{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:15:49Z","timestamp":1777526149124,"version":"3.51.4"},"reference-count":40,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2005,11]]},"DOI":"10.1016\/j.toxicon.2005.07.014","type":"journal-article","created":{"date-parts":[[2005,9,16]],"date-time":"2005-09-16T16:40:03Z","timestamp":1126888803000},"page":"664-671","source":"Crossref","is-referenced-by-count":26,"title":["Molecular characterization of a neutralizing murine monoclonal antibody against Tityus serrulatus scorpion venom"],"prefix":"10.1016","volume":"46","author":[{"given":"L.M.","family":"Alvarenga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.A.","family":"Machado de Avila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.R.","family":"Amim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.S.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Kalapothakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.E.","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.G.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Granier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ch\u00e1vez-Ol\u00f3rtegui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2005.07.014_bib42","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.toxicon.2003.09.006","article-title":"Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assay","volume":"42","author":"Alvarenga","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib1","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1016\/0006-291X(84)91211-7","article-title":"Amino acid sequence of toxin VII, a \u03b2-toxin from the venom of the scorpion Tityus serrulatus","volume":"122","author":"Bechis","year":"1984","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2005.07.014_bib2","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.4049\/jimmunol.136.9.3371","article-title":"Specifity and neutralizing capacity of antibodies elicited by Synthetic peptide of scorpion toxin","volume":"136","author":"Bahraoui","year":"1986","journal-title":"J. Immunol."},{"key":"10.1016\/j.toxicon.2005.07.014_bib3","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1046\/j.1432-1327.1999.00620.x","article-title":"Mapping of an epitope recognized by a neutralizing monoclonal antibody specific to toxin Cn2 from the scorpion Centruroides noxius, using discontinuous synthetic peptides","volume":"264","author":"Calderon-Aranda","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2005.07.014_bib4","series-title":"Natural Toxins","first-page":"61","article-title":"Signs, symptoms and treatment of severe scorpion poisoning in children","author":"Campos","year":"1980"},{"key":"10.1016\/j.toxicon.2005.07.014_bib5","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/0264-410X(91)90012-U","article-title":"In vivo protection against scorpion toxins by liposomal immunization","volume":"9","author":"Ch\u00e1vez-Ol\u00f3rtegui","year":"1991","journal-title":"Vaccine"},{"key":"10.1016\/j.toxicon.2005.07.014_bib6","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/0041-0101(94)90323-9","article-title":"ELISA for the detection of toxic antigens in experimental and clinical envenoming by Tityus serrulatus scorpion venom","volume":"32","author":"Chav\u00e9z-Ol\u00f3rtegui","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib7","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0041-0101(96)00133-X","article-title":"Neutralizing capacity of antibodies elicited by a non-toxic protein purified from the venon of the escorpion Tityus serrulatus","volume":"35","author":"Ch\u00e1vez-Ol\u00f3rtegui","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib8","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/S0161-5890(01)00117-1","article-title":"Molecular basis for the cross-reactive of antibodies elicited by a natural anatoxin with \u03b1- and \u03b2-toxins from the venom of Tityus serrulatus scorpion","volume":"38","author":"Ch\u00e1vez-Ol\u00f3rtegui","year":"2002","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.toxicon.2005.07.014_bib10","doi-asserted-by":"crossref","first-page":"5045","DOI":"10.1074\/jbc.M408427200","article-title":"Common features in the functional surface of scorpion beta-toxins and elements that confer specificity for insect and mammalian voltage-gated sodium channels","volume":"280","author":"Cohen","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2005.07.014_bib11","first-page":"227","article-title":"Tityus serrulatus scorpion venom toxins display a complex pattern of antigenic reactivity","volume":"223","author":"De Lima","year":"1993","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib43","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/0041-0101(81)90044-1","article-title":"Scorpion venoms and neurotoxins: an immunological study","volume":"19","author":"Delori","year":"1981","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib12","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1111\/j.1432-1033.1996.0727r.x","article-title":"A conserved region of scorpion toxins rendered immunogenic induces broadly cross-reactive, neutralizing antibodies","volume":"242","author":"Devaux","year":"1996","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2005.07.014_bib13","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0022-1759(02)00338-1","article-title":"A strategy for inducing an immune response against Androctonus australis scorpion venom toxin I in mice. Production of high-affinity monoclonal antibodies and their use in a sensitive two-site immunometric assay","volume":"271","author":"Devaux","year":"2002","journal-title":"J Immunol. Methods"},{"key":"10.1016\/j.toxicon.2005.07.014_bib14","series-title":"Arthropod Venoms","first-page":"379","article-title":"Chemical and pharmacological aspects of Tityinae venom","author":"Diniz","year":"1978"},{"key":"10.1016\/j.toxicon.2005.07.014_bib16","doi-asserted-by":"crossref","first-page":"9217","DOI":"10.1016\/S0040-4020(01)85612-X","article-title":"Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support","volume":"48","author":"Frank","year":"1992","journal-title":"Tetrahedron"},{"key":"10.1016\/j.toxicon.2005.07.014_bib17","doi-asserted-by":"crossref","first-page":"119","DOI":"10.2174\/1386207033329779","article-title":"Potential of peptides selected from random phage-displayed libraries to mimic conformational epitopes: a study on scorpion toxin Cn2 and the neutralizing monoclonal antibody BCF2","volume":"6","author":"Gazarian","year":"2003","journal-title":"Comb. Chem. High Throughput Screen."},{"key":"10.1016\/j.toxicon.2005.07.014_bib18","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0041-0101(82)90240-9","article-title":"Epidemiological and clinical characteristics of the scorpion envenomation in Tunisia","volume":"20","author":"Goyffon","year":"1982","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib19","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1590\/S0100-879X1997000700010","article-title":"Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment","volume":"30","author":"Guatimosim-Fonseca","year":"1997","journal-title":"Brazilian J. Med Biol. Res."