{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:14:48Z","timestamp":1789989288114,"version":"4.0.1"},"reference-count":30,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"IZKF M\u00fcnster (U.K. and L.F.)","award":["Kih1\/020\/07"],"award-info":[{"award-number":["Kih1\/020\/07"]}]},{"name":"DFG","award":["FA 413\/3-1"],"award-info":[{"award-number":["FA 413\/3-1"]}]},{"name":"DFG","award":["SFB656 A8"],"award-info":[{"award-number":["SFB656 A8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2012,12]]},"DOI":"10.1016\/j.toxicon.2012.10.008","type":"journal-article","created":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T15:03:01Z","timestamp":1350918181000},"page":"1415-1427","source":"Crossref","is-referenced-by-count":5,"title":["Gene construction, expression and functional testing of an inotropic peptide from the venom of the black scorpion Hottentotta judaicus"],"prefix":"10.1016","volume":"60","author":[{"given":"M.A.","family":"Tekook","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Fabritz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Kirchhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"K\u00f6nig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.U.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Schmitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Zlotkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Kirchhefer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2012.10.008_bib1","first-page":"60","article-title":"Low atrial rhythm as a complication of Buthotus judaicus (black scorpion) envenomation","volume":"5","author":"Basis","year":"2005","journal-title":"Isr. J. Emerg. Med."},{"key":"10.1016\/j.toxicon.2012.10.008_bib2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/elps.1150080203","article-title":"Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels","volume":"8","author":"Blum","year":"1987","journal-title":"Electrophoresis"},{"key":"10.1016\/j.toxicon.2012.10.008_bib3","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.pep.2008.01.008","article-title":"Codon optimization can improve expression of human genes in Escherichia coli: a multi-gene study","volume":"59","author":"Burgess-Brown","year":"2008","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2012.10.008_bib4","doi-asserted-by":"crossref","first-page":"247","DOI":"10.2174\/092986607780090883","article-title":"High-level expression and purification of an analgesic peptide from Buthus martensii Karch","volume":"14","author":"Cao","year":"2007","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.toxicon.2012.10.008_bib5","doi-asserted-by":"crossref","first-page":"S15","DOI":"10.1152\/physrev.1992.72.suppl_4.S15","article-title":"Cellular and molecular biology of voltage-gated sodium channels","volume":"72","author":"Catterall","year":"1992","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.toxicon.2012.10.008_bib6","doi-asserted-by":"crossref","first-page":"8206","DOI":"10.1074\/jbc.M307531200","article-title":"Dissection of the functional surface of an anti-insect excitatory toxin illuminates a putative \u201chot spot\u201d common to all scorpion \u03b2-toxins affecting Na+ channels","volume":"279","author":"Cohen","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2012.10.008_bib7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.pep.2012.02.001","article-title":"Recombinant expression, purification, and characterization of scorpion toxin BmaTX14","volume":"82","author":"Dai","year":"2012","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2012.10.008_bib8","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1475-2859-8-26","article-title":"Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli","volume":"8","author":"De Marco","year":"2009","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.toxicon.2012.10.008_bib9","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.bbagen.2007.04.006","article-title":"Four disulfide-bridged scorpion beta neurotoxin CssII: heterologous expression and proper folding in\u00a0vitro","volume":"1770","author":"Estrada","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2012.10.008_bib10","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0022-1759(88)90224-4","article-title":"Coupling redox and enzymic reactions improves the sensitivity of the ELISA-spot assay","volume":"107","author":"Franci","year":"1988","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.toxicon.2012.10.008_bib11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.pep.2004.06.038","article-title":"Expression and purification of the BmK Mm2 neurotoxin from the scorpion Buthus martensii Karsch and its biological activity test","volume":"38","author":"Fu","year":"2004","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2012.10.008_bib12","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1007\/s10529-005-2514-2","article-title":"Synthesis, expression and purification of a type of chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, and its acute toxicity analysis","volume":"27","author":"Fu","year":"2005","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.toxicon.2012.10.008_bib13","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.toxicon.2009.02.011","article-title":"Heterologous expression of a gene that codes for Pg8, a scorpion toxin of Parabuthus granulatus, capable of generating protecting antibodies in