{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:14:16Z","timestamp":1792808056062,"version":"4.5.0"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T00:00:00Z","timestamp":1571184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000008","name":"Health Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000008","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.tiv.2019.104662","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T19:57:28Z","timestamp":1571255848000},"page":"104662","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"special_numbering":"C","title":["A rapid assay of human thyroid peroxidase activity"],"prefix":"10.1016","volume":"62","author":[{"given":"Hongyan","family":"Dong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlena","family":"Godlewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Wade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.tiv.2019.104662_bb0005","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1089\/thy.2008.0341","article-title":"Delayed neurobehavioral development in children born to pregnant women with mild hypothyroxinemia during the first month of gestation: the importance of early iodine supplementation","volume":"19","author":"Berbel","year":"2009","journal-title":"Thyroid"},{"issue":"10","key":"10.1016\/j.tiv.2019.104662_bb0010","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1172\/JCI29812","article-title":"Deiodinases: implications of the local control of thyroid hormone action","volume":"116","author":"Bianco","year":"2006","journal-title":"J. Clin. Invest."},{"issue":"3","key":"10.1016\/j.tiv.2019.104662_bb0015","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0041-008X(86)90337-6","article-title":"Binding of a metabolite of 3,4,3\u2032,4\u2032-tetrachlorobiphenyl to transthyretin reduces serum vitamin A transport by inhibiting the formation of the protein complex carrying both retinol and thyroxin","volume":"85","author":"Brouwer","year":"1986","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0020","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1006\/taap.1999.8826","article-title":"Assessing the role of ortho-substitution on polychlorinated biphenyl binding to transthyretin, a thyroxine transport protein","volume":"162","author":"Chauhan","year":"2000","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"2","key":"10.1016\/j.tiv.2019.104662_bb0025","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1021\/tx00038a008","article-title":"Inhibition of peroxidase-catalyzed reactions by arylamines: mechanism for the anti-thyroid action of sulfamethazine","volume":"7","author":"Doerge","year":"1994","journal-title":"Chem. Res. Toxicol."},{"issue":"2","key":"10.1016\/j.tiv.2019.104662_bb0030","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1021\/tx00014a003","article-title":"Mechanism of thyroid peroxidase inhibition by ethylenethiourea","volume":"3","author":"Doerge","year":"1990","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.tiv.2019.104662_bb0035","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.tiv.2017.01.014","article-title":"Application of a nonradioactive assay for high throughput screening for inhibition of thyroid hormone uptake via the transmembrane transporter MCT8","volume":"40","author":"Dong","year":"2017","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.tiv.2019.104662_bb0040","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.tiv.2019.01.021","article-title":"Development of a non-radioactive screening assay to detect chemicals disrupting the human sodium iodide symporter activity","volume":"57","author":"Dong","year":"2019","journal-title":"Toxicol. in Vitro"},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0045","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.tiv.2010.08.013","article-title":"Detection of thyroid hormone receptor disruptors by a novel stable in vitro reporter gene assay","volume":"25","author":"Freitas","year":"2011","journal-title":"Toxicol. in Vitro"},{"issue":"2\u20133","key":"10.1016\/j.tiv.2019.104662_bb0050","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.tox.2005.09.005","article-title":"Studies on the goitrogenic mechanism of action of N,N,N',N'-tetramethylthiourea","volume":"217","author":"Freyberger","year":"2006","journal-title":"Toxicology"},{"issue":"2","key":"10.1016\/j.tiv.2019.104662_bb0055","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1089\/thy.2013.0127","article-title":"A redundant role of human thyroid peroxidase propeptide for cellular, enzymatic, and immunological activity","volume":"24","author":"Godlewska","year":"2014","journal-title":"Thyroid"},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0060","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/j.1365-2249.2004.02422.x","article-title":"Evaluation of conformational epitopes on thyroid peroxidase by antipeptide antibody binding and