{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:17:06Z","timestamp":1790756226364,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2007,12]]},"DOI":"10.1016\/j.toxicon.2007.08.001","type":"journal-article","created":{"date-parts":[[2007,8,15]],"date-time":"2007-08-15T11:15:04Z","timestamp":1187176504000},"page":"1162-1174","source":"Crossref","is-referenced-by-count":54,"title":["Biological and structural comparison of recombinant phospholipase D toxins from Loxosceles intermedia (brown spider) venom"],"prefix":"10.1016","volume":"50","author":[{"given":"Rodrigo Ot\u00e1vio S.","family":"Ribeiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga Meiri","family":"Chaim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Bertoni","family":"da Silveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza Helena","family":"Gremski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef Bacila","family":"Sade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia Sabrina","family":"Paludo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Senff-Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"de Moura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Ch\u00e1vez-Ol\u00f3rtegui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waldemiro","family":"Gremski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena B.","family":"Nader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio Sanches","family":"Veiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2007.08.001_bib2","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/BF01886859","article-title":"Compared chemical properties of dermonecrotic and lethal toxins from spiders of the genus Loxosceles (Araneae)","volume":"15","author":"Barbaro","year":"1996","journal-title":"J. Protein Chem."},{"key":"10.1016\/j.toxicon.2007.08.001_bib3","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.toxicon.2004.11.011","article-title":"Sphingomyelinase D from venoms of Loxosceles spiders: evolutionary insights from cDNA sequences and gene structure","volume":"45","author":"Binford","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2007.08.001_bib5","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.taap.2005.05.015","article-title":"Brown spider dermonecrotic toxin directly induces nephrotoxicity","volume":"211","author":"Chaim","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.toxicon.2007.08.001_bib6","series-title":"Cellular Pathology Technique","author":"Culling","year":"1985"},{"key":"10.1016\/j.toxicon.2007.08.001_bib7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/A:1023470829671","article-title":"Purification and characterization of Loxnecrogin, a dermonecrotic toxin from Loxosceles gaucho brown spider venom","volume":"22","author":"Cunha","year":"2003","journal-title":"J. Protein Chem."},{"key":"10.1016\/j.toxicon.2007.08.001_bib8","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.toxicon.2004.07.012","article-title":"Brown spider and loxoscelism","volume":"44","author":"da Silva","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib9","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0041-0101(02)00078-8","article-title":"Identification of proteases in the extract of venom glands from brown spider","volume":"40","author":"da Silveira","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib10","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.biochi.2006.02.008","article-title":"Molecular cloning and functional characterization of two isoforms of dermonecrotic toxin from Loxosceles intermedia (Brown spider) venom gland","volume":"88","author":"da Silveira","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.08.001_bib11","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.biochi.2006.12.002","article-title":"Two novel dermonecrotic toxins LiRecDT4 and LiRecDT5 from Brown spider (Loxosceles intermedia) venom: from cloning to functional characterization","volume":"89","author":"da Silveira","year":"2007","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2007.08.001_bib12","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1042\/BJ20070363","article-title":"Identification, cloning, expression and functional characterization of an astacin-like metalloprotease toxin from Loxosceles intermedia (brown spider) venom","volume":"406","author":"da Silveira","year":"2007","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxicon.2007.08.001_bib13","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.toxicon.2004.05.028","article-title":"Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Loxosceles intermedia","volume":"44","author":"de Castro","year":"2004","journal-title":"Toxicon."},{"key":"10.1016\/j.toxicon.2007.08.001_bib14","first-page":"197","article-title":"An interactive graphic program for calculating the secondary structure content of proteins from dichroism spectrum","volume":"2","author":"Deleage","year":"1993","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.toxicon.2007.08.001_bib15","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0041-0101(97)00083-4","article-title":"Detection and characterization of metalloproteinases with gelatinolytic, fibronectinolytic and fibrinogenolytic activities in brown spider (Loxosceles intermedia) venom","volume":"36","author":"Feitosa","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib16","series-title":"Culture of Animal Cells: A Manual of Basic Techniques","author":"Freshney","year":"2000"},{"key":"10.1016\/j.toxicon.2007.08.001_bib17","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/00000441-199210000-00008","article-title":"Loxoscelism","volume":"304","author":"Futrell","year":"1992","journal-title":"Am. J. Med. Sci."