{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:30:47Z","timestamp":1791401447227,"version":"4.3.3"},"reference-count":43,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2012,9]]},"DOI":"10.1016\/j.toxicon.2012.04.347","type":"journal-article","created":{"date-parts":[[2012,5,7]],"date-time":"2012-05-07T21:19:40Z","timestamp":1336425580000},"page":"348-357","source":"Crossref","is-referenced-by-count":37,"title":["Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA"],"prefix":"10.1016","volume":"60","author":[{"given":"Takeshi","family":"Tsumuraya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsutoshi","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuo","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Hirama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2012.04.347_bib1","first-page":"412","article-title":"Ciguatera in French Polynesian islands of coral, fish, and men","volume":"85","author":"Bagnis","year":"1992","journal-title":"Bull. Soc. Pathol. Exot."},{"key":"10.1016\/j.toxicon.2012.04.347_bib2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0041-0101(98)00169-X","article-title":"Ciguatoxins and brevetoxins, neurotoxic polyether compounds active on sodium channels","volume":"37","author":"Dechraoui","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.toxicon.2009.09.008","article-title":"Ciguatera: a public health perspective","volume":"56","author":"Dickey","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib4","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0022-1759(85)90044-4","article-title":"Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay","volume":"77","author":"Friguet","year":"1985","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.toxicon.2012.04.347_bib5","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.1126\/science.1065757","article-title":"Total synthesis of ciguatoxin CTX3C","volume":"294","author":"Hirama","year":"2001","journal-title":"Science"},{"key":"10.1016\/j.toxicon.2012.04.347_bib6","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0041-0101(77)90014-9","article-title":"A Radioimmunoassay for the detection of ciguatoxin","volume":"15","author":"Hokama","year":"1977","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib7","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/jcla.1860060111","article-title":"Cross-reactivity of highly purified okadaic acid (OA), synthetic, spiroketal east sphere of OA and ciguatoxin","volume":"6","author":"Hokama","year":"1992","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.toxicon.2012.04.347_bib8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1080\/02652039309374132","article-title":"Immunological studies using monoclonal antibodies for detection of low dalton marine toxins","volume":"10","author":"Hokama","year":"1993","journal-title":"Food Addit. Contam."},{"key":"10.1016\/j.toxicon.2012.04.347_bib9","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1093\/jaoac\/81.4.727","article-title":"Simple membrane immunobead assay for detecting ciguatoxin and related polyethers from human ciguatera intoxication and natural reef fishes","volume":"81","author":"Hokama","year":"1998","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.toxicon.2012.04.347_bib10","first-page":"120","article-title":"First- and second-generation total synthesis of ciguatoxin CTX3C","volume":"191","author":"Inoue","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.toxicon.2012.04.347_bib11","doi-asserted-by":"crossref","first-page":"9352","DOI":"10.1021\/ja063041p","article-title":"Total synthesis of ciguatoxin and 51-hydroxyCTX3C","volume":"128","author":"Inoue","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.toxicon.2012.04.347_bib12","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.toxicon.2009.02.017","article-title":"Use of monoclonal antibodies as an effective strategy for treatment of ciguatera poisoning","volume":"53","author":"Inoue","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib13","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1021\/ol0364475","article-title":"Synthesis of the fully functionalized ABCDE ring moiety of ciguatoxin","volume":"6","author":"Kobayashi","year":"2004","journal-title":"Org. Lett."},{"key":"10.1016\/j.toxicon.2012.04.347_bib14","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1002\/(SICI)1521-3765(20000502)6:9<1656::AID-CHEM1656>3.3.CO;2-1","article-title":"Antibody-catalyzed removal of the p-nitrobenzyl ester protecting group: the molecular basis of the broad substrate specificity","volume":"6","author":"Kurihara","year":"2000","journal-title":"Chem. Eur. J."},{"key":"10.1016\/j.toxicon.2012.04.347_bib15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0168-1605(00)00382-2","article-title":"Ciguatera: recent abdances but the risk remains","volume":"61","author":"Lehane","year":"2000","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.toxicon.2012.04.347_bib16","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/0041-0101(91)90209-A","article-title":"Purification and characterization of ciguatoxins from moray eel (Lycodontis javanicus, Muraenidae)","volume":"29","author":"Lewis","year":"1991","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0041-0101(96)00132-8","article-title":"Characterization of ciguatoxins and ciguatoxin congeners present in ciguateric fish by gradient reverse-phase high performance liquid chromatography\/mass spectroscopy","volume":"35","author":"Lewis","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib18","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1021\/ac980598h","article-title":"HPLC\/tandem electrospray mass spectroscopy for the determination of sub-ppb levels of Pacific and Caribbean ciguatoxins in crude extracts of fish","volume":"72","author":"Lewis","year":"1999","journal-title":"Anal. Chem."},{"key":"10.1016\/j.toxicon.2012.04.347_bib19","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0041-0101(00)00161-6","article-title":"The changing face of ciguatera","volume":"39","author":"Lewis","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib20","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.toxicon.2009.03.013","article-title":"Rapid extraction combined with LC-tandem mass spectrometry (CERM-LC\/MS\/MS) for the determination of ciguatoxins in ciguateric fish flesh","volume":"54","author":"Lewis","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib21","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1093\/jaoac\/78.2.521","article-title":"Detection of sodium channel toxins: directed cytotoxicity assays of purified ciguatoxins, brevetoxins, saxitoxins, and seafood extracts","volume":"78","author":"Manger","year":"1995","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.toxicon.2012.04.347_bib22","doi-asserted-by":"crossref","first-page":"4380","DOI":"10.1021\/ja00167a040","article-title":"Structures and configurations of ciguatoxin from the moray eel Gymnothorax javanicus