{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:48:53Z","timestamp":1792007333947,"version":"4.4.0"},"reference-count":15,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2010,3]]},"DOI":"10.1016\/j.tiv.2009.10.015","type":"journal-article","created":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T04:11:29Z","timestamp":1257048689000},"page":"411-416","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Use of in vitro testing to identify an unexpected skin sensitizing impurity in a commercial product: A case study"],"prefix":"10.1016","volume":"24","author":[{"given":"Andreas","family":"Natsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Gfeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Emter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2009.10.015_bib1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1080\/15476910600978038","article-title":"Activation of U937 cells by contact sensitizers: CD86 expression is independent of apoptosis","volume":"3","author":"Ade","year":"2006","journal-title":"Journal of Immunotoxicology"},{"key":"10.1016\/j.tiv.2009.10.015_bib2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.yrtph.2007.10.008","article-title":"Dermal sensitization quantitative risk assessment (QRA) for fragrance ingredients","volume":"52","author":"Api","year":"2008","journal-title":"Regulatory Toxicology and Pharmacology"},{"key":"10.1016\/j.tiv.2009.10.015_bib3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0278-6915(01)00130-2","article-title":"Local lymph node assay \u2013 validation, conduct and use in practice","volume":"40","author":"Basketter","year":"2002","journal-title":"Food and Chemical Toxicology"},{"key":"10.1016\/j.tiv.2009.10.015_bib4","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1600-0536.2006.00930.x","article-title":"Identification and classification of skin sensitizers: identifying false positives and false negatives","volume":"55","author":"Basketter","year":"2006","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2009.10.015_bib5","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1600-0536.2008.01444.x","article-title":"Nothing is perfect, not even the local lymph node assay: a commentary and the implications for REACH","volume":"60","author":"Basketter","year":"2009","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2009.10.015_bib6","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1177\/026119290503300403","article-title":"The in vitro skin irritation of chemicals: optimisation of the EPISKIN prediction model within the framework of the ECVAM validation process","volume":"33","author":"Cotovio","year":"2005","journal-title":"ATLA Alternatives to Laboratory Animals"},{"key":"10.1016\/j.tiv.2009.10.015_bib7","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1093\/toxsci\/kfh213","article-title":"Development of a peptide reactivity assay for screening contact allergens","volume":"81","author":"Gerberick","year":"2004","journal-title":"Toxicological Sciences"},{"key":"10.1016\/j.tiv.2009.10.015_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1177\/026119290803600210","article-title":"Chemical reactivity measurement and the predictive identification of skin sensitisers","volume":"36","author":"Gerberick","year":"2008","journal-title":"ATLA Alternatives to Laboratory Animals"},{"key":"10.1016\/j.tiv.2009.10.015_bib9","article-title":"Conception, characterization and correlation of new marine odorants","volume":"7","author":"Kraft","year":"2003","journal-title":"European Journal of Organic Chemistry"},{"key":"10.1016\/j.tiv.2009.10.015_bib10","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1093\/toxsci\/kfm259","article-title":"Skin sensitizers induce antioxidant response element dependent genes: application to the in vitro testing of the sensitization potential of chemicals","volume":"102","author":"Natsch","year":"2008","journal-title":"Toxicological Sciences"},{"key":"10.1016\/j.tiv.2009.10.015_bib11","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1093\/toxsci\/kfn194","article-title":"LC\u2013MS-based characterization of the peptide reactivity of chemicals to improve the in vitro prediction of the skin sensitization potential","volume":"106","author":"Natsch","year":"2008","journal-title":"Toxicological Sciences"},{"key":"10.1016\/j.tiv.2009.10.015_bib12","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.tiv.2007.03.016","article-title":"Utility and limitations of a peptide reactivity assay to predict fragrance allergens in vitro","volume":"21","author":"Natsch","year":"2007","journal-title":"Toxicology in Vitro"},{"key":"10.1016\/j.tiv.2009.10.015_bib13","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.tiv.2005.10.014","article-title":"Development of an in vitro skin sensitization test using human cell lines; human cell line activation test (h-CLAT) II. An inter-laboratory study of the h-CLAT","volume":"20","author":"Sakaguchi","year":"2006","journal-title":"Toxicology in Vitro"},{"key":"10.1016\/j.tiv.2009.10.015_bib14","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(81)90240-2","article-title":"Specific colorimetric detection of o-diphenols and 3,4-dihydroxyphenylalanine-containing peptides","volume":"111","author":"Waite","year":"1981","journal-title":"Analytical Biochemistry"},{"key":"10.1016\/j.tiv.2009.10.015_bib15","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1124\/dmd.104.002840","article-title":"Identification of a novel glutathione adduct of diclofenac, 4\u2032-hydroxy-2\u2032-glutathion-deschloro-diclofenac, upon incubation with human liver microsomes","volume":"33","author":"Yu","year":"2005","journal-title":"Drug Metabolism and Disposition"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233309003245?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233309003245?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:29:58Z","timestamp":1760135398000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233309003245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["S0887233309003245"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2009.10.015","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Use of in vitro testing to identify an unexpected skin sensitizing impurity in a commercial product: A case study","name":"articletitle","label":"Article Title"},{"value":"Toxicology in Vitro","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tiv.2009.10.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}