{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:34:34Z","timestamp":1789929274385,"version":"4.0.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.tiv.2019.04.014","type":"journal-article","created":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T02:16:33Z","timestamp":1555467393000},"page":"161-178","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["A newly developed means of HPLC-fluorescence analysis for predicting the skin sensitization potential of multi-constituent substances using ADRA"],"prefix":"10.1016","volume":"59","author":[{"given":"Masaharu","family":"Fujita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Wanibuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Katsuoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Kasahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2019.04.014_bb0005","doi-asserted-by":"crossref","first-page":"56","DOI":"10.5539\/jfr.v4n3p56","article-title":"Determination of the chemical composition of tea by chromatographic methods: a review","volume":"4","author":"Alexandr","year":"2015","journal-title":"J. Food Res."},{"key":"10.1016\/j.tiv.2019.04.014_bb0010","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.tiv.2013.02.008","article-title":"The sensitivity of the KeratinoSens\u2122 assay to evaluate plant extracts: a pilot study","volume":"27","author":"Andres","year":"2013","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.tiv.2019.04.014_bb0015","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.taap.2015.09.027","article-title":"A fluorescence high throughput screening method for the detection of reactive electrophiles as potential skin sensitizers","volume":"289","author":"Avonto","year":"2015","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tiv.2019.04.014_bb0020","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.tiv.2017.09.007","article-title":"Chemical stability and in chemico reactivity of 24 fragrance ingredients of concern for skin sensitization risk assessment","volume":"46","author":"Avonto","year":"2018","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.tiv.2019.04.014_bb0025","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S0021-9673(01)96244-2","article-title":"Fluorescence detection in liquid chromatography with an intensified diode-array detector","volume":"556","author":"Cecil","year":"1991","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.tiv.2019.04.014_bb0030","doi-asserted-by":"crossref","first-page":"13027","DOI":"10.3390\/molecules190913027","article-title":"Structures of new phenolics isolated from Licorice, and the effectiveness of Licorice phenolics on Vancomycin-Resistant Enterococci","volume":"19","author":"Eerdunbayaer","year":"2014","journal-title":"Molecules"},{"key":"10.1016\/j.tiv.2019.04.014_bb0035","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1021\/tx0497806","article-title":"Structure-activity models for contact sensitization","volume":"18","author":"Fedorowicz","year":"2005","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.tiv.2019.04.014_bb0040","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1039\/a909219f","article-title":"HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas","volume":"125","author":"Fernandez","year":"2000","journal-title":"Analysit"},{"key":"10.1016\/j.tiv.2019.04.014_bb0045","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.1021\/ja01094a025","article-title":"Relative nucleophilic reactivities of amino groups and mercaptide ions in addition reactions with \u03b1,\u03b2-unsaturated compounds","volume":"87","author":"Friedman","year":"1965","journal-title":"J. Am. Chem. Soc."},{"issue":"1","key":"10.1016\/j.tiv.2019.04.014_bb0050","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.vascn.2014.06.001","article-title":"Development of a prediction method for skin sensitization using novel cysteine and lysine derivatives","volume":"70","author":"Fujita","year":"2014","journal-title":"J. Pharmacol. Toxicol. Methods"},{"issue":"2","key":"10.1016\/j.tiv.2019.04.014_bb0055","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/jat.3707","article-title":"The cause of and countermeasures for oxidation of the cysteine-derived reagent used in the amino acid derivative reactivity assay","volume":"39","author":"Fujita","year":"2019","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.tiv.2019.04.014_bb0060","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1093\/toxsci\/kfh213","article-title":"Development of a peptide reactivity assay for screening contact allergens","volume":"81","author":"Gerberick","year":"2004","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tiv.2019.04.014_bb0065","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/toxsci\/kfm064","article-title":"Quantification of chemical peptide reactivity for screening contact allergens: a classification tree model approach","volume":"97","author":"Gerberick","year":"2007","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tiv.2019.04.014_bb0070","first-page":"334","article-title":"Green tea composition, consumption, and polyphenol