{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T23:04:42Z","timestamp":1772838282448,"version":"3.50.1"},"reference-count":46,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2009,1]]},"DOI":"10.1016\/j.toxicon.2008.10.007","type":"journal-article","created":{"date-parts":[[2008,10,18]],"date-time":"2008-10-18T08:27:59Z","timestamp":1224318479000},"page":"24-32","source":"Crossref","is-referenced-by-count":38,"title":["BthMP: a new weakly hemorrhagic metalloproteinase from Bothrops moojeni snake venom"],"prefix":"10.1016","volume":"53","author":[{"given":"M\u00e1rio S\u00e9rgio R.","family":"Gomes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirian M.","family":"Mendes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bio","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo M.","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina P.","family":"Bernardes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Am\u00e9lia","family":"Hamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia M.","family":"de Alc\u00e2ntara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreimar M.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veridiana M.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria I.","family":"Homsi-Brandeburgo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2008.10.007_bib1","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.toxicon.2007.11.002","article-title":"Potency evaluation of antivenoms in Brazil: the national control laboratory experience between 2000 and 2006","volume":"51","author":"Araujo","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib2","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0041-0101(85)90374-5","article-title":"Isolation of the major proteolytic enzyme from the venom of the snake Bothrops moojeni (Caissaca)","volume":"23","author":"Assakura","year":"1985","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib3","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0041-0101(02)00279-9","article-title":"Molecular cloning and expression of structural domains of bothropasin, a P-III metalloproteinase from the venom of Bothrops jararaca","volume":"41","author":"Assakura","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib4","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.toxicon.2007.08.005","article-title":"BnP1, a novel P-I metalloproteinase from Bothrops neuwiedi venom: biological effects benchmarking relatively to jararhagin, a P-III SVMP","volume":"51","author":"Baldo","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib5","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.biochi.2005.07.008","article-title":"Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom","volume":"88","author":"Bello","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2008.10.007_bib6","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.toxicon.2007.11.017","article-title":"Isolation and\u00a0structural characterization of a new fibrin(ogen)olytic metalloproteinase from Bothrops moojeni snake venom","volume":"51","author":"Bernardes","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0163-7258(94)90049-3","article-title":"Snake venom metalloproteinases: reprolysins","volume":"62","author":"Bjarnason","year":"1994","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.toxicon.2008.10.007_bib9","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0041-0101(01)00245-8","article-title":"Pharmacologial characterization and neutralization of the venoms used in the production of bothropic antivenom in Brazil","volume":"40","author":"Camey","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib10","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.toxicon.2006.02.001","article-title":"Importance of jararhagin disintegrin-like and cysteine-rich domains in the early events of local inflammatory response","volume":"47","author":"Clissa","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib11","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/j.toxicon.2005.02.012","article-title":"Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases","volume":"45","author":"Fox","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0041-0101(99)00127-0","article-title":"Purification and characterization of BaH4, a hemorrhagic metalloproteinase from the venom of the snake Bothrops asper","volume":"38","author":"Franceschi","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib13","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.toxicon.2006.08.014","article-title":"Toxin jararhagin in low doses induces interstitial edema and increases the metabolic rate blood cells in mice","volume":"48","author":"Francisco","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib14","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.toxicon.2005.06.019","article-title":"Proteolytic, edematogenic and myotoxic activities of a hemorrhagic metalloproteinase isolated from Bothrops alternatus venom","volume":"46","author":"Gay","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib15","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0041-0101(89)90096-2","article-title":"Comparative study on coagulant, defibrinating, fibrinolytic and fibrinogenolytic activities of Costa Rican crotaline snake venoms and their neutralization by a polyvalent antivenom","volume":"27","author":"Gene","year":"1989","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib17","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0041-0101(94)00138-X","article-title":"Isolation and characterization of a metalloproteinase with weak hemorrhagic activity from the venom of the snake Bothrops asper (Terciopelo)","volume":"33","author":"Guti\u00e9rrez","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib18","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0300-9084(00)01163-9","article-title":"Snake venom metalloproteinases: their role in the pathogenesis of local tissue damage","volume":"82","author":"Guti\u00e9rrez","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2008.10.007_bib19","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.toxicon.2005.02.029","article-title":"Hemorrhagic induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage","volume":"45","author":"Guti\u00e9rrez","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib20","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.mvr.2005.10.007","article-title":"Blood flow is required for rapid endothelial cell damage induced by a snake venom hemorrhagic metalloprotease","volume":"71","author":"Guti\u00e9rrez","year":"2006","journal-title":"Microvasc. Res."