{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:04:16Z","timestamp":1780938256968,"version":"3.54.1"},"reference-count":32,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"DGAPA-UNAM IN230203"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2012,12]]},"DOI":"10.1016\/j.toxicon.2012.08.018","type":"journal-article","created":{"date-parts":[[2012,9,5]],"date-time":"2012-09-05T16:01:13Z","timestamp":1346860873000},"page":"1223-1227","source":"Crossref","is-referenced-by-count":23,"title":["Identification, cDNA cloning and heterologous expression of a hyaluronidase from the tarantula Brachypelma vagans venom"],"prefix":"10.1016","volume":"60","author":[{"given":"Herlinda","family":"Clement","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Olvera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mabel","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Zamudio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Palomares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George V.","family":"Odell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Alag\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana","family":"S\u00e1nchez-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2012.08.018_bib1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.pep.2011.10.009","article-title":"Production of disulfide-bonded proteins in Escherichia coli","volume":"82","author":"Berkmen","year":"2012","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2012.08.018_bib2","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/0041-0101(92)90392-I","article-title":"Molecular mass determination and assay of venom hyaluronidases by sodium dodecyl sulphate-polyacrylamide gel electrophoresis","volume":"30","author":"Cevallos","year":"1992","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.08.018_bib3","doi-asserted-by":"crossref","first-page":"6911","DOI":"10.1021\/bi700382g","article-title":"Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis","volume":"46","author":"Chao","year":"2007","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2012.08.018_bib4","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1089\/ars.2010.3656","article-title":"The folding of disulfide-rich proteins","volume":"14","author":"Craik","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"10.1016\/j.toxicon.2012.08.018_bib5","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.toxicon.2006.11.024","article-title":"Hyaluronidases in Loxosceles intermedia (Brown spider) venom are endo-beta-N-acetyl-d-hexosaminidases hydrolases","volume":"49","author":"da Silveira","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.08.018_bib6","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.bbagen.2007.04.006","article-title":"Four disulfide-bridged scorpion beta neurotoxin CssII: heterologous expression and proper folding in\u00a0vitro","volume":"1770","author":"Estrada","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2012.08.018_bib7","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/S1472-6483(10)60030-2","article-title":"Human recombinant hyaluronidase (Cumulase) improves intracytoplasmic sperm injection survival and fertilization rates","volume":"18","author":"Evison","year":"2009","journal-title":"Reprod. Biomed."},{"key":"10.1016\/j.toxicon.2012.08.018_bib8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1517\/17425247.4.4.427","article-title":"Recombinant human hyaluronidase (rHuPH20): an enabling platform for subcutaneous drug and fluid administration","volume":"4","author":"Frost","year":"2007","journal-title":"Expert Opin. Drug Deliv."},{"key":"10.1016\/j.toxicon.2012.08.018_bib9","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1073\/pnas.90.8.3569","article-title":"Bee venom hyaluronidase is homologous to a membrane protein of mammalian sperm","volume":"90","author":"Gmachl","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.toxicon.2012.08.018_bib10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.biochi.2004.02.004","article-title":"Isolation and characterization of hyaluronidase a \u201cspreading factor\u201d from Indian cobra (Naja naja) venom","volume":"86","author":"Girish","year":"2004","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2012.08.018_bib11","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1016\/j.lfs.2007.02.037","article-title":"The magic glue hyaluronan and its eraser hyaluronidase: a biological overview","volume":"80","author":"Girish","year":"2007","journal-title":"Life Sci."},{"key":"10.1016\/j.toxicon.2012.08.018_bib12","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0305-0491(76)90087-0","article-title":"The comparative biochemistry of invertebrate mucopolysaccharides: insecta (Calliphora erytrocephala)","volume":"53","author":"Hoglund","year":"1976","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.toxicon.2012.08.018_bib13","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/cbf.1261","article-title":"Snake venom hyaluronidase: a therapeutic target","volume":"24","author":"Kemparaju","year":"2006","journal-title":"Cell. Biochem. Funct."},{"key":"10.1016\/j.toxicon.2012.08.018_bib14","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1002\/pro.5560040902","article-title":"Hyaluronidases-A group of neglected enzymes","volume":"4","author":"Kreil","year":"1995","journal-title":"Protein Sci."