for testing of chemicals, no. 432: In vitro 3T3 NRU phototoxicity test. Organisation for Economic Co-operation and Development, Paris, France."},{"key":"10.1016\/j.tiv.2005.08.018_bib23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0887-2333(94)00209-D","article-title":"Effect of high polyol concentrations on the neutral red absorption assay and tetrazolium-MTT test of rat hepatocytes in primary culture","volume":"9","author":"Olivier","year":"1995","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib24","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/S1011-1344(01)00196-8","article-title":"Ocular phototoxicity","volume":"64","author":"Roberts","year":"2001","journal-title":"Journal of Photochemistry and Photobiology B: Biology"},{"key":"10.1016\/j.tiv.2005.08.018_bib25","unstructured":"Spielmann, H., Balls, M., Dupuis, J., Pape, W.J.W., De Silva, O., Holzhutter, H.-G., Gerberick, F., Liebsch, M., Lovell, W.W., Maurer, T., Pfannennbecker, U. 1998a. A study on UV filter chemicals from Annex VII of European Union Directive 76\/768\/EEC, in the 3T3 NRU phototoxicity test. ATLA 26."},{"key":"10.1016\/j.tiv.2005.08.018_bib26","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0887-2333(98)00006-X","article-title":"The international EU\/COLIPA in vitro phototoxicity validation study: results of phase II (blind trial). Part 1: The 3T3 NRU phototoxicity test","volume":"12","author":"Spielmann","year":"1998","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib27","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1006\/abbi.1997.0124","article-title":"Participation of reactive oxygen species in phototoxicity induced by quinolone antibacterial agents","volume":"342","author":"Umezawa","year":"1997","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"10.1016\/j.tiv.2005.08.018_bib28","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0887-2333(94)00200-E","article-title":"Comparison of three in vitro cytotoxicity assays for estimating surfactant ocular irritation","volume":"9","author":"Vian","year":"1995","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2005.08.018_bib29","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0014-827X(99)00116-0","article-title":"In vitro studies of the phototoxic potential of the antidepressant drugs amitriptyline and imipramine","volume":"55","author":"Viola","year":"2000","journal-title":"Farmaco"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233305001748?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233305001748?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T22:58:59Z","timestamp":1548111539000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233305001748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["S0887233305001748"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2005.08.018","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2006,6]]}}}