{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T10:06:31Z","timestamp":1783850791267,"version":"3.55.0"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2010CB529800"],"award-info":[{"award-number":["2010CB529800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2009CB918600"],"award-info":[{"award-number":["2009CB918600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National High Technology Research and Development Program of China","award":["2012AA020304"],"award-info":[{"award-number":["2012AA020304"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Sciences Foundation of China","doi-asserted-by":"crossref","award":["30530140"],"award-info":[{"award-number":["30530140"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Sciences Foundation of China","doi-asserted-by":"crossref","award":["31200557"],"award-info":[{"award-number":["31200557"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.toxicon.2013.05.022","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T15:17:19Z","timestamp":1370359039000},"page":"5-10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Cloning and characterization of a novel Kunitz-type inhibitor from scorpion with unique cysteine framework"],"prefix":"10.1016","volume":"72","author":[{"given":"Zongyun","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingliang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2013.05.022_bib1","doi-asserted-by":"crossref","first-page":"5059","DOI":"10.1182\/blood-2012-05-432005","article-title":"Identification of functionally important residues in TFPI Kunitz domain 3 required for the enhancement of its activity by protein S","volume":"120","author":"Ahnstrom","year":"2012","journal-title":"Blood"},{"key":"10.1016\/j.toxicon.2013.05.022_bib2","doi-asserted-by":"crossref","first-page":"23766","DOI":"10.1074\/jbc.C500064200","article-title":"Conkunitzin-S1 is the first member of a new Kunitz-type neurotoxin family. Structural and functional characterization","volume":"280","author":"Bayrhuber","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.05.022_bib3","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.jmb.2011.10.018","article-title":"Oxidative folding and structural analyses of a Kunitz-related inhibitor and its disulfide intermediates: functional implications","volume":"414","author":"Bronsoms","year":"2011","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib4","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.tree.2012.10.020","article-title":"Complex cocktails: the evolutionary novelty of venoms","volume":"28","author":"Casewell","year":"2012","journal-title":"Trends Ecol. Evol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib5","doi-asserted-by":"crossref","first-page":"e57529","DOI":"10.1371\/journal.pone.0057529","article-title":"SjAPI, the first functionally characterized Ascaris-type protease inhibitor from animal venoms","volume":"8","author":"Chen","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.toxicon.2013.05.022_bib6","doi-asserted-by":"crossref","first-page":"13813","DOI":"10.1074\/jbc.M112.343996","article-title":"Hg1, novel peptide inhibitor specific for Kv1.3 channels from first scorpion Kunitz-type potassium channel toxin family","volume":"287","author":"Chen","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.05.022_bib7","doi-asserted-by":"crossref","first-page":"e35154","DOI":"10.1371\/journal.pone.0035154","article-title":"Structural and functional diversity of acidic scorpion potassium channel toxins","volume":"7","author":"Chen","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.toxicon.2013.05.022_bib8","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.bbapap.2004.11.006","article-title":"Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization","volume":"1747","author":"Cheng","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2013.05.022_bib9","doi-asserted-by":"crossref","first-page":"e32269","DOI":"10.1371\/journal.pone.0032269","article-title":"Antifibrinolytic role of a bee venom serine protease inhibitor that acts as a plasmin inhibitor","volume":"7","author":"Choo","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.toxicon.2013.05.022_bib10","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/j.jmb.2004.09.088","article-title":"Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants","volume":"344","author":"Czapinska","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib11","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1160\/TH10-01-0073","article-title":"C1 inhibitor, a multi-functional serine protease inhibitor","volume":"104","author":"Davis","year":"2010","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.toxicon.2013.05.022_bib12","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.biochi.2011.08.003","article-title":"Identification and characterisation of Kunitz-type plasma kallikrein inhibitors unique to Oxyuranus sp. snake venoms","volume":"94","author":"Earl","year":"2012","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2013.05.022_bib13","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1016\/j.lfs.2004.10.053","article-title":"A serine proteinase inhibitor isolated from Tamarindus indica seeds and its effects on the release of human neutrophil elastase","volume":"76","author":"Fook","year":"2005","journal-title":"Life Sci."},{"key":"10.1016\/j.toxicon.2013.05.022_bib14","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.3390\/md10081812","article-title":"Sea anemone (Cnidaria, Anthozoa, Actiniaria) toxins: an overview","volume":"10","author":"Frazao","year":"2012","journal-title":"Marine Drugs"},{"key":"10.1016\/j.toxicon.2013.05.022_bib15","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1146\/annurev.genom.9.081307.164356","article-title":"The toxicogenomic multiverse: convergent recruitment of proteins into animal venoms","volume":"10","author":"Fry","year":"2009","journal-title":"Annu. Rev. Genomics Hum. Genet."