{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:21:09Z","timestamp":1777501269570,"version":"3.51.4"},"reference-count":47,"publisher":"Elsevier BV","issue":"2-3","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2010,2]]},"DOI":"10.1016\/j.toxicon.2009.09.016","type":"journal-article","created":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T09:36:55Z","timestamp":1254389815000},"page":"462-469","source":"Crossref","is-referenced-by-count":11,"title":["Molecular cloning and characterization of cDNAs encoding metalloproteinases from snake venom glands"],"prefix":"10.1016","volume":"55","author":[{"given":"Ying","family":"Jia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2009.09.016_bib1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0041-0101(02)00279-9","article-title":"Molecular cloning and expression of structural domains of bothropasin, a P-III metalloproteinase from the venom of Bothrops jararaca","volume":"41","author":"Assakura","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.toxicon.2008.05.023","article-title":"Immunization with cDNA of a novel P-II type metalloproteinase from the rattlesnake Crotalus durissus elicits antibodies which neutralize 69% of the hemorrhage induced by the whole venom","volume":"52","author":"Azofeifa-Cordero","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib3","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0934-8832(11)80175-7","article-title":"Identification of the cleavage sites by a hemorrhagic metalloproteinase in Type IV collagen","volume":"10","author":"Baramova","year":"1990","journal-title":"Matrix"},{"key":"10.1016\/j.toxicon.2009.09.016_bib4","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.biochi.2005.07.008","article-title":"Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom","volume":"88","author":"Bello","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2009.09.016_bib5","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.toxicon.2007.11.017","article-title":"Isolation and structural characterization of a new fibrinogenolytic metalloproteinase from Bothrops moojeni snake venom","volume":"51","author":"Bernardes","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib6","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0163-7258(94)90049-3","article-title":"Hemorrhagic metalloproteinases from snake venom","volume":"62","author":"Bjarnason","year":"1994","journal-title":"Pharmacol. Ther"},{"key":"10.1016\/j.toxicon.2009.09.016_bib7","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.toxicon.2005.02.024","article-title":"Snake venom disintegrins: evolution of structure and function","volume":"45","author":"Calvete","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib8","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1016\/j.biochi.2008.05.012","article-title":"P-III hemorrhagic metalloproteinases from Russell's viper venom: cloning, characterization, phylogenetic and functional site analysis","volume":"90","author":"Chen","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2009.09.016_bib9","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.toxicon.2006.07.010","article-title":"Molecular diversity of disintegrin-like domains within metalloproteinase precursors of Bothrops jararaca","volume":"48","author":"Cidade","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib10","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.bbrc.2005.01.148","article-title":"Molecular cloning, functional expression, and molecular modeling of bothrostatin, a new highly active disintegrin from Bothrops jararaca venom","volume":"329","author":"Fernandez","year":"2005","journal-title":"Biochem. Biophy. Res. Commun"},{"key":"10.1016\/j.toxicon.2009.09.016_bib11","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/j.toxicon.2005.02.012","article-title":"Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases","volume":"45","author":"Fox","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib12","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.toxicon.2005.06.019","article-title":"Proteolytic, edematogenic and myotoxic activities of a hemorrhagic metalloproteinase isolated from Bothrops alternates venom","volume":"46","author":"Gay","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/abbi.1994.1026","article-title":"cDNA sequences for four snake venom metalloproteinases: structure, classification, and their relationship to mammalian reproductive proteins","volume":"308","author":"Hite","year":"1994","journal-title":"Arch. Biochem. Biophys"},{"key":"10.1016\/j.toxicon.2009.09.016_bib14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0041-0101(03)00096-5","article-title":"The purification and partial characterization of two novel metalloproteinases from the venom of the West African carpet viper, Echis ocellatus","volume":"42","author":"Howes","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib47","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1046\/j.1432-1327.1999.00525.x","article-title":"Molecular cloning and functional characterization of a snake venom