{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:31:01Z","timestamp":1791484261420,"version":"4.3.4"},"reference-count":31,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2004,8]]},"DOI":"10.1016\/j.toxicon.2004.05.012","type":"journal-article","created":{"date-parts":[[2004,6,21]],"date-time":"2004-06-21T19:11:00Z","timestamp":1087845060000},"page":"149-156","source":"Crossref","is-referenced-by-count":31,"title":["Rapid and efficient identification of cysteine-rich peptides by random screening of a venom gland cDNA library from the hexathelid spider Macrothele gigas"],"prefix":"10.1016","volume":"44","author":[{"given":"H","family":"Satake","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Villegas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Oshiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Terada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Shinada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Corzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2004.05.012_BIB1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/BF00235695","article-title":"Pyroglutamic acid. Non-metabolic formation, function in proteins and peptides, and characteristics of the enzymes effecting its removal","volume":"38","author":"Abraham","year":"1981","journal-title":"Mol. Cell Biochem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0006-3495(01)75681-0","article-title":"Interaction of SNX482 with domains III and IV inhibits activation gating of alpha(1E) (Ca(V)2.3) calcium channels","volume":"81","author":"Bourinet","year":"2001","journal-title":"Biophys. J."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB3","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1038\/298686a0","article-title":"Mechanism of C-terminal amide formation by pituitary enzymes","volume":"298","author":"Bradbury","year":"1982","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB4","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1042\/bj2500401","article-title":"Amino acid sequence of versutoxin, a lethal neurotoxin from the venom of the funnel-web spider Atrax versutus","volume":"250","author":"Brown","year":"1988","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB5","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/S0041-0101(03)00011-4","article-title":"Molecular cloning and characterization of Phoneutria nigriventer toxins active on calcium channels","volume":"41","author":"Cardoso","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB6","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1007\/s00018-003-3108-6","article-title":"Pharmacologically active spider peptide toxins","volume":"60","author":"Corzo","year":"2003","journal-title":"Cell Mol. Life Sci."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB7","doi-asserted-by":"crossref","first-page":"5783","DOI":"10.1046\/j.1432-1327.2000.01653.x","article-title":"Isolation, synthesis and pharmacological characterization of delta-palutoxins IT, novel insecticidal toxins from the spider Paracoelotes luctuosus","volume":"267","author":"Corzo","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB8","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0014-5793(03)00666-5","article-title":"Distinct primary structures of the major peptide toxins from the venom of the spider Macrothele gigas that bind to sites 3 and 4 in the sodium channel","volume":"547","author":"Corzo","year":"2003","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB9","doi-asserted-by":"crossref","first-page":"15340","DOI":"10.1016\/S0021-9258(18)82260-8","article-title":"Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian \u201carmed\u201d spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin","volume":"268","author":"Diniz","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB10","doi-asserted-by":"crossref","first-page":"7535","DOI":"10.1074\/jbc.271.13.7535","article-title":"Cloning and structure of delta-latroinsectotoxin, a novel insect-specific member of the latrotoxin family: functional expression requires C-terminal truncation","volume":"271","author":"Dulubova","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB11","doi-asserted-by":"crossref","first-page":"7827","DOI":"10.1016\/S0021-9258(20)89524-6","article-title":"Peptidyl-alpha-hydroxyglycine alpha-amidating lyase. Purification, characterization, and expression","volume":"266","author":"Eipper","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB12","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0300-9084(00)01166-4","article-title":"Structure and pharmacology of spider venom neurotoxins","volume":"82","author":"Escoubas","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/978-3-0348-8183-8_4","article-title":"Potassium channels: gene family, therapeutic relevance, high-throughput screening technologies and drug discovery","volume":"58","author":"Ford","year":"2002","journal-title":"Prog. Drug Res."