{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T11:06:16Z","timestamp":1793271976760,"version":"4.5.0"},"reference-count":106,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100007492","name":"Baylor University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.tox.2022.153281","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T01:58:16Z","timestamp":1659578296000},"page":"153281","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":61,"special_numbering":"C","title":["Comparative cytotoxicity of seven per- and polyfluoroalkyl substances (PFAS) in six human cell lines"],"prefix":"10.1016","volume":"477","author":[{"given":"Megan E.","family":"Solan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjanaa","family":"Senthilkumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace V.","family":"Aquino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica D.","family":"Bruce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Lavado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tox.2022.153281_bib1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.tiv.2005.06.043","article-title":"The effects of solvents on embryonic stem cell differentiation","volume":"20","author":"Adler","year":"2006","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2022.153116","article-title":"The impact of legacy and novel perfluoroalkyl substances on human cytochrome P450: an in vitro study on the inhibitory potential and underlying mechanisms","volume":"468","author":"Amstutz","year":"2022","journal-title":"Toxicology"},{"key":"10.1016\/j.tox.2022.153281_bib3","unstructured":"ASTR. 2018. An overview of perfluoroalkyl and polyfluoroalkyl substances and interim guidance for clinicians responding to patient exposure concerns interim guidance. Atlanta, GA, USA."},{"key":"10.1016\/j.tox.2022.153281_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2019.113061","article-title":"Sex differences in the association between perfluoroalkyl acids and liver function in US adolescents: analyses of NHANES 2013\u20132016","volume":"254","author":"Attanasio","year":"2019","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.tox.2022.153281_bib5","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1002\/tox.23004","article-title":"An assessment of serum\u2010dependent impacts on intracellular accumulation and genomic response of per\u2010 and polyfluoroalkyl substances in a placental trophoblast model","volume":"35","author":"Bangma","year":"2020","journal-title":"Environ. Toxicol."},{"key":"10.1016\/j.tox.2022.153281_bib6","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nature11003","article-title":"The cancer cell line encyclopedia enables predictive modelling of anticancer drug sensitivity","volume":"483","author":"Barretina","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.tox.2022.153281_bib7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.toxlet.2018.03.029","article-title":"Perfluoroalkylated substances (PFAS) affect neither estrogen and androgen receptor activity nor steroidogenesis in human cells in vitro","volume":"291","author":"Behr","year":"2018","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.tox.2022.153281_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2019.104700","article-title":"Activation of human nuclear receptors by perfluoroalkylated substances (PFAS)","volume":"62","author":"Behr","year":"2020","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib9","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.tox.2011.06.012","article-title":"Multiplicity of nuclear receptor activation by PFOA and PFOS in primary human and rodent hepatocytes","volume":"288","author":"Bjork","year":"2011","journal-title":"Toxicology"},{"key":"10.1016\/j.tox.2022.153281_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2019.105048","article-title":"Legacy and alternative per- and polyfluoroalkyl substances in the U.S. general population: paired serum-urine data from the 2013\u20132014 national health and nutrition examination survey","volume":"131","author":"Calafat","year":"2019","journal-title":"Environ. Int."},{"key":"10.1016\/j.tox.2022.153281_bib11","article-title":"Total and class-specific analysis of per- and polyfluoroalkyl substances in environmental samples using nuclear magnetic resonance spectroscopy","volume":"2","author":"Camdzic","year":"2021","journal-title":"J. Hazard. Mater. Lett."},{"key":"10.1016\/j.tox.2022.153281_bib12","first-page":"1623","article-title":"Absorption, distribution, and toxicity of per- and polyfluoroalkyl substances (PFAS) in the brain: a review","volume":"23","author":"Cao","year":"2021","journal-title":"Environ. Sci.: Process. Impacts"},{"issue":"Suppl 8","key":"10.1016\/j.tox.2022.153281_bib13","doi-asserted-by":"crossref","first-page":"P126","DOI":"10.1186\/1753-6561-5-S8-P126","article-title":"Optimization of HEK 293 cell growth by addition of non-animal derived components using design of experiments","volume":"5","author":"Cervera","year":"2011","journal-title":"BMC Proc."