{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:34:11Z","timestamp":1790775251106,"version":"4.1.0"},"reference-count":124,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T00:00:00Z","timestamp":1604707200000},"content-version":"am","delay-in-days":280,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100007486","name":"Universit\u00e9 Paris-Sud","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007486","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.tiv.2019.104691","type":"journal-article","created":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T02:08:23Z","timestamp":1571710103000},"page":"104691","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"special_numbering":"C","title":["Immune-competent in vitro co-culture models as an approach for skin sensitisation assessment"],"prefix":"10.1016","volume":"62","author":[{"given":"Am\u00e9lie","family":"Th\u00e9lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Catoire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saadia","family":"Kerdine-R\u00f6mer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2019.104691_bb0005","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.tibtech.2015.04.003","article-title":"Strategies for improving the physiological relevance of human engineered tissues","volume":"33","author":"Abbott","year":"2015","journal-title":"Trends Biotechnol"},{"key":"10.1016\/j.tiv.2019.104691_bb0010","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3109\/1547691X.2012.711782","article-title":"Danger, intracellular signaling, and the orchestration of dendritic cell function in skin sensitization","volume":"10","author":"Ainscough","year":"2013","journal-title":"J Immunotoxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0015","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1097\/ACI.0b013e32833e08ae","article-title":"Keratinocytes in allergic skin diseases","volume":"10","author":"Albanesi","year":"2010","journal-title":"Curr Opin Allergy Clin Immunol"},{"key":"10.1016\/j.tiv.2019.104691_bb0020","doi-asserted-by":"crossref","first-page":"441","DOI":"10.14573\/altex1406111","article-title":"State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology","volume":"31","author":"Alepee","year":"2014","journal-title":"ALTEX"},{"key":"10.1016\/j.tiv.2019.104691_bb0025","series-title":"Prospective multicentre study of the U-SENS test method for skin sensitization testing: Toxicol In Vitro","author":"Alepee","year":"2015"},{"key":"10.1016\/j.tiv.2019.104691_bb0030","first-page":"424203","article-title":"The LLNA: A brief review of recent advances and limitations","author":"Anderson","year":"2011","journal-title":"J Allergy (Cairo),"},{"key":"10.1016\/j.tiv.2019.104691_bb0035","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.tiv.2005.10.012","article-title":"Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol","volume":"20","author":"Ashikaga","year":"2006","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0040","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s40204-018-0087-0","article-title":"Skin bioprinting: a novel approach for creating artificial skin from synthetic and natural building blocks","volume":"2","author":"Augustine","year":"2018","journal-title":"Prog Biomater,"},{"key":"10.1016\/j.tiv.2019.104691_bb0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tox.2009.07.021","article-title":"A plasmacytoid dendritic cell (CD123+\/CD11c-) based assay system to predict contact allergenicity of chemicals","volume":"264","author":"Ayehunie","year":"2009","journal-title":"Toxicology"},{"key":"10.1016\/j.tiv.2019.104691_bb0050","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.cbi.2014.09.002","article-title":"Development of a co-culture of keratinocytes and immune cells for in vitro investigation of cutaneous sulfur mustard toxicity","volume":"223C","author":"Balszuweit","year":"2014","journal-title":"Chem Biol Interact"},{"key":"10.1016\/j.tiv.2019.104691_bb0055","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1046\/j.1523-1747.2001.01247.x","article-title":"A strikingly constant ratio exists between Langerhans cells and other epidermal cells in human skin. A stereologic study using the optical disector method and the confocal laser scanning microscope","volume":"116","author":"Bauer","year":"2001","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0060","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1089\/ten.2006.0405","article-title":"Effects of solar ultraviolet radiation on engineered human skin equivalent containing both Langerhans cells and dermal dendritic cells","volume":"13","author":"Bechetoille","year":"2007","journal-title":"Tissue Eng"},{"key":"10.1016\/j.tiv.2019.104691_bb0065","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.addr.2014.01.013","article-title":"Consideration of the cellular