{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:15:52Z","timestamp":1777500952598,"version":"3.51.4"},"reference-count":33,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Institutional Research and Academic Career Development Award"},{"DOI":"10.13039\/100006747","name":"San Francisco State University","doi-asserted-by":"crossref","award":["K12GM00679"],"award-info":[{"award-number":["K12GM00679"]}],"id":[{"id":"10.13039\/100006747","id-type":"DOI","asserted-by":"crossref"}]},{"name":"a Fairfield University Faculty Research Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2011,2]]},"DOI":"10.1016\/j.toxicon.2010.12.014","type":"journal-article","created":{"date-parts":[[2010,12,26]],"date-time":"2010-12-26T09:08:52Z","timestamp":1293354532000},"page":"342-347","source":"Crossref","is-referenced-by-count":19,"title":["A rapid and sensitive fluorometric method for the quantitative analysis of snake venom metalloproteases and their inhibitors"],"prefix":"10.1016","volume":"57","author":[{"given":"J.E.","family":"Biardi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.T.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Lander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Whitley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.P.","family":"Nambiar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2010.12.014_bib1","doi-asserted-by":"crossref","first-page":"11347","DOI":"10.1074\/jbc.M009725200","article-title":"Authentic matrix vesicles contain active metalloproteases (MMP): A role for matrix vesicle-associated MMP-13 in activation of transforming growth factor-beta","volume":"276","author":"D\u2019Angelo","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2010.12.014_bib2","doi-asserted-by":"crossref","unstructured":"Biardi, J.E., Coss, R.G., 2011. Rock squirrel (Spermophilus variegatus) blood sera affects proteolytic and hemolytic activities of rattlesnake venoms. Toxicon, doi:10.1016\/j.toxicon.2010.12.011.","DOI":"10.1016\/j.toxicon.2010.12.011"},{"key":"10.1016\/j.toxicon.2010.12.014_bib3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0003-9861(89)90350-0","article-title":"Degradation of extracellular matrix proteins by haemorrhagic metalloproteinases","volume":"275","author":"Baramova","year":"1989","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2010.12.014_bib4","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1016\/S0041-0101(01)00103-9","article-title":"Novel in vitro assays for assessing the haemorrhagic activity of venom metalloproteinase inhibitors","volume":"39","author":"Bee","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib5","unstructured":"Biardi, J.E. 2000. Adaptive variation and Coevolution in California ground squirrel (Spermophilus beecheyi) and rock squirrel (Spermophilus variegatus) resistance to rattlesnake venom. Ph.D. Dissertation, University of California, Davis. UMI Proquest Publication Number AAT 9969591."},{"key":"10.1016\/j.toxicon.2010.12.014_bib6","series-title":"The Biology of Rattlesnakes","first-page":"557","article-title":"The ecological and evolutionary context of mammalian resistance to rattlesnake venoms","author":"Biardi","year":"2008"},{"key":"10.1016\/j.toxicon.2010.12.014_bib7","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/S0041-0101(99)00179-8","article-title":"California ground squirrel (Spermophilus beecheyi) blood sera inhibits crotalid venom proteolytic activity","volume":"38","author":"Biardi","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib8","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s10886-006-9357-8","article-title":"California ground squirrel (Spermophilus beecheyi) defenses against rattlesnake venom digestive and hemostatic toxins","volume":"32","author":"Biardi","year":"2006","journal-title":"J. Chem. Ecol."},{"key":"10.1016\/j.toxicon.2010.12.014_bib9","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0163-7258(94)90049-3","article-title":"Haemorrhagic metalloproteinases from snake venoms","volume":"62","author":"Bjarnason","year":"1994","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.toxicon.2010.12.014_bib10","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/S0300-9084(00)01178-0","article-title":"Snake venom proteins acting on hemostasis","volume":"82","author":"Braud","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2010.12.014_bib11","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/S0041-0101(99)00205-6","article-title":"A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms","volume":"38","author":"DeRoodt","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib12","series-title":"Proteolytic Enzymes","first-page":"77","article-title":"Determination of protease mechanism","author":"Dunn","year":"2001"},{"key":"10.1016\/j.toxicon.2010.12.014_bib13","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/j.toxicon.2005.02.012","article-title":"Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases","volume":"45","author":"Fox","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib14","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1111\/j.1742-4658.2008.06466.x","article-title":"Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity","volume":"275","author":"Fox","year":"2008","journal-title":"FEBS J."