},{"key":"10.1016\/j.tox.2022.153281_bib14","doi-asserted-by":"crossref","first-page":"9930","DOI":"10.1021\/acs.est.7b02602","article-title":"A permeability-limited physiologically based pharmacokinetic (PBPK) model for perfluorooctanoic acid (PFOA) in male rats","volume":"51","author":"Cheng","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.tox.2022.153281_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.105581","volume":"137","author":"Chou","year":"2020","journal-title":"Environ. Int"},{"key":"10.1016\/j.tox.2022.153281_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.mce.2020.110922","article-title":"Perfluorooctane sulfonic acid, a persistent organic pollutant, inhibits iodide accumulation by thyroid follicular cells in vitro","volume":"515","author":"Conti","year":"2020","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.tox.2022.153281_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.152732","article-title":"Single PFAS and PFAS mixtures affect nuclear receptor- and oxidative stress-related pathways in precision-cut liver slices of Atlantic cod (Gadus morhua)","volume":"814","author":"Dale","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.tox.2022.153281_bib18","series-title":"Toxicological effects of perfluoroalkyl and polyfluoroalkyl substances","author":"DeWitt","year":"2015"},{"key":"10.1016\/j.tox.2022.153281_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2021.106982","article-title":"Impairment of human dopaminergic neurons at different developmental stages by perfluoro-octanoic acid (PFOA) and differential human brain areas accumulation of perfluoroalkyl chemicals","volume":"158","author":"Di Nisio","year":"2022","journal-title":"Environ. Int."},{"key":"10.1016\/j.tox.2022.153281_bib20","series-title":"Culture and functional characterization of human hepatoma HepG2 cells. Protocols in In Vitro Hepatocyte Research","first-page":"77","author":"Donato","year":"2015"},{"key":"10.1016\/j.tox.2022.153281_bib21","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1159\/000132446","article-title":"A specific chromosomal abnormality in rhabdomyosarcoma","volume":"45","author":"Douglass","year":"1987","journal-title":"Cytogenet. Genome Res"},{"key":"10.1016\/j.tox.2022.153281_bib22","first-page":"193","article-title":"Methods of in vitro toxicology. Food Chem. Toxicol","volume":"40","author":"Eisenbrand","year":"2002"},{"key":"10.1016\/j.tox.2022.153281_bib23","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.mrgentox.2010.04.024","article-title":"Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells","volume":"700","author":"Eriksen","year":"2010","journal-title":"Mutat. Res."},{"key":"10.1016\/j.tox.2022.153281_bib24","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1196\/annals.1329.010","article-title":"Analysis of the effects of different alcohols on MCF-7 human breast cancer cells","volume":"1030","author":"Etique","year":"2004","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.tox.2022.153281_bib25","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1002\/etc.4890","article-title":"Per\u2010 and polyfluoroalkyl substance toxicity and human health review: current state of knowledge and strategies for informing future research","volume":"40","author":"Fenton","year":"2021","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.tox.2022.153281_bib26","first-page":"539","article-title":"Persistent pollutants: focus on perfluorinated compounds and kidney","volume":"25","author":"Ferrari","year":"2019","journal-title":"Curr. Opin. Crit. Care"},{"key":"10.1016\/j.tox.2022.153281_bib27","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1093\/jnci\/58.2.209","article-title":"Absence of HeLa cell contamination in 169 cell lines derived from human tumors","volume":"58","author":"Fogh","year":"1977","journal-title":"J. Natl. Cancer Inst."},{"key":"10.1016\/j.tox.2022.153281_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.taap.2019.114623","article-title":"Developmental exposure to perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) selectively decreases brain dopamine levels in Northern leopard frogs","volume":"377","author":"Foguth","year":"2019","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tox.2022.153281_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.ntt.2020.106907","article-title":"Single and mixture per- and polyfluoroalkyl substances accumulate in developing Northern leopard frog brains and produce complex neurotransmission alterations","volume":"81","author":"Foguth","year":"2020","journal-title":"Neurotoxicol. Teratol."