{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T12:51:41Z","timestamp":1789735901707,"version":"4.0.1"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100006162","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia do Estado de Pernambuco","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006162","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology in Vitro"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.tiv.2020.104771","type":"journal-article","created":{"date-parts":[[2020,1,11]],"date-time":"2020-01-11T11:10:01Z","timestamp":1578741001000},"page":"104771","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Barbatic acid from Cladia aggregata (lichen): Cytotoxicity and in vitro schistosomicidal evaluation and ultrastructural analysis against adult worms of Schistosoma mansoni"],"prefix":"10.1016","volume":"65","author":[{"given":"H.A.M.F.","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.L.","family":"Aires","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.L.R.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.L.F.","family":"S\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.C.B.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.C.P.A.","family":"Albuquerque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.G.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.A.","family":"Brayner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.C.","family":"Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.M.M.A.","family":"Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.H.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tiv.2020.104771_bb0005","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.exppara.2014.04.010","article-title":"Ultrastructural analysis of \u03b2-lapachone-induced surface membrane damage in male adult Schistosoma mansoni BH strain worms","volume":"142","author":"Aires","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0010","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.actatropica.2014.06.005","article-title":"Toxic effects of Microgramma vacciniifolia rhizome lectin on Artemia salina, human cells, and the schistosomiasis vector Biomphalaria glabrata","volume":"138","author":"Albuquerque","year":"2014","journal-title":"Acta Trop."},{"key":"10.1016\/j.tiv.2020.104771_bb0015","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1017\/S0022149X13000278","article-title":"In vitro and in vivo effects of hesperidin treatment on adult worms of Schistosoma mansoni","volume":"88","author":"Allam","year":"2014","journal-title":"J. Helminthol."},{"key":"10.1016\/j.tiv.2020.104771_bb0020","doi-asserted-by":"crossref","first-page":"8821","DOI":"10.1021\/acs.jafc.6b04200","article-title":"Schistosomicidal activity of alkyl-phenols from the cashew Anacardium occidentale against Schistosoma mansoni adult worms","volume":"64","author":"Alvarenga","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.tiv.2020.104771_bb0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2018.12.024","article-title":"Usnic acid potassium salt from Cladonia substellata (lichen): synthesis, cytotoxicity and in vitro anthelmintic activity and ultrastructural analysis against adult worms of Schistosoma mansoni","volume":"192","author":"Ara\u00fajo","year":"2019","journal-title":"Acta Trop."},{"key":"10.1016\/j.tiv.2020.104771_bb0030","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1007\/s00436-011-2786-5","article-title":"Ultrastructural analysis of miltefosine-induced surface membrane damage in adult Schistosoma mansoni BH strain worms","volume":"110","author":"Bert\u00e3o","year":"2012","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.tiv.2020.104771_bb0035","first-page":"256","article-title":"Effects of fumarprotocetraric acid, a depsidone from the lichen Cladonia verticillaris, on tyrosinase activity","volume":"9","author":"Brand\u00e3o","year":"2017","journal-title":"Orbital: Electron. J. Chem."