{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:49:19Z","timestamp":1788871759668,"version":"build-2803163510"},"reference-count":28,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T00:00:00Z","timestamp":1374451200000},"content-version":"vor","delay-in-days":294,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation \u2013 FAPESP","award":["09\/02299-8"],"award-info":[{"award-number":["09\/02299-8"]}]},{"name":"S\u00e3o Paulo Research Foundation \u2013 FAPESP","award":["2010\/19916-7"],"award-info":[{"award-number":["2010\/19916-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2012,10]]},"DOI":"10.1016\/j.toxicon.2012.06.010","type":"journal-article","created":{"date-parts":[[2012,6,29]],"date-time":"2012-06-29T20:15:48Z","timestamp":1341000948000},"page":"773-781","source":"Crossref","is-referenced-by-count":22,"title":["LmrTX, a basic PLA2 (D49) purified from Lachesis muta rhombeata snake venom with enzymatic-related antithrombotic and anticoagulant activity"],"prefix":"10.1016","volume":"60","author":[{"given":"Daniela C.S.","family":"Damico","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Vassequi-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.D.","family":"Torres-Huaco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.C.","family":"Nery-Diez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.C.G.","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.L.","family":"Da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.P.","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.B.","family":"Mendes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Antunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.C.","family":"Werneck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio","family":"Marangoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2012.06.010_bib1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/0041-0101(95)00159-X","article-title":"Purification and partial characterization of a thrombin-like\/gyroxin enzyme from bushmaster (Lachesis rhombeata) venom","volume":"34","author":"Aguiar","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib2","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/S0300-9084(00)01150-0","article-title":"Myotoxic phospholipases A2 in bothrops snake venoms: effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu","volume":"82","author":"Andri\u00e3o-Escarso","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2012.06.010_bib3","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/0041-0101(96)00036-0","article-title":"Phospholipase A2 \u2013 a structural review","volume":"34","author":"Arni","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib4","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1023\/A:1007152303179","article-title":"Enzymatic characterization of a novel phospholipase A2 from Crotalus durissus cascavella rattlesnake (maracamb\u00f3ia) venom","volume":"19","author":"Beghini","year":"2000","journal-title":"J. Protein Chem."},{"key":"10.1016\/j.toxicon.2012.06.010_bib5","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0041-0101(97)00051-2","article-title":"The three dimensional structures of two toxins from snake venom throw light on the anticoagulant and neurotoxic sites of phospholipase A2","volume":"36","author":"Carredano","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib6","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0076-6879(91)97134-K","article-title":"Chromogenic substrates and assay of phospholipases A2","volume":"197","author":"Cho","year":"1991","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.toxicon.2012.06.010_bib7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.bbagen.2005.05.022","article-title":"Biochemical and enzymatic characterization of two basic Asp49 phospholipase A2 isoforms from Lachesis muta muta (Surucucu) venom","volume":"1726","author":"Damico","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2012.06.010_bib8","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s10930-008-9141-4","article-title":"Purification and complete primary structure of the first PLA2 from Lachesis stenophrys (the Central American Bushmaster) snake venom","volume":"27","author":"de Assis","year":"2008","journal-title":"Protein J."},{"key":"10.1016\/j.toxicon.2012.06.010_bib9","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0041-0101(96)00128-6","article-title":"Chemical modification of histidine and lysine residues of miotoxic phospholipases A2 isoloated form Bothrops asper and Bothrops godmani snake venoms: effects on enzymatic and pharmacological properties","volume":"35","author":"Diaz-Oreiro","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib10","first-page":"84","article-title":"Estudo retrospectivo da evolu\u00e7\u00e3o dos acidentes botr\u00f3picos","volume":"31","author":"Fran\u00e7a","year":"1989","journal-title":"Rev. Inst. Med. Trop."},{"key":"10.1016\/j.toxicon.2012.06.010_bib11","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1016\/S1357-2725(03)00129-8","article-title":"Myotoxicity induced by an acidic Asp-49 phospholipase A2 isolated from Lachesis muta snake venom. Comparison with lysophosphatidylcholine","volume":"35","author":"Fuly","year":"2003","journal-title":"Int. J. Biochem. Cell. Biol."},{"issue":"2","key":"10.1016\/j.toxicon.2012.06.010_bib12","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s00239-008-9067-7","article-title":"Rapid evolution by positive selection and gene gain and loss: PLA(2) venom genes in closely related Sistrurus rattlesnakes with divergent diets","volume":"66","author":"Gibbs","year":"2008","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.toxicon.2012.06.010_bib13","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1023\/A:1026372018547","article-title":"Purification, properties, and N-Terminal amino acid sequence of a kallikrein-like enzyme from the venom of Lachesis muta rhombeata (Bushmaster)","volume":"16","author":"Giovanni-De-Simone","year":"1997","journal-title":"J. Protein Chem."