},{"key":"10.1016\/j.tox.2022.153281_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2019.152339","article-title":"Altered expression and activity of phase I and II biotransformation enzymes in human liver cells by perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS)","volume":"430","author":"Franco","year":"2020","journal-title":"Toxicology"},{"key":"10.1016\/j.tox.2022.153281_bib31","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/s41540-020-00166-2","article-title":"High-throughput toxicogenomic screening of chemicals in the environment using metabolically competent hepatic cell cultures","author":"Franzosa","year":"2021","journal-title":"npj Syst. Biol. Appl."},{"key":"10.1016\/j.tox.2022.153281_bib32","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/1547691X.2018.1445145","article-title":"Immunotoxic and hepatotoxic effects of perfluoro-n-decanoic acid (PFDA) on female Harlan Sprague\u2013Dawley rats and B6C3F1\/N mice when administered by oral gavage for 28 days","volume":"15","author":"Frawley","year":"2018","journal-title":"J. Immunotoxicol."},{"key":"10.1016\/j.tox.2022.153281_bib33","doi-asserted-by":"crossref","first-page":"6529","DOI":"10.1021\/acs.est.9b00715","article-title":"Prenatal exposure to per- and polyfluoroalkyl substances (PFASs) and association between the placental transfer efficiencies and dissociation constant of serum proteins\u2013PFAS complexes","volume":"53","author":"Gao","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.tox.2022.153281_bib34","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s10565-011-9208-4","article-title":"Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins","volume":"28","author":"Gerets","year":"2012","journal-title":"Cell Biol. Toxicol."},{"key":"10.1016\/j.tox.2022.153281_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.jnoncrysol.2020.120152","article-title":"Hybrid nanosilicas produced by the St\u00f6ber sol-gel process: In vitro evaluation in MRC-5 cells","volume":"542","author":"Gnoatto","year":"2020","journal-title":"J. Non-Cryst. Solids"},{"key":"10.1016\/j.tox.2022.153281_bib36","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.scitotenv.2019.04.208","article-title":"PFOA and PFOS promote diabetic renal injury in vitro by impairing the metabolisms of amino acids and purines","volume":"676","author":"Gong","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.tox.2022.153281_bib37","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.taap.2014.03.012","article-title":"Perfluorinated chemicals: differential toxicity, inhibition of aromatase activity and alteration of cellular lipids in human placental cells","volume":"277","author":"Gorrochategui","year":"2014","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tox.2022.153281_bib38","doi-asserted-by":"crossref","first-page":"3730","DOI":"10.3390\/ijerph18073730","article-title":"Prevalence and predictors of per- and polyfluoroalkyl substances (PFAS) serum levels among members of a suburban US volunteer fire department","volume":"18","author":"Graber","year":"2021","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.tox.2022.153281_bib39","doi-asserted-by":"crossref","unstructured":"Gripon, P., Rumin, S., Urban, S., Le Seyec, J., Glaise, D., Cannie, I., Guyomard, C., Lucas, J., Trepo, C. and Guguen-Guillouzo, C. 2002. Nonlinear partial differential equations and applications: Infection of a human hepatoma cell line by hepatitis B virus. Proc. Natl. Acad. Sci. U. S. A. 99, 15655\u201315660. \u3008https:\/\/doi.org\/10.1073\/pnas.232137699\u3009.","DOI":"10.1073\/pnas.232137699"},{"key":"10.1016\/j.tox.2022.153281_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.143745","article-title":"Alteration of neuro-dopamine and steroid hormone homeostasis in wild Bank voles in relation to tissue concentrations of PFAS at a Nordic skiing area","volume":"756","author":"Gr\u00f8nnestad","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.tox.2022.153281_bib41","doi-asserted-by":"crossref","first-page":"3505","DOI":"10.1007\/s00204-018-2302-0","article-title":"Relevance of the incubation period in cytotoxicity testing with primary human hepatocytes","volume":"92","author":"Gu","year":"2018","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.tox.2022.153281_bib42","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.tiv.2004.03.012","article-title":"Comparison of alamar blue and MTT assays for high through-put screening","volume":"18","author":"Hamid","year":"2004","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib43","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1016\/j.chemosphere.2017.08.070","article-title":"Perfluorooctane sulphonate induces oxidative hepatic damage via mitochondria-dependent and NF-\u03baB\/TNF-\u03b1-mediated pathway","volume":"191","author":"Han","year":"2018","journal-title":"Chemosphere"},{"key":"10.1016\/j.tox.2022.153281_bib44","doi-asserted-by":"crossref","first-page":"5193","DOI":"10.1021\/acs.est.6b05811","article-title":"Predictors of per- and polyfluoroalkyl substance (PFAS) plasma concentrations in 6\u201310 year old American children","volume":"51","author":"Harris","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.tox.2022.153281_bib45","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1038\/nprot.2007.303","article-title":"Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers","volume":"2","author":"Hubatsch","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.tox.2022.153281_bib46","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1021\/acs.est.7b04650","article-title":"Time trends in per- and polyfluoroalkyl substances (PFASs) in California women: declining serum levels, 2011\u20132015","volume":"52","author":"Hurley","year":"2018","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.tox.2022.153281_bib47","unstructured":"ICCVAM. 