{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:56:53Z","timestamp":1791331013101,"version":"4.3.1"},"reference-count":50,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1016\/j.tiv.2012.12.025","type":"journal-article","created":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T18:23:05Z","timestamp":1356978185000},"page":"857-863","source":"Crossref","is-referenced-by-count":21,"title":["Development of an in vitro skin sensitization test based on ROS production in THP-1 cells"],"prefix":"10.1016","volume":"27","author":[{"given":"Kazutoshi","family":"Saito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Miyazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Nukada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Sakaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Nishiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2012.12.025_b0005","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/jid.2008.209","article-title":"Skin sensitization to p-phenylenediamine: the diverging roles of oxidation and N-acetylation for dendritic cell activation and the immune response","volume":"129","author":"Aeby","year":"2009","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.tiv.2012.12.025_b0010","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/j.tiv.2010.07.005","article-title":"Identifying and characterizing chemical skin sensitizers without animal testing: Colipa\u2019s research and method development program","volume":"24","author":"Aeby","year":"2010","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tiv.2012.12.025_b0015","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1080\/10715760601134459","article-title":"Reactive oxygen and nitrogen species are involved in sorbitol-induced apoptosis of human erithroleukaemia cells K562","volume":"41","author":"Aquilano","year":"2007","journal-title":"Free Radic. Res."},{"key":"10.1016\/j.tiv.2012.12.025_b0020","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1177\/026119291003800403","article-title":"A comparative evaluation of in vitro skin sensitisation tests: the human cell-line activation test (h-CLAT) versus the local lymph node assay (LLNA)","volume":"38","author":"Ashikaga","year":"2010","journal-title":"Altern. Lab. Anim."},{"key":"10.1016\/j.tiv.2012.12.025_b0025","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.taap.2005.06.018","article-title":"The cytokine-dependent MUTZ-3 cell line as an in vitro model for the screening of contact sensitizers","volume":"212","author":"Azam","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0030","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1034\/j.1600-0536.2000.042006344.x","article-title":"Use of the local lymph node assay for the estimation of relative contact allergenic potency","volume":"42","author":"Basketter","year":"2000","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2012.12.025_b0035","doi-asserted-by":"crossref","first-page":"269","DOI":"10.5021\/ad.2010.22.3.269","article-title":"The roles of reactive oxygen species produced by contact allergens and irritants in monocyte-derived dendritic cells","volume":"22","author":"Byamba","year":"2010","journal-title":"Ann. Dermatol."},{"key":"10.1016\/j.tiv.2012.12.025_b0040","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1177\/026119290903700313","article-title":"Selection of chemicals for the development and evaluation of in vitro methods for skin sensitisation testing","volume":"37","author":"Casati","year":"2009","journal-title":"Altern. Lab. Anim."},{"key":"10.1016\/j.tiv.2012.12.025_b0045","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1002\/mnfr.200600230","article-title":"The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells","volume":"51","author":"Chen","year":"2007","journal-title":"Mol. Nutr. Food Res."},{"key":"10.1016\/j.tiv.2012.12.025_b0050","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.freeradbiomed.2009.10.028","article-title":"Cinnamaldehydes inhibit thioredoxin reductase and induce Nrf2: potential candidates for cancer therapy and chemoprevention","volume":"48","author":"Chew","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.tiv.2012.12.025_b0055","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.freeradbiomed.2011.06.033","article-title":"Cross talk between mitochondria and NADPH oxidases","volume":"51","author":"Dikalov","year":"2011","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.tiv.2012.12.025_b0060","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.0105-1873.2005.00683.x","article-title":"Hapten-protein binding: from theory to practical application in the in vitro prediction of skin sensitization","volume":"53","author":"Divkovic","year":"2005","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2012.12.025_b0065","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.taap.2010.03.009","article-title":"Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro","volume":"245","author":"Emter","year":"2010","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0070","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.2741\/3303","article-title":"Crosstalk signaling between mitochondrial Ca2+ and ROS","volume":"14","author":"Feissner","year":"2009","journal-title":"Front Biosci."},{"key":"10.1016\/j.tiv.2012.12.025_b0075","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/j.1600-0536.1995.tb02054.x","article-title":"Contact sensitivity to vanilla in a lip salve","volume":"33","author":"Ferguson","year":"1995","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2012.12.025_b0080","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/0272-0590(92)90183-I","article-title":"Examination of the local lymph node assay for use in contact sensitization risk assessment","volume":"19","author":"Gerberick","year":"1992","journal-title":"Fundam. Appl. Toxicol."