mutagenesis","volume":"136","author":"Gora","year":"2004","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.tiv.2019.104662_bb0065","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.tiv.2016.12.006","article-title":"Development of a screening approach to detect thyroid disrupting chemicals that inhibit the human sodium iodide symporter (NIS)","volume":"40","author":"Hallinger","year":"2017","journal-title":"Toxicol. in Vitro"},{"issue":"2","key":"10.1016\/j.tiv.2019.104662_bb0070","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1093\/toxsci\/kfx279","article-title":"Screening the ToxCast phase 1 chemical library for inhibition of deiodinase type 1 activity","volume":"162","author":"Hornung","year":"2018","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tiv.2019.104662_bb0075","series-title":"Concise International Chemical Assessment Document 71","author":"IPCS","year":"2006"},{"key":"10.1016\/j.tiv.2019.104662_bb0080","series-title":"Assay Guidance Manual","article-title":"HTS assay validation","author":"Iversen","year":"2004"},{"issue":"3","key":"10.1016\/j.tiv.2019.104662_bb0085","doi-asserted-by":"crossref","first-page":"331","DOI":"10.14573\/altex.2013.3.331","article-title":"In vitro metabolism and bioavailability tests for endocrine active substances: what is needed next for regulatory purposes?","volume":"30","author":"Jacobs","year":"2013","journal-title":"ALTEX"},{"issue":"3","key":"10.1016\/j.tiv.2019.104662_bb0090","first-page":"191","article-title":"Simple and rapid in vitro assay for detecting human thyroid peroxidase disruption","volume":"32","author":"Jomaa","year":"2015","journal-title":"ALTEX"},{"issue":"3","key":"10.1016\/j.tiv.2019.104662_bb0095","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/0272-0590(87)90023-6","article-title":"Comparative metabolism and excretion of resorcinol in male and female F344 rats","volume":"9","author":"Kim","year":"1987","journal-title":"Fundam. Appl. Toxicol."},{"issue":"6","key":"10.1016\/j.tiv.2019.104662_bb0100","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1111\/j.1365-2265.2009.03743.x","article-title":"Abnormalities of maternal thyroid function during pregnancy affect neuropsychological development of their children at 25-30 months","volume":"72","author":"Li","year":"2010","journal-title":"Clin. Endocrinol."},{"issue":"4","key":"10.1016\/j.tiv.2019.104662_bb0105","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1080\/15287399209531686","article-title":"Antithyroid effects of coal-derived pollutants","volume":"37","author":"Lindsay","year":"1992","journal-title":"J. Toxicol. Environ. Health"},{"key":"10.1016\/j.tiv.2019.104662_bb0110","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.mce.2017.02.038","article-title":"Evolution of thyroid hormone distributor proteins","volume":"459","author":"McLean","year":"2017","journal-title":"Mol. Cell. Endocrinol."},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0115","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1093\/toxsci\/56.1.95","article-title":"Potent competitive interactions of some brominated flame retardants and related compounds with human transthyretin in vitro","volume":"56","author":"Meerts","year":"2000","journal-title":"Toxicol. Sci."},{"issue":"3","key":"10.1016\/j.tiv.2019.104662_bb0120","first-page":"367","article-title":"Pharmacokinetics of resorcinol in the rat","volume":"38","author":"Merker","year":"1982","journal-title":"Res. Commun. Chem. Pathol. Pharmacol."},{"issue":"9","key":"10.1016\/j.tiv.2019.104662_bb0125","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1001\/jamapediatrics.2015.0498","article-title":"Maternal mild thyroid hormone insufficiency in early pregnancy and attention-deficit\/hyperactivity disorder symptoms in children","volume":"169","author":"Modesto","year":"2015","journal-title":"JAMA Pediatr."},{"key":"10.1016\/j.tiv.2019.104662_bb0130","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.yrtph.2016.06.019","article-title":"A comparison of potency differences among thyroid peroxidase (TPO) inhibitors to induce developmental toxicity and other thyroid gland-linked toxicities in humans and rats","volume":"80","author":"Motonaga","year":"2016","journal-title":"Regul. Toxicol. Pharmacol."},{"issue":"4","key":"10.1016\/j.tiv.2019.104662_bb0135","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1016\/j.tiv.2013.02.012","article-title":"Mechanism-based testing strategy using in vitro approaches for identification of thyroid hormone disrupting chemicals","volume":"27","author":"Murk","year":"2013","journal-title":"Toxicol. in Vitro"},{"issue":"1\u20132","key":"10.1016\/j.tiv.2019.104662_bb0140","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0009-8981(78)90216-4","article-title":"Improved assay procedures for thyroid peroxidase; application to normal and adenomatous human thyroid tissue","volume":"83","author":"Nakashima","year":"1978","journal-title":"Clin. Chim. Acta"},{"issue":"2","key":"10.1016\/j.tiv.2019.104662_bb0145","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1093\/toxsci\/kfy302","article-title":"Screening the ToxCast phase 1, phase 2, and e1k chemical libraries for inhibitors of iodothyronine deiodinases","volume":"168","author":"Olker","year":"2019","journal-title":"Toxicol. Sci."