{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:44:52Z","timestamp":1787960692018,"version":"build-2784847793"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Ministry of Higher Education, Scientific Research"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.toxicon.2013.06.017","type":"journal-article","created":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T13:26:07Z","timestamp":1372944367000},"page":"133-142","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["A thermoactive secreted phospholipase A2 purified from the venom glands of Scorpio maurus: Relation between the kinetic properties and the hemolytic activity"],"prefix":"10.1016","volume":"72","author":[{"given":"Hanen","family":"Louati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najeh","family":"Krayem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Fendri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imen","family":"Aissa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Sellami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofiane","family":"Bezzine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef","family":"Gargouri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2013.06.017_bib1","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.toxicon.2009.08.003","article-title":"Neurotoxic and cytotoxic effects of venom from different populations of the Egyptian Scorpio maurus palmatus","volume":"55","author":"Abdel-Rahman","year":"2010","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.toxicon.2008.12.007","article-title":"Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from different biotopes","volume":"53","author":"Abdel-Rahman","year":"2009","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib3","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/0041-0101(87)90136-X","article-title":"Determination of phospholipase A2 activity by a colorimetric assay using a pH indicator","volume":"25","author":"Araujo","year":"1987","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2013.06.017_bib4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib5","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0014-5793(99)01392-7","article-title":"Phospholipin, a novel heterodimeric phospholipase A2 from Pandinus imperator scp6pion venom","volume":"460","author":"Conde","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2013.06.017_bib6","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1016\/j.peptides.2008.05.021","article-title":"Myotoxic phospholipases A(2) isolated from Bothrops brazili snake venom and synthetic peptides derived from their C-terminal region: cytotoxic effect on microorganism and tumor cells","volume":"29","author":"Costa","year":"2008","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2013.06.017_bib7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0005-2736(75)90045-0","article-title":"Relation between various phospholipase actions on human red cell membranes and the interfacial phospholipid pressure in monolayers","volume":"406","author":"Demel","year":"1975","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2013.06.017_bib8","doi-asserted-by":"crossref","first-page":"6130","DOI":"10.1021\/cr200085w","article-title":"Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention","volume":"111","author":"Dennis","year":"2011","journal-title":"Chem. Rev."},{"key":"10.1016\/j.toxicon.2013.06.017_bib9","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.toxicon.2008.10.021","article-title":"Enzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima","volume":"53","author":"Diz Filho","year":"2009","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib10","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0041-0101(98)00125-1","article-title":"Structure-function properties of venom components from Australian elapids","volume":"37","author":"Fry","year":"1999","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib11","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.plipres.2009.08.002","article-title":"Amino acid-containing membrane lipids in bacteria","volume":"49","author":"Geiger","year":"2010","journal-title":"Prog. Lipid Res."},{"key":"10.1016\/j.toxicon.2013.06.017_bib12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1432-1033.1989.tb15172.x","article-title":"Purification and characterization of five variants of phospholipase A2 and complete primary structure of the main phospholipase A2 variant in Heloderma suspectum (Gila monster) venom","volume":"186","author":"Gomez","year":"1989","journal-title":"Eur. J. Biochem. FEBS"},{"key":"10.1016\/j.toxicon.2013.06.017_bib13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.toxicon.2012.09.006","article-title":"Phospholipases A2: unveiling the secrets of a functionally versatile group of snake venom toxins","volume":"62","author":"Guti\u00e9rrez","year":"2013","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib14","doi-asserted-by":"crossref","first-page":"32792","DOI":"10.1074\/jbc.272.52.32792","article-title":"Resistance to endotoxic shock in phospholipase A2 receptor-deficient mice","volume":"272","author":"Hanasaki","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib15","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1080\/10425170701243294","article-title":"Cloning, sequence analysis and homology modeling of a novel phospholipase A2 from Heterometrus fulvipes (Indian black scorpion)","volume":"18","author":"Hariprasad","year":"2007","journal-title":"DNA Seq. J. DNA Seq. Mapp."