{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:10:16Z","timestamp":1791256216495,"version":"4.1.0"},"reference-count":47,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2007,7]]},"DOI":"10.1016\/j.toxicon.2007.03.002","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T10:55:26Z","timestamp":1173956126000},"page":"120-134","source":"Crossref","is-referenced-by-count":47,"title":["Cytotoxic, thrombolytic and edematogenic activities of leucurolysin-a, a metalloproteinase from Bothrops leucurus snake venom"],"prefix":"10.1016","volume":"50","author":[{"given":"L.H.","family":"Gremski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.M.","family":"Chaim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.S.","family":"Paludo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.B.","family":"Sade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.F.","family":"Otuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Gremski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.F.","family":"Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.S.","family":"Veiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2007.03.002_bib1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0041-0101(03)00230-7","article-title":"Pharmacological characterization of mouse hind paw oedema induced by Bothrops insularis (jararaca ilhoa) snake venom","volume":"42","author":"Barbosa","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.biochi.2005.07.008","article-title":"Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom","volume":"88","author":"Bello","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.03.002_bib3","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0163-7258(94)90049-3","article-title":"Hemorrhagic metalloproteinases from snake venoms","volume":"62","author":"Bjarnason","year":"1994","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.toxicon.2007.03.002_bib4","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/0041-0101(94)90330-1","article-title":"Effect of various Viperidae and Crotalidae snake venoms on endothelial cells in vitro","volume":"32","author":"Borkow","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib5","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1016\/0041-0101(95)00078-Z","article-title":"In vitro activity of BaH1, the main hemorrhagic toxin of Bothrops asper snake venom on bovine endothelial cells","volume":"33","author":"Borkow","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib6","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2007.03.002_bib7","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0041-0101(01)00245-8","article-title":"Pharmacological characterization and neutralization of the venoms used in the production of Bothropic antivenom in Brazil","volume":"40","author":"Camey","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib8","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.taap.2005.05.015","article-title":"Brown spider dermonecrotic toxin directly induces nephrotoxicity","volume":"211","author":"Chaim","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib9","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1006\/taap.2002.9404","article-title":"Impairment of the cell-to-matrix adhesion and cytotoxicity induced by Bothrops moojeni snake venom in cultured renal tubular epithelia","volume":"181","author":"Collares-Buzato","year":"2002","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib10","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0003-9861(03)00298-4","article-title":"BaG, a new dimeric metalloproteinase\/disintegrin from the Bothrops alternatus snake venom that interacts with \u03b15\u03b21 integrin","volume":"416","author":"Cominetti","year":"2003","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2007.03.002_bib11","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/S0041-0101(01)00275-6","article-title":"Inhibition of melanoma cells tumorigenicity by the snake venom toxin jararhagin","volume":"40","author":"Corr\u00eaa","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib12","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.biochi.2006.02.008","article-title":"Molecular cloning and functional characterization of two isoforms of dermonecrotic toxin from Loxosceles intermedia (Brown spider) venom gland","volume":"88","author":"da Silveira","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.03.002_bib13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.intimp.2005.08.002","article-title":"Differential modulation of cell recruitment and acute edema in a model of Polybia paulista venom-induced inflammation","volume":"6","author":"de Paula","year":"2006","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib14","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1002\/jcb.20322","article-title":"Characterization of events associated with apoptosis\/anoikis induced by snake venom metalloproteinase BaP1 on human endothelial cells","volume":"94","author":"D\u00edaz","year":"2005","journal-title":"J. Cell Biochem."