mice","volume":"53","author":"Garcia-Gomez","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.10.008_bib14","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0378-1119(89)90359-4","article-title":"Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension","volume":"77","author":"Horton","year":"1989","journal-title":"Gene"},{"key":"10.1016\/j.toxicon.2012.10.008_bib15","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/0958-1669(95)80082-4","article-title":"Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli","volume":"6","author":"Kane","year":"1995","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.toxicon.2012.10.008_bib16","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0008-6363(03)00432-2","article-title":"Impaired relaxation in transgenic mice overexpressing junction","volume":"59","author":"Kirchhefer","year":"2003","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.toxicon.2012.10.008_bib17","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.cardiores.2004.01.005","article-title":"Transgenic triadin 1 overexpression alters SR Ca2+ handling and leads to a blunted contractile response to \u03b2-adrenergic agonists","volume":"62","author":"Kirchhefer","year":"2004","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.toxicon.2012.10.008_bib18","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2012.10.008_bib19","doi-asserted-by":"crossref","first-page":"635","DOI":"10.2174\/0929866054696172","article-title":"Expression, renaturation and functional analysis of an excitatory insect-specific toxin from scorpion Buthus martensii Karsch","volume":"12","author":"Li","year":"2005","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.toxicon.2012.10.008_bib20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1385\/MB:24:1:21","article-title":"Cloning, co-expression with an amidating enzyme, and activity of the scorpion toxin BmK ITa1 cDNA in insect cells","volume":"24","author":"Liu","year":"2003","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.toxicon.2012.10.008_bib21","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/S0041-0101(96)00092-X","article-title":"Synthesis and expression of the gene coding for noxiustoxin, a K+ channel-blocking peptide from the venom of the scorpion Centruroides noxius","volume":"34","author":"Martinez","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.10.008_bib22","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.toxicon.2011.02.001","article-title":"The tale of a resting gland: transcriptome of a replete venom gland from the scorpion Hottentotta judaicus","volume":"57","author":"Morgenstern","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.10.008_bib23","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.toxicon.2011.09.015","article-title":"Scorpion and spider venom peptides: gene cloning and peptide expression","volume":"58","author":"Quintero-Hernandez","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.10.008_bib24","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0076-6879(90)85008-C","article-title":"Use of T7 RNA polymerase to direct expression of cloned genes","volume":"185","author":"Studier","year":"1990","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.toxicon.2012.10.008_bib25","unstructured":"Tal, T., 2007. Cardiotonic Polypeptides Derived from the Venom of the Judean Black Scorpion Buthotus judaicus. Ph.D. thesis, The Hebrew University of Jerusalem."},{"key":"10.1016\/j.toxicon.2012.10.008_bib26","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s00253-006-0465-8","article-title":"Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems","volume":"72","author":"Terpe","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.toxicon.2012.10.008_bib27","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/prep.1997.0724","article-title":"In\u00a0vitro folding and functional analysis\u00a0of an anti-insect selective scorpion depressant neurotoxin produced in Escherichia coli","volume":"10","author":"Turkov","year":"1997","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2012.10.008_bib28","doi-asserted-by":"crossref","first-page":"10215","DOI":"10.1021\/bi9528309","article-title":"Functional expression and genetic alteration of an alpha scorpion neurotoxin","volume":"35","author":"Zilberberg","year":"1996","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2012.10.008_bib29","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/0041-0101(82)90235-5","article-title":"The chemistry and axonal action of two insect toxins derived from the venom of the scorpion Buthotus judaicus","volume":"20","author":"Zlotkin","year":"1982","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2012.10.008_bib30","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/0003-9861(85)90098-0","article-title":"An excitatory and a depressant insect toxin from scorpion venom both affect sodium conductance and possess a common binding site","volume":"240","author":"Zlotkin","year":"1985","journal-title":"Arch. Biochem. Biophys."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112007726?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010112007726?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T21:57:06Z","timestamp":1562277426000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010112007726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["S0041010112007726"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2012.10.008","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}