and its likely precursor from the dinoflagellate Gambierdiscus toxicus","volume":"112","author":"Murata","year":"1990","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.toxicon.2012.04.347_bib23","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1016\/S0960-894X(01)00358-4","article-title":"Concise synthesis of ciguatoxin ABC-ring fragments and surface plasmon resonance study of the interaction of their BSA conjugates with monoclonal antibodies","volume":"15","author":"Nagumo","year":"2001","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.toxicon.2012.04.347_bib24","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.jim.2004.04.003","article-title":"Phage-display selection of antibodies to the left end of CTX3C using synthetic fragments","volume":"289","author":"Nagumo","year":"2004","journal-title":"J. Immunol. Methods"},{"issue":"Special Issue","key":"10.1016\/j.toxicon.2012.04.347_bib25","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1055\/s-1999-3646","article-title":"Designed hapten aimed at anti-ciguatoxin monoclonal antibody: synthesis, immunization and discrimination of the C2 configuration","author":"Oguri","year":"1999","journal-title":"Synthesis"},{"key":"10.1016\/j.toxicon.2012.04.347_bib26","doi-asserted-by":"crossref","first-page":"7608","DOI":"10.1021\/ja034990a","article-title":"Synthesis-based approach toward direct sandwich immunoassay for ciguatoxin CTX3C","volume":"125","author":"Oguri","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.toxicon.2012.04.347_bib27","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.1021\/ac100516q","article-title":"First toxin profile of ciguateric fish in Madeira Arquipelago (Euroupe)","volume":"82","author":"Otero","year":"2010","journal-title":"Anal. Chem."},{"key":"10.1016\/j.toxicon.2012.04.347_bib28","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/S0041-0101(99)00183-X","article-title":"Characterization of mice antisera elicited with a ciguatoxin tetracyclic synthetic ring fragment (JKLM) conjugated to carrier proteins","volume":"38","author":"Pauillac","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib29","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/S0041-0101(96)00166-3","article-title":"Identification of Caribbean ciguatoxins as the cause of an outbreak of fish poisoning among U. S. soldiers in Haiti","volume":"35","author":"Poli","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib30","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1016\/S0040-4039(00)91978-6","article-title":"The structure of CTX3C, a ciguatoxin congener isolated from cultured Gambierdiscus toxicus","volume":"34","author":"Satake","year":"1993","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.toxicon.2012.04.347_bib31","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/S0040-4039(97)10808-5","article-title":"Isolation and structures of new ciguatoxin analogs, 2,3-dihydroxyCTX3C and 51-hydroxyCTX3C, accumulated in tropical reef fish","volume":"39","author":"Satake","year":"1998","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.toxicon.2012.04.347_bib32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0040-4020(01)80733-X","article-title":"Ciguatera and its off-shoots chance encounters en route to a molecular structure","volume":"50","author":"Scheuer","year":"1994","journal-title":"Tetrahedron"},{"key":"10.1016\/j.toxicon.2012.04.347_bib33","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1021\/ja00081a008","article-title":"Esterolytic antibodies induced to haptens with a 1,2-amino alcohol functionality","volume":"116","author":"Suga","year":"1994","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.toxicon.2012.04.347_bib34","doi-asserted-by":"crossref","first-page":"3748","DOI":"10.1002\/1521-3765(20010903)7:17<3748::AID-CHEM3748>3.0.CO;2-G","article-title":"Catalytic antibodies induced by a zwitterionic hapten","volume":"7","author":"Tsumuraya","year":"2001","journal-title":"Chem. Eur. J."},{"key":"10.1016\/j.toxicon.2012.04.347_bib35","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.toxicon.2006.05.014","article-title":"Production of monoclonal antibodies for sandwich immunoassay detection of ciguatoxin 51-hydoxyCTX3C","volume":"48","author":"Tsumuraya","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib36","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.toxicon.2009.06.003","article-title":"Production of monoclonal antibodies for sandwich immunoassay detection of Pacific ciguatoxins","volume":"56","author":"Tsumuraya","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib37","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.toxicon.2005.06.023","article-title":"Study of an outbreak of ciguatera fish poisoning in Hong Kong","volume":"46","author":"Wong","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.04.347_bib38","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1021\/np100729d","article-title":"Stereroselective 6-oxo radical cyclization using cis-viniy sulfoxide: practical total synthesis of CTX3C","volume":"74","author":"Yamashita","year":"2011","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.toxicon.2012.04.347_bib39","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/jaoac\/78.2.574","article-title":"Determination of marine toxins in foods","volume":"78","author":"Yasumoto","year":"1995","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.toxicon.2012.04.347_bib40","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1021\/cr00021a011","article-title":"Marine toxins","volume":"93","author":"Yasumoto","year":"1993","journal-title":"Chem. Rev."},{"key":"10.1016\/j.toxicon.2012.04.347_bib41","doi-asserted-by":"crossref","first-page":"4988","DOI":"10.1021\/ja9944204","article-title":"Structural elucidation of ciguatoxin congeners by fast-atom bombardment tandem mass spectroscopy","volume":"122","author":"Yasumoto","year":"2000","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.toxicon.2012.04.347_bib42","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/tcr.1010","article-title":"The chemistry and biological function of natural marine toxins","volume":"1","author":"Yasumoto","year":"2001","journal-title":"Chem. Rec."},{"key":"10.1016\/j.toxicon.2012.04.347_bib43","doi-asserted-by":"crossref","first-page":"8886","DOI":"10.1021\/ac200799j","article-title":"Detailed LC-MS\/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific","volume":"83","author":"Yogi","year":"2011","journal-title":"Anal. Chem."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112004540?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112004540?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T07:26:59Z","timestamp":1594106819000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010112004540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["S0041010112004540"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2012.04.347","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2012,9]]}}}