{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:23:15Z","timestamp":1789935795564,"version":"4.0.1"},"reference-count":42,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2005,3]]},"DOI":"10.1016\/j.toxicon.2004.10.014","type":"journal-article","created":{"date-parts":[[2004,12,12]],"date-time":"2004-12-12T00:47:11Z","timestamp":1102812431000},"page":"273-283","source":"Crossref","is-referenced-by-count":92,"title":["The Brazilian scorpion Tityus costatus Karsch: genes, peptides and function"],"prefix":"10.1016","volume":"45","author":[{"given":"Elia","family":"Diego-Garc\u00eda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar V.F.","family":"Batista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca I.","family":"Garc\u00eda-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Lucas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise M.","family":"Candido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Froylan","family":"G\u00f3mez-Lagunas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2004.10.014_bib1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jchromb.2003.09.002","article-title":"Proteomics of the venom from the Amazonian scorpion Tityus cambridgei and the role of prolines on mass spectrometry analysis","volume":"803","author":"Batista","year":"2004","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.toxicon.2004.10.014_bib2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S1570-9639(02)00458-2","article-title":"Two novel toxins from the Amazonian scorpion Tityus cambridgei that block Kv1.3 and Shaker B K+-channels with distinctly different affinities","volume":"1601","author":"Batista","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2004.10.014_bib3","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1042\/bj3130753","article-title":"Toxic peptides and genes encoding gamma toxin of the Brazilian scorpions Tityus bahiensis and Tityus stigmurus","volume":"313","author":"Becerril","year":"1996","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxicon.2004.10.014_bib4","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/S0041-0101(96)00198-5","article-title":"Toxins and genes isolated from scorpions of the genus Tityus: a review","volume":"35","author":"Becerril","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.10.014_bib5","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1016\/0006-291X(84)91211-7","article-title":"Amino acid sequence of toxin VII, a beta-toxin from the venom of the scorpion Tityus serrulatus","volume":"122","author":"Bechis","year":"1984","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2004.10.014_bib6","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0003-2697(92)90013-W","article-title":"Alkylation of cysteine with acrylamide for protein sequence analysis","volume":"207","author":"Brune","year":"1992","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2004.10.014_bib7","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0041-0101(96)00133-X","article-title":"Neutralizing capacity of antibodies elicited by a non-toxic protein purified from the venom of the scorpion Tityus serrulatus","volume":"35","author":"Chavez-Olortegui","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.10.014_bib8","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/S0161-5890(01)00117-1","article-title":"Molecular basis for the cross-reactivity of antibodies elicited by a natural anatoxin with \u03b1- and beta-toxins from the venom of Tityus serrulatus scorpion","volume":"38","author":"Chavez-Olortegui","year":"2002","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.toxicon.2004.10.014_bib9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0014-5793(00)01384-3","article-title":"Scorpine, an anti-malaria and anti-bacterial agent purified from scorpion venom","volume":"471","author":"Conde","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.10.014_bib10","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0041-0101(02)00247-7","article-title":"Disulfide bridges and blockage of Shaker B K+-channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus","volume":"41","author":"Coronas","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.10.014_bib11","doi-asserted-by":"crossref","first-page":"4302","DOI":"10.1074\/jbc.272.7.4302","article-title":"On the convergent evolution of animal toxins. Conservation of a diad of functional residues in potassium channel-blocking toxins with unrelated structures","volume":"272","author":"Dauplais","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.10.014_bib12","doi-asserted-by":"crossref","first-page":"457","DOI":"10.3109\/15569549509019474","article-title":"The toxins purified from Tityus serrulatus (Lutz and Mello) venom","volume":"14","author":"de Lima","year":"1995","journal-title":"J. Toxicol. Toxin Rev."},{"key":"10.1016\/j.toxicon.2004.10.014_bib13","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/PL00006457","article-title":"Dynamic diversification from a putative common ancestor of scorpion toxin affecting potassium, sodium and chloride channels","volume":"48","author":"Froy","year":"1999","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.toxicon.2004.10.014_bib14","series-title":"Perspectives in Molecular Toxinology","first-page":"215","article-title":"Scorpion toxins differentiating among neuronal sodium channel subtypes: nature's guide for design of selective drugs","author":"Gordon","year":"2002"},{"key":"10.1016\/j.toxicon.2004.10.014_bib15","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/S0041-0101(02)00142-3","article-title":"An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch","volume":"40","author":"Goudet","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.10.014_bib16","series-title":"Perspectives in Molecular Toxinology","first-page":"239","article-title":"Diversification of toxic sites on a conserved protein scaffold: a scorpion recipe for survival","author":"Gurevitz","year":"2002"},{"key":"10.1016\/j.toxicon.2004.10.014_bib17","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1006\/abbi.2000.1858","article-title":"NMR solution structure of butantoxin","volume":"379","author":"Holaday","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2004.10.014_bib18","first-page":"97","article-title":"Scorpionologische Beitr\u00e4ge. II","volume":"3","author":"Karsch","year":"1879","journal-title":"Mitt. M\u00fcnch. Ent. Ver."