},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0150","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1093\/toxsci\/kfw034","article-title":"Tiered high-throughput screening approach to identify thyroperoxidase inhibitors within the ToxCast phase I and II chemical libraries","volume":"151","author":"Paul Friedman","year":"2016","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tiv.2019.104662_bb0155","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.tox.2013.08.006","article-title":"Cross-species analysis of thyroperoxidase inhibition by xenobiotics demonstrates conservation of response between pig and rat","volume":"312","author":"Paul","year":"2013","journal-title":"Toxicology"},{"issue":"3","key":"10.1016\/j.tiv.2019.104662_bb0160","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1021\/tx400310w","article-title":"Development of a thyroperoxidase inhibition assay for high-throughput screening","volume":"27","author":"Paul","year":"2014","journal-title":"Chem. Res. Toxicol."},{"issue":"8","key":"10.1016\/j.tiv.2019.104662_bb0165","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1089\/thy.2015.0058","article-title":"An improved nonradioactive screening method identifies genistein and xanthohumol as potent inhibitors of iodothyronine deiodinases","volume":"25","author":"Renko","year":"2015","journal-title":"Thyroid"},{"issue":"1\u20132","key":"10.1016\/j.tiv.2019.104662_bb0170","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.tox.2004.06.041","article-title":"Endocrine active compounds affect thyrotropin and thyroid hormone levels in serum as well as endpoints of thyroid hormone action in liver, heart and kidney","volume":"205","author":"Schmutzler","year":"2004","journal-title":"Toxicology"},{"issue":"Suppl. 1","key":"10.1016\/j.tiv.2019.104662_bb0175","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1289\/ehp.9369","article-title":"Endocrine disruptors and the thyroid gland\u2014a combined in vitro and in vivo analysis of potential new biomarkers","volume":"115","author":"Schmutzler","year":"2007","journal-title":"Environ. Health Perspect."},{"issue":"4","key":"10.1016\/j.tiv.2019.104662_bb0180","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1261\/rna.2192803","article-title":"Lentivirus-delivered stable gene silencing by RNAi in primary cells","volume":"9","author":"Stewart","year":"2003","journal-title":"RNA"},{"issue":"8","key":"10.1016\/j.tiv.2019.104662_bb0185","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1002\/jat.1790","article-title":"Developing in vitro reporter gene assays to assess the hormone receptor activities of chemicals frequently detected in drinking water","volume":"32","author":"Sun","year":"2012","journal-title":"J. Appl. Toxicol."},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0190","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1006\/abbi.1996.0222","article-title":"Mechanism of simultaneous iodination and coupling catalyzed by thyroid peroxidase","volume":"330","author":"Taurog","year":"1996","journal-title":"Arch. Biochem. Biophys."},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0195","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ab.2009.08.038","article-title":"A nonradioactive iodide uptake assay for sodium iodide symporter function","volume":"396","author":"Waltz","year":"2010","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.tiv.2019.104662_bb0200","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/10915810701876679","article-title":"Two-generation reproductive toxicity study of resorcinol administered via drinking water to Crl:CD(SD) rats","volume":"27","author":"Welsch","year":"2008","journal-title":"Int. J. Toxicol."},{"issue":"1\u20132","key":"10.1016\/j.tiv.2019.104662_bb0205","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1080\/10408440601123446","article-title":"General background on the hypothalamic-pituitary-thyroid (HPT) axis","volume":"37","author":"Zoeller","year":"2007","journal-title":"Crit. Rev. Toxicol."}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233319304138?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233319304138?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:34:26Z","timestamp":1760135666000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233319304138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":41,"alternative-id":["S0887233319304138"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2019.104662","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A rapid assay of human thyroid peroxidase activity","name":"articletitle","label":"Article Title"},{"value":"Toxicology in Vitro","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tiv.2019.104662","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2019 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"104662"}}