},{"key":"10.1016\/j.tiv.2012.12.025_b0085","first-page":"157","article-title":"Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods","volume":"16","author":"Gerberick","year":"2005","journal-title":"Dermatitis"},{"key":"10.1016\/j.tiv.2012.12.025_b0090","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1743-7075-3-30","article-title":"Modulation of adipocyte lipogenesis by octanoate: involvement of reactive oxygen species","volume":"3","author":"Guo","year":"2006","journal-title":"Nutr. Metab. (London)"},{"key":"10.1016\/j.tiv.2012.12.025_b0095","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.taap.2008.03.014","article-title":"A cell-based in vitro alternative to identify skin sensitizers by gene expression","volume":"231","author":"Hooyberghs","year":"2008","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0100","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1021\/tx049688+","article-title":"Selective haptenation of cellular or extracellular protein by chemical allergens: association with cytokine polarization","volume":"18","author":"Hopkins","year":"2005","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.tiv.2012.12.025_b0105","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1021\/tx7002239","article-title":"Allergic contact dermatitis \u2013 formation, structural requirements, and reactivity of skin sensitizers","volume":"21","author":"Karlberg","year":"2008","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.tiv.2012.12.025_b0110","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.3390\/ijms11103769","article-title":"Reactive oxygenspecies enhance TLR10expressionin thehumanmonocyticcelllineTHP-1","volume":"11","author":"Kim","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0115","first-page":"567","article-title":"Assessment of the skin sensitization potential of topical medicaments using the local lymph node assay: an interlaboratory evaluation","volume":"53","author":"Kimber","year":"1998","journal-title":"J. Toxicol. Environ. Health A"},{"key":"10.1016\/j.tiv.2012.12.025_b0120","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1046\/j.1365-2133.2000.03349.x","article-title":"Cytokines and chemokines in the initiation and regulation of epidermal Langerhans cell mobilization","volume":"142","author":"Kimber","year":"2000","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.tiv.2012.12.025_b0125","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1034\/j.1600-0536.2002.470601.x","article-title":"The local lymph node assay: past, present and future","volume":"47","author":"Kimber","year":"2002","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2012.12.025_b0130","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.taap.2009.01.026","article-title":"THP-1 monocytes but not macrophages as a potential alternative for CD34+ dendritic cells to identify chemical skin sensitisers","volume":"236","author":"Lambrechts","year":"2009","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0135","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1093\/toxsci\/kfq108","article-title":"Assessment of chemical skin-sensitizing potency by an in vitro assay based on human dendritic cells","volume":"116","author":"Lambrechts","year":"2010","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0140","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.yrtph.2009.08.016","article-title":"Potency values from the local lymph node assay: application to classification, labelling and risk assessment","volume":"56","author":"Loveless","year":"2010","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0145","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tox.2010.04.016","article-title":"Sensitization effect of thimerosal is mediated in vitro via reactive oxygen species and calcium signaling","volume":"274","author":"Migdal","year":"2010","journal-title":"Toxicology"},{"key":"10.1016\/j.tiv.2012.12.025_b0150","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.taap.2010.04.007","article-title":"Responsiveness of human monocyte-derived dendritic cells to thimerosal and mercury derivatives","volume":"246","author":"Migdal","year":"2010","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0155","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.tiv.2007.10.005","article-title":"Role of p38 MAPK in the selective release of IL-8 induced by chemical allergen in naive THP-1 cells","volume":"22","author":"Mitjans","year":"2008","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tiv.2012.12.025_b0160","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1016\/j.tiv.2010.06.001","article-title":"Use of IL-8 release and p38 MAPK activation in THP-1 cells to identify allergens and to assess their potency in vitro","volume":"24","author":"Mitjans","year":"2010","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tiv.2012.12.025_b0165","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.tiv.2006.10.005","article-title":"Phenotypic alterations and cytokine production in THP-1 cells in response to allergens","volume":"21","author":"Miyazawa","year":"2007","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tiv.2012.12.025_b0170","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1038\/sj.jid.5700802","article-title":"EC-SOD suppresses contact hypersensitivity in mouse skin by impairing Langerhans cell