microenvironment: physiologically relevant co-culture systems in drug discovery","volume":"69-70","author":"Berg","year":"2014","journal-title":"Adv Drug Deliv Rev"},{"key":"10.1016\/j.tiv.2019.104691_bb0070","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.tiv.2017.09.019","article-title":"Characterization of reconstructed human skin containing Langerhans cells to monitor molecular events in skin sensitization","volume":"46","author":"Bock","year":"2018","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0075","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/biot.201300054","article-title":"Studying cell-cell communication in co-culture","volume":"8","author":"Bogdanowicz","year":"2013","journal-title":"Biotechnol J"},{"key":"10.1016\/j.tiv.2019.104691_bb0080","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/7651_2013_62","article-title":"Multifunction co-culture model for evaluating cell-cell interactions","volume":"1202","author":"Bogdanowicz","year":"2014","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.tiv.2019.104691_bb0085","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nrmicro.2017.157","article-title":"The human skin microbiome","volume":"16","author":"Byrd","year":"2018","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.tiv.2019.104691_bb0090","doi-asserted-by":"crossref","first-page":"196","DOI":"10.3109\/08923973.2011.591800","article-title":"Evaluation of the skin sensitization potential of chemicals in THP-1\/keratinocyte co-cultures","volume":"34","author":"Cao","year":"2012","journal-title":"Immunopharmacol Immunotoxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0095","doi-asserted-by":"crossref","first-page":"035011","DOI":"10.1088\/1758-5082\/5\/3\/035011","article-title":"The development of a 3D immunocompetent model of human skin","volume":"5","author":"Chau","year":"2013","journal-title":"Biofabrication"},{"key":"10.1016\/j.tiv.2019.104691_bb0100","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nature25177","article-title":"Skin microbiota-host interactions","volume":"553","author":"Chen","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.tiv.2019.104691_bb0105","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.tox.2013.11.009","article-title":"Pyridoxylamine reactivity kinetics as an amine based nucleophile for screening electrophilic dermal sensitizers","volume":"315","author":"Chipinda","year":"2014","journal-title":"Toxicology"},{"key":"10.1016\/j.tiv.2019.104691_bb0110","first-page":"87","article-title":"Development of skin inflammation test model by co-culture of reconstituted 3D skin and RAW264.7 cells: Tissue Engineering and","volume":"11","author":"Chung","year":"2014","journal-title":"Regenerative Med"},{"key":"10.1016\/j.tiv.2019.104691_bb0115","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1177\/026119290503300313","article-title":"Guidance on good cell culture practice","volume":"33","author":"Coecke","year":"2005","journal-title":"Altern Lab Anim"},{"key":"10.1016\/j.tiv.2019.104691_bb0120","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.tiv.2015.02.012","article-title":"Genes specifically modulated in sensitized skins allow the detection of sensitizers in a reconstructed human skin model. Development of the SENS-IS assay","volume":"29","author":"Cottrez","year":"2015","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0125","first-page":"269","article-title":"Co-culture assay for the identification and investigation of dermal sensitizers (Epi-DC)","volume":"138","author":"Degeorge","year":"2014","journal-title":"Toxicologist"},{"key":"10.1016\/j.tiv.2019.104691_bb0130","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.taap.2009.02.004","article-title":"Progress on the development of human in vitro dendritic cell based assays for assessment of the sensitizing potential of a compound","volume":"236","author":"dos Santos","year":"2009","journal-title":"Toxicol Appl Pharmacol"},{"key":"10.1016\/j.tiv.2019.104691_bb0135","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1089\/aivt.2015.0003","article-title":"Review of the availability of in vitro and in silico methods for assessing dermal bioavailability","volume":"1","author":"Dumont","year":"2015","journal-title":"Applied In Vitro Toxicology"},{"key":"10.1016\/j.tiv.2019.104691_bb0140","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.taap.2010.03.009","article-title":"Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro","volume":"245","author":"Emter","year":"2010","journal-title":"Toxicol Appl Pharmacol"},{"key":"10.1016\/j.tiv.2019.104691_bb0145","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.tiv.2017.04.022","article-title":"Good cell culture practices & in vitro toxicology","volume":"45","author":"Eskes","year":"2017","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0150","series-title":"The