{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T16:32:13Z","timestamp":1793809933920,"version":"4.6.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2011,9,6]],"date-time":"2011-09-06T00:00:00Z","timestamp":1315267200000},"content-version":"vor","delay-in-days":279,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2010,12]]},"DOI":"10.1016\/j.toxicon.2010.08.008","type":"journal-article","created":{"date-parts":[[2010,9,7]],"date-time":"2010-09-07T04:31:30Z","timestamp":1283833890000},"page":"1426-1435","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1"],"prefix":"10.1016","volume":"56","author":[{"given":"Camila","family":"Dias-Lopes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liza","family":"Felicori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eneas R.M.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Roman-Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Damasceno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilia","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evanguedes","family":"Kalapothakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvair P.","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Granier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jader S.","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Guatimosim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Ch\u00e1vez-Ol\u00f3rtegui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2010.08.008_bib1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.toxicon.2003.09.006","article-title":"Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assay","volume":"42","author":"Alvarenga","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib3","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1161\/01.CIR.92.7.1927","article-title":"Serum cardiac troponin T and creatine kinase-MB elevations in murine autoimmune myocarditis","volume":"92","author":"Bachmaier","year":"1995","journal-title":"Circulation"},{"key":"10.1016\/j.toxicon.2010.08.008_bib4","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0041-0101(94)90027-2","article-title":"Antigenic cross-reactivity of venoms from medically important Loxosceles (Araneae) species in Brazil","volume":"32","author":"Barbaro","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib5","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1196\/annals.1341.008","article-title":"Calcium signaling in cardiac ventricular myocytes","volume":"1047","author":"Bers","year":"2005","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.toxicon.2010.08.008_bib6","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1161\/01.HYP.0000175813.04375.8a","article-title":"Evidence for a functional interaction of the angiotensin-(1\u20137) receptor Mas with AT1 and AT2 receptors in the mouse heart","volume":"46","author":"Castro","year":"2005","journal-title":"Hypertension"},{"key":"10.1016\/j.toxicon.2010.08.008_bib7","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.lfs.2006.09.007","article-title":"Effects of genetic deletion of angiotensin-(1\u20137) receptor Mas on cardiac function during ischemia\/reperfusion in the isolated perfused mouse heart","volume":"80","author":"Castro","year":"2006","journal-title":"Life Sci."},{"key":"10.1016\/j.toxicon.2010.08.008_bib8","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.taap.2005.05.015","article-title":"Brown spider dermonecrotic toxin directly induces nephrotoxicity","volume":"211","author":"Chaim","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.toxicon.2010.08.008_bib9","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/0041-0101(94)90323-9","article-title":"ELISA for the detection of toxic antigens in experimental and clinical envenoming by Tityus serrulatus scorpion venom","volume":"32","author":"Ch\u00e1vez-Ol\u00f3rtegui","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib10","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0041-0101(97)00159-1","article-title":"ELISA for the detection of venom antigens in experimental and clinical envenoming by Loxosceles intermedia spiders","volume":"36","author":"Ch\u00e1vez-Ol\u00f3rtegui","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib11","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.toxicon.2008.08.001","article-title":"Effects of the venom and the dermonecrotic toxin LiRecDT1 of Loxosceles intermedia in the rat liver","volume":"52","author":"Christoff","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib12","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0922-4106(94)90142-2","article-title":"Depressive effects of arenobufagin on the delayed rectifier K+ current of guinea-pig cardiac myocytes","volume":"266","author":"Cruz","year":"1994","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.toxicon.2010.08.008_bib13","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.bbrc.2005.08.039","article-title":"Ceramide-1-P induces Ca2+ mobilization in Jurkat T-cells by elevation of Ins(1,4,5)-P3 and activation of a store-operated calcium channel","volume":"336","author":"Colina","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2010.08.008_bib14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0041-0101(03)00127-2","article-title":"Hematological cell findings in bone marrow and peripheral blood of rabbits after experimental acute exposure to Loxosceles intermedia (brown spider) venom","volume":"42","author":"da Silva","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib15","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.toxicon.2004.07.012","article-title":"Brown spiders and loxoscelism","volume":"44","author":"da Silva","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib16","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.biochi.2006.02.008","article-title":"Molecular cloning and functional characterization of two isoforms of dermonecrotic toxin from Loxosceles intermedia (brown spider) venom gland","volume":"88","author":"da Silveira","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2010.08.008_bib17","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.toxicon.2007.08.011","article-title":"Loxosceles venom-induced cytokine activation, hemolysis, and acute kidney injury","volume":"51","author":"de Souza","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib18","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.toxicon.2009.09.019","article-title":"A protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptide","volume":"55","author":"Dias-Lopes","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib19","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.toxicon.2006.06.019","article-title":"Functional characterization and epitope analysis of a recombinant dermonecrotic protein from Loxosceles intermedia spider","volume":"48","author":"Felicori","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib20","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1016\/j.vaccine.2009.04.038","article-title":"An in vivo protective response against toxic effects of the dermonecrotic protein from Loxosceles intermedia spider venom elicited by synthetic epitopes","volume":"27","author":"Felicori","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.toxicon.2010.08.008_bib21","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0035-9203(02)90101-X","article-title":"Rhabdomyolysis in presumed viscero-cutaneous loxoscelism: report of two cases","volume":"96","author":"Fran\u00e7a","year":"2002","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.toxicon.2010.08.008_bib22","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/0041-0101(79)90263-0","article-title":"An in vitro model for studying hemolysis associated with venom from the brown recluse spider (Loxosceles reclusa)","volume":"17","author":"Futrell","year":"1979","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib23","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/00000441-199210000-00008","article-title":"Loxoscelism","volume":"304","author":"Futrell","year":"1992","journal-title":"Am. J. Med. Sci."