},{"key":"10.1016\/j.toxicon.2013.06.017_bib48","doi-asserted-by":"crossref","first-page":"7990","DOI":"10.1016\/S0021-9258(18)43377-7","article-title":"A gas-liquid solid phase peptide and protein sequenator","volume":"15","author":"Hewick","year":"1981","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib16","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.toxicon.2012.10.012","article-title":"Heteromtoxin (HmTx), a novel heterodimeric phospholipase A(2) from Heterometrus laoticus scorpion venom","volume":"61","author":"Incamnoi","year":"2013","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib17","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1093\/bja\/74.3.319","article-title":"Animal toxins","volume":"74","author":"Karalliedde","year":"1995","journal-title":"Br. J. Anaesth."},{"key":"10.1016\/j.toxicon.2013.06.017_bib18","doi-asserted-by":"crossref","first-page":"4545","DOI":"10.1111\/j.1742-4658.2009.07160.x","article-title":"Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2","volume":"276","author":"Karray","year":"2009","journal-title":"FEBS J."},{"key":"10.1016\/j.toxicon.2013.06.017_bib19","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0041-0101(87)90150-4","article-title":"The origin of snakes and evolution of the venom apparatus","volume":"25","author":"Kochva","year":"1987","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib20","doi-asserted-by":"crossref","first-page":"5768","DOI":"10.1016\/S0021-9258(18)83616-X","article-title":"Structure and properties of a human non-pancreatic phospholipase A2","volume":"264","author":"Kramer","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib21","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1432-1033.1989.tb15014.x","article-title":"Analysis of the cDNA for phospholipase A2 from honeybee venom glands. The deduced amino acid sequence reveals homology to the corresponding vertebrate enzymes","volume":"184","author":"Kuchler","year":"1989","journal-title":"Eur. J. Biochem. FEBS"},{"key":"10.1016\/j.toxicon.2013.06.017_bib22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2013.06.017_bib23","series-title":"Fauna Palaestina. Arachnida I: Scorpiones","author":"Levy","year":"1980"},{"key":"10.1016\/j.toxicon.2013.06.017_bib24","series-title":"A Full-length Protocol to Test Hemolytic Activity of Palytoxin on Human Erythrocytes","author":"Malagoli","year":"2007"},{"key":"10.1016\/j.toxicon.2013.06.017_bib25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.pep.2007.04.008","article-title":"Involvement of Gly 311 residue on substrate discrimination, pH and temperature dependency of recombinant Staphylococcus xylosus lipase: a study with emulsified substrate","volume":"55","author":"Mosbah","year":"2007","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2013.06.017_bib26","doi-asserted-by":"crossref","first-page":"24987","DOI":"10.1074\/jbc.M502088200","article-title":"Cellular distribution, post-translational modification, and tumorigenic potential of human group III secreted phospholipase A(2)","volume":"280","author":"Murakami","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib27","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/j.toxicon.2003.11.014","article-title":"A structure based model for liposome disruption and the role of catalytic activity in myotoxic phospholipase A2s","volume":"42","author":"Murakami","year":"2003","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib28","doi-asserted-by":"crossref","first-page":"619","DOI":"10.5551\/jat.2857","article-title":"Smoking-induced alterations in platelet membrane fluidity and Na(+)\/K(+)-ATPase activity in chronic cigarette smokers","volume":"17","author":"Padmavathi","year":"2010","journal-title":"J. Atheroscler. Thromb."},{"key":"10.1016\/j.toxicon.2013.06.017_bib29","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.1021\/bi00580a001","article-title":"Regulation of phospholipase A2 activity by the lipid-water interface: a monolayer approach","volume":"18","author":"Pattus","year":"1979","journal-title":"Biochemistry (Mosc.)"},{"key":"10.1016\/j.toxicon.2013.06.017_bib30","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1111\/j.1432-1033.1983.tb07411.x","article-title":"Hydrolysis of mixed monomolecular films of phosphatidylcholine\/triacylglycerol by pancreatic phospholipase A2","volume":"132","author":"Pieroni","year":"1983","journal-title":"Eur. J. Biochem. Febs"},{"key":"10.1016\/j.toxicon.2013.06.017_bib31","first-page":"931","article-title":"Purification and properties of phospholipase A2 from the venom of scorpion, (Heterometrus fulvipes)","volume":"20","author":"Ramanaiah","year":"1990","journal-title":"Biochem. Int."