{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T23:52:29Z","timestamp":1770508349467,"version":"3.49.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2007,6]]},"DOI":"10.1016\/j.toxicon.2007.02.003","type":"journal-article","created":{"date-parts":[[2007,2,23]],"date-time":"2007-02-23T16:36:49Z","timestamp":1172248609000},"page":"1099-1108","source":"Crossref","is-referenced-by-count":8,"title":["The co-purification of a lectin (BJcuL) with phospholipases A2 from Bothrops jararacussu snake venom by immunoaffinity chromatography with antibodies to crotoxin"],"prefix":"10.1016","volume":"49","author":[{"given":"Paulo C\u00e9sar","family":"Gomes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo A.","family":"Machado de \u00c1vila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wany","family":"Selena Maria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Consuelo Latorre","family":"Fortes-Dias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Ch\u00e1vez-Ol\u00f3rtegui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2007.02.003_bib1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/0167-4781(91)90132-6","article-title":"Analysis of cDNAs encoding the two subunits of crotoxin, a phospholipase A2 neurotoxin from rattlesnake venom: the acidic non enzymatic subunit derives from a phospholipase A2-like precursor","volume":"1088","author":"Bouchier","year":"1991","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2007.02.003_bib2","first-page":"265","article-title":"Contribui\u00e7\u00e3o ao estudo do veneno ophidico. III. Tratamento das mordeduras de cobras","volume":"6","author":"Brazil","year":"1903","journal-title":"Ver. Med. S\u00e3o Paulo"},{"key":"10.1016\/j.toxicon.2007.02.003_bib3","first-page":"21","article-title":"Nova contribui\u00e7\u00e3o ao estudo do veneno ophidico","volume":"19","author":"Brazil","year":"1909","journal-title":"Ver. Med. S\u00e3o Paulo"},{"key":"10.1016\/j.toxicon.2007.02.003_bib4","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/0041-0101(76)90010-6","article-title":"Enzymatic characteristics of Crotalus phospholipase A2 and the crotoxin complex","volume":"14","author":"Breithaupt","year":"1975","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib5","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/S0041-0101(96)00134-1","article-title":"Effect of Crotalus venom on the humoral and cellular immune response","volume":"35","author":"Cardoso","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib6","first-page":"933","article-title":"Isolation and characterization of a new lectin from the venom of the snake Bothrops jararacussu","volume":"44","author":"Carvalho","year":"1998","journal-title":"Biochem. Mol. Biol. Int."},{"key":"10.1016\/j.toxicon.2007.02.003_bib7","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1016\/S0041-0101(01)00106-4","article-title":"Effect of BJcuL (a lectin from the venom of the snake Bothrops jararacussu) on adhesion and growth of tumor cells and endothelial cells","volume":"39","author":"Carvalho","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib8","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1023\/A:1014131115951","article-title":"Primary structure characterization of Bothrops jararacussu snake venom lectin","volume":"21","author":"Carvalho","year":"2002","journal-title":"J. Prot. Chem."},{"key":"10.1016\/j.toxicon.2007.02.003_bib9","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/BF01024915","article-title":"Bothropstoxin-I\u2013amino acid sequence and function","volume":"12","author":"Cintra","year":"1993","journal-title":"J. Prot. Chem."},{"key":"10.1016\/j.toxicon.2007.02.003_bib10","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0041-0101(96)00119-5","article-title":"An enzyme-linked immunosorbent assay (ELISA) that discriminates between the venoms of Brazilian Bothrops species and Crotalus durissus","volume":"35","author":"Chavez-Olortegui","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib11","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1590\/S0036-46651992000200001","article-title":"The efficacy of the bothropic-crotalic antivenom in the neutralization of the main Bothrops jararacussu venom effects","volume":"34","author":"Dos-Santos","year":"1992","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"10.1016\/j.toxicon.2007.02.003_bib12","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1021\/bi00402a036","article-title":"Crotoxin, a phospholipase A2 neurotoxin from the South American rattlesnake Crotalus durissus terrificus: purification of several isoforms and comparison of their molecular structure and of their biological activity","volume":"27","author":"Faure","year":"1988","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2007.02.003_bib13","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1432-1033.1993.tb17946.x","article-title":"Comparison of crotoxin isoforms reveals that stability of the complex plays a major role in its pharmacological action","volume":"214","author":"Faure","year":"1993","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2007.02.003_bib14","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1432-1033.1994.tb18978.x","article-title":"The origin of the diversity of crotoxin isoforms in the venom of Crotalus durissus terrificus","volume":"223","author":"Faure","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2007.02.003_bib15","series-title":"Probit Analysis","author":"Finney","year":"1971"},{"key":"10.1016\/j.toxicon.2007.02.003_bib16","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1016\/0041-0101(90)90162-Z","article-title":"Protection against the lethal effects of Crotalus durissus terrificus (South American rattlesnake) venom in animals immunized with crotoxin","volume":"28","author":"Freitas","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib17","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0041-0101(88)90010-4","article-title":"An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica","volume":"26","author":"Gutierrez","year":"1988","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib18","doi-asserted-by":"crossref","first-page":"721","DOI":"10.2165\/00003088-200342080-00002","article-title":"Pharmacokinetic-pharmacodynamic relationships of immunoglobulin therapy for envenomation","volume":"42","author":"Gutierrez","year":"2003","journal-title":"Clin. Pharmacokinetics"},{"key":"10.1016\/j.toxicon.2007.02.003_bib19","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1073\/pnas.68.7.1560","article-title":"Biological roles of the two components of crotoxin","volume":"68","author":"Hendon","year":"1971","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.toxicon.2007.02.003_bib20","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s10930-005-6718-z","article-title":"Biochemical, pharmacological and structural characterization of a new PLA2 from Crotalus durissus terrificus (South American rattlesnake) venom","volume":"24","author":"Hernandez-Oliveira","year":"2005","journal-title":"Protein J."