},{"key":"10.1016\/j.toxicon.2012.08.018_bib15","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1111\/j.1524-4725.2010.01585.x","article-title":"Hyaluronidase","volume":"36","author":"Lee","year":"2010","journal-title":"Dermatol. Surg."},{"key":"10.1016\/j.toxicon.2012.08.018_bib16","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2012.08.018_bib17","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1385\/MB:13:2:153","article-title":"Generation of baculovirus expression vectors","volume":"13","author":"Luque","year":"1999","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.toxicon.2012.08.018_bib18","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.toxicon.2011.07.014","article-title":"Properties and cDNA cloning of a hyaluronidase from the stonefish Synanceia verrucosa venom","volume":"58","author":"Madokoro","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.08.018_bib19","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.jbiotec.2005.10.005","article-title":"Intracellular distribution of rotavirus structural proteins and virus-like particles expressed in the insect cell-baculovirus system","volume":"122","author":"Mena","year":"2006","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.toxicon.2012.08.018_bib20","first-page":"1404","article-title":"Mining invertebrate natural products for future therapeutic treasure","volume":"6","author":"Park","year":"2011","journal-title":"Nat. Prod. Commun."},{"key":"10.1016\/j.toxicon.2012.08.018_bib21","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.toxicon.2011.09.015","article-title":"Scorpion and spider venom peptides: gene cloning and peptide expression","volume":"58","author":"Quintero-Hern\u00e1ndez","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.08.018_bib22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1096\/fasebj.10.1.8566547","article-title":"In\u00a0vitro folding of inclusion body proteins","volume":"10","author":"Rainer","year":"1996","journal-title":"FASEB J."},{"key":"10.1016\/j.toxicon.2012.08.018_bib23","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1093\/bioinformatics\/btm637","article-title":"Predicting disulfide bond connectivity in proteins by correlated mutations analysis","volume":"24","author":"Rubinstein","year":"2008","journal-title":"Bioinformatics"},{"key":"10.1016\/j.toxicon.2012.08.018_bib24","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1473-2165.2009.00462.x","article-title":"Hyaluronidase in the correction of hyaluronic acid-based fillers: a review and a recommendation for use","volume":"8","author":"Rzany","year":"2009","journal-title":"J. Cosmet. Dermatol."},{"key":"10.1016\/j.toxicon.2012.08.018_bib25","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1002\/biot.201000335","article-title":"Tuned Escherichia coli as a host for the expression of disulfide-rich proteins","volume":"6","author":"Salinas","year":"2011","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.toxicon.2012.08.018_bib26","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/S0091-6749(98)70179-4","article-title":"Superior biologic activity of the recombinant bee venom allergen hyaluronidase expressed in baculovirus-infected insect cells as compared with Escherichia coli","volume":"101","author":"Soldatova","year":"1998","journal-title":"J. Allergy Clin. Inmmunol."},{"key":"10.1016\/j.toxicon.2012.08.018_bib27","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1021\/cr050247k","article-title":"Hyaluronidases: their genomics, structures and mechanisms of action","volume":"106","author":"Stern","year":"2006","journal-title":"Chem. Rev."},{"key":"10.1016\/j.toxicon.2012.08.018_bib28","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/0305-0491(83)90086-X","article-title":"Characterization of lizard venom hyaluronidase and evidence for its action as a spreading factor","volume":"76","author":"Tu","year":"1983","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.toxicon.2012.08.018_bib29","first-page":"32","article-title":"Severe acute local reactions to a hyaluronic acid-derived dermal filler","volume":"3","author":"van Dyke","year":"2010","journal-title":"J. Clin. Aesthet. Dermatol."},{"key":"10.1016\/j.toxicon.2012.08.018_bib30","doi-asserted-by":"crossref","first-page":"e294","DOI":"10.1371\/journal.pntd.0000294","article-title":"Hyaluronidase of bloodsucking insects and its enhancing effect on Leishmania infection in mice","volume":"2","author":"Volfova","year":"2008","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2012.08.018_bib31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2012.01.009","article-title":"Venom evolution through gene duplications","volume":"496","author":"Wong","year":"2012","journal-title":"Gene"},{"key":"10.1016\/j.toxicon.2012.08.018_bib32","series-title":"Enzymes Related Biochemicals Manual","author":"Wothington","year":"1993"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112007313?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112007313?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T09:15:42Z","timestamp":1562145342000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010112007313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["S0041010112007313"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2012.08.018","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}