Evolution of Three-Dimensional Cell Cultures Towards Unimpeded Regenerative Medicine and Tissue Engineering","author":"Evangelatov","year":"2013"},{"issue":"12","key":"10.1016\/j.tiv.2019.104691_bb0155","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1007\/s00204-016-1842-4","article-title":"State of the art in non-animal approaches for skin sensitization testing: from individual test methods towards testing strategies","volume":"90","author":"Ezendam","year":"2016","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0160","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1046\/j.0022-202X.2004.22209.x","article-title":"Langerhans cells integrated into human reconstructed epidermis respond to known sensitizers and ultraviolet exposure","volume":"122","author":"Facy","year":"2004","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0165","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.tiv.2005.03.018","article-title":"Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation","volume":"19","author":"Facy","year":"2005","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0170","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1046\/j.1365-2133.1998.02454.x","article-title":"Culture of human epidermal Langerhans cells in a skin equivalent","volume":"139","author":"Fransson","year":"1998","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0175","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1007\/s00204-015-1527-4","article-title":"Unsaturated compounds induce up-regulation of CD86 on dendritic cells in the in vitro sensitization assay LCSA","volume":"90","author":"Frohwein","year":"2016","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0180","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s00204-016-1722-y","article-title":"A novel method to generate monocyte-derived dendritic cells during coculture with HaCaT facilitates detection of weak contact allergens in cosmetics","volume":"91","author":"Frombach","year":"2017","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0185","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1007\/s00204-018-2164-5","article-title":"Lymphocyte surface markers and cytokines are suitable for detection and potency assessment of skin-sensitizing chemicals in an in vitro model of allergic contact dermatitis: the LCSA-ly","volume":"92","author":"Frombach","year":"2018","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0190","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.vascn.2014.06.001","article-title":"Development of a prediction method for skin sensitization using novel cysteine and lysine derivatives","volume":"70","author":"Fujita","year":"2014","journal-title":"J Pharmacol Toxicol Methods"},{"key":"10.1016\/j.tiv.2019.104691_bb0195","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1093\/toxsci\/kfh213","article-title":"Development of a peptide reactivity assay for screening contact allergens","volume":"81","author":"Gerberick","year":"2004","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0200","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1093\/toxsci\/kfp192","article-title":"Investigation of peptide reactivity of pro-hapten skin sensitizers using a peroxidase-peroxide oxidation system","volume":"112","author":"Gerberick","year":"2009","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0205","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.taap.2013.07.003","article-title":"An epidermal equivalent assay for identification and ranking potency of contact sensitizers","volume":"272","author":"Gibbs","year":"2013","journal-title":"Toxicol Appl Pharmacol"},{"key":"10.1016\/j.tiv.2019.104691_bb0210","doi-asserted-by":"crossref","first-page":"e0136713","DOI":"10.1371\/journal.pone.0136713","article-title":"Melanin transfer in human 3D skin equivalents generated exclusively from induced pluripotent stem cells","volume":"10","author":"Gledhill","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.tiv.2019.104691_bb0215","doi-asserted-by":"crossref","DOI":"10.1098\/rsif.2014.0065","article-title":"Co-culture systems and technologies: taking synthetic biology to the next level","volume":"11","author":"Goers","year":"2014","journal-title":"J R Soc Interface"},{"key":"10.1016\/j.tiv.2019.104691_bb0220","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/S0140-6736(09)61496-3","article-title":"Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study","volume":"374","author":"Guenou","year":"2009","journal-title":"Lancet"},{"key":"10.1016\/j.tiv.2019.104691_bb0225","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1046\/j.1523-1747.2001.01355.x","article-title":"Phenotypic and functional outcome of human monocytes or monocyte-derived dendritic cells in a dermal