{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:18:24Z","timestamp":1790871504814,"version":"4.1.0"},"reference-count":65,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:00:00Z","timestamp":1741996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008736","name":"Fondo de Fomento al Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["ID20I10056"],"award-info":[{"award-number":["ID20I10056"]}],"id":[{"id":"10.13039\/501100008736","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013398","name":"Office of International Studies and Programs, University of Missouri, St. Louis","doi-asserted-by":"publisher","award":["FONDEF ID20I10056"],"award-info":[{"award-number":["FONDEF ID20I10056"]}],"id":[{"id":"10.13039\/100013398","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon: X"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.toxcx.2025.100222","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T15:08:13Z","timestamp":1743088093000},"page":"100222","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Predictive analysis of B-cell antigenic epitopes in phospholipase D toxins from Loxosceles spiders"],"prefix":"10.1016","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6886-2489","authenticated-orcid":false,"given":"Alejandro","family":"Catal\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Sambra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Cadena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s","family":"Ar\u00e1n-Sekul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"San Francisco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"V\u00e1squez-Saez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abel","family":"V\u00e1squez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge E.","family":"Araya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxcx.2025.100222_bib1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1745-7580-6-6","article-title":"Identification of conformational B-cell Epitopes in an antigen from its primary sequence","volume":"6","author":"Ansari","year":"2010","journal-title":"Immunome Res."},{"key":"10.1016\/j.toxcx.2025.100222_bib2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0041-0101(94)90027-2","article-title":"Antigenic cross-reactivity of venoms from medically important Loxosceles (Araneae) species in Brazil","volume":"32","author":"Barbaro","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib3","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.toxicon.2004.12.009","article-title":"Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas","volume":"45","author":"Barbaro","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib4","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1093\/molbev\/msn274","article-title":"Molecular evolution, functional variation, and proposed nomenclature of the gene family that includes sphingomyelinase D in sicariid spider venoms","volume":"26","author":"Binford","year":"2009","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.toxcx.2025.100222_bib5","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.ympev.2008.08.003","article-title":"Phylogenetic relationships of Loxosceles and Sicarius spiders are consistent with Western Gondwanan vicariance","volume":"49","author":"Binford","year":"2008","journal-title":"Mol. Phylogenet. Evol."},{"key":"10.1016\/j.toxcx.2025.100222_bib6","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.toxicon.2015.09.041","article-title":"Brown spider (Loxosceles genus) venom toxins: evaluation of biological conservation by immune cross-reactivity","volume":"108","author":"Buch","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib7","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1002\/jbt.20399","article-title":"Two new phospholipase D isoforms of Loxosceles laeta: cloning, heterologous expression, functional characterization, and potential biotechnological application","volume":"25","author":"Catalan","year":"2011","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"10.1016\/j.toxcx.2025.100222_bib8","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.taap.2005.05.015","article-title":"Brown spider dermonecrotic toxin directly induces nephrotoxicity","volume":"211","author":"Chaim","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.toxcx.2025.100222_bib9","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.bbalip.2010.11.005","article-title":"Phospholipase-D activity and inflammatory response induced by brown spider dermonecrotic toxin: endothelial cell membrane phospholipids as targets for toxicity","volume":"1811","author":"Chaim","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxcx.2025.100222_bib10","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.toxicon.2004.05.028","article-title":"Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Loxosceles intermedia","volume":"44","author":"de Castro","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241512006","article-title":"Comparative biochemical, structural, and functional analysis of recombinant phospholipases D from three Loxosceles spider venoms","volume":"24","author":"da Justa","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.toxcx.2025.100222_bib12","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0041-0101(02)00078-8","article-title":"Identification of proteases in the extract of venom glands from brown spiders","volume":"40","author":"da