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB14","doi-asserted-by":"crossref","first-page":"5769","DOI":"10.1074\/jbc.274.9.5769","article-title":"The putative bioactive surface of insect-selective scorpion excitatory neurotoxins","volume":"274","author":"Froy","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB15","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1016\/S0041-0101(98)00127-5","article-title":"Cloning, cDNA sequence analysis and patch clamp studies of a toxin from the venom of the armed spider (Phoneutria nigriventer)","volume":"36","author":"Kalapothakis","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB16","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1016\/0965-1748(95)00029-U","article-title":"Characterization and cloning of insecticidal peptides from the primitive weaving spider Diguetia canities","volume":"25","author":"Krapcho","year":"1995","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB17","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-2836(82)90515-0","article-title":"A simple method for displaying the hydropathic character of a protein","volume":"157","author":"Kyte","year":"1982","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB18","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/S0014-5793(98)01378-7","article-title":"Delta-atracotoxins from Australian funnel-web spiders compete with scorpion alpha-toxin binding on both rat brain and insect sodium channels","volume":"439","author":"Little","year":"1998","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB19","doi-asserted-by":"crossref","first-page":"27076","DOI":"10.1074\/jbc.273.42.27076","article-title":"delta-Atracotoxins from australian funnel-web spiders compete with scorpion alpha-toxin binding but differentially modulate alkaloid toxin activation of voltage-gated sodium channels","volume":"273","author":"Little","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB20","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0014-5793(02)02988-5","article-title":"Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2-6","volume":"523","author":"Matavel","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB21","doi-asserted-by":"crossref","first-page":"14734","DOI":"10.1021\/bi026546a","article-title":"Two tarantula peptides inhibit activation of multiple sodium channels","volume":"41","author":"Middleton","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB22","doi-asserted-by":"crossref","first-page":"43033","DOI":"10.1074\/jbc.M206690200","article-title":"Structure of a novel P-superfamily spasmodic conotoxin reveals an inhibitory cystine knot motif","volume":"277","author":"Miles","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB23","doi-asserted-by":"crossref","first-page":"15353","DOI":"10.1021\/bi981255g","article-title":"Selective peptide antagonist of the class E calcium channel from the venom of the tarantula Hysterocrates gigas","volume":"37","author":"Newcomb","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB24","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/S0041-0101(98)00149-4","article-title":"The cystine knot structure of ion channel toxins and related polypeptides","volume":"36","author":"Norton","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB25","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/S0041-0101(99)00237-8","article-title":"Molecular cloning of cDNAs encoding insecticidal neurotoxic peptides from the spider Phoneutria nigriventer","volume":"38","author":"Penaforte","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB26","doi-asserted-by":"crossref","first-page":"47564","DOI":"10.1074\/jbc.M204063200","article-title":"Function and solution structure of huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena","volume":"277","author":"Peng","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB27","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/0014-5793(85)81132-7","article-title":"Complete amino acid sequence of a new type of lethal neurotoxin from the venom of the funnel-web spider Atrax robustus","volume":"181","author":"Sheumack","year":"1985","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB28","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/0165-9936(86)80045-0","article-title":"Calculation of the isoelectric points of polypeptides from the amino acid composition","volume":"5","author":"Skoog","year":"1986","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB29","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/0896-6273(95)90184-1","article-title":"An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantula","volume":"15","author":"Swartz","year":"1995","journal-title":"Neuron"},{"key":"10.1016\/j.toxicon.2004.05.012_BIB30","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0014-5793(00)01339-9","article-title":"Isolation and pharmacological characterisation of delta-atracotoxin-Hv1b, a vertebrate-selective sodium channel toxin","volume":"470","author":"Szeto","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.05.012_BIB31","doi-asserted-by":"crossref","first-page":"14810","DOI":"10.1074\/jbc.272.23.14810","article-title":"Identification of structural elements of a scorpion alpha-neurotoxin important for receptor site recognition","volume":"272","author":"Zilberberg","year":"1997","journal-title":"J. Biol. Chem."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010104002119?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010104002119?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T15:05:29Z","timestamp":1624460729000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010104002119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["S0041010104002119"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2004.05.012","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2004,8]]}}}