equivalent","volume":"116","author":"Guironnet","year":"2001","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0230","doi-asserted-by":"crossref","first-page":"vi","DOI":"10.1016\/j.addr.2014.04.003","article-title":"3D - a new dimension of in vitro research","volume":"69-70","author":"Hartung","year":"2014","journal-title":"Adv Drug Deliv Rev"},{"issue":"2","key":"10.1016\/j.tiv.2019.104691_bb0235","first-page":"279","article-title":"Keratinocytes improve prediction of sensitization potential and potency of chemicals with THP-1 cells","volume":"34","author":"Hennen","year":"2016","journal-title":"ALTEX"},{"key":"10.1016\/j.tiv.2019.104691_bb0240","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1093\/toxsci\/kfr174","article-title":"Cross talk between keratinocytes and dendritic cells: impact on the prediction of sensitization","volume":"123","author":"Hennen","year":"2011","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0245","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1574-6968.2008.01402.x","article-title":"Differential innate immune responses of a living skin equivalent model colonized by Staphylococcus epidermidis or Staphylococcus aureus","volume":"290","author":"Holland","year":"2009","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.tiv.2019.104691_bb0250","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1089\/aivt.2016.0003","article-title":"Organs-on-chips as bridges for predictive toxicology","volume":"2","author":"Hutson","year":"2016","journal-title":"Appl In Vitro Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0255","doi-asserted-by":"crossref","first-page":"e77673","DOI":"10.1371\/journal.pone.0077673","article-title":"Generation of 3D skin equivalents fully reconstituted from human induced pluripotent stem cells (iPSCs)","volume":"8","author":"Itoh","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.tiv.2019.104691_bb0260","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/j.tiv.2012.05.019","article-title":"The GARD assay for assessment of chemical skin sensitizers","volume":"27","author":"Johansson","year":"2013","journal-title":"Toxicol In Vitro"},{"issue":"7","key":"10.1016\/j.tiv.2019.104691_bb0265","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1016\/j.tiv.2015.07.006","article-title":"Optimization of the IL-8 Luc assay as an in vitro test for skin sensitization","volume":"29","author":"Kimura","year":"2015","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0270","first-page":"1","article-title":"Non-animal methods to predict skin sensitization (II): an assessment of defined approaches","author":"Kleinstreuer","year":"2018","journal-title":"Crit Rev Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0275","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/cod.12789","article-title":"Current knowledge on biomarkers for contact sensitization and allergic contact dermatitis","volume":"77","author":"Koppes","year":"2017","journal-title":"Contact Dermatitis"},{"key":"10.1016\/j.tiv.2019.104691_bb0280","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.taap.2015.05.017","article-title":"MUTZ-3 derived Langerhans cells in human skin equivalents show differential migration and phenotypic plasticity after allergen or irritant exposure","volume":"287","author":"Kosten","year":"2015","journal-title":"Toxicol Appl Pharmacol"},{"key":"10.1016\/j.tiv.2019.104691_bb0285","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1084\/jem.172.5.1483","article-title":"Migration and maturation of Langerhans cells in skin transplants and explants","volume":"172","author":"Larsen","year":"1990","journal-title":"J Exp Med"},{"key":"10.1016\/j.tiv.2019.104691_bb0290","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s00403-010-1092-x","article-title":"Integration of Langerhans-like cells into a human skin equivalent","volume":"303","author":"Laubach","year":"2011","journal-title":"Arch Dermatol Res"},{"key":"10.1016\/j.tiv.2019.104691_bb0295","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/j.tiv.2014.07.002","article-title":"Predicting full thickness skin sensitization using a support vector machine","volume":"28","author":"Lee","year":"2014","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0300","first-page":"67","article-title":"Tri-culture system for pro-hapten sensitizer identification and potency classification","volume":"6","author":"Lee","year":"2018","journal-title":"Technology (Singap World Sci)"},{"key":"10.1016\/j.tiv.2019.104691_bb0305","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.tiv.2017.03.012","article-title":"Architecture in 3D cell culture: an essential feature for in vitro toxicology","volume":"45","author":"Lelievre","year":"2017","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0310","doi-asserted-by":"crossref","DOI":"10.5430\/jhm.v1n1p35","article-title":"Stem cell-based dendritic cell vaccine development","volume":"1","author":"Li","year":"2011","journal-title":"J