Silveira","year":"2002","journal-title":"Toxicon"},{"issue":"6","key":"10.1016\/j.toxcx.2025.100222_bib13","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.toxicon.2006.11.024","article-title":"Hyaluronidases in Loxosceles intermedia (Brown spider) venom are endo-beta-N-acetyl-d-hexosaminidases hydrolases","volume":"49","author":"da Silveira","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib14","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.toxicon.2016.08.002","article-title":"Description of Loxtox protein family and identification of a new group of Phospholipases D from Loxosceles similis venom gland","volume":"120","author":"Dantas","year":"2016","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib15","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.bbrc.2011.05.053","article-title":"Structure of a novel class II phospholipase D: catalytic cleft is modified by a disulphide bridge","volume":"409","author":"de Giuseppe","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxcx.2025.100222_bib16","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.toxicon.2009.09.019","article-title":"A protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptide","volume":"55","author":"Dias-Lopes","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.toxicon.2014.10.023","article-title":"Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru","volume":"93","author":"Duarte","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib18","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0041-0101(97)00083-4","article-title":"Detection and characterization of metalloproteinases with gelatinolytic, fibronectinolytic and fibrinogenolytic activities in brown spider (Loxosceles intermedia) venom","volume":"36","author":"Feitosa","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib19","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.toxicon.2006.06.019","article-title":"Functional characterization and epitope analysis of a recombinant dermonecrotic protein from Loxosceles intermedia spider","volume":"48","author":"Felicori","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib20","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1016\/j.vaccine.2009.04.038","article-title":"An in vivo protective response against toxic effects of the dermonecrotic protein from Loxosceles intermedia spider venom elicited by synthetic epitopes","volume":"27","author":"Felicori","year":"2009","journal-title":"Vaccine"},{"issue":"5","key":"10.1016\/j.toxcx.2025.100222_bib21","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/S0006-291X(02)02521-4","article-title":"Molecular cloning and expression of a functional dermonecrotic and haemolytic factor from Loxosceles laeta venom","volume":"298","author":"Fernandes","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxcx.2025.100222_bib22","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1186\/1471-2164-9-279","article-title":"Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags","volume":"9","author":"Fernandes-Pedrosa Mde","year":"2008","journal-title":"BMC Genom."},{"key":"10.1016\/j.toxcx.2025.100222_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxcx.2020.100036","article-title":"Biotechnological potential of Phospholipase D for Loxosceles antivenom development","volume":"6","author":"Fingermann","year":"2020","journal-title":"Toxicon X"},{"key":"10.1016\/j.toxcx.2025.100222_bib24","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/0003-9861(78)90046-2","article-title":"Red blood cell lysis induced by the venom of the brown recluse spider. The role of sphingomyelinase D","volume":"187","author":"Forrester","year":"1978","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxcx.2025.100222_bib25","article-title":"Purification and partial characterization of sphingomyelinase-D from Loxosceles reclusa venom","volume":"40","author":"Forrester","year":"1981","journal-title":"Fed. Proc."},{"key":"10.1016\/j.toxcx.2025.100222_bib26","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/S0041-0101(00)00212-9","article-title":"Antigenic cross-reactivity of venoms from medically important North American Loxosceles spider species","volume":"39","author":"Gomez","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/toxins12030164","article-title":"Forty years of the description of Brown spider venom phospholipases-D","volume":"12","author":"Gremski","year":"2020","journal-title":"Toxins"},{"key":"10.1016\/j.toxcx.2025.100222_bib28","doi-asserted-by":"crossref","DOI":"10.3390\/toxins15010017","article-title":"Systemic loxoscelism, less frequent but more deadly: the involvement of phospholipases D in the pathophysiology of envenomation","volume":"15","author":"Gremski","year":"2022","journal-title":"Toxins"},{"key":"10.1016\/j.toxcx.2025.100222_bib29","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.1039\/c004118a","article-title":"A novel expression profile of the Loxosceles intermedia spider venomous gland revealed by transcriptome analysis","volume":"6","author":"Gremski","year":"2010","journal-title":"Mol. Biosyst."