},{"key":"10.1016\/j.toxicon.2007.03.002_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijc.2910200102","article-title":"Binding of soluble forms of fibroblast surface protein, fibronectin, to collagen","volume":"20","author":"Engvall","year":"1977","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.toxicon.2007.03.002_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2005.06.007","article-title":"Role of the snake venom toxin jararhagin in proinflammatory pathogenesis: in vitro and in vivo gene expression analysis of the effects of the toxin","volume":"441","author":"Gallagher","year":"2005","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2007.03.002_bib17","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0049-3848(97)00066-2","article-title":"Further characterization and thrombolytic activity in a rat model of a fibrinogenase from Vipera lebetina venom","volume":"86","author":"Gasmi","year":"1997","journal-title":"Thromb. Res."},{"key":"10.1016\/j.toxicon.2007.03.002_bib18","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0003-9861(91)90462-R","article-title":"Purification and characterization of a fibrinolytic enzyme from venom of the southern copperhead snake (Agkistrodon contortrix contortrix)","volume":"289","author":"Guan","year":"1991","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2007.03.002_bib19","first-page":"211","article-title":"Local tissue damage induced by Bothrops snake venoms: a review","volume":"51","author":"Guti\u00e9rrez","year":"1989","journal-title":"Mem. Inst. Butantan"},{"key":"10.1016\/j.toxicon.2007.03.002_bib20","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0300-9084(00)01163-9","article-title":"Snake venom metalloproteinases: their role in the pathogenesis of local tissue damage","volume":"82","author":"Guti\u00e9rrez","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.03.002_bib21","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0041-0101(94)00138-X","article-title":"Isolation and characterization of a metalloproteinase with weak hemorrhagic activity from the venom of the snake Bothrops asper (Terciopelo)","volume":"33","author":"Guti\u00e9rrez","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib22","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.toxicon.2005.02.029","article-title":"Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage","volume":"45","author":"Guti\u00e9rrez","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.mvr.2005.10.007","article-title":"Blood flow is required for rapid endothelial cell damage induced by a snake venom hemorrhagic metalloproteinase","volume":"71","author":"Guti\u00e9rrez","year":"2006","journal-title":"Microvasc. Res."},{"key":"10.1016\/j.toxicon.2007.03.002_bib24","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.cbi.2004.09.016","article-title":"Neo-clerodane diterpenoid a new metalloprotease snake venom inhibitor from Baccharis trimera (Asteraceae): anti-proteolytic and anti-hemorrhagic properties","volume":"150","author":"Janu\u00e1rio","year":"2004","journal-title":"Chem. Bio. Interact."},{"key":"10.1016\/j.toxicon.2007.03.002_bib25","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/S0006-2952(02)01288-1","article-title":"Biochemical and biological properties of phospholipases A2 from Bothrops atrox snake venom","volume":"64","author":"Kanashiro","year":"2002","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib26","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1016\/S0041-0101(02)00171-X","article-title":"Vascular endothelial cell injury induced by Bothrops jararaca venom non-significance of hemorrhagic metalloproteinase","volume":"40","author":"Kawano","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib27","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1152\/ajpheart.00683.2005","article-title":"Reactive oxygen species, but not Ca2+ overloading trigger pH- and mitochondrial permeability transition-dependent death of adult rat myocytes after ischemia-reperfusion","volume":"290","author":"Kim","year":"2006","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib28","first-page":"143","article-title":"Ophidic accidents by Bothrops leucurus Wagler, 1824 in Bahia, Brazil","volume":"31","author":"Lira-da-Silva","year":"1993","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib29","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/0041-0101(94)90302-6","article-title":"Activity of hemorrhagic metalloproteinase BaH-1 and myotoxin II from Bothrops asper snake venom on capillary endothelial cells in vitro","volume":"32","author":"Lomonte","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib30","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jsb.2005.09.006","article-title":"Crystal structure of a non-hemorrhagic fibrin(ogen)olytic metalloproteinase complexed with a novel natural tri-peptide inhibitor from venom of Agkistrodon acutus","volume":"152","author":"Lou","year":"2005","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib31","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.cbpa.2005.12.033","article-title":"Purification and properties of a coagulant thrombin-like enzyme from the venom of Bothrops leucurus","volume":"146","author":"Magalh\u00e3es","year":"2006","journal-title":"Comp. Biochem. Phys. A"},{"key":"10.1016\/j.toxicon.2007.03.002_bib32","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0041-0101(98)00126-3","article-title":"Snake venoms and the