},{"key":"10.1016\/j.toxicon.2013.05.022_bib16","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0041-0101(00)00162-8","article-title":"Twenty years of dendrotoxins","volume":"39","author":"Harvey","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2013.05.022_bib17","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1016\/j.peptides.2008.05.025","article-title":"Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom","volume":"29","author":"He","year":"2008","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2013.05.022_bib18","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.toxicon.2011.10.005","article-title":"Functional characterization of Kunitz-type protease inhibitor Pr-mulgins identified from New Guinean Pseudechis australis","volume":"59","author":"Inagaki","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2013.05.022_bib19","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1007\/s00239-012-9529-9","article-title":"Structural and molecular diversification of the Anguimorpha lizard mandibular venom gland system in the arboreal species Abronia graminea","volume":"75","author":"Koludarov","year":"2012","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib20","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.bi.49.070180.003113","article-title":"Protein inhibitors of proteinases","volume":"49","author":"Laskowski","year":"1980","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.toxicon.2013.05.022_bib21","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.peptides.2007.11.013","article-title":"A novel serine protease inhibitor from Bungarus fasciatus venom","volume":"29","author":"Lu","year":"2008","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2013.05.022_bib22","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1016\/j.jprot.2011.11.029","article-title":"Extreme diversity of scorpion venom peptides and proteins revealed by transcriptomic analysis: implication for proteome evolution of scorpion venom arsenal","volume":"75","author":"Ma","year":"2011","journal-title":"J. Proteomics"},{"key":"10.1016\/j.toxicon.2013.05.022_bib23","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.bcp.2011.03.023","article-title":"A bifunctional sea anemone peptide with Kunitz type protease and potassium channel inhibiting properties","volume":"82","author":"Peigneur","year":"2011","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib24","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"SignalP 4.0: discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.toxicon.2013.05.022_bib25","doi-asserted-by":"crossref","first-page":"1806","DOI":"10.1002\/pro.5560060902","article-title":"Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities","volume":"6","author":"Scheidig","year":"1997","journal-title":"Protein Sci."},{"key":"10.1016\/j.toxicon.2013.05.022_bib26","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1021\/bp0498793","article-title":"Role of arginine in protein refolding, solubilization, and purification","volume":"20","author":"Tsumoto","year":"2004","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.toxicon.2013.05.022_bib27","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1016\/S1357-2725(03)00134-1","article-title":"Serpins: structure, function and molecular evolution","volume":"35","author":"Van Gent","year":"2003","journal-title":"Int. J. Biochem. Cell. Biol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib28","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.cbpb.2009.02.010","article-title":"A novel serine protease inhibitor from the venom of Vespa bicolor Fabricius","volume":"153","author":"Yang","year":"2009","journal-title":"Comp. Biochem. Physiol. B., Biochem. Mol. Biol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib29","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1472-6807-11-3","article-title":"Differential molecular information of maurotoxin peptide recognizing IK(Ca) and Kv1.2 channels explored by computational simulation","volume":"11","author":"Yi","year":"2011","journal-title":"BMC Struct. Biol."},{"key":"10.1016\/j.toxicon.2013.05.022_bib30","doi-asserted-by":"crossref","first-page":"e3414","DOI":"10.1371\/journal.pone.0003414","article-title":"Discovery of a distinct superfamily of Kunitz-type toxin (KTT) from tarantulas","volume":"3","author":"Yuan","year":"2008","journal-title":"PloS One"},{"key":"10.1016\/j.toxicon.2013.05.022_bib31","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1073\/pnas.1109912109","article-title":"Nematode sperm maturation triggered by protease involves sperm-secreted serine protease inhibitor (Serpin)","volume":"109","author":"Zhao","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.toxicon.2013.05.022_bib32","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0014-5793(03)00693-8","article-title":"Adaptive evolution in the snake venom Kunitz\/BPTI protein family","volume":"547","author":"Zupunski","year":"2003","journal-title":"FEBS Lett."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010113002031?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010113002031?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T16:33:59Z","timestamp":1588955639000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010113002031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":32,"alternative-id":["S0041010113002031"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2013.05.022","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cloning and characterization of a novel Kunitz-type inhibitor from scorpion with unique cysteine framework","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2013.05.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}