},{"key":"10.1016\/j.toxcx.2025.100222_bib30","doi-asserted-by":"crossref","DOI":"10.1590\/1678-9199-jvatitd-2020-0188","article-title":"Brown spider venom toxins: what are the functions of astacins, serine proteases, hyaluronidases, allergens, TCTP, serpins and knottins?","volume":"27","author":"Gremski","year":"2021","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.toxcx.2025.100222_bib31","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2021.706704","article-title":"Prospective use of Brown spider venom toxins as therapeutic and biotechnological inputs","volume":"8","author":"Gremski","year":"2021","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.toxcx.2025.100222_bib32","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1002\/elps.1150181505","article-title":"SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling","volume":"18","author":"Guex","year":"1997","journal-title":"Electrophoresis"},{"key":"10.1016\/j.toxcx.2025.100222_bib33","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1016\/S0041-0101(01)00085-X","article-title":"Neutralization of dermonecrotic and lethal activities and differences among 32-35 kDa toxins of medically important Loxosceles spider venoms in Brazil revealed by monoclonal antibodies","volume":"39","author":"Guilherme","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib34","doi-asserted-by":"crossref","first-page":"W24","DOI":"10.1093\/nar\/gkx346","article-title":"BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"Jespersen","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.toxcx.2025.100222_bib35","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1016\/S0041-0101(02)00201-5","article-title":"Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom","volume":"40","author":"Kalapothakis","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib36","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.toxicon.2007.07.001","article-title":"The Loxtox protein family in Loxosceles intermedia (Mello-Leitao) venom","volume":"50","author":"Kalapothakis","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib37","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1016\/j.biochi.2008.07.011","article-title":"Nephrotoxicity caused by brown spider venom phospholipase-D (dermonecrotic toxin) depends on catalytic activity","volume":"90","author":"Kusma","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.toxcx.2025.100222_bib38","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"Mega X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.toxcx.2025.100222_bib39","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/0304-4165(81)90128-8","article-title":"Platelet aggregation and sphingomyelinase D activity of a purified toxin from the venom of Loxosceles reclusa","volume":"678","author":"Kurpiewski","year":"1981","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxcx.2025.100222_bib40","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0072372","article-title":"Phospholipase D toxins of brown spider venom convert lysophosphatidylcholine and sphingomyelin to cyclic phosphates","volume":"8","author":"Lajoie","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.toxcx.2025.100222_bib41","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1042\/BJ20050043","article-title":"Brown recluse spider (Loxosceles reclusa) venom phospholipase D (PLD) generates lysophosphatidic acid (LPA)","volume":"391","author":"Lee","year":"2005","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxcx.2025.100222_bib42","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2018.00653","article-title":"Recombinant protein containing B-cell epitopes of different spider toxins generates neutralizing antibodies in immunized rabbits","volume":"9","author":"Lima","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.toxcx.2025.100222_bib43","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1002\/pmic.200401096","article-title":"Proteome analysis of brown spider venom: identification of loxnecrogin isoforms in Loxosceles gaucho venom","volume":"5","author":"Machado","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.toxcx.2025.100222_bib44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.toxicon.2013.05.014","article-title":"A novel ICK peptide from the Loxosceles intermedia (brown spider) venom gland: cloning, heterologous expression and immunological cross-reactivity approaches","volume":"71","author":"Matsubara","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib45","article-title":"Analysis of NGS data from Peruvian Loxosceles laeta spider venom gland reveals toxin diversity","volume":"43","author":"Medina-Santos","year":"2022","journal-title":"Comp. Biochem. Physiol., Part D: Genomics Proteomics"},{"key":"10.1016\/j.toxcx.2025.100222_bib46","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.vaccine.2013.03.048","article-title":"Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from spider venom","volume":"31","author":"Mendes","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.toxcx.2025.100222_bib47","doi-asserted-by":"crossref","first-page":"13658","DOI":"10.1074\/jbc.M412437200","article-title":"Structural basis for metal ion coordination and the catalytic mechanism of sphingomyelinases D","volume":"280","author":"Murakami","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxcx.2025.100222_bib48","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1002\/jmr.602","article-title":"BEPITOPE: predicting the location of continuous epitopes and patterns in proteins","volume":"16","author":"Odorico","year":"2003","journal-title":"J Mol Recognit"},{"key":"10.1016\/j.toxcx.2025.100222_bib49","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.1016\/j.vaccine.2016.05.039","article-title":"Protective antibodies against a sphingomyelinase D from Loxosceles intermedia spider venom elicited in mice with different genetic