{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T07:33:56Z","timestamp":1772264036207,"version":"3.50.1"},"reference-count":23,"publisher":"Elsevier BV","issue":"7-8","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2009,6]]},"DOI":"10.1016\/j.toxicon.2009.02.011","type":"journal-article","created":{"date-parts":[[2009,2,22]],"date-time":"2009-02-22T10:33:10Z","timestamp":1235298790000},"page":"770-778","source":"Crossref","is-referenced-by-count":16,"title":["Heterologous expression of a gene that codes for Pg8, a scorpion toxin of Parabuthus granulatus, capable of generating protecting antibodies in mice"],"prefix":"10.1016","volume":"53","author":[{"given":"Blanca I.","family":"Garc\u00eda-G\u00f3mez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timoteo C.","family":"Olamendi-Portugal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Paniagua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jurg","family":"van der Walt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Dyason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2009.02.011_bib1","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/S0041-0101(96)00041-4","article-title":"Clinical description of Parabuthus transvaalicus scorpionism in Zimbabwe","volume":"35","author":"Bergman","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.02.011_bib2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1111\/j.1432-1033.1996.0653w.x","article-title":"A recombinant insect specific alpha-toxin of Buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins","volume":"238","author":"Bouhaouala-Zahar","year":"1996","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2009.02.011_bib3","series-title":"Envenomings and their Treatments","first-page":"311","article-title":"Scorpion stings and their treatment in M\u00e9xico","author":"Calder\u00f3n-Aranda","year":"1996"},{"key":"10.1016\/j.toxicon.2009.02.011_bib4","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1038\/3669","article-title":"Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin","volume":"1","author":"Chuang","year":"1998","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.toxicon.2009.02.011_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2007.08.018","article-title":"Southern African scorpion toxins: an overview","volume":"51","author":"Du Plessis","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.02.011_bib6","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1002\/rcm.637","article-title":"Determination of species-specific components in the venom of Parabuthus scorpions from southern Africa using matrix-assisted laser desorption time-of-flight mass spectrometry","volume":"16","author":"Dyason","year":"2002","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.toxicon.2009.02.011_bib7","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.bbagen.2007.04.006","article-title":"Four disulfide-bridged scorpion neurotoxin: heterologous expression and proper folding in vitro","volume":"1770","author":"Estrada","year":"2007","journal-title":"Biophysica Biochemica Acta"},{"key":"10.1016\/j.toxicon.2009.02.011_bib8","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0041-0101(02)00337-9","article-title":"Analysis of the immune response induced by a scorpion venom sub-fraction, a pure peptide and a recombinant peptide, against toxin Cn2 of Centruroides noxius Hoffmann","volume":"41","author":"Garcia","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.02.011_bib9","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0041-0101(99)00138-5","article-title":"Induction of neutralizing antibodies against Tityus serrulatus toxins by immunization with a recombinant non toxic protein","volume":"38","author":"Guatimosim","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.02.011_bib10","doi-asserted-by":"crossref","first-page":"2781","DOI":"10.1074\/jbc.M311029200","article-title":"A subfamily of acidic alpha-K(+) toxins","volume":"279","author":"Huys","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2009.02.011_bib11","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.toxicon.2005.08.018","article-title":"The neutralizing effect of a polyclonal antibody raised against the N-terminal eighteen-aminoacid residues of birtoxin towards the whole venom of Parabuthus transvaalicus","volume":"47","author":"Inceoglu","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.02.011_bib12","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1016\/S0264-410X(01)00379-6","article-title":"Use of fusion protein constructs to generate potent immunotherapy and protection against scorpion toxins","volume":"20","author":"Legros","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.toxicon.2009.02.011_bib13","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/S0006-291X(02)02859-0","article-title":"Scorpion toxins that block T-type Ca2+ channels in spermatogenic cells inhibit the sperm acrosome reaction","volume":"300","author":"L\u00f3pez-Gonz\u00e1lez","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2009.02.011_bib14","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1128\/MMBR.60.3.512-538.1996","article-title":"Strategies for achieving high-level expression of genes in Escherichia coli","volume":"60","author":"Makrides","year":"1996","journal-title":"Microbiological Reviews"},{"key":"10.1016\/j.toxicon.2009.02.011_bib15","first-page":"405","article-title":"Scorpionism in South Africa: a report of 42 serious scorpion envenomations","volume":"83","author":"M\u00fcller","year":"1993","journal-title":"South Afr. Med. J."},{"key":"10.1016\/j.toxicon.2009.02.011_bib16","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/S0006-291X(02)02706-7","article-title":"Two new scorpion toxins that target voltage-gated Ca2+ and Na+ channels","volume":"299","author":"Olamendi-Portugal","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2009.02.011_bib17","first-page":"339","article-title":"Scorpion venom peptides","volume":"vol. 51","author":"Possani","year":"2006"},{"key":"10.1016\/j.toxicon.2009.02.011_bib18","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1636\/H03-17","article-title":"The systematics of southern African Parabuthus Pocock (scorpiones, buthidae): revisions to the taxonomy and key to the species","volume":"32","author":"Prendini","year":"2002","journal-title":"The Journal of Arachnology"},{"key":"10.1016\/j.toxicon.2009.02.011_bib19","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1093\/protein\/5.8.827","article-title":"Recursive PCR: a novel technique for total gene synthesis","volume":"5","author":"Prodromou","year":"1992","journal-title":"Protein Engineering Design and Selection"},{"key":"10.1016\/j.toxicon.2009.02.011_bib20","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0041-0101(00)00122-7","article-title":"The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms","volume":"39","author":"Saravia","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.02.011_bib21","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","article-title":"Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa","volume":"166","author":"Sch\u00e4gger","year":"1987","journal-title":"Analytical Biochemistry"},{"key":"10.1016\/j.toxicon.2009.02.011_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2859-4-1","article-title":"Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli","volume":"4","author":"S\u00f8rensen","year":"2005","journal-title":"Microbial Cell Factories"},{"key":"10.1016\/j.toxicon.2009.02.011_bib23","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/prep.1997.0724","article-title":"In vitro folding and functional analysis of an anti-insect selective scorpion depressant neurotoxin produced in Escherichia coli","volume":"10","author":"Turkov","year":"1997","journal-title":"Protein Expression and Purification"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010109000890?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010109000890?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T01:34:49Z","timestamp":1589592889000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010109000890"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":23,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["S0041010109000890"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2009.02.011","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2009,6]]}}}