2006. Test method evaluation report (TMER): In vitro cytotoxicity test methods for estimating starting doses for acute oral systemic toxicity testing. InvivoGen. n.d. SEAP Reporter 293 cells expressing the human TLR2 gene."},{"key":"10.1016\/j.tox.2022.153281_bib48","doi-asserted-by":"crossref","first-page":"112","DOI":"10.3390\/toxics8040112","article-title":"Epigenetic modifications, and alterations in cell cycle and apoptosis pathway in A549 lung carcinoma cell line upon exposure to perfluoroalkyl substances","volume":"8","author":"Jabeen","year":"2020","journal-title":"Toxics"},{"key":"10.1016\/j.tox.2022.153281_bib49","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/227168a0","article-title":"Characteristics of a human diploid cell designated MRC-5","volume":"227","author":"Jacobs","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.tox.2022.153281_bib50","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1080\/19338244.2020.1732856","article-title":"Variabilities in concentrations of selected perfluoroalkyl acids among normotensives and hypertensives across various stages of glomerular function","volume":"76","author":"Jain","year":"2021","journal-title":"Arch. Environ. Occup. Health"},{"key":"10.1016\/j.tox.2022.153281_bib51","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0304-3940(94)11792-H","article-title":"Establishment of human microglial cell lines after transfection of primary cultures of embryonic microglial cells with the SV40 large T antigen","volume":"195","author":"Janabi","year":"1995","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.tox.2022.153281_bib52","series-title":"Toxicological Effects of Perfluoroalkyl and Polyfluoroalkyl Substances, Humana Press, Cham","first-page":"177","article-title":"Metabolic effects PFAS","author":"Jiang","year":"2015"},{"key":"10.1016\/j.tox.2022.153281_bib53","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.tiv.2018.08.001","article-title":"The utility of HepaRG cells for bioenergetic investigation and detection of drug-induced mitochondrial toxicity","volume":"53","author":"Kamalian","year":"2018","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib54","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.toxlet.2017.05.012","article-title":"Uptake of perfluorooctanoic acid by Caco-2 cells: involvement of organic anion transporting polypeptides","volume":"277","author":"Kimura","year":"2017","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.tox.2022.153281_bib55","article-title":"Effects of perfluoroalkyl carboxylic acids on the uptake of sulfobromophthalein via organic anion transporting polypeptides in human intestinal Caco-2 cells","volume":"24","author":"Kimura","year":"2020","journal-title":"Biochem. Biophys. Rep."},{"key":"10.1016\/j.tox.2022.153281_bib56","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.taap.2008.10.008","article-title":"Mechanism of cytotoxic action of perfluorinated acids. I. alteration in plasma membrane potential and intracellular pH level","volume":"234","author":"Kleszczynski","year":"2009","journal-title":"Toxicol. Appl. Pharm."},{"key":"10.1016\/j.tox.2022.153281_bib57","article-title":"Risk to human health related to the presence of perfluorooctane sulfonic acid and perfluorooctanoic acid in food","author":"Knutsen","year":"2018","journal-title":"EFSA J. 16"},{"key":"10.1016\/j.tox.2022.153281_bib58","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.taap.2004.06.010","article-title":"Dead or dying: the importance of time in cytotoxicity assays using arsenite as an example","volume":"202","author":"Komissarova","year":"2005","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.tox.2022.153281_bib59","doi-asserted-by":"crossref","first-page":"S92","DOI":"10.1016\/j.ibror.2019.07.299","article-title":"The human microglia (HMC-3) as a cellular model of neuroinflammation","volume":"6","author":"Lepiarz","year":"2019","journal-title":"IBRO Rep."},{"key":"10.1016\/j.tox.2022.153281_bib60","first-page":"9","article-title":"In vitro and in silico assessment of per- and polyfluoroalkyl substances (PFAS) in aqueous film-forming foam (AFFF) binding to human serum albumin","author":"Li","year":"2021","journal-title":"Toxics"},{"key":"10.1016\/j.tox.2022.153281_bib61","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1021\/acs.estlett.0c00323","article-title":"Solvent suitability for HFPO-DA (\u201cGenX\u201d Parent Acid) in toxicological studies","volume":"7","author":"Liberatore","year":"2020","journal-title":"Environ. Sci. Technol. Lett."