metalloproteinase","volume":"263","author":"Jeon","year":"1999","journal-title":"Eur. J. Biochem"},{"key":"10.1016\/j.toxicon.2009.09.016_bib15","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.toxicon.2008.03.028","article-title":"Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma","volume":"51","author":"Jia","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib16","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.toxicon.2009.04.008","article-title":"cDNA cloning, expression and fibrin(ogen)olytic activity of two low-molecular weight snake venom metalloproteinases","volume":"54","author":"Jia","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib17","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0020-711X(93)90016-8","article-title":"Isolation of a hemorrhagic toxin from the venom of Agkistrodon contortrix laticinctus (broad-banded copperhead) and pathogenesis of the hemorrhage induced by the toxin in mice","volume":"25","author":"Johnson","year":"1993","journal-title":"Int. J. Biochem"},{"key":"10.1016\/j.toxicon.2009.09.016_bib18","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/0041-0101(95)99241-T","article-title":"Cleavage of von Willebrand factor by jararhagin, a haemorrhagic metalloproteinase from Bothrops jaruraca venom. (Abstr)","volume":"33","author":"Kamiguti","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib19","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/0041-0101(96)00017-7","article-title":"Insights into mechanism of haemorrhage caused by snake venom metalloproteinases","volume":"34","author":"Kamiguti","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib20","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1590\/S0100-879X1998000700001","article-title":"Snake venom metalloproteinases and disintegrins: interactions with cells, Braz","volume":"31","author":"Kamiguti","year":"1998","journal-title":"J. Med. Biol. Res."},{"key":"10.1016\/j.toxicon.2009.09.016_bib21","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0041-0101(92)90869-7","article-title":"Structural domains in venom proteins: evidence that metalloproteinases and nonenzymatic platelet aggregation inhibitors (disintegrins) from snake venoms are derived by proteolysis from a common precursor","volume":"30","author":"Kini","year":"1992","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib22","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/S0041-0101(02)00081-8","article-title":"Molecular cloning and sequence analysis of cDNA encoding flavoridin, a diintegrin from the venom of Trimeresurus flavoviridis","volume":"40","author":"Kishimoto","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib23","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1016\/S0041-0101(02)00179-4","article-title":"Molecular cloning of HR1a and HR1b, high molecular hemorrhagic factors, from Trimeresurus flavoviridis venom","volume":"40","author":"Kishimoto","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib24","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.1016\/j.peptides.2007.10.010","article-title":"Molecular and functional characterization of a new non-hemorrhagic metalloproteinase from Bothrops jararacussu snake venom with antiplatelet activity","volume":"28","author":"Marcussi","year":"2007","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2009.09.016_bib25","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0041-0101(98)00126-3","article-title":"Snake venoms and their hemostatic system","volume":"36","author":"Markland","year":"1998","journal-title":"Toxicon"},{"issue":"11","key":"10.1016\/j.toxicon.2009.09.016_bib26","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1046\/j.1432-1327.2001.02246.x","article-title":"Purification, cDNA cloning and characterization of the vascular apoptosis-inducing protein, HV1, from Trimeresurus flavoviridis","volume":"268","author":"Masuda","year":"2001","journal-title":"Eur. J. Biochem"},{"key":"10.1016\/j.toxicon.2009.09.016_bib27","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/S0167-4838(99)00268-X","article-title":"Snake venom proteases affecting hemostasis and thrombosis","volume":"1477","author":"Matsui","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2009.09.016_bib28","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.toxicon.2004.06.002","article-title":"A new hemorrhagic metalloprotease from Bothrops jararacussu snake venom: isolation and biochemical characterization","volume":"44","author":"Mazzi","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib29","doi-asserted-by":"crossref","first-page":"5602","DOI":"10.1073\/pnas.92.12.5605","article-title":"Accelerated evolution in the protein-coding regions is universal in crotalinase snake venom gland phospholipase A2 isozyme genes","volume":"92","author":"Nakashima","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.toxicon.2009.09.016_bib30","doi-asserted-by":"crossref","first-page":"14248","DOI":"10.1021\/bi025876s","article-title":"A new gene structure of disintegrin family: a subunit of dimeric disintegrin have short coding region","volume":"41","author":"Okuda","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2009.09.016_bib31","first-page":"1033","article-title":"Simplified procedures for the isolation of HF3, bothropasin, disintegrin-like\/cysteine-rich protein and a novel P-I metalloproteinase from Bothrops jararaca venom","volume":"40","author":"Oliveira","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib32","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/1471-2199-8-115","article-title":"The venom gland transcriptome of the Desert Massasauga rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea)","volume":"8","author":"Pahari","year":"2007","journal-title":"BMC Mol. Biol."