{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:34:39Z","timestamp":1790778879041,"version":"4.1.0"},"reference-count":17,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2009,3]]},"DOI":"10.1016\/j.tiv.2008.12.003","type":"journal-article","created":{"date-parts":[[2008,12,14]],"date-time":"2008-12-14T04:36:12Z","timestamp":1229229372000},"page":"333-337","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":40,"title":["Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane"],"prefix":"10.1016","volume":"23","author":[{"given":"T.","family":"Uchino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Takezawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Ikarashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2008.12.003_bib1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1034\/j.1600-0536.2000.042006344.x","article-title":"Use of local lymph node assay for the estimation of relative contact allergenic potency","volume":"42","author":"Basketter","year":"2000","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2008.12.003_bib2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/0887-2333(91)90099-Y","article-title":"The living skin equivalent: its manufacture, its organotypic properties and its response to irritants","volume":"5","author":"Bell","year":"1991","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2008.12.003_bib3","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0887-2333(03)00019-5","article-title":"Analysis of interleukin-1\u03b1(IL-1\u03b1) and interleukin-8(IL-8) expression and release in vitro reconstructed human epidermis for the prediction of in vivo skin irritation and\/or sensitization","volume":"17","author":"Coquette","year":"2003","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2008.12.003_bib4","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0887-2333(01)00029-7","article-title":"Moduration of phenotype, cytokine production and stimulatory function of CD34+ -derived DC by NiCl2 and SDS","volume":"15","author":"De Smedt","year":"2001","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.tiv.2008.12.003_bib5","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1600-065X.1991.tb00581.x","article-title":"Determination of IL1 alpha, IL1 beta and IL2 in biological media using specific enzyme immunometric assays","volume":"119","author":"Grassi","year":"1991","journal-title":"Immunological Reviews"},{"key":"10.1016\/j.tiv.2008.12.003_bib6","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/jat.876","article-title":"Differential stimulation of IgE production, STAT activation and cytokine and CD86 expression by 2,4-dinitorochlorobenzene and trimellitic anhydride","volume":"22","author":"Guo","year":"2002","journal-title":"Journal of Applied Toxicology"},{"key":"10.1016\/j.tiv.2008.12.003_bib7","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1007\/BF00278640","article-title":"A murine local lymph node assay for the identification of contact allergens","volume":"63","author":"Kimber","year":"1989","journal-title":"Archives of Toxicology"},{"key":"10.1016\/j.tiv.2008.12.003_bib8","first-page":"87","article-title":"Development and evaluation of the cultured skin model","volume":"17","author":"Morota","year":"1998","journal-title":"Tissue culture Research Communication"},{"key":"10.1016\/j.tiv.2008.12.003_bib9","first-page":"41","article-title":"Alternative to primary draize skin irritation test using cultured human skin model: comparison of six endpoints","volume":"6","author":"Morota","year":"1999","journal-title":"Alternative Test Experiment"},{"key":"10.1016\/j.tiv.2008.12.003_bib10","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1532\/IJH97.A10406","article-title":"In vitro generation of functional dendritic cells from human umbilical cord blood CD34+ cells by a 2-steo culture method","volume":"80","author":"Ryu","year":"2004","journal-title":"International Journal of Hematology"},{"key":"10.1016\/j.tiv.2008.12.003_bib11","doi-asserted-by":"crossref","first-page":"463","DOI":"10.3727\/000000004783983882","article-title":"Collagen vitrigel: a novel scaffold that can facilitate a three-dimensional culture for reconstructing organoids","volume":"13","author":"Takezawa","year":"2004","journal-title":"Cell Transplantation"},{"key":"10.1016\/j.tiv.2008.12.003_bib12","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1159\/000101325","article-title":"A protein-permeable scaffold of a collagen vitrigel membrane useful for reconstructing crosstalk models between two different cell types","volume":"185","author":"Takezawa","year":"2007","journal-title":"Cells Tissues Organs"},{"key":"10.1016\/j.tiv.2008.12.003_bib13","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jbiotec.2007.05.033","article-title":"Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo","volume":"131","author":"Takezawa","year":"2007","journal-title":"Journal Biotechnology"},{"key":"10.1016\/j.tiv.2008.12.003_bib14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1248\/yakushi.128.51","article-title":"Advantages of culture models utilizing substrata made of TOSHI (tissue\/organ sections for histopathology) or collagen vitrigel membrane and their application concept for drug development researches","volume":"128","author":"Takezawa","year":"2008","journal-title":"Yakugaku Zasshi"},{"key":"10.1016\/j.tiv.2008.12.003_bib15","doi-asserted-by":"crossref","first-page":"246","DOI":"10.5107\/sccj.41.4_246","article-title":"Construction of a three-dimensional human skin model consisting of keratinocytes, dendritic cells and fibroblasts and application of this model for alternative animal testing of immune-sensitizing compounds","volume":"41","author":"Uchino","year":"2007","journal-title":"Journal of society cosmetic chemists Japan"},{"key":"10.1016\/j.tiv.2008.12.003_bib16","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1248\/yakushi.128.45","article-title":"Construction of a three-dimensional human skin model involving dendritic cells and its application to skin sensitization test","volume":"128","author":"Uchino","year":"2008","journal-title":"Yakugaku Zasshi"},{"key":"10.1016\/j.tiv.2008.12.003_bib17","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0887-2333(03)00006-7","article-title":"Evaluation of the skin sensitization potential chemicals using expression of co-stimulatory molecules, CD54 and CD86, on the native THP-1 cell line","volume":"17","author":"Yoshida","year":"2003","journal-title":"Toxicology In Vitro"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233308002889?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233308002889?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:27:53Z","timestamp":1760135273000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233308002889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["S0887233308002889"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2008.12.003","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2009,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane","name":"articletitle","label":"Article Title"},{"value":"Toxicology in Vitro","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tiv.2008.12.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2008 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}