},{"key":"10.1016\/j.tiv.2020.104771_bb0040","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.tiv.2017.07.021","article-title":"Cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects","volume":"44","author":"Brito","year":"2017","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.tiv.2020.104771_bb0045","series-title":"Neuroepidemiology in Tropical Health","first-page":"233","article-title":"Neurological syndromes or diseases caused by parasites in tropical areas","author":"Carod-Artal","year":"2017"},{"key":"10.1016\/j.tiv.2020.104771_bb0050","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1016\/S0140-6736(13)61949-2","article-title":"Human schistosomiasis","volume":"383","author":"Colley","year":"2014","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.tiv.2020.104771_bb0055","doi-asserted-by":"crossref","first-page":"20954","DOI":"10.1038\/srep20954","article-title":"Whole genome resequencing of the human parasite Schistosoma mansoni reveals population history and effects of selection","volume":"16","author":"Crellen","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.tiv.2020.104771_bb0060","series-title":"Chemical and Botanical Guide to Lichen Products","author":"Culberson","year":"1969"},{"key":"10.1016\/j.tiv.2020.104771_bb0065","doi-asserted-by":"crossref","first-page":"701","DOI":"10.4155\/fmc.15.11","article-title":"What is the mechanism of action of praziquantel and how might resistance strike?","volume":"7","author":"Cupit","year":"2015","journal-title":"Future Med. Chem."},{"key":"10.1016\/j.tiv.2020.104771_bb0070","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1007\/s00436-015-4341-2","volume":"114","author":"El-Shabasy","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.tiv.2020.104771_bb0075","doi-asserted-by":"crossref","first-page":"477","DOI":"10.3347\/kjp.2016.54.4.477","article-title":"In vitro schistosomicidal activity of phytol and tegumental alterations induced in juvenile and adult stages of Schistosoma haematobium","volume":"54","author":"Eraky","year":"2016","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0080","doi-asserted-by":"crossref","first-page":"397","DOI":"10.2307\/3281679","article-title":"Schistosoma mansoni: production of cercarial eicosanoids as correlates of penetration and transformation","volume":"72","author":"Fusco","year":"1986","journal-title":"J. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0085","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0014-4894(87)90137-8","article-title":"Schistosoma mansoni: pH dependence of cercarial eicosanoid production, penetration, and transformation","volume":"64","author":"Fusco","year":"1987","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0090","doi-asserted-by":"crossref","first-page":"10873","DOI":"10.3390\/molecules200610873","article-title":"Anti-schistosomal activity of cinnamic acid esters: eugenyl and thymyl cinnamate induce cytoplasmic vacuoles and death in schistosomula of Schistosoma mansoni","volume":"20","author":"Glaser","year":"2015","journal-title":"Molecules"},{"key":"10.1016\/j.tiv.2020.104771_bb0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3823\/2594","article-title":"Antineoplastic activity and genotoxicity of organic extracts and barbatic acid isolated from the lichen Cladonia Salzmannii Nyl","volume":"11","author":"Gon\u00e7alves","year":"2018","journal-title":"Int. Arch. Med."},{"key":"10.1016\/j.tiv.2020.104771_bb0100","doi-asserted-by":"crossref","DOI":"10.3390\/ph11010015","article-title":"Drug repurposing for schistosomiasis: combinations of drugs or biomolecules","volume":"11","author":"Gouveia","year":"2018","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.tiv.2020.104771_bb0105","doi-asserted-by":"crossref","first-page":"S97","DOI":"10.1017\/S0031182005008346","article-title":"Ca2+ signalling, voltage-gated Ca2+ channels and praziquantel in flatworm neuromusculature","volume":"131","author":"Greenberg","year":"2005","journal-title":"Parasitology"},{"key":"10.1016\/j.tiv.2020.104771_bb0110","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0140-6736(06)69440-3","article-title":"Human Schistosomiasis","volume":"368","author":"Gryseels","year":"2006","journal-title":"Lancet"},{"key":"10.1016\/j.tiv.2020.104771_bb0115","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003656","article-title":"Anthelmintic activity in vivo of epiisopiloturine against juvenile and adult worms of Schistosoma mansoni","volume":"9","author":"Guimar\u00e3es","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.tiv.2020.104771_bb0120","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.pt.2018.10.005","article-title":"Advancing the development of a human schistosomiasis vaccine","volume":"35","author":"Hotez","year":"2019","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0125","series-title":"Identification of Lichen Substances","author":"Huneck","year":"1996"},{"key":"10.1016\/j.tiv.2020.104771_bb0130","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/S0223-5234(99)00132-4","article-title":"Depsides as non-redox inhibitors of leukotriene B4 biosynthesis and HaCaT cell growth. 