{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:55:41Z","timestamp":1770962141388,"version":"3.50.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology"],"published-print":{"date-parts":[[2018,1]]},"DOI":"10.1016\/j.tox.2017.10.015","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T04:04:18Z","timestamp":1509509058000},"page":"9-14","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Applicability of an Integrated Testing Strategy consisting of in silico, in chemico and in vitro assays for evaluating the skin sensitization potencies of isocyanates"],"prefix":"10.1016","volume":"393","author":[{"given":"Toshiyuki","family":"Ohtake","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosuke","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Yamanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Nakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Takeyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.tox.2017.10.015_bib0005","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.tiv.2015.09.028","article-title":"Prospective multicentre study of the U-SENS test method for skin sensitization testing","volume":"30","author":"Al\u00e9p\u00e9e","year":"2015","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0010","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0887-2333(02)00060-7","article-title":"Evaluation of CD86 expression and MHC class II molecule internalization in THP-1 human monocyte cells as predictive endpoints for contact sensitizers","volume":"16","author":"Ashikaga","year":"2002","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0015","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.tiv.2005.10.012","article-title":"Development of an in vitro skin sensitization test using human cell lines: the human cell line activation test (h-CLAT): I. optimization of the h-CLAT protocol","volume":"20","author":"Ashikaga","year":"2006","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0020","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1002\/(SICI)1099-1263(199907\/08)19:4<261::AID-JAT572>3.0.CO;2-5","article-title":"A comparison of statistical approaches to the derivation of EC3 values from local lymph node assay dose responses","volume":"19","author":"Basketter","year":"1999","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.tox.2017.10.015_bib0025","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.yrtph.2012.05.013","article-title":"Putting the parts together: combining in vitro methods to test for skin sensitizing potentials","volume":"63","author":"Bauch","year":"2012","journal-title":"Regul. Toxicol. Pharm."},{"key":"10.1016\/j.tox.2017.10.015_bib0030","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1289\/ehp.9557","article-title":"Skin exposure to isocyanates: reasons for concern","volume":"115","author":"Bello","year":"2007","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.tox.2017.10.015_bib0035","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.tiv.2015.02.012","article-title":"Genes specifically modulated in sensitized skins allow the detection of sensitizers in a reconstructed human skin model: development of the SENS-IS assay","volume":"29","author":"Cottrez","year":"2015","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0040","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.tiv.2016.01.007","article-title":"SENS-IS: a 3D reconstituted epidermis based model for quantifying chemical sensitization potency: reproducibility and predictivity results from an inter-laboratory study","volume":"32","author":"Cottrez","year":"2016","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0045","author":"European Parliament","year":"2016"},{"key":"10.1016\/j.tox.2017.10.015_bib0050","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/annweh\/wxx006","article-title":"Exposure to diisocyanates and their corresponding diamines in seven different workplaces","volume":"61","author":"Jones","year":"2017","journal-title":"Ann. Work Exposures Health"},{"issue":"2003","key":"10.1016\/j.tox.2017.10.015_bib0055","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1016\/S0278-6915(03)00223-0","article-title":"Classification of contact allergens according to potency: proposals","volume":"41","author":"Kimber","year":"2003","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.tox.2017.10.015_bib0060","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1016\/j.tiv.2015.07.006","article-title":"Optimization of the IL-8 Luc assay as an in vitro test for skin sensitization","volume":"29","author":"Kimura","year":"2015","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0065","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/j.1600-0536.2006.00969.x","article-title":"Structure-activity relationships for skin sensitization: recent improvements to Derek for Windows","volume":"55","author":"Langton","year":"2006","journal-title":"Contact Dermat."