Hematol Malignancies"},{"key":"10.1016\/j.tiv.2019.104691_bb0315","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4252\/wjsc.v6.i1.1","article-title":"Dendritic cells derived from pluripotent stem cells: Potential of large scale production","volume":"6","author":"Li","year":"2014","journal-title":"World J Stem Cells"},{"key":"10.1016\/j.tiv.2019.104691_bb0320","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1111\/1523-1747.ep12349010","article-title":"Human cutaneous dendritic cells migrate through dermal lymphatic vessels in a skin organ culture model","volume":"106","author":"Lukas","year":"1996","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0325","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.addr.2013.12.006","article-title":"The use of skin models in drug development","volume":"69\u201370","author":"Mathes","year":"2014","journal-title":"Adv Drug Deliv Rev"},{"key":"10.1016\/j.tiv.2019.104691_bb0330","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2011.08.018","article-title":"Chemical toxicity to keratinocytes triggers dendritic cell activation via an IL-1alpha path","volume":"129","author":"Matjeka","year":"2012","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.tiv.2019.104691_bb0335","first-page":"90","article-title":"New approach for chemical sensitizing potential assessment using THP-1 and NCTC 2544 co-culture","volume":"27","author":"Meloni","year":"2010","journal-title":"ALTEX"},{"key":"10.1016\/j.tiv.2019.104691_bb0340","doi-asserted-by":"crossref","first-page":"929","DOI":"10.3389\/fimmu.2017.00929","article-title":"Prediction of chemical respiratory and contact sensitizers by OX40L expression in dendritic cells using a novel 3D coculture system","volume":"8","author":"Mizoguchi","year":"2017","journal-title":"Front Immunol"},{"issue":"10","key":"10.1016\/j.tiv.2019.104691_bb0345","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1007\/s00204-015-1555-0","article-title":"Reporter cell lines for skin sensitization testing","volume":"89","author":"Natsch","year":"2015","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0350","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1093\/toxsci\/kft160","article-title":"Utility of rat liver S9 fractions to study skin-sensitizing prohaptens in a modified KeratinoSens assay","volume":"135","author":"Natsch","year":"2013","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0355","doi-asserted-by":"crossref","first-page":"3453","DOI":"10.1007\/s00018-016-2252-8","article-title":"Methodologies in creating skin substitutes","volume":"73","author":"Nicholas","year":"2016","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0360","series-title":"The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins Part 1 Scientific Evidence","first-page":"59","volume":"168","author":"OECD","year":"2012"},{"key":"10.1016\/j.tiv.2019.104691_bb0365","series-title":"The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins Part 2 Use of the AOP to Develop Chemical Categories and Integrated Assessment and Testing Approaches","first-page":"46","volume":"168","author":"OECD","year":"2012"},{"key":"10.1016\/j.tiv.2019.104691_bb0370","series-title":"OECD GD 255 Guidance document of the reporting of defined approaches to be used within integrated approaches to testing and assessment","year":"2016"},{"key":"10.1016\/j.tiv.2019.104691_bb0375","series-title":"OECD GD 256 Guidance document on the reporting of defined approaches and individual information sources to be used within integrated approaches to testing and assessment (IATA) for skin sensitisation","year":"2016"},{"key":"10.1016\/j.tiv.2019.104691_bb0380","series-title":"OECD TG 442D In vitro skin sensitisation assays addressing the AOP key event on keratinocyte activation","year":"2018"},{"key":"10.1016\/j.tiv.2019.104691_bb0385","series-title":"OECD TG 442E in vitro skin sensitisation assays adressing the key event on activation of dendritic cells on the adverse outcome pathway for skin sensitisation","year":"2018"},{"key":"10.1016\/j.tiv.2019.104691_bb0390","series-title":"OECD TG 442C In Chemico Skin Sensitisation Assays addressing the Adverse Outcome Pathway key event on covalent binding to proteins","year":"2019"},{"key":"10.1016\/j.tiv.2019.104691_bb0395","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/j.1365-2567.2008.02919.x","article-title":"TGF-beta1 dampens the susceptibility of dendritic cells to environmental stimulation, leading to the requirement for danger signals for activation","volume":"126","author":"Ohtani","year":"2009","journal-title":"Immunology"},{"key":"10.1016\/j.tiv.2019.104691_bb0400","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1002\/eji.200838384","article-title":"CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis","volume":"38","author":"Ouwehand","year":"2008","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.tiv.2019.104691_bb0405","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1002\/eji.200940150","article-title":"Epidermis-to-dermis