},{"key":"10.1016\/j.tox.2022.153281_bib62","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.1038\/ncomms5767","article-title":"Genome dynamics of the human embryonic kidney 293 (HEK293) lineage in response to cell biology manipulations","volume":"5","author":"Lin","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tox.2022.153281_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.127204","article-title":"Influence of the digestive process on intestinal toxicity of polystyrene microplastics as determined by in vitro Caco-2 models","volume":"256","author":"Liu","year":"2020","journal-title":"Chemosphere"},{"key":"10.1016\/j.tox.2022.153281_bib64","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jes.2019.08.016","article-title":"The short-chain perfluorinated compounds PFBS, PFHxS, PFBA and PFHxA, disrupt human mesenchymal stem cell self-renewal and adipogenic differentiation","volume":"88","author":"Liu","year":"2020","journal-title":"J. Environ. Sci."},{"key":"10.1016\/j.tox.2022.153281_bib65","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.neures.2013.10.004","article-title":"Neuroinflammation: the role and consequences","volume":"79","author":"Lyman","year":"2014","journal-title":"Neurosci. Res."},{"key":"10.1016\/j.tox.2022.153281_bib66","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1093\/eurpub\/ckx066","article-title":"Drinking water contamination from perfluoroalkyl substances (PFAS): an ecological mortality study in the Veneto Region, Italy","volume":"28","author":"Mastrantonio","year":"2018","journal-title":"Eur. J. Public Health"},{"key":"10.1016\/j.tox.2022.153281_bib67","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/toxsci\/kft102","article-title":"Mitochondria as a target of environmental toxicants","volume":"134","author":"Meyer","year":"2013","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tox.2022.153281_bib68","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.aquatox.2006.05.009","article-title":"Dimethyl sulfoxide is a potent modulator of estrogen receptor isoforms and xenoestrogen biomarker responses in primary culture of salmon hepatocytes","volume":"79","author":"Mortensen","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.tox.2022.153281_bib69","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/toxsci\/kfn058","article-title":"Pregnane X receptor protects HepG2 cells from BaP-induced DNA damage","volume":"104","author":"Naspinski","year":"2008","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.tox.2022.153281_bib70","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.envres.2019.02.013","article-title":"Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults: isomers of C8 health project in China","volume":"172","author":"Nian","year":"2019","journal-title":"Environ. Res."},{"key":"10.1016\/j.tox.2022.153281_bib71","first-page":"1","article-title":"Guidance document on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests","volume":"20","author":"Oecd","year":"2010","journal-title":"OECD Ser. Test. Assess."},{"key":"10.1016\/j.tox.2022.153281_bib72","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1186\/1471-2407-11-211","article-title":"MDM2 antagonist Nutlin-3a potentiates antitumour activity of cytotoxic drugs in sarcoma cell lines","volume":"11","author":"Ohnstad","year":"2011","journal-title":"BMC Cancer"},{"key":"10.1016\/j.tox.2022.153281_bib73","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.114182","article-title":"Combined effects and toxicological interactions of perfluoroalkyl and polyfluoroalkyl substances mixtures in human liver cells (HepG2)","volume":"263","author":"Ojo","year":"2020","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.tox.2022.153281_bib74","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2021.130808","article-title":"Evaluation of the individual and combined toxicity of perfluoroalkyl substances to human liver cells using biomarkers of oxidative stress","volume":"281","author":"Ojo","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.tox.2022.153281_bib75","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1177\/2472555217701058","article-title":"An automated multiplexed hepatotoxicity and CYP induction assay using HepaRG cells in 2D and 3D","volume":"22","author":"Ott","year":"2017","journal-title":"SLAS Discov.: Adv. Sci. Drug Discov."},{"key":"10.1016\/j.tox.2022.153281_bib76","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1124\/mol.53.1.14","article-title":"Peroxisome proliferator activated receptor-\u03b1 expression in human liver","volume":"53","author":"Palmer","year":"1998","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.tox.2022.153281_bib77","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.envint.2013.06.004","article-title":"Accumulation of perfluoroalkyl substances in human tissues","volume":"59","author":"Perez","year":"2013","journal-title":"Environ. Int."