1. Novel analogues of barbatic and diffractaic acid","volume":"34","author":"Kumar","year":"1999","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.tiv.2020.104771_bb0135","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.exppara.2012.10.003","article-title":"The anthelmintic effect of plumbagin on Schistosoma mansoni","volume":"133","author":"Lorsuwannarat","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0140","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tiv.2018.02.012","article-title":"Antiparasitic, structural, pharmacokinetic, and toxicological properties of riparin derivatives","volume":"50","author":"Mafud","year":"2018","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.tiv.2020.104771_bb0145","first-page":"259","article-title":"Antinociceptive activity of crude extracts and Atranorin obtained from the lichen Cladina dendroides (des Abb.) Ahti","volume":"21","author":"Maia","year":"2002","journal-title":"Acta Farm. Bonaer."},{"key":"10.1016\/j.tiv.2020.104771_bb0150","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0111102","article-title":"Usnic acid potassiun salt: Na alternative for the control of Biomphalaria glabrata (say, 1818)","volume":"9","author":"Martins","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.tiv.2020.104771_bb0155","first-page":"1","article-title":"In vitro and in vivo antineoplastic activity of barbatic acid","volume":"9","author":"Martins","year":"2016","journal-title":"Int. Arch. Med."},{"key":"10.1016\/j.tiv.2020.104771_bb0160","doi-asserted-by":"crossref","first-page":"568","DOI":"10.3390\/molecules22040568","article-title":"Barbatic acid offers a new possibility for control of Biomphalaria glabrata and Schistosomiasis","volume":"22","author":"Martins","year":"2017","journal-title":"Molecules"},{"key":"10.1016\/j.tiv.2020.104771_bb0165","doi-asserted-by":"crossref","first-page":"79","DOI":"10.13102\/sociobiology.v65i1.1840","article-title":"Effects of usnic, barbatic and fumarprotocetraric acids on survival of nasutitermes corniger (isoptera: termitidae: nasutitermitinae)","volume":"65","author":"Martins","year":"2018","journal-title":"Sociobiology"},{"key":"10.1016\/j.tiv.2020.104771_bb0170","first-page":"1","article-title":"Lichens as source of versatile bioactive compounds","volume":"2","author":"Mitrovi\u0107","year":"2011","journal-title":"Biol. Nyssana"},{"key":"10.1016\/j.tiv.2020.104771_bb0175","doi-asserted-by":"crossref","first-page":"801","DOI":"10.4155\/fmc.15.23","article-title":"Natural products with antischistosomal activity","volume":"7","author":"Moraes","year":"2015","journal-title":"Future Med. Chem."},{"key":"10.1016\/j.tiv.2020.104771_bb0180","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.exppara.2014.02.010","article-title":"Anthelmintic activity in vitro and in vivo of Baccharis trimera (less) DC against immature and adult worms of Schistosoma mansoni","volume":"139","author":"Oliveira","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0185","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2307\/3274166","article-title":"An efficient method for exposure of mice to Cercariae of Schistosoma mansoni","volume":"38","author":"Olivier","year":"1952","journal-title":"J. Parasitol."},{"key":"10.1016\/j.tiv.2020.104771_bb0190","first-page":"205","article-title":"Determination of Teloschistes flavicans (sw) norm anti-inflammatory activity","volume":"2","author":"Pereira","year":"2010","journal-title":"Pharm. Res."},{"key":"10.1016\/j.tiv.2020.104771_bb0195","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.fitote.2016.09.003","article-title":"Herbal extracts, lichens and biomolecules as natural photo-protection alternatives to synthetic UV filters. A systematic review","volume":"114","author":"Radice","year":"2016","journal-title":"Fitoterapia"},{"key":"10.1016\/j.tiv.2020.104771_bb0200","series-title":"Lichen Secondary Metabolites","first-page":"1","article-title":"Lichens as a potential source of bioactive secondary metabolites","author":"Rankovi\u0107","year":"2015"},{"key":"10.1016\/j.tiv.2020.104771_bb0205","doi-asserted-by":"crossref","first-page":"PI304","DOI":"10.1055\/s-0032-1320991","article-title":"In vitro schistosomicidal activity of Usnea steineri extract and its major constituent (+)-usnic acid against Schistosoma mansoni","volume":"78","author":"Salloum","year":"2012","journal-title":"Planta Med."