migration of immature Langerhans cells upon topical irritant exposure is dependent on CCL2 and CCL5","volume":"40","author":"Ouwehand","year":"2010","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.tiv.2019.104691_bb0410","series-title":"Progress Towards Novel Testing Strategies for in Vitro Assessment of Allergens","first-page":"55","article-title":"The impact of sensitizing compounds on the interactions between epithelial cells and dendritic cells","author":"Ouwehand","year":"2011"},{"key":"10.1016\/j.tiv.2019.104691_bb0415","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1189\/jlb.0610374","article-title":"Technical advance: Langerhans cells derived from a human cell line in a full-thickness skin equivalent undergo allergen-induced maturation and migration","volume":"90","author":"Ouwehand","year":"2011","journal-title":"J Leukoc Biol"},{"key":"10.1016\/j.tiv.2019.104691_bb0420","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.ejcb.2012.06.004","article-title":"CCL5 and CCL20 mediate immigration of Langerhans cells into the epidermis of full thickness human skin equivalents","volume":"91","author":"Ouwehand","year":"2012","journal-title":"Eur J Cell Biol"},{"key":"10.1016\/j.tiv.2019.104691_bb0425","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00018-011-0846-8","article-title":"Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects. Current knowledge assembled at an international workshop at BfR, Germany","volume":"69","author":"Peiser","year":"2012","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0430","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.stemcr.2014.03.009","article-title":"3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells","volume":"2","author":"Petrova","year":"2014","journal-title":"Stem Cell Reports"},{"key":"10.1016\/j.tiv.2019.104691_bb0435","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.tiv.2015.03.009","article-title":"The Myeloid U937 Skin Sensitization Test (U-SENS) addresses the activation of dendritic cell event in the adverse outcome pathway for skin sensitization","volume":"29","author":"Piroird","year":"2015","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0440","series-title":"Lack of desquamation - the Achilles heel of the reconstructed epidermis","author":"Ponec","year":"2002"},{"key":"10.1016\/j.tiv.2019.104691_bb0445","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s00204-018-2152-9","article-title":"Innovative organotypic in vitro models for safety assessment: aligning with regulatory requirements and understanding models of the heart, skin, and liver as paradigms","volume":"92","author":"Pridgeon","year":"2018","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0450","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1111\/j.1365-2133.1996.tb06961.x","article-title":"Integrity of the permeability barrier regulates epidermal Langerhans cell density","volume":"134","author":"Proksch","year":"1996","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0455","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1039\/C6LC00229C","article-title":"In vitro micro-physiological immune-competent model of the human skin","volume":"16","author":"Ramadan","year":"2016","journal-title":"Lab Chip"},{"key":"10.1016\/j.tiv.2019.104691_bb0460","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1016\/j.tiv.2014.08.002","article-title":"LuSens: a keratinocyte based ARE reporter gene assay for use in integrated testing strategies for skin sensitization hazard identification","volume":"28","author":"Ramirez","year":"2014","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0465","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/1523-1747.ep12336627","article-title":"Integration of Langerhans Cells into a Pigmented Reconstructed Human Epidermis","volume":"109","author":"R\u00e9gnier","year":"1997","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0470","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1016\/j.tiv.2012.08.007","article-title":"Human T cell priming assay (hTCPA) for the identification of contact allergens based on naive T cells and DC--IFN-gamma and TNF-alpha readout","volume":"27","author":"Richter","year":"2013","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0475","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.addr.2013.12.005","article-title":"The application of 3D cell models to support drug safety assessment: opportunities & challenges","volume":"69-70","author":"Roth","year":"2014","journal-title":"Adv Drug Deliv Rev"},{"key":"10.1016\/j.tiv.2019.104691_bb0480","doi-asserted-by":"crossref","first-page":"4966","DOI":"10.4049\/jimmunol.178.8.4966","article-title":"Dermal fibroblasts