},{"key":"10.1016\/j.toxicon.2007.08.001_bib18","series-title":"Antibodies: A Laboratory Manual","author":"Harlow","year":"1988"},{"key":"10.1016\/j.toxicon.2007.08.001_bib19","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.annemergmed.2004.08.028","article-title":"Loxoscelism: old obstacles, new directions","volume":"44","author":"Hogan","year":"2004","journal-title":"Ann. Emerg. Med."},{"key":"10.1016\/j.toxicon.2007.08.001_bib20","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1016\/S0041-0101(02)00201-5","article-title":"Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom","volume":"40","author":"Kalapothakis","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib21","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2007.08.001_bib22","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1042\/BJ20050043","article-title":"Brown recluse spider (Loxosceles reclusa) venom phospholipase D (PLD) generates lysophosphatidic acid (LPA)","volume":"391","author":"Lee","year":"2005","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxicon.2007.08.001_bib23","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1177\/002215540405200404","article-title":"Experimental evidence for a direct cytotoxicity of Loxosceles intermedia (brown spider) venom on renal tissue","volume":"52","author":"Luciano","year":"2004","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.toxicon.2007.08.001_bib24","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1002\/pmic.200401096","article-title":"Proteome analysis of brown spider venom: identification of loxnecrogin isoforms in Loxosceles gaucho venom","volume":"5","author":"Machado","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.toxicon.2007.08.001_bib25","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1002\/bies.20081","article-title":"The ins and outs of lysophosphatidic acid signaling","volume":"26","author":"Moolenaar","year":"2004","journal-title":"BioEssays"},{"key":"10.1016\/j.toxicon.2007.08.001_bib26","doi-asserted-by":"crossref","first-page":"13658","DOI":"10.1074\/jbc.M412437200","article-title":"Structural basis for metal ion coordination and the catalytic mechanism of sphingomyelinases D","volume":"8","author":"Murakami","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2007.08.001_bib27","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.bbrc.2006.01.123","article-title":"Structural insights into the catalytic mechanism of sphingomyelinases D and evolutionary relationship to glycerophosphodiester phosphodiesterases","volume":"342","author":"Murakami","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2007.08.001_bib28","unstructured":"Oliveira-Lima, A., Dias-da-Silva, W., 1970. Imunologia, Imunopatologia, Alergia\u2014M\u00e9todos, Cap\u00edtulo 6\u2014Provas Sorol\u00f3gicas com Complemento. 1a. ed., Guanabara Koogan, Rio de Janeiro, Brasil."},{"key":"10.1016\/j.toxicon.2007.08.001_bib29","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.toxicon.2006.02.006","article-title":"The effect of brown spider venom on endothelial cell morphology and adhesive structures","volume":"47","author":"Paludo","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib30","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1172\/JCI117379","article-title":"The necrotic venom of brown recluse induces dysregulated endothelial cell-dependent neutrophil activation","volume":"94","author":"Patel","year":"1994","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.toxicon.2007.08.001_bib31","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.toxicon.2004.06.013","article-title":"Genetic and enzymatic characterization of sphingomyelinase D isoforms from the North American fiddleback spiders Loxosceles boneti and Loxosceles reclusa","volume":"44","author":"Ramos-Cerrillo","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib32","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/S0041-0101(98)00268-2","article-title":"Characterization of the local tissue damage induced by LHF-II, a metalloproteinase with weak hemorrhagic activity isolated from Lachesis muta muta snake venom","volume":"37","author":"Rucavado","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2007.08.001_bib33","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1034\/j.1600-0625.2001.100602.x","article-title":"Different pathways leading to cutaneous leukocytoclastic vasculitis in mice","volume":"10","author":"Sunderk\u00f6tter","year":"2001","journal-title":"Exp. Dermatol."},{"key":"10.1016\/j.toxicon.2007.08.001_bib34","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.clindermatol.2005.11.006","article-title":"Loxoscelism","volume":"24","author":"Swanson","year":"2006","journal-title":"Clin. Dermatol."},{"key":"10.1016\/j.toxicon.2007.08.001_bib35","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.toxicon.2007.08.001_bib36","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0049-3848(01)00254-7","article-title":"In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells","volume":"102","author":"Veiga","year":"2001","journal-title":"Thromb. Res."},{"key":"10.1016\/j.toxicon.2007.08.001_bib37","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1097\/00001721-200203000-00009","article-title":"Morphological and biochemical evidence of blood vessel damage and fibrinogenolysis triggered by brown spider venom","volume":"13","author":"Zanetti","year":"2002","journal-title":"Blood Coag. Fibrinol."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010107002930?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010107002930?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,1,5]],"date-time":"2019-01-05T04:51:25Z","timestamp":1546663885000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010107002930"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":36,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["S0041010107002930"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2007.08.001","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2007,12]]}}}