},{"key":"10.1016\/j.tiv.2020.104771_bb0210","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.vaccine.2015.10.027","article-title":"Schistosomiasis vaccine candidate Sm14\/GLA-SE: phase 1 safety and immunogenicity clinical trial in healthy, male adults","volume":"34","author":"Santini-Oliveira","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.tiv.2020.104771_bb0215","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/j.cell.2015.11.031","article-title":"A new golden age of natural products drug discovery","volume":"163","author":"Shen","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.tiv.2020.104771_bb0220","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.tiv.2014.12.014","article-title":"Garcinielliptone FC: antiparasitic activity without cytotoxicity to mammalian cells","volume":"29","author":"Silva","year":"2015","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tiv.2020.104771_bb0225","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cbi.2018.01.016","article-title":"New 1,3-benzodioxole derivatives: synthesis, evaluation of in vitro schistosomicidal activity and ultrastructural analysis","volume":"283","author":"Silva","year":"2018","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.tiv.2020.104771_bb0230","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1017\/S0031182000086248","article-title":"The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms","volume":"55","author":"Smithers","year":"1965","journal-title":"Parasitology"},{"key":"10.1016\/j.tiv.2020.104771_bb0235","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1080\/14786419.2010.529546","article-title":"Antioxidant activity of some lichen metabolites","volume":"25","author":"Thadhani","year":"2011","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.tiv.2020.104771_bb0240","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.02582-16","article-title":"Praziquantel for schistosomiasis, single drug revisited metabolism mode of action and resistence","volume":"61","author":"Vale","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.tiv.2020.104771_bb0245","series-title":"Recent Advances in Lichenology","first-page":"147","article-title":"In Vitro culture of lichen partners: need and implications","author":"Verma","year":"2015"},{"key":"10.1016\/j.tiv.2020.104771_bb0250","series-title":"Lichen Secondary Metabolites","first-page":"179","article-title":"Future directions in the study of pharmaceutical potential of lichens","author":"Verma","year":"2015"},{"key":"10.1016\/j.tiv.2020.104771_bb0255","series-title":"Fact sheet","author":"World Health Organization","year":"2018"},{"key":"10.1016\/j.tiv.2020.104771_bb0260","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1248\/cpb.43.1388","article-title":"Screening of tissue culture and Thalli of lichens and some of their active constituents for inhibition of tumor promoter-induced Epstein-Barr virus activation","volume":"43","author":"Yamamoto","year":"1995","journal-title":"Chem. Pharm. Bull."},{"key":"10.1016\/j.tiv.2020.104771_bb0265","doi-asserted-by":"crossref","first-page":"778","DOI":"10.3109\/13880209.2011.633089","article-title":"Biopharmaceutical potential of lichens","volume":"50","author":"Zambare","year":"2012","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.tiv.2020.104771_bb0270","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nature08140","article-title":"The Schistosoma japonicum genome reveals features of host\u2013parasite interplay","volume":"460","author":"Zhou","year":"2009","journal-title":"Nature"}],"container-title":["Toxicology in Vitro"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233319308215?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0887233319308215?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:33:27Z","timestamp":1760135607000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0887233319308215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":54,"alternative-id":["S0887233319308215"],"URL":"https:\/\/doi.org\/10.1016\/j.tiv.2020.104771","relation":{},"ISSN":["0887-2333"],"issn-type":[{"value":"0887-2333","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Barbatic acid from Cladia aggregata (lichen): Cytotoxicity and in vitro schistosomicidal evaluation and ultrastructural analysis against adult worms of Schistosoma mansoni","name":"articletitle","label":"Article Title"},{"value":"Toxicology in Vitro","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tiv.2020.104771","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104771"}}