chemistry","volume":"21","author":"Graham","year":"1992","journal-title":"PreventiveMedicine"},{"key":"10.1016\/j.tiv.2019.04.014_bb0075","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.tox.2013.10.006","article-title":"Cintact sensitizing potential of pyrogallol and 5-amino-o-cresol in female BALB\/c mice","volume":"314","author":"Guo","year":"2013","journal-title":"Toxicology"},{"key":"10.1016\/j.tiv.2019.04.014_bb0080","series-title":"Hagers Handbuch der Pharmazeutischen Praxis. Monograph: Valeriana","author":"Hansel","year":"1994"},{"key":"10.1016\/j.tiv.2019.04.014_bb0085","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1248\/cpb.48.1286","article-title":"Phenolic constituents Licorice. VII. Structures of glicophenone and Glicoisoflavone, and effects of Licorice phenolics on methicillin-resistant Staphylococcus","volume":"48","author":"Hatano","year":"2000","journal-title":"Chem. Pharm. Bull."},{"key":"10.1016\/j.tiv.2019.04.014_bb0090","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/S0003-2670(01)93016-8","article-title":"Fluorimetric determination of secondary amino acids by 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole","volume":"130","author":"Imai","year":"1981","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.tiv.2019.04.014_bb0095","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.fct.2005.11.003","article-title":"Safety studies on epigallocatechin gallate (EGCG) preparations. Part 2: dermal, acute and short-term toxicity studies","volume":"44","author":"Isbrucher","year":"2006","journal-title":"Food Chem. Toxicol."},{"issue":"9","key":"10.1016\/j.tiv.2019.04.014_bb0100","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1021\/ed043p469","article-title":"The fates of electronic excitation energy","volume":"43","author":"Jaffe","year":"1966","journal-title":"J. Chem. Educ."},{"issue":"2","key":"10.1016\/j.tiv.2019.04.014_bb0105","first-page":"209","article-title":"Irritant contact dermatitis from Aloe Arborescens Miller","volume":"29","author":"Kubo","year":"1987","journal-title":"Skin Res."},{"key":"10.1016\/j.tiv.2019.04.014_bb0110","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s13104-017-2385-3","article-title":"Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidatnt activity of Aloe vera (L.) Burm.f","volume":"10","author":"Kumar","year":"2017","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.tiv.2019.04.014_bb0115","doi-asserted-by":"crossref","first-page":"6891","DOI":"10.1021\/jf071110t","article-title":"Aloe ferox leaf gel phytochemical content, antioxidant capacity,and possible health benefits","volume":"55","author":"Loots","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.tiv.2019.04.014_bb0120","doi-asserted-by":"crossref","first-page":"5108","DOI":"10.3390\/molecules17055108","article-title":"Absorption and fluorescence spectroscopic properties of 1- and 1,4-Silyl-substituted napthalene derivatives","volume":"17","author":"Maeda","year":"2012","journal-title":"Molecules"},{"issue":"2\u20133","key":"10.1016\/j.tiv.2019.04.014_bb0125","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s10616-007-9112-1","article-title":"In vitro and in vivo anti-allergic effects of 'benifuuki' green tea containing O-methylated catechin and ginger extract enhancement","volume":"55","author":"Maeda-Yamamoto","year":"2007","journal-title":"Cytotechnology"},{"key":"10.1016\/j.tiv.2019.04.014_bb0130","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jchromb.2008.05.012","article-title":"Development and validation of HPLC method for the determination of tobramycin in urine samples post-inhalation using pre-column derivatisation with fluorescein isothiocyanate","volume":"869","author":"Mashat","year":"2008","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.tiv.2019.04.014_bb0135","doi-asserted-by":"crossref","first-page":"817","DOI":"10.2331\/suisan.51.817","article-title":"Analyses of dansyl and dabsyl amino acids by reverse \u2013phase high-performance liquid chromatography","volume":"51","author":"Muramoto","year":"1985","journal-title":"Bull. Jpn. Soc. Sci. Fish."},{"key":"10.1016\/j.tiv.2019.04.014_bb0140","doi-asserted-by":"crossref","first-page":"5639","DOI":"10.1021\/j100073a011","article-title":"The influence of planarity and rigidity on the absorption and fluorescence parameters and intersystem crossing rate constant in aromatic molecules","volume":"98","author":"Nijegorodov","year":"1994","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.tiv.2019.04.014_bb0145","series-title":"The Local Lymph Node Assay (LLNA)","article-title":"OECD guideline for the testing of chemicals no. 429","author":"OECD","year":"2010"},{"key":"10.1016\/j.tiv.2019.04.014_bb0150","series-title":"Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA)","article-title":"OECD guideline for the testing of chemicals 442C","author":"OECD","year":"2015"},{"key":"10.1016\/j.tiv.2019.04.014_bb0155","series-title":"OECD GUIDELINE FOR THE TESTING OF CHEMICALS 442D, Appendix IA: In Vitro Skin Sensitisation: The ARE-Nrf2 Luciferase KeratinoSens\u2122 Test Method","author":"OECD","year":"2018"},{"key":"10.1016\/j.tiv.2019.04.014_bb0160","series-title":"OECD GUIDELINE FOR