},{"key":"10.1016\/j.tox.2017.10.015_bib0070","series-title":"Act on Welfare and Management of Animals","author":"Ministry of Education, Culture, Sports, Science and Technology","year":"2005"},{"key":"10.1016\/j.tox.2017.10.015_bib0075","series-title":"Guidelines for Proper Conduct of Animal Experiments","author":"Ministry of Education, Culture, Sports, Science and Technology","year":"2006"},{"key":"10.1016\/j.tox.2017.10.015_bib0080","series-title":"OECD Guideline for Testing Chemicals","author":"OECD","year":"1992"},{"key":"10.1016\/j.tox.2017.10.015_bib0085","series-title":"OECD Guidelines for the Testing of Chemicals","author":"OECD","year":"2010"},{"key":"10.1016\/j.tox.2017.10.015_bib0090","series-title":"The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins Part 1: Scientific Evidence","author":"OECD","year":"2012"},{"key":"10.1016\/j.tox.2017.10.015_bib0095","series-title":"OECD Guidelines for the Testing of Chemicals","author":"OECD","year":"2015"},{"key":"10.1016\/j.tox.2017.10.015_bib0100","series-title":"OECD Guidelines for the Testing of Chemicals","author":"OECD","year":"2015"},{"key":"10.1016\/j.tox.2017.10.015_bib0105","series-title":"Guidance Document on the Reporting of Defined Approaches and Individual Information Sources to Be Used Within Integrated Approaches to Testing and Assessment (IATA) for Skin Sensitization","author":"OECD","year":"2016"},{"key":"10.1016\/j.tox.2017.10.015_bib0110","series-title":"Guidance Document on the Reporting of Defined Approaches to Be Used Within Integrated Approaches to Testing and Assessment","author":"OECD","year":"2016"},{"key":"10.1016\/j.tox.2017.10.015_bib0115","series-title":"OECD Guidelines for the Testing of Chemicals","author":"OECD","year":"2016"},{"key":"10.1016\/j.tox.2017.10.015_bib0120","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.tiv.2015.03.009","article-title":"The Myeloid U937 Skin Sensitization Test (U-SENS) addresses the activation of dendritic cell event in the adverse outcome pathway for skin sensitization","volume":"29","author":"Piroird","year":"2015","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0125","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1016\/j.tiv.2014.08.002","article-title":"LuSens: a keratinocyte based ARE reporter gene assay for use in integrated testing strategies for skin sensitization hazard identification","volume":"28","author":"Ramirez","year":"2014","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.tox.2017.10.015_bib0130","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1513\/pats.201002-025RM","article-title":"Skin exposure and asthma: is there a connection?","volume":"7","author":"Redlich","year":"2010","journal-title":"Proc. Am. Thorac. Soc."},{"key":"10.1016\/j.tox.2017.10.015_bib0135","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1002\/jat.3127","article-title":"Test battery with the human cell line activation test: direct peptide reactivity assay and DEREK based on a 139 chemical data set for predicting skin sensitizing potential and potency of chemicals","volume":"35","author":"Takenouchi","year":"2015","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.tox.2017.10.015_bib0140","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/jes.2011.5","article-title":"Communities near toluene diisocyanate sources: an investigation of exposure and health","volume":"21","author":"Wilder","year":"2011","journal-title":"J. Expo. Sci. Environ. Epidemiol."},{"key":"10.1016\/j.tox.2017.10.015_bib0145","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0091-6749(99)70377-5","article-title":"Isocyanate-conjugated human lung epithelial cell proteins: a link between exposure and asthma?","volume":"104","author":"Wisnewski","year":"1999","journal-title":"J. Allergy Clin. Immunol."}],"container-title":["Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300483X17303219?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300483X17303219?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T23:36:58Z","timestamp":1759534618000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300483X17303219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":29,"alternative-id":["S0300483X17303219"],"URL":"https:\/\/doi.org\/10.1016\/j.tox.2017.10.015","relation":{},"ISSN":["0300-483X"],"issn-type":[{"value":"0300-483X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Applicability of an Integrated Testing Strategy consisting of in silico, in chemico and in vitro assays for evaluating the skin sensitization potencies of isocyanates","name":"articletitle","label":"Article Title"},{"value":"Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tox.2017.10.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}