},{"key":"10.1016\/j.toxicon.2005.07.014_bib21","first-page":"79","article-title":"The isolation of nerve endings from brain: an electron microscope study of cell fragments derived by homogenization of centrifugation","volume":"96","author":"Gray","year":"1962","journal-title":"J. Anat."},{"key":"10.1016\/j.toxicon.2005.07.014_bib22","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1016\/S0006-291X(80)80082-9","article-title":"Two types of scorpion neurotoxins characterized by their binding to separate receptor sites on rat brain synaptossomes","volume":"95","author":"Jover","year":"1980","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2005.07.014_bib23","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1002\/bip.360221211","article-title":"Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features","volume":"22","author":"Kabsch","year":"1983","journal-title":"Biopolymers"},{"key":"10.1016\/j.toxicon.2005.07.014_bib24","series-title":"Biological Standardization","first-page":"37","author":"Karber","year":"1937"},{"key":"10.1016\/j.toxicon.2005.07.014_bib25","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/S0041-0101(96)00187-0","article-title":"Molecular characterization, antigenicity and immunogenicity of anatoxin polymeric forms conferring protection against scorpion venoms","volume":"35","author":"Kharrat","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib26","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1002\/(SICI)1097-0134(199707)28:3<360::AID-PROT6>3.0.CO;2-G","article-title":"Refined solution structure of the anti-mammal and anti-insect LqqIII scorpion toxin: comparison with other scorpion toxins","volume":"28","author":"Landon","year":"1997","journal-title":"Proteins"},{"key":"10.1016\/j.toxicon.2005.07.014_bib27","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0022-1759(02)00140-0","article-title":"Application of the Spot method to the identification of peptides and amino acids from the antibody paratope that contribute to antigen binding.","volume":"267","author":"Laune","year":"2002","journal-title":"J. Immun. Methods"},{"key":"10.1016\/j.toxicon.2005.07.014_bib28","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.toxicon.2004.04.015","article-title":"Molecular characterization of protective antibodies raised in mice by Tityus serrulatus scorpion venom toxins conjugated to bovine serum albumin","volume":"44","author":"Machado de Avila","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib29","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/nt.2620010211","article-title":"The \u03b2-type toxin TsII from the scorpion Tityus serrulatus: amino acid sequence determination and asessment of biological and antigenic properties","volume":"1","author":"Mansuelle","year":"1992","journal-title":"Natural Toxins"},{"key":"10.1016\/j.toxicon.2005.07.014_bib30","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0014-5793(94)80496-6","article-title":"Biochemical, pharmacological an genomic characterization of TsIV, and \u03b1 toxin from the venom of the South Amaricam scorpion Tityus serrulatus","volume":"342","author":"Martin-Euclaire","year":"1994","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2005.07.014_bib31","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1111\/j.1432-1033.1970.tb01111.x","article-title":"Purification of animal neurotoxins. Isolation and characterization of eleven neurotoxins from the venoms of scorpions Androctonus australis Hector, Buthus occitanus and Leiurus quinquestratus","volume":"16","author":"Miranda","year":"1970","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2005.07.014_bib32","first-page":"151","article-title":"Improved performances of spot multiple peptide synthesis","volume":"9","author":"Molina","year":"1996","journal-title":"Peptide Res."},{"key":"10.1016\/j.toxicon.2005.07.014_bib33","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0041-0101(97)00103-7","article-title":"In vivo protection against Tityus serrulatus scorpion toxins by immunization of mice with a non-toxic protein","volume":"36","author":"Moreira-Ferreira","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2005.07.014_bib34","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/jmbi.1999.2868","article-title":"Crystal structure of neurotoxin Ts1 from Tityus serrulatus provides insights into the specificity and toxicity of scorpion toxins","volume":"290","author":"Polikarpov","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2005.07.014_bib35","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/0003-9861(77)90053-4","article-title":"Purification and properties of mammalian toxins from the venom of Brazilian Scorpion Tityus serrulatus Lutz and Mello","volume":"180","author":"Possani","year":"1977","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2005.07.014_bib36","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/BF02906497","article-title":"Purification and chemical characterization of the major toxins from the venom of the Brazilian scorpion Tityus serrulatus","volume":"46","author":"Possani","year":"1981","journal-title":"Carlsberg Res. Commun."},{"key":"10.1016\/j.toxicon.2005.07.014_bib37","first-page":"750","article-title":"Scorpion toxins from Centruroires noxius and Tityus serrulatus. Primary structures and sequence comparison by metric analysis","volume":"739","author":"Possani","year":"1985","journal-title":"Biochem J."},{"key":"10.1016\/j.toxicon.2005.07.014_bib38","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1046\/j.1432-1327.1999.00625.x","article-title":"Scorpion toxins specific for Na+-channels","volume":"264","author":"Possani","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2005.07.014_bib39","series-title":"Advances in Cytopharmacology","first-page":"325","article-title":"Scorpion toxins: chemistry and mode of action","author":"Rochat","year":"1977"},{"key":"10.1016\/j.toxicon.2005.07.014_bib40","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modelling by satisfaction of spatial restraints","volume":"234","author":"Sali","year":"1993","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2005.07.014_bib41","first-page":"249","article-title":"Purification and partial characterization of a second toxin from the scorpion Tityus serrulatus","volume":"55B","author":"Toledo","year":"1976","journal-title":"Comp. Biochem. Physiol."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010105002606?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010105002606?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T15:48:43Z","timestamp":1683215323000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010105002606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["S0041010105002606"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2005.07.014","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2005,11]]}}}