},{"key":"10.1016\/j.toxicon.2008.10.007_bib21","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.toxicon.2007.03.002","article-title":"Cytotoxic, thrombolytic and edematogenic activities of leucurolysin-a, a metalloproteinase from Bothrops leucurus snake venom","volume":"50","author":"Gremski","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib22","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/0003-2697(64)90200-3","article-title":"A micro-biuret method for estimating proteins","volume":"9","author":"Itzhaki","year":"1964","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2008.10.007_bib23","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1016\/S0041-0101(96)00108-0","article-title":"Snake venom metalloproteinases: structure, function and relationship to the ADAMS family of proteins","volume":"34","author":"Jia","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib24","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2008.10.007_bib25","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.toxicon.2005.02.013","article-title":"Jararhagin and its multiple effects on hemostasis","volume":"45","author":"Laing","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib26","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.cbpa.2005.12.033","article-title":"Purification and properties of a coagulant thrombin-like enzyme from the venom of Bothrops leucurus","volume":"146","author":"Magalh\u00e3es","year":"2007","journal-title":"Comp. Biochem. Physiol. A"},{"key":"10.1016\/j.toxicon.2008.10.007_bib27","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.1016\/j.peptides.2007.10.010","article-title":"Molecular and functional characterization of a new non-hemorrhagic metalloprotease from Bothrops jararacussu snake venom with antiplatelet activity","volume":"28","author":"Marcussi","year":"2007","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2008.10.007_bib28","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1016\/S0041-0101(02)00116-2","article-title":"N-terminal amino acid sequences and some characteristics of fibrinolytic\/hemorrhagic metalloproteinases purified from Bothrops jararaca venom","volume":"40","author":"Maruyama","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib29","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0041-0101(98)00126-3","article-title":"Snake venoms and the hemostatic system","volume":"36","author":"Markland","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib30","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.toxicon.2004.06.002","article-title":"A new hemorrhagic metalloprotease from Bothrops jararacussu snake venom: isolation and biochemical characterization","volume":"44","author":"Mazzi","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jmgm.2006.09.010","article-title":"Molecular characterization and phylogenetic analysis of BjussuMP-I: a RGD-P-III class hemorrhagic metalloprotease from Bothrops jararacussu snake venom","volume":"26","author":"Mazzi","year":"2007","journal-title":"J. Mol. Graph. Model."},{"key":"10.1016\/j.toxicon.2008.10.007_bib8","series-title":"Manual de Diagnostico e Tratamento de Acidentes por Animais Pe\u00e7onhentos","author":"Minist\u00e9rio da Sa\u00fade, Minist\u00e9rio Nacional de Sa\u00fade","year":"2001"},{"key":"10.1016\/j.toxicon.2008.10.007_bib32","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.toxicon.2006.07.028","article-title":"Effect of rACLF, a recombinant snake venom metallopeptidase on cell viability, chemokine expression and degradation of extracellular matrix proteins","volume":"48","author":"Moraes","year":"2006","journal-title":"Toxicon"},{"issue":"3","key":"10.1016\/j.toxicon.2008.10.007_bib33","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.biochi.2007.11.009","article-title":"Collagen binding is a key factor for the hemorrhagic activity of snake venom metalloproteinases","volume":"90","author":"Moura-da-Silva","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2008.10.007_bib34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/0003-9861(84)90393-X","article-title":"Isolation and biochemical characterization of hemorrhagic toxin from the venom of Crotalus atrox (western diamondback rattlesnake)","volume":"321","author":"Nikai","year":"1984","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2008.10.007_bib35","first-page":"328","article-title":"Review: snake venom metalloproteases \u2013 structure and function of catalytic and disintegrin domains","volume":"142","author":"Ramos","year":"2006","journal-title":"Comp. Biochem. Physiol. C"},{"key":"10.1016\/j.toxicon.2008.10.007_bib36","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/195281a0","article-title":"Disk electrophoresis of basic proteins and peptides on polyacrylamide gels","volume":"4838","author":"Reisfeld","year":"1962","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2008.10.007_bib37","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1006\/abbi.2000.1958","article-title":"Structural and functional characterization of neuwiedase, a nonhemorrhagic fibrin(ogen)olytic metalloprotease from Bothrops neuwiedi snake venom","volume":"381","author":"Rodrigues","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2008.10.007_bib38","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0300-9084(01)01282-2","article-title":"Pathological alterations induced by neuwidase, a metalloproteinase isolated from Bothrops neuwiedi snake venom","volume":"83","author":"Rodrigues","year":"2001","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2008.10.007_bib39","first-page":"193","article-title":"Structural and functional characterization of a P-III metalloproteinase, leucurolysin-B, from Bothrops leucurus venom","volume":"468","author":"Sanchez","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2008.10.007_bib40","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.bbapap.2006.03.004","article-title":"Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiara","volume":"1764","author":"Senis","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2008.10.007_bib41","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/0041-0101(93)90182-I","article-title":"Basic proteinases from Bothrops moojeni (cai\u00e7aca) venom-I. Isolation and activity of two serine proteinases MSPI and MSPII on synthetic substrates and platelet aggregation","volume":"31","author":"Serrano","year":"1993","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib42","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/0041-0101(93)90183-J","article-title":"Basic proteinases from Bothrops moojeni (cai\u00e7aca) venom-II. Isolation of the metalloproteinase MPB. Comparison of the proteolytic activity on natural substrates by MPB, MSPI and MSPII","volume":"31","author":"Serrano","year":"1993","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib45","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.toxicon.2004.11.010","article-title":"Purification and characterization of a hemorrhagic metalloproteinase from Bothrops lanceolatus (Fer-de-lance) snake venom","volume":"45","author":"Stroka","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib43","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0041-0101(02)00287-8","article-title":"Epidemiology of snakebite in a central region of Brasil","volume":"41","author":"Silva","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib46","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.toxicon.2005.02.027","article-title":"Snake venom fibrin(ogen)olytic enzymes","volume":"45","author":"Swenson","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2008.10.007_bib47","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0005-2795(72)90248-6","article-title":"Isoelectric focusing of proteins in polyacrylamide gels","volume":"257","author":"Vesterberg","year":"1972","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2008.10.007_bib48","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/j.biochi.2005.06.002","article-title":"Primary structure and antiplatelet mechanism of a snake venom metalloproteinase, acurhagin, from Agkistrodon acutus venom","volume":"87","author":"Wang","year":"2005","journal-title":"Biochimie"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010108005473?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010108005473?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,25]],"date-time":"2018-12-25T02:24:29Z","timestamp":1545704669000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010108005473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["S0041010108005473"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2008.10.007","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2009,1]]}}}