hemostatic system","volume":"36","author":"Markland","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib33","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/0041-0101(94)90376-X","article-title":"Pathological changes induced by BaH1, a hemorrhagic proteinase isolated from Bothrops asper (Terciopelo) snake venom, on mouse capillary blood vessels","volume":"32","author":"Moreira","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib34","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0041-0101(01)00229-X","article-title":"Toxicity of South American snake venoms measured by an in vitro cell culture assay","volume":"40","author":"Oliveira","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib35","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.reprotox.2006.03.009","article-title":"Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis","volume":"22","author":"Oliveira","year":"2006","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.toxicon.2007.03.002_bib36","series-title":"Handbook of Toxinology","first-page":"602","article-title":"Locally acting agents: myotoxins, hemorrhagic toxins and dermonecrotic factors","author":"Ownby","year":"1990"},{"key":"10.1016\/j.toxicon.2007.03.002_bib37","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/S0041-0101(09)80006-8","article-title":"Pathogenesis of hemorrhage induced by bilitoxin a hemorrhagic toxin isolated from the venom of tye common cantil (Agkistrodon bilineatus bilineatus)","volume":"28","author":"Ownby","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib38","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.toxicon.2006.02.006","article-title":"The effect of brown spider venom on endothelial cell morphology and adhesive structures","volume":"47","author":"Paludo","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib39","first-page":"45","article-title":"Cytopathologic effect of Cerastes cerastes (Egyptian sand viper) venom and isolated hemorrhagic toxin on liver and kidney: an electron microscopic study","volume":"1","author":"Rahmy","year":"1992","journal-title":"J. Nat. Toxins"},{"key":"10.1016\/j.toxicon.2007.03.002_bib40","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1590\/S0037-86821997000600006","article-title":"Acidente por serpentes do g\u00eanero Bothrops: s\u00e9rie de 3.139 casos","volume":"30","author":"Ribeiro","year":"1997","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.toxicon.2007.03.002_bib41","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0300-9084(01)01282-2","article-title":"Pathological alterations induced by neuwiedase a metalloproteinase isolated from Bothrops neuwiedi snake venom","volume":"83","author":"Rodrigues","year":"2001","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.03.002_bib42","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/S0041-0101(98)00268-2","article-title":"Characterization of the local tissue damage induced by LHF-II, a metalloproteinase with weak hemorrhagic activity isolated from Lachesis muta muta snake venom","volume":"37","author":"Rucavado","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib43","doi-asserted-by":"crossref","first-page":"4706","DOI":"10.1016\/j.biomaterials.2006.05.007","article-title":"Mitochondrial membrane potential and reactive oxygen species content of endothelial and smooth muscle cells cultured on poly(\u025b-caprolactone films","volume":"27","author":"Serrano","year":"2006","journal-title":"Biomaterials"},{"key":"10.1016\/j.toxicon.2007.03.002_bib44","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1163\/156856004773121400","article-title":"Comparison between plethysmometer and micrometer methods to measure acute paw oedema for screening anti-inflammatory activity in mice","volume":"12","author":"Sharma","year":"2004","journal-title":"Inflammopharmacology"},{"key":"10.1016\/j.toxicon.2007.03.002_bib45","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.toxicon.2005.03.008","article-title":"A novel metalloprotease from Vipera lebetina venom induces human endothelial cell apoptosis","volume":"46","author":"Trummal","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.03.002_bib46","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/270530a0","article-title":"Role of prostaglandin-mediated vasodilatation in inflammation","volume":"270","author":"Williams","year":"1977","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2007.03.002_bib47","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1042\/0264-6021:3570719","article-title":"Purification, molecular cloning and mechanism of action of graminelysin I a snake-venom-derived metalloproteinase that induces apoptosis of human endothelial cells","volume":"357","author":"Wu","year":"2001","journal-title":"Biochem. J."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010107000797?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010107000797?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T19:56:16Z","timestamp":1546977376000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010107000797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["S0041010107000797"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2007.03.002","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2007,7]]}}}