THE TESTING OF CHEMICALS 442D, Appendix IB: In Vitro Skin Sensitisation: The ARE-Nrf2 Luciferase LuSens Test Method","author":"OECD","year":"2018"},{"key":"10.1016\/j.tiv.2019.04.014_bb0165","series-title":"OECD Guideline for the Testing of Chemicals 442E, Annex I: In Vitro Skin Sensitisation: Human Cell Line Activation Test (h-CLAT)","author":"OECD","year":"2018"},{"key":"10.1016\/j.tiv.2019.04.014_bb0170","series-title":"OECD Guideline for the Testing of Chemicals 442E, Annex Ii: In Vitro Skin Sensitisation: U937 cell Line Activation Test (u-SENS\u2122)","author":"OECD","year":"2018"},{"key":"10.1016\/j.tiv.2019.04.014_bb0175","series-title":"OECD Guideline for the Testing of Chemicals 442E, Annex iii: In Vitro Skin Sensitisation: Il-8 Luc Assay","author":"OECD","year":"2018"},{"key":"10.1016\/j.tiv.2019.04.014_bb0180","first-page":"1251","article-title":"New method for determination of free amino groups in intact pure proteins: relationship to available lysine","volume":"59","author":"Purcell","year":"1976","journal-title":"J. Assoc. Off. Anal. Chem."},{"key":"10.1016\/j.tiv.2019.04.014_bb0185","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1039\/b102541b","article-title":"Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection","volume":"126","author":"Sano","year":"2001","journal-title":"Analyst"},{"key":"10.1016\/j.tiv.2019.04.014_bb0190","first-page":"1","article-title":"Extraction, isolation and characterization of bioactive compounds from plants' extracts","volume":"8","author":"Sasidharan","year":"2011","journal-title":"Afr. J. Tradit. Complement. Altern. Med."},{"key":"10.1016\/j.tiv.2019.04.014_bb0195","series-title":"Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules","author":"Sauer","year":"2011"},{"key":"10.1016\/j.tiv.2019.04.014_bb0200","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1021\/ac60367a046","article-title":"Fluoroscence measurements of benzene, naphthalene, anthracene, pyrene, fluoranthene, and benzo[e]pyrene in water","volume":"48","author":"Schwarz","year":"1976","journal-title":"Anal. Chem."},{"issue":"3","key":"10.1016\/j.tiv.2019.04.014_bb0205","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1055\/s-2007-967126","article-title":"In vitro and in vivo antiallergic effects of Glycyrrhiza glabra and its components","volume":"73","author":"Shin","year":"2007","journal-title":"Planta Med."},{"key":"10.1016\/j.tiv.2019.04.014_bb0210","series-title":"Aloe Vera: A Short Review","author":"Surjushe","year":"2008"},{"key":"10.1016\/j.tiv.2019.04.014_bb0215","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0003-2697(81)90534-0","article-title":"Dansylation of amino acids for high-performance liquid chromatography analysis","volume":"115","author":"Tapuhi","year":"1981","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.tiv.2019.04.014_bb0220","first-page":"850","article-title":"Difference of solvent polarity to phytochemical content and antioxidant activity of pluchea indicia less leaves extracts","volume":"6","author":"Widyawati","year":"2014","journal-title":"Int. J. Pharm. Phytochem. Res."},{"issue":"11","key":"10.1016\/j.tiv.2019.04.014_bb0225","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1002\/jat.3139","article-title":"A novel in chemico method to detect skin sensitizers in highly diluted reaction conditions","volume":"35","author":"Yamamoto","year":"2015","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.tiv.2019.04.014_bb0230","author":"Yamamoto","year":"2019","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"10.1016\/j.tiv.2019.04.014_bb0235","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1021\/ol503104b","article-title":"Asymmetric oxy-Michael addition to \u03b3-hydroxy-\u03b1,\u03b2-unsaturated carbonyls using formaldehyde as an oxygen-centered nucleophile","volume":"16","author":"Yoneda","year":"2014","journal-title":"Org. Lett."},{"key":"10.1016\/j.tiv.2019.04.014_bb0240","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jchromb.2017.04.003","article-title":"High sensitivity HPLC method for analysis of in vivo extracellular GABA using optimized fluorescence parameters for o-phthalaldehyde (OPA)\/sulfite derivatives","volume":"1055-1056","author":"Zandy","year":"2017","journal-title":"J. Chromatogr. B"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233318306040?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233318306040?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,7,5]],"date-time":"2019-07-05T02:15:17Z","timestamp":1562292917000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233318306040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":48,"alternative-id":["S0887233318306040"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2019.04.014","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A newly developed means of HPLC-fluorescence analysis for predicting the skin sensitization potential of multi-constituent substances using ADRA","name":"articletitle","label":"Article Title"},{"value":"Toxicology in Vitro","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tiv.2019.04.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}