},{"key":"10.1016\/j.tox.2022.153281_bib78","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.tox.2009.06.010","article-title":"High-dose, short-term exposure of mice to perfluorooctanesulfonate (PFOS) or perfluorooctanoate (PFOA) affects the number of circulating neutrophils differently, but enhances the inflammatory responses of macrophages to lipopolysaccharide (LPS) in a simil","volume":"262","author":"Qazi","year":"2009","journal-title":"Toxicology"},{"key":"10.1016\/j.tox.2022.153281_bib79","unstructured":"Riss, T., Niles, A., Moravec, R., Karassina, N. and Vidugiriene, J. 2019. Cytotoxicity assays: in vitro methods to measure dead cells. Assay Guidance Manual [Internet]."},{"key":"10.1016\/j.tox.2022.153281_bib80","first-page":"5407","article-title":"Amplification of the gli gene in childhood sarcomas","volume":"49","author":"Roberts","year":"1989","journal-title":"Cancer Res."},{"key":"10.1016\/j.tox.2022.153281_bib81","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3390\/livers1040017","article-title":"Perfluoroalkyl carboxylic acids interact with the human bile acid transporter NTCP","volume":"1","author":"Ruggiero","year":"2021","journal-title":"Livers"},{"key":"10.1016\/j.tox.2022.153281_bib82","doi-asserted-by":"crossref","first-page":"329","DOI":"10.18388\/abp.2008_3080","article-title":"Induction of the multixenobiotic\/multidrug resistance system in various cell lines in response to perfluorinated carboxylic acids","volume":"55","author":"Rusiecka","year":"2008","journal-title":"Acta Biochim. Pol."},{"key":"10.1016\/j.tox.2022.153281_bib83","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s00204-017-2055-1","article-title":"Cytotoxicity of novel fluorinated alternatives to long-chain perfluoroalkyl substances to human liver cell line and their binding capacity to human liver fatty acid binding protein","volume":"92","author":"Sheng","year":"2018","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.tox.2022.153281_bib84","doi-asserted-by":"crossref","first-page":"C778","DOI":"10.1152\/ajpcell.00460.2020","article-title":"Global changes to HepG2 cell metabolism in response to galactose treatment","volume":"320","author":"Skolik","year":"2021","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.tox.2022.153281_bib85","article-title":"The use of in vitro methods in assessing human health risks associated with short\u2010chain perfluoroalkyl and polyfluoroalkyl substances (PFAS)","author":"Solan","year":"2021","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.tox.2022.153281_bib86","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2019.104656","article-title":"Per- and polyfluoroalkyl substances (PFASs) modify lung surfactant function and pro-inflammatory responses in human bronchial epithelial cells","volume":"62","author":"S\u00f8rli","year":"2020","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib87","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.2215\/CJN.04670418","article-title":"Perfluorinated chemicals as emerging environmental threats to kidney health","volume":"13","author":"Stanifer","year":"2018","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"10.1016\/j.tox.2022.153281_bib88","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1289\/ehp.0901827","article-title":"Epidemiologic evidence on the health effects of perfluorooctanoic acid (PFOA)","volume":"118","author":"Steenland","year":"2010","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.tox.2022.153281_bib89","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/s41370-018-0094-1","article-title":"A review of the pathways of human exposure to poly- and perfluoroalkyl substances (PFASs) and present understanding of health effects","volume":"29","author":"Sunderland","year":"2019","journal-title":"J. Expo. Sci. Environ. Epidemiol."},{"key":"10.1016\/j.tox.2022.153281_bib90","doi-asserted-by":"crossref","first-page":"192","DOI":"10.3390\/cells8020192","article-title":"In-depth proteome analysis highlights HepaRG cells as a versatile cell system surrogate for primary human hepatocytes","volume":"8","author":"Tascher","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.tox.2022.153281_bib91","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1080\/15320383.2018.1562420","article-title":"Perfluorooctanoic acid (PFOA): environmental sources, chemistry, toxicology, and potential risks","volume":"28","author":"Teaf","year":"2019","journal-title":"Soil Sediment Contam."},{"key":"10.1016\/j.tox.2022.153281_bib92","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.vascn.2004.08.014","article-title":"HEK293 cell line: a vehicle for the expression of recombinant proteins","volume":"51","author":"Thomas","year":"2005","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"10.1016\/j.tox.2022.153281_bib93","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1007\/s10616-012-9530-6","article-title":"Considerations regarding use of solvents in in vitro cell based assays","volume":"65","author":"Timm","year":"2013","journal-title":"Cytotechnology"},{"key":"10.1016\/j.tox.2022.153281_bib94","unstructured":"USEPA. 