},{"key":"10.1016\/j.toxicon.2007.02.003_bib21","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/0041-0101(88)90244-9","article-title":"Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin","volume":"26","author":"Homsi-Brandeburgo","year":"1988","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib22","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.biochi.2004.02.002","article-title":"Analysis of Bothrops jararacussu venomous gland transcriptome focusing on structural and functional aspects\u2014I-gene expression profile of highly expressed phospholipases A2","volume":"86","author":"Kashima","year":"2004","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.02.003_bib23","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2007.02.003_bib24","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/j.toxicon.2003.11.008","article-title":"An overview of lysine-49 phospholipase A2 myotoxins from crotalid snakes and their structural determinants of myotoxic action","volume":"42","author":"Lomonte","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib25","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1016\/S0041-0101(98)00077-4","article-title":"Neutralizing potency of horse antibothropic antivenom. Correlation between in vivo and in vitro methods","volume":"36","author":"Maria","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib26","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1093\/qjmed\/90.5.323","article-title":"Snake bites by the jararacu\u00e7u (Bothrops jararacussu): clinicopathological studies of 29 proven cases in S\u00e3o Paulo State, Brazil","volume":"90","author":"Milani-J\u00fanior","year":"1997","journal-title":"Quart. J. Med."},{"key":"10.1016\/j.toxicon.2007.02.003_bib27","unstructured":"Minist\u00e9rio da Saude do Brasil, 1990. Secretaria Nacional de A\u00e7\u00f5es B\u00e1sicas de Sa\u00fade, Acidentes of\u00eddicos: Contribui\u00e7\u00e3o ao estudo da morbidade."},{"key":"10.1016\/j.toxicon.2007.02.003_bib28","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1023\/A:1015320616206","article-title":"Structural and functional characterization of basic PLA2 isolated from Crotalus durissus terrificus venom","volume":"21","author":"Oliveira","year":"2002","journal-title":"J. Prot. Chem."},{"key":"10.1016\/j.toxicon.2007.02.003_bib29","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/S0041-0101(01)00107-6","article-title":"Neutralization of the pharmacological effects of bothropstoxin-I from Bothrops jararacussu (jararacu\u00e7u) venom by crotoxin antiserum and heparin","volume":"39","author":"Oshima-Franco","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib30","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/j.1742-7843.2004.pto_950405.x","article-title":"The pre-synaptic activity of Bothropstoxin I, a myotoxin from Bothrops jararacussu snake venom","volume":"95","author":"Oshima-Franco","year":"2004","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"10.1016\/j.toxicon.2007.02.003_bib31","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.toxicon.2005.08.012","article-title":"Biological activities of a lectin from Bothrops jararacussu snake venom","volume":"47","author":"Panunto","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib32","first-page":"4023","article-title":"The effect of a lectin from the venom of the snake Bothrops jararacussu, on tumor cell proliferation","volume":"19","author":"Pereira-Bittencourt","year":"1999","journal-title":"Anticancer Res."},{"key":"10.1016\/j.toxicon.2007.02.003_bib33","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0041-0101(92)90505-Y","article-title":"Biological activities of venoms from South American snakes","volume":"30","author":"Sanchez","year":"1992","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib34","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0041-0101(80)90002-1","article-title":"Purification of gyroxin from a South American rattlesnake (Crotalus durissus terrificus) venom","volume":"18","author":"Seki","year":"1980","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib35","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.toxicon.2005.04.020","article-title":"Antibodies to a fragment of the Bothrops moojeni L-amino acid oxidase cross-react with snake venom components unrelated to the parent molecule","volume":"46","author":"St\u00e1beli","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib36","series-title":"Animais Pe\u00e7onhentos","author":"Soerensen","year":"1990"},{"key":"10.1016\/j.toxicon.2007.02.003_bib37","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/S0041-0101(03)00085-0","article-title":"Structural, enzymatic and biological properties of new PLA(2) isoform from Crotalus durissus terrificus venom","volume":"41","author":"Toyama","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.02.003_bib38","first-page":"981","article-title":"Pharmacology of crystalline crotoxin. II. Neuromuscular blocking action","volume":"33","author":"Vital Brazil","year":"1966","journal-title":"Mem. Inst. Butantan"},{"key":"10.1016\/j.toxicon.2007.02.003_bib39","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1006\/prep.2000.1353","article-title":"Refolding and purification of bothropstoxin I, a Lys-49-Phospholipase A2 homologue, expressed as inclusion bodies in Escherichia coli","volume":"21","author":"Ward","year":"2001","journal-title":"Protein Exp. Purif."},{"key":"10.1016\/j.toxicon.2007.02.003_bib40","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.toxicon.2004.05.029","article-title":"Comparison of the neurotoxic and myotoxic effects of Brazilian Bothrops venoms and their neutralization by commercial antivenom","volume":"44","author":"Zamuner","year":"2004","journal-title":"Toxicon"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010107000566?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010107000566?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T22:07:12Z","timestamp":1556143632000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010107000566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["S0041010107000566"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2007.02.003","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2007,6]]}}}