},{"key":"10.1016\/j.toxicon.2012.06.010_bib14","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.jchromb.2006.02.036","article-title":"Simple affinity chromatographic procedure to purify beta-galactoside binding lectins","volume":"838","author":"Giovanni-De-Simone","year":"2006","journal-title":"Chromatogr. B Analyt. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.toxicon.2012.06.010_bib15","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0003-2697(84)90307-5","article-title":"Amino acid analysis by reverse phase high performance liquid chromatography: precolumn derivatization with phenylthiocyanate","volume":"136","author":"Henrikson","year":"1984","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2012.06.010_bib16","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/0041-0101(96)00057-8","article-title":"An aqueous endpoint assay of snake venom phospholipase A2","volume":"34","author":"Holzer","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib17","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/S0041-0101(96)00142-0","article-title":"Snakebite by the bushmaster (Lachesis muta) in Brazil: case report and review of the literature","volume":"35","author":"Jorge","year":"1997","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib18","series-title":"Venom Phospholipase A2 Enzymes: Structure, Function and Mechanism","first-page":"369","article-title":"Effects of Phospholipase A2 enzymes pn platelet aggregation","author":"Kini","year":"1997"},{"key":"10.1016\/j.toxicon.2012.06.010_bib19","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.toxicon.2005.02.018","article-title":"Structure\u2013function relationships and mechanism of anticoagulant phospholipase A2 enzymes from snake venoms","volume":"45","author":"Kini","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib20","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1590\/S0036-46651994000100012","article-title":"Inefic\u00e1cia do antiveneno Botr\u00f3pico na neutraliza\u00e7\u00e3o da atividade coagulante do veneno de Lachesis muta muta; Relato de caso e comprova\u00e7\u00e3o experimental","volume":"36","author":"Ramza","year":"1994","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"10.1016\/j.toxicon.2012.06.010_bib21","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/S0041-0101(98)00268-2","article-title":"Characterization of the local tissue damage induced by LHF-II, a metalloproteinase with weak hemorrhagic activity isolated from Lachesis muta muta snake venom","volume":"37","author":"Rucavado","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib22","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1006\/jmbi.2001.4550","article-title":"Sequence and crystal structure determination of a basic phospholipase A2 from common krait (Bungarus caeruleus) at 2.4 A\u00b0 resolution: identification and characterization of its pharmacological sites","volume":"307","author":"Singh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2012.06.010_bib23","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.toxicon.2003.11.004","article-title":"Chemical modifications of phospholipases A2 from snake venoms: effects on catalytic and pharmacological properties","volume":"42","author":"Soares","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib24","first-page":"105","article-title":"The enigma of the north margin of the Amazon River","volume":"42","author":"Souza","year":"2007","journal-title":"Bull. Chicago Herpetological Soc."},{"key":"10.1016\/j.toxicon.2012.06.010_bib25","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.toxicon.2008.08.022","article-title":"Expression of mRNAs coding for VAP1\/crotastatin-like metalloproteases in the venom glands of three South American pit vipers assessed by quantitative real-time PCR","volume":"52","author":"Tavares","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.06.010_bib26","doi-asserted-by":"crossref","first-page":"4137","DOI":"10.1182\/blood-2007-07-101733","article-title":"Mice lacking the extracellular matrix protein MAGP1 display delayed thrombotic occlusion following vessel injury","volume":"111","author":"Werneck","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.toxicon.2012.06.010_bib27","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1111\/j.1095-8312.1997.tb01634.x","article-title":"Phylogeography of the bushmaster (Lachesis muta: Viperidae): implications for neotropical biogeography, systematics, and conservation","volume":"62","author":"Zamudio","year":"1997","journal-title":"Biol. J. Linn. Soc."},{"key":"10.1016\/j.toxicon.2012.06.010_bib28","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/S0041-0101(99)00193-2","article-title":"Structure of basic phospholipase A2 from Agkistrodon halys Pallas: implications for its association, hemolytic and anticoagulant activities","volume":"38","author":"Zhao","year":"2000","journal-title":"Toxicon"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112005211?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112005211?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,11,22]],"date-time":"2018-11-22T07:21:14Z","timestamp":1542871274000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010112005211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["S0041010112005211"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2012.06.010","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2012,10]]}}}