induce maturation of dendritic cells","volume":"178","author":"Saalbach","year":"2007","journal-title":"J Immunol"},{"key":"10.1016\/j.tiv.2019.104691_bb0485","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1038\/jid.2009.253","article-title":"Dermal fibroblasts promote the migration of dendritic cells","volume":"130","author":"Saalbach","year":"2010","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0490","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1111\/exd.12722","article-title":"Fibroblasts support migration of monocyte-derived dendritic cells by secretion of PGE2 and MMP-1","volume":"24","author":"Saalbach","year":"2015","journal-title":"Exp Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0495","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1016\/j.tiv.2013.08.007","article-title":"Development of a new in vitro skin sensitization assay (Epidermal Sensitization Assay; EpiSensA) using reconstructed human epidermis","volume":"27","author":"Saito","year":"2013","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0500","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.tiv.2005.10.014","article-title":"Development of an in vitro skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT","volume":"20","author":"Sakaguchi","year":"2006","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0505","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.immuni.2005.08.012","article-title":"Cutaneous CXCL14 targets blood precursors to epidermal niches for Langerhans cell differentiation","volume":"23","author":"Schaerli","year":"2005","journal-title":"Immunity"},{"key":"10.1016\/j.tiv.2019.104691_bb0510","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1177\/026119290803600207","article-title":"The use of reconstructed human epidermis for skin absorption testing: Results of the validation study","volume":"36","author":"Schafer-Korting","year":"2008","journal-title":"Altern Lab Anim"},{"key":"10.1016\/j.tiv.2019.104691_bb0515","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.tiv.2019.02.002","article-title":"A coculture system composed of THP-1 cells and 3D reconstructed human epidermis to assess activation of dendritic cells by sensitizing chemicals after topical exposure","volume":"57","author":"Schellenberger","year":"2019","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0520","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1111\/j.1398-9995.2007.01511.x","article-title":"A loose-fit coculture of activated keratinocytes and dendritic cell-related cells for prediction of sensitizing potential","volume":"62","author":"Schreiner","year":"2007","journal-title":"Allergy"},{"key":"10.1016\/j.tiv.2019.104691_bb0525","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tox.2008.04.020","article-title":"A new dendritic cell type suitable as sentinel of contact allergens","volume":"249","author":"Schreiner","year":"2008","journal-title":"Toxicology"},{"key":"10.1016\/j.tiv.2019.104691_bb0530","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s00204-012-0811-9","article-title":"Assessment of the sensitizing potential of textile disperse dyes and some of their metabolites by the loose-fit coculture-based sensitization assay (LCSA)","volume":"86","author":"Sonnenburg","year":"2012","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0535","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1007\/s00204-014-1406-4","article-title":"Assessment of the sensitizing potency of preservatives with chance of skin contact by the loose-fit coculture-based sensitization assay (LCSA)","volume":"89","author":"Sonnenburg","year":"2015","journal-title":"Arch Toxicol"},{"key":"10.1016\/j.tiv.2019.104691_bb0540","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.mattod.2017.11.002","article-title":"Full-thickness human skin-on-chip with enhanced epidermal morphogenesis and barrier function","volume":"21","author":"Sriram","year":"2018","journal-title":"Materials Today"},{"key":"10.1016\/j.tiv.2019.104691_bb0545","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1089\/ten.tec.2014.0065","article-title":"Lowered humidity produces human epidermal equivalents with enhanced barrier properties","volume":"21","author":"Sun","year":"2015","journal-title":"Tissue Eng Part C Methods"},{"key":"10.1016\/j.tiv.2019.104691_bb0550","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1093\/toxsci\/kfr237","article-title":"An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8","volume":"124","author":"Takahashi","year":"2011","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.tiv.2019.104691_bb0555","first-page":"341","article-title":"Skin sensitisation potential: addressing the 3 key events of AOP using a 3D keratinocytes \/ THP-1 co-culture","volume":"168","author":"Th\u00e9lu","year":"2019","journal-title":"Toxicologist"},{"key":"10.1016\/j.tiv.2019.104691_bb0560","first-page":"341","article-title":"A