},{"key":"10.1016\/j.toxicon.2004.10.014_bib19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1085\/jgp.93.1.67","article-title":"Modification of Na+ channel gating by an alpha scorpion toxin from Tityus serrulatus","volume":"93","author":"Kirsh","year":"1989","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.toxicon.2004.10.014_bib20","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/0896-6273(90)90311-3","article-title":"Functional expression of Shaker K+ channels in a baculovirus-infected insect cell line","volume":"5","author":"Klaiber","year":"1990","journal-title":"Neuron"},{"key":"10.1016\/j.toxicon.2004.10.014_bib21","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0014-5793(96)00616-3","article-title":"Characterization of a new peptide from Tityus serrulatus scorpion venom which is a ligand of the apamin-binding site","volume":"390","author":"Legros","year":"1996","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.10.014_bib22","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1111\/j.1432-1033.1984.tb08241.x","article-title":"Characterization, solubilization, affinity labeling and purification of the cardiac Na+ channel using Tityus toxin gamma","volume":"141","author":"Lombet","year":"1984","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2004.10.014_bib23","unstructured":"Louren\u00e7o, W.R., 2002. Scorpions of Brazil. Les \u00c9ditions de l'If. Paris, 307 pp."},{"key":"10.1016\/j.toxicon.2004.10.014_bib24","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/BF01025013","article-title":"The complete amino acid sequence of toxin TsTX-VI isolated from the venom of the scorpion Tityus serrulatus","volume":"9","author":"Marangoni","year":"1990","journal-title":"J. Prot. Chem."},{"key":"10.1016\/j.toxicon.2004.10.014_bib26","series-title":"Handbook on Neurotoxicology","first-page":"683","article-title":"Scorpion neurotoxins: effects and mechanisms","author":"Martin-Eauclaire","year":"1995"},{"key":"10.1016\/j.toxicon.2004.10.014_bib25","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/0014-5793(92)80445-M","article-title":"Molecular cloning and nucleotide sequence analysis of the cDNA encoding the main \u03b2-neurotoxin from the venom of the South American scorpion Tityus serrulatus","volume":"302","author":"Martin-Eauclaire","year":"1992","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2004.10.014_bib27","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1002\/rcm.415","article-title":"Purification, amino-acid sequence and partial characterization of two toxins with anti-insect activity from the venom of the South American scorpion Tityus bahiensis (Buthidae)","volume":"15","author":"Pimenta","year":"2001","journal-title":"Rapid. Commun. Mass Spectrom."},{"key":"10.1016\/j.toxicon.2004.10.014_bib28","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/jmbi.1999.2868","article-title":"Crystal structure of neurotoxin Ts1 from Tityus serrulatus provides insights into the specificity and toxicity of scorpion toxins","volume":"290","author":"Polikarpov","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2004.10.014_bib31","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/BF02906497","article-title":"Purification and chemical characterization of the major toxins from the venom of the Brazilian scorpion Tityus serrulatus Lutz and Mello","volume":"46","author":"Possani","year":"1981","journal-title":"Carlsberg Res. Commun."},{"key":"10.1016\/j.toxicon.2004.10.014_bib32","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/BF02907789","article-title":"The primary structure of noxiustoxin: a K+ channel blocking peptide purified from the venom of the scorpion Centruroides noxius","volume":"47","author":"Possani","year":"1982","journal-title":"Carlsberg Res. Comm."},{"key":"10.1016\/j.toxicon.2004.10.014_bib30","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1016\/S0021-9258(18)49971-1","article-title":"Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom","volume":"266","author":"Possani","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2004.10.014_bib29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1046\/j.1432-1327.1999.00625.x","article-title":"Scorpion toxins specific for Na+-channels","volume":"264","author":"Possani","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2004.10.014_bib33","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/S0300-9084(00)01167-6","article-title":"Peptides and genes coding for scorpion toxins that affect ion-channels","volume":"82","author":"Possani","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2004.10.014_bib34","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.toxicon.2004.03.022","article-title":"Current views on scorpion toxins specific for K+-channels","volume":"43","author":"Rodr\u00edguez de la Vega","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2004.10.014_bib35","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1073\/pnas.91.4.1475","article-title":"Tityustoxin K alpha blocks voltage-gated noninactivating K+ channels and unblocks inactivating K+ channels blocked by alpha-dendrotoxin in synaptosomes","volume":"91","author":"Rogowski","year":"1994","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.toxicon.2004.10.014_bib36","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"Thompson","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.toxicon.2004.10.014_bib37","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/S0165-6147(99)01398-X","article-title":"A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies","volume":"20","author":"Tytgat","year":"1999","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.toxicon.2004.10.014_bib38","first-page":"247","article-title":"A novel class of pore-forming peptides in the venom of Parabuthus schlechteri Purcell (Scorpions: Buthidae)","volume":"16","author":"Verdonck","year":"2000","journal-title":"Cimbebasia"},{"key":"10.1016\/j.toxicon.2004.10.014_bib39","first-page":"H443","article-title":"Effects of two new world scorpion toxins on single channel and whole cell cardiac sodium channels","volume":"254","author":"Yatani","year":"1988","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.toxicon.2004.10.014_bib40","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.peptides.2003.12.003","article-title":"Identification and functional characterization of novel scorpion venom peptides with no disulfide bridge from Buthus martensii Karsch","volume":"25","author":"Zeng","year":"2004","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2004.10.014_bib42","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1007\/s00018-004-4149-1","article-title":"The scorpine family of defensins: gene structure, alternative polyadenylation and fold recognition","volume":"61","author":"Zhu","year":"2004","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.toxicon.2004.10.014_bib41","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0014-5793(99)01101-1","article-title":"Molecular cloning and sequencing of two short chain and two long chain K(+) channel-blocking peptides from the Chinese scorpion Buthus martensii Karsch","volume":"457","author":"Zhu","year":"1999","journal-title":"FEBS Lett."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010104004453?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010104004453?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T04:09:55Z","timestamp":1625112595000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010104004453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["S0041010104004453"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2004.10.014","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2005,3]]}}}