},{"key":"10.1016\/j.toxicon.2009.09.016_bib33","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/j.1432-1033.1994.00483.x","article-title":"Cloning of metalloprotease genes in the carpet viper (Echis pyramidum leakeyi). Further members of the metalloproteinase\/disintegrin gene family","volume":"224","author":"Paine","year":"1994","journal-title":"Eur. J. Biochem"},{"key":"10.1016\/j.toxicon.2009.09.016_bib34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1006\/bbrc.2000.3419","article-title":"Edema induction by the disintegrin-like\/cysteine-rich domains from a Bothrops atrox hemorrhagin","volume":"276","author":"Petretski","year":"2000","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"10.1016\/j.toxicon.2009.09.016_bib35","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.toxicon.2009.09.016_bib36","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S0041-0101(02)00330-6","article-title":"The efficacy of two antivenoms against the venom of North American snakes","volume":"41","author":"S\u00e1nchez","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib37","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0041-0101(02)00293-3","article-title":"Cross reactivity of three antivenoms against North American snake venoms","volume":"41","author":"S\u00e1nchez","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib38","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.abb.2007.10.002","article-title":"Structural and functional characterization of a P-III metalloproteinase, leucurolysin-B, from Bothrops leucurus venom","volume":"468","author":"Sanchez","year":"2007","journal-title":"Arch. Biochem. Biophys"},{"key":"10.1016\/j.toxicon.2009.09.016_bib39","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1006\/abbi.1995.1352","article-title":"Molecular cloning and sequence analysis of cDNAs for metalloproteinases from broad-banded copperhead Agkistrodon contortrix laticinctus","volume":"320","author":"Selistre de Araujo","year":"1995","journal-title":"Arch. Biochem. Biophys"},{"key":"10.1016\/j.toxicon.2009.09.016_bib40","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0167-4838(97)00111-8","article-title":"Analysis of a cDNA sequence encoding a novel member of the snake venommetalloproteinase, disintegrin-like, cysteine-rich (MDC) protein family from Agkistrodon contortrix laticinctus","volume":"1342","author":"Selistre de Araujo","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2009.09.016_bib41","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1042\/bj20020449","article-title":"A prothrombin activator from Bothrops erythromelas (jararaca-da-seca) snake venom: characterization and molecular cloning","volume":"369","author":"Silva","year":"2003","journal-title":"Biochem. J"},{"key":"10.1016\/j.toxicon.2009.09.016_bib42","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/j.toxicon.2007.08.002","article-title":"Molecular cloning of albolatin, a novel snake venom metalloprotease from green pit viper (Trimeresurus albolabris), and expression of its disintegrin domain","volume":"50","author":"Singhamatr","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib43","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.toxicon.2009.09.016_bib44","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.toxicon.2005.03.008","article-title":"A novel metalloprotease from Vipera lebetina venom induces human endothelial cell apoptosis","volume":"46","author":"Trummal","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib45","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.toxicon.2006.01.003","article-title":"Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland","volume":"47","author":"Wan","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2009.09.016_bib46","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1042\/bj3070411","article-title":"Molecular cloning and expression of catrocollastatin, a snake-venom protein from Crotalus atrox (western diamondback rattlesnake) which inhibits platelet adhesion to collagen","volume":"307","author":"Zhou","year":"1995","journal-title":"Biochem. J."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004101010900467X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004101010900467X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T03:23:25Z","timestamp":1544930605000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S004101010900467X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":47,"journal-issue":{"issue":"2-3","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["S004101010900467X"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2009.09.016","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2]]}}}