migration","volume":"127","author":"Na","year":"2007","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.tiv.2012.12.025_b0175","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1093\/toxsci\/kfm259","article-title":"Skin sensitizers induce antioxidant response element dependent genes: application to the in vitro testing of the sensitization potential of chemicals","volume":"102","author":"Natsch","year":"2008","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0180","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1021\/tx1002707","article-title":"Chemical basis for the extreme skins sensitization potency of (E)-4-(ethoxymethylene)-2-phenyloxazol-5(4H)-one","volume":"23","author":"Natsch","year":"2010","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.tiv.2012.12.025_b0185","doi-asserted-by":"crossref","first-page":"10835","DOI":"10.1074\/jbc.273.18.10835","article-title":"Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue","volume":"273","author":"Nordberg","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.tiv.2012.12.025_b0190","doi-asserted-by":"crossref","first-page":"175","DOI":"10.2131\/jts.33.175","article-title":"Production of IL-8 in THP-1 cells following contact allergen stimulation via mitogen-activated protein kinase activation or tumor necrosis factor-alpha production","volume":"33","author":"Nukada","year":"2008","journal-title":"J. Toxicol. Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0195","doi-asserted-by":"crossref","first-page":"313","DOI":"10.2131\/jts.36.313","article-title":"The relationship between CD86 and CD54 protein expression and cytotoxicity following stimulation with contact allergen in THP-1 cells","volume":"36","author":"Nukada","year":"2011","journal-title":"J. Toxicol. Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0200","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00018-011-0846-8","article-title":"Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects. Current knowledge assembled at an international workshop at BfR, Germany","volume":"69","author":"Peiser","year":"2012","journal-title":"Cell Mol. Life Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0205","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.tem.2009.04.001","article-title":"Mitochondrialgenerationoffree radicals and hypoxic signaling","volume":"20","author":"Poyton","year":"2009","journal-title":"Trends Endocrinol. Metab."},{"key":"10.1016\/j.tiv.2012.12.025_b0210","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.taap.2006.12.026","article-title":"Assessment of the U937 cell line for the detection of contact allergens","volume":"220","author":"Python","year":"2007","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0215","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s10565-008-9059-9","article-title":"The relationship between CD86\/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test-human cell line activation test (h-CLAT)","volume":"25","author":"Sakaguchi","year":"2009","journal-title":"Cell Biol. Toxicol."},{"key":"10.1016\/j.tiv.2012.12.025_b0220","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s12016-007-0034-7","article-title":"Dendritic cells and contact dermatitis","volume":"33","author":"Sasaki","year":"2007","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"10.1016\/j.tiv.2012.12.025_b0225","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.yrtph.2004.02.002","article-title":"Quantitative relationship between the local lymph node assay and human skin sensitization assays","volume":"39","author":"Schneider","year":"2004","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.tiv.2012.12.025_b0230","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.tiv.2005.06.039","article-title":"CXCL8 secretion by dendritic cells predicts contact allergens from irritants","volume":"20","author":"Toebak","year":"2006","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tiv.2012.12.025_b0235","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1600-0536.2008.01443.x","article-title":"Dendritic cells: biology of the skin","volume":"60","author":"Toebak","year":"2009","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2012.12.025_b0240","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1248\/jhs.47.327","article-title":"Skin reaction induced by aldehydes for food flavoring agents","volume":"47","author":"Watanabe","year":"2001","journal-title":"J. Health Sci."},{"key":"10.1016\/j.tiv.2012.12.025_b0245","doi-asserted-by":"crossref","first-page":"25096","DOI":"10.1074\/jbc.M007383200","article-title":"Leptin induces mitochondrial superoxide production and monocyte chemoattractant protein-1 expression in aortic endothelial cells by increasing fatty acid oxidation via protein kinase A","volume":"276","author":"Yamagishi","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.tiv.2012.12.025_b0250","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0887-2333(03)00006-7","article-title":"Evaluation of the skin sensitization potential of chemicals using expression of co-stimulatory molecules, CD54 and CD86, on the naive THP-1 cell line","volume":"17","author":"Yoshida","year":"2003","journal-title":"Toxicol. In Vitro"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233312003633?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233312003633?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,19]],"date-time":"2020-07-19T15:47:31Z","timestamp":1595173651000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233312003633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["S0887233312003633"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2012.12.025","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}