2016. Drinking water health advisory for perfluorooctanoic acid. EPA 822-R-16\u2013005."},{"key":"10.1016\/j.tox.2022.153281_bib95","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.tiv.2010.03.016","article-title":"Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods","volume":"24","author":"van der Valk","year":"2010","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib96","doi-asserted-by":"crossref","first-page":"816","DOI":"10.3390\/ijms22020816","article-title":"Toll-like receptors in acute kidney injury","volume":"22","author":"V\u00e1zquez-Carballo","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.tox.2022.153281_bib97","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/nar\/gkaa1141","article-title":"Perfluoroalkyl substance pollutants activate the innate immune system through the AIM2 inflammasome","author":"Wang","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tox.2022.153281_bib98","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2020.104797","article-title":"Epigenetic toxicity of PFOA and GenX in HepG2 cells and their role in lipid metabolism","volume":"65","author":"Wen","year":"2020","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.tox.2022.153281_bib99","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.chemosphere.2014.10.014","article-title":"Perfluoroalkylated substances (PFAS) affect oxidative stress biomarkers in vitro","volume":"129","author":"Wiels\u00f8e","year":"2015","journal-title":"Chemosphere"},{"key":"10.1016\/j.tox.2022.153281_bib100","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/j.biomaterials.2004.04.024","article-title":"Variable cytocompatibility of six cell lines with photoinitiators used for polymerizing hydrogels and cell encapsulation","volume":"26","author":"Williams","year":"2005","journal-title":"Biomaterials"},{"key":"10.1016\/j.tox.2022.153281_bib101","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2019.105446","article-title":"Association between serum concentrations of perfluoroalkyl substances (PFAS) and expression of serum microRNAs in a cohort highly exposed to PFAS from drinking water","volume":"136","author":"Xu","year":"2020","journal-title":"Environ. Int."},{"key":"10.1016\/j.tox.2022.153281_bib102","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2019.108923","article-title":"Inflammatory bowel disease and biomarkers of gut inflammation and permeability in a community with high exposure to perfluoroalkyl substances through drinking water","volume":"181","author":"Xu","year":"2020","journal-title":"Environ. Res."},{"key":"10.1016\/j.tox.2022.153281_bib103","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.jhazmat.2014.08.043","article-title":"The role of interleukin family in perfluorooctanoic acid (PFOA)-induced immunotoxicity","volume":"280","author":"Zhang","year":"2014","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.tox.2022.153281_bib104","doi-asserted-by":"crossref","first-page":"6820","DOI":"10.1016\/j.vaccine.2014.07.071","article-title":"Human diploid MRC-5 cells exhibit several critical properties of human umbilical cord-derived mesenchymal stem cells","volume":"32","author":"Zhang","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.tox.2022.153281_bib105","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.122109","article-title":"Fetal bovine serum attenuating perfluorooctanoic acid-inducing toxicity to multiple human cell lines via albumin binding","volume":"389","author":"Zhang","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.tox.2022.153281_bib106","doi-asserted-by":"crossref","DOI":"10.1063\/1.4979104","article-title":"A549 and MRC-5 cell aggregation in a microfluidic -Lab-on-a-chip- system","volume":"11","author":"Zuchowska","year":"2017","journal-title":"Biomicrofluidics"}],"container-title":["Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300483X22001937?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300483X22001937?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T23:52:56Z","timestamp":1759535576000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300483X22001937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":106,"alternative-id":["S0300483X22001937"],"URL":"https:\/\/doi.org\/10.1016\/j.tox.2022.153281","relation":{},"ISSN":["0300-483X"],"issn-type":[{"value":"0300-483X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative cytotoxicity of seven per- and polyfluoroalkyl substances (PFAS) in six human cell lines","name":"articletitle","label":"Article Title"},{"value":"Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tox.2022.153281","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"153281"}}