background","volume":"34","author":"Oliveira","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.toxcx.2025.100222_bib50","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.toxicon.2006.04.010","article-title":"North and South American Loxosceles spiders: development of a polyvalent antivenom with recombinant sphingomyelinases D as antigens","volume":"48","author":"Olvera","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib51","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.ijbiomac.2021.10.005","article-title":"A protective vaccine against the toxic activities following Brown spider accidents based on recombinant mutated phospholipases D as antigens","volume":"192","author":"Polli","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.toxcx.2025.100222_bib52","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1186\/1471-2105-9-514","article-title":"ElliPro: a new structure-based tool for the prediction of antibody epitopes","volume":"9","author":"Ponomarenko","year":"2008","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.toxcx.2025.100222_bib53","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.toxicon.2013.07.007","article-title":"Synthetic peptides for in vitro evaluation of the neutralizing potency of Loxosceles antivenoms","volume":"73","author":"Ramada","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib54","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1016\/j.toxicon.2007.08.001","article-title":"Biological and structural comparison of recombinant phospholipase D toxins from Loxosceles intermedia (brown spider) venom","volume":"50","author":"Ribeiro","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxcx.2025.100222_bib55","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B-cell epitopes in an antigen using recurrent neural network","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"10.1016\/j.toxcx.2025.100222_bib56","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.biotechadv.2007.12.003","article-title":"Biotechnological applications of brown spider (Loxosceles genus) venom toxins","volume":"26","author":"Senff-Ribeiro","year":"2008","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.toxcx.2025.100222_bib57","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.toxcx.2025.100222_bib58","doi-asserted-by":"crossref","first-page":"7324","DOI":"10.1016\/j.vaccine.2018.10.035","article-title":"Immunoprotection elicited in rabbit by a chimeric protein containing B-cell epitopes of Sphingomyelinases D from Loxosceles spp. spiders","volume":"36","author":"Souza","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.toxcx.2025.100222_bib59","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1038\/nbt.3988","article-title":"MMseqs2 enables sensitive protein sequence searching for the analysis of massive data sets","volume":"35","author":"Steinegger","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.toxcx.2025.100222_bib60","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1006\/bbrc.1998.9474","article-title":"Sphingomyelinases in the venom of the spider Loxosceles intermedia are responsible for both dermonecrosis and complement-dependent hemolysis","volume":"251","author":"Tambourgi","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"Part 4","key":"10.1016\/j.toxcx.2025.100222_bib61","article-title":"Subtranscriptome analysis of phospholipases D in Loxosceles venom glands: confirmation of predominance, intra-species diversity, and description of novel isoforms","volume":"280","author":"Theodoro","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.toxcx.2025.100222_bib62","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","article-title":"Jalview Version 2--a multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.toxcx.2025.100222_bib63","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1093\/oxfordjournals.molbev.a003851","article-title":"A general empirical model of protein evolution derived from multiple protein families using a maximum-likelihood approach","volume":"18","author":"Whelan","year":"2001","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.toxcx.2025.100222_bib64","article-title":"World spider catalog. Version 26","year":"2025","journal-title":"Natural History Museum Bern"},{"key":"10.1016\/j.toxcx.2025.100222_bib65","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.jmb.2017.12.007","article-title":"A completely reimplemented MPI bioinformatics toolkit with a new HHpred server at its core","volume":"430","author":"Zimmermann","year":"2018","journal-title":"J. Mol. Biol."}],"container-title":["Toxicon: X"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590171025000098?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590171025000098?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T20:53:04Z","timestamp":1762635184000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2590171025000098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":65,"alternative-id":["S2590171025000098"],"URL":"https:\/\/doi.org\/10.1016\/j.toxcx.2025.100222","relation":{},"ISSN":["2590-1710"],"issn-type":[{"value":"2590-1710","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Predictive analysis of B-cell antigenic epitopes in phospholipase D toxins from Loxosceles spiders","name":"articletitle","label":"Article Title"},{"value":"Toxicon: X","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxcx.2025.100222","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100222"}}