},{"key":"10.1016\/j.toxicon.2013.06.017_bib32","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/S0167-4781(03)00143-X","article-title":"Molecular characterization of a gene encoding the Drosophila melanogaster phospholipase A2","volume":"1628","author":"Ryu","year":"2003","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2013.06.017_bib33","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.bbalip.2006.07.011","article-title":"The phospholipase A2 superfamily and its group numbering system","volume":"1761","author":"Schaloske","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2013.06.017_bib34","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1126\/science.2274785","article-title":"Interfacial catalysis: the mechanism of phospholipase A2","volume":"250","author":"Scott","year":"1990","journal-title":"Science"},{"key":"10.1016\/j.toxicon.2013.06.017_bib35","doi-asserted-by":"crossref","first-page":"48535","DOI":"10.1074\/jbc.M205855200","article-title":"Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2","volume":"277","author":"Singer","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1388-1981(00)00105-0","article-title":"The expanding superfamily of phospholipase A(2) enzymes: classification and characterization","volume":"1488","author":"Six","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2013.06.017_bib37","doi-asserted-by":"crossref","first-page":"739","DOI":"10.2174\/1566524013363258","article-title":"Mammalian secreted phospholipases A2 and their pathophysiological significance in inflammatory diseases","volume":"1","author":"Touqui","year":"2001","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.toxicon.2013.06.017_bib38","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/S0041-0101(03)00085-0","article-title":"Structural, enzymatic and biological properties of new PLA(2) isoform from Crotalus durissus terrificus venom","volume":"41","author":"Toyama","year":"2003","journal-title":"Toxicon Off. J. Int. Soc. Toxinology"},{"key":"10.1016\/j.toxicon.2013.06.017_bib39","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1111\/j.1432-1033.2004.04047.x","article-title":"Phaiodactylipin, a glycosylated heterodimeric phospholipase A from the venom of the scorpion Anuroctonus phaiodactylus","volume":"271","author":"Valdez-Cruz","year":"2004","journal-title":"Eur. J. Biochem. FEBS"},{"key":"10.1016\/j.toxicon.2013.06.017_bib40","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.gene.2007.03.007","article-title":"Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus","volume":"396","author":"Valdez-Cruz","year":"2007","journal-title":"Gene"},{"key":"10.1016\/j.toxicon.2013.06.017_bib41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S1388-1981(00)00110-4","article-title":"Increasing molecular diversity of secreted phospholipases A(2) and their receptors and binding proteins","volume":"1488","author":"Valentin","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2013.06.017_bib42","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0009-3084(73)90009-1","article-title":"Enzyme reactions in a membrane model. 1. A new technique to study enzyme reactions in monolayers","volume":"10","author":"Verger","year":"1973","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.toxicon.2013.06.017_bib43","series-title":"Lipase","first-page":"121","article-title":"Pancreatic lipases","author":"Verger","year":"1984"},{"key":"10.1016\/j.toxicon.2013.06.017_bib44","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1432-1033.1980.tb04982.x","article-title":"Correlation of enzymatic activity and anticoagulant properties of phospholipase A2","volume":"112","author":"Verheij","year":"1980","journal-title":"Eur. J. Biochem. FEBS"},{"key":"10.1016\/j.toxicon.2013.06.017_bib45","doi-asserted-by":"crossref","first-page":"11886","DOI":"10.1074\/jbc.272.18.11886","article-title":"The mechanism of inhibition of ryanodine receptor channels by Imperatoxin I, a heterodimeric protein from the scorpion Pandinus imperator","volume":"272","author":"Zamudio","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.06.017_bib46","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.abb.2009.11.020","article-title":"A novel hepatopancreatic phospholipase A2 from Hexaplex trunculus with digestive and toxic activities","volume":"494","author":"Zarai","year":"2010","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2013.06.017_bib47","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.toxicon.2009.01.003","article-title":"Two purified and characterized phospholipases A2 from Cerastes cerastes venom, that inhibit cancerous cell adhesion and migration","volume":"53","author":"Zouari-Kessentini","year":"2009","journal-title":"Toxicon"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010113002377?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010113002377?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T21:08:40Z","timestamp":1758575320000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010113002377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":48,"alternative-id":["S0041010113002377"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2013.06.017","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A thermoactive secreted phospholipase A2 purified from the venom glands of Scorpio maurus: Relation between the kinetic properties and the hemolytic activity","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2013.06.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}