},{"key":"10.1016\/j.toxicon.2010.08.008_bib24","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1097\/00005344-200201000-00003","article-title":"Sphingosine modulates myocyte electrophysiology, induces negative inotropy, and decreases survival after myocardial ischemia","volume":"39","author":"Friedrichs","year":"2002","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"10.1016\/j.toxicon.2010.08.008_bib25","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1590\/S0037-86822003000300019","article-title":"First record on Loxosceles laeta (Nicolet, 1849) (Araneae, Sicariidae) in the West Zone of S\u00e3o Paulo City, S\u00e3o Paulo, Brazil, and considerations regarding its geographic distribution","volume":"36","author":"Gon\u00e7alves-de-Andrade","year":"2003","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.toxicon.2010.08.008_bib26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0041-0101(88)90215-2","article-title":"Are the toxicological effects of scorpion envenomation related to tissue venom concentration?","volume":"26","author":"Ismail","year":"1988","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib27","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1016\/S0041-0101(02)00201-5","article-title":"Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom","volume":"40","author":"Kalapothakis","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib28","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.toxicon.2007.07.001","article-title":"The Loxtox protein family in Loxosceles intermedia (Mello-Leit\u00e3o) venom","volume":"50","author":"Kalapothakis","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib29","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1085\/jgp.84.5.705","article-title":"Inactivation of calcium channel current in the calf cardiac Purkinje fiber. Evidence for voltage- and calcium-mediated mechanisms","volume":"84","author":"Kass","year":"1984","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.toxicon.2010.08.008_bib30","doi-asserted-by":"crossref","first-page":"4320","DOI":"10.1529\/biophysj.107.121046","article-title":"Membrane organization and ionization behavior of the minor but crucial lipid ceramide-1-phosphate","volume":"294","author":"Kooijman","year":"2008","journal-title":"Biophys. J."},{"key":"10.1016\/j.toxicon.2010.08.008_bib31","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1016\/j.biochi.2008.07.011","article-title":"Nephrotoxicity caused by brown spider venom phospholipase-D (dermonecrotic toxin) depends on catalytic activity","volume":"90","author":"Kusma","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2010.08.008_bib32","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.toxicon.2004.10.007","article-title":"Pharmacokinetic studies of scorpion venom before and after antivenom immunotherapy","volume":"45","author":"Krifi","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.08.008_bib33","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1016\/j.lfs.2007.06.033","article-title":"Kinin B1 receptor participates in the control of cardiac function in mice","volume":"81","author":"Lauton-Santos","year":"2007","journal-title":"Life Sci."},{"key":"10.1016\/j.toxicon.2010.08.008_bib35","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1177\/002215540405200404","article-title":"Experimental evidence for a direct cytotoxicity of Loxosceles intermedia (brown spider) venom in renal tissue","volume":"52","author":"Luciano","year":"2004","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.toxicon.2010.08.008_bib36","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1002\/pmic.200401096","article-title":"Proteome analysis of brown spider venom: identification of loxnecrogin isoforms in Loxosceles gaucho venom","volume":"5","author":"Machado","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.toxicon.2010.08.008_bib37","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1177\/22.12.1084","article-title":"Peroxidase-labeled antibody. A new method of conjugation","volume":"22","author":"Nakane","year":"1974","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.toxicon.2010.08.008_bib39","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1152\/ajpheart.00833.2006","article-title":"Abolition of reperfusion-induced arrhythmias in hearts from thiamine-deficient rats","volume":"293","author":"Oliveira","year":"2007","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.toxicon.2010.08.008_bib41","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.tox.2008.12.012","article-title":"The profound effects of microcystin on cardiac antioxidant enzymes, mitochondrial function and cardiac toxicity in rat","volume":"257","author":"Qiu","year":"2009","journal-title":"Toxicology"},{"key":"10.1016\/j.toxicon.2010.08.008_bib42","first-page":"78","article-title":"Cutaneous loxoscelism with edematous predominance","volume":"53","author":"Schenone","year":"1998","journal-title":"Bol. Chil. Parasitol."},{"key":"10.1016\/j.toxicon.2010.08.008_bib43","doi-asserted-by":"crossref","first-page":"327","DOI":"10.2170\/physiolsci.RP010107","article-title":"A simple technique for isolating healthy heart cells from mouse models","volume":"57","author":"Shioya","year":"2007","journal-title":"J. Physiol. Sci."},{"key":"10.1016\/j.toxicon.2010.08.008_bib44","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1006\/bbrc.1998.9474","article-title":"Sphingomyelinases in the venom of the spider Loxosceles intermedia are responsible for both dermonecrosis and complement-dependent hemolysis","volume":"251","author":"Tambourgi","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010110003260?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010110003260?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T21:07:50Z","timestamp":1758575270000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010110003260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["S0041010110003260"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2010.08.008","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2010.08.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}