new method for skin sensitisation potential for poorly soluble compounds using a 3D keratinocyte \/ THP-1 co-culture","volume":"168","author":"Th\u00e9lu","year":"2019","journal-title":"Toxicologist"},{"key":"10.1016\/j.tiv.2019.104691_bb0565","first-page":"8","article-title":"Progress on the society of toxicology Colgate Palmolive 2014 Grant for alternative research: in vitro assay for identification of dermal sensitizers","volume":"144","author":"Troese","year":"2015","journal-title":"Toxicologist"},{"key":"10.1016\/j.tiv.2019.104691_bb0570","first-page":"4407","article-title":"Update on the Society of Toxicology \u2013 Colgate Palmolive Grant for Alternative Research: in vitro coculture assay for identification of dermal sensitizers","volume":"150","author":"Troese","year":"2016","journal-title":"Toxicologist"},{"key":"10.1016\/j.tiv.2019.104691_bb0575","doi-asserted-by":"crossref","first-page":"246","DOI":"10.5107\/sccj.41.4_246","article-title":"Construction of a three-dimensional human skin model consisting of keratinocytes, dendritic cells and fibroblasts and application of this model for alternative animal testing of immune-sensitizing compounds","volume":"41","author":"Uchino","year":"2007","journal-title":"J. Soc. Cosmet. Chem. Jap."},{"key":"10.1016\/j.tiv.2019.104691_bb0580","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1248\/yakushi.128.45","article-title":"Construction of three-dimensional human skin model involving dendritic cells and its application to skin sensitization test","volume":"128","author":"Uchino","year":"2008","journal-title":"Yakugaku Zasshi"},{"key":"10.1016\/j.tiv.2019.104691_bb0585","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.tiv.2008.12.003","article-title":"Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane","volume":"23","author":"Uchino","year":"2009","journal-title":"Toxicol In Vitro"},{"key":"10.1016\/j.tiv.2019.104691_bb0590","first-page":"1","article-title":"Development of an alternative test for skin sensitization using a three-dimensional human skin model consisting of dendritic cells, keratinocytes and fibroblasts","volume":"16","author":"Uchino","year":"2011","journal-title":"AATEX"},{"key":"10.1016\/j.tiv.2019.104691_bb0595","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/jid.2013.417","article-title":"Crosstalk between keratinocytes and T cells in a 3D microenvironment: a model to study inflammatory skin diseases","volume":"134","author":"van den Bogaard","year":"2014","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.tiv.2019.104691_bb0600","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1007\/s12015-017-9737-1","article-title":"Progress and future prospectives in skin-on-chip development with emphasis on the use of different cell types and technical challenges","volume":"13","author":"van den Broek","year":"2017","journal-title":"Stem Cell Rev"},{"key":"10.1016\/j.tiv.2019.104691_bb0605","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1111\/j.1398-9995.2009.02082.x","article-title":"Effector and regulatory mechanisms in allergic contact dermatitis","volume":"64","author":"Vocanson","year":"2009","journal-title":"Allergy"},{"key":"10.1016\/j.tiv.2019.104691_bb0610","first-page":"115","article-title":"An in vitro assay to screen for the sensitizing potential of xenobiotics","volume":"25","author":"Wanner","year":"2008","journal-title":"ALTEX"},{"key":"10.1016\/j.tiv.2019.104691_bb0615","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.taap.2010.02.019","article-title":"Classification of sensitizing and irritative potential in a combined in-vitro assay","volume":"245","author":"Wanner","year":"2010","journal-title":"Toxicol Appl Pharmacol"},{"key":"10.1016\/j.tiv.2019.104691_bb0620","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.tiv.2017.08.015","article-title":"Prediction of skin sensitization potency sub-categories using peptide reactivity data","volume":"45","author":"Wareing","year":"2017","journal-title":"Toxicol In Vitro"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233319305375?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233319305375?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:36:05Z","timestamp":1760135765000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233319305375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":124,"alternative-id":["S0887233319305375"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2019.104691","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immune-competent in vitro co-culture models as an approach for skin sensitisation assessment","name":"articletitle","label":"Article Title"},{"value":"Toxicology in Vitro","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tiv.2019.104691","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104691"}}