},{"key":"10.1016\/j.toxicon.2010.12.014_bib15","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0003-9861(02)00447-2","article-title":"A novel prothrombin activator from the venom of Micropechis ikaheka: isolation and characterization","volume":"408","author":"Gao","year":"2002","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2010.12.014_bib16","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1016\/S0041-0101(02)00180-0","article-title":"Fluorometric assay using naphthylamide substrates for assessing novel venom peptidase activities","volume":"40","author":"Gasparello-Clemente","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib17","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0300-9084(00)01163-9","article-title":"Snake venom metalloproteases: Their role in the pathogenesis of local tissue damage","volume":"82","author":"Guti\u00e9rrez","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2010.12.014_bib18","series-title":"Handbook of Venoms and Toxins of Reptiles","first-page":"115","article-title":"Snake venom metalloproteinases: Biological roles and participation in the pathophysiology of envenomation","author":"Guti\u00e9rrez","year":"2009"},{"key":"10.1016\/j.toxicon.2010.12.014_bib19","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/abbi.1994.1026","article-title":"cDNA sequences for four snake venom metalloproteases: Structure, classification, and their relationship to mammamlian reproductive proteins","volume":"308","author":"Hite","year":"1994","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2010.12.014_bib20","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1161\/01.RES.0000022166.74073.F8","article-title":"Tyrosine kinase activity of discoidin domain receptor 1 is necessary for smooth muscle cell migration and matrix metalloproteinase expression","volume":"90","author":"Hou","year":"2002","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2010.12.014_bib21","doi-asserted-by":"crossref","first-page":"43","DOI":"10.7883\/yoken1952.13.43","article-title":"Studies on the quantitative method for determination of hemorrhagic activity of Habu snake venom","volume":"13","author":"Kondo","year":"1960","journal-title":"Jpn. J. Med. Sci. Biol."},{"key":"10.1016\/j.toxicon.2010.12.014_bib22","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/j.toxicon.2010.02.028","article-title":"Evolutionary trends in venom composition in the Western Rattlesnakes (Crotalus viridis sensu lato): Toxicity vs. tenderizers","volume":"55","author":"Mackessy","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib23","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/S0167-4838(99)00268-X","article-title":"Snake venom proteases affecting hemostasis and thrombosis","volume":"1477","author":"Matsui","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2010.12.014_bib24","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1177\/002215540305100613","article-title":"In situ localization of gelatinolytic activity in the extracellular matrix of metastases of colon cancer in rat liver using quenched fluorogenic DQ-gelatin","volume":"51","author":"Mook","year":"2003","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.toxicon.2010.12.014_bib25","doi-asserted-by":"crossref","first-page":"8464","DOI":"10.1523\/JNEUROSCI.19-19-08464.1999","article-title":"Matrix metalloproteinase-9\/gelatinase B is required for process outgrowth by oligodendrocytes","volume":"19","author":"Oh","year":"1999","journal-title":"J. Neurosci."},{"key":"10.1016\/j.toxicon.2010.12.014_bib26","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1163\/156853901316924485","article-title":"Snake-directed antipredator behavior of rock squirrels (Spermophilus variegatus): Population differences and snake-species discrimination","volume":"138","author":"Owings","year":"2001","journal-title":"Behaviour"},{"key":"10.1016\/j.toxicon.2010.12.014_bib27","series-title":"Handbook of Toxinology","first-page":"602","article-title":"Locally acting agents: Myotoxins, hemorrhagic toxins, and dermonecrotic factors","author":"Ownby","year":"1990"},{"key":"10.1016\/j.toxicon.2010.12.014_bib28","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0041-0101(84)90023-0","article-title":"A new method for quantitating hemorrhage induced by rattlesnake venoms: ability of polyvalent antivenom to neutralize hemorrhagic activities","volume":"22","author":"Ownby","year":"1984","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib30","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.bbamcr.2009.04.010","article-title":"Structural and functional bases for allosteric control of MMP activities: can it pave the path for selective inhibition?","volume":"1803","author":"Sela-Passwell","year":"2010","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2010.12.014_bib31","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/0041-0101(88)90328-5","article-title":"Proteolytic, hemorrhagic and hemolytic activities of snake venoms","volume":"26","author":"Soto","year":"1988","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2010.12.014_bib32","first-page":"949","article-title":"Development of simple standard assay procedures for the characterization of snake venoms","volume":"61","author":"Theakston","year":"1983","journal-title":"Bull. WHO"},{"key":"10.1016\/j.toxicon.2010.12.014_bib33","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.gene.2006.03.008","article-title":"Venom gland EST analysis of the saw-scaled viper, Echis ocellatus, reveals novel \u03b19\u03b21 integrin-binding motifs in venom metalloproteinases and a new group of putative toxins, renin-like aspartic proteases","volume":"377","author":"Wagstaff","year":"2006","journal-title":"Gene"},{"key":"10.1016\/j.toxicon.2010.12.014_bib34","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1210\/jcem.87.5.8487","article-title":"In vivo evidence for active matrix metalloproteinases in human endometrium supports their role in tissue breakdown at menstruation","volume":"87","author":"Zhang","year":"2002","journal-title":"J. Clin. Endocrin. Metab."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010110004459?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010110004459?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T09:18:05Z","timestamp":1559899085000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010110004459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["S0041010110004459"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2010.12.014","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2011,2]]}}}