{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:21:47Z","timestamp":1767374507482,"version":"3.40.4"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2014,3]]},"DOI":"10.1016\/j.toxicon.2013.12.002","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T04:45:17Z","timestamp":1389156317000},"page":"64-71","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Enzyme specificity and effects of gyroxin, a serine protease from the venom of the South American rattlesnake Crotalus durissus terrificus, on protease-activated receptors"],"prefix":"10.1016","volume":"79","author":[{"given":"Camila M.","family":"Yonamine","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcia Y.","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela B.","family":"Nering","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iuri E.","family":"Gouv\u00eaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Alberto A.","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro R.B.","family":"Prieto da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Yamane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A.","family":"Juliano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Juliano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f4nio J.","family":"Lapa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirian A.F.","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa R.","family":"Lima-Landman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2013.12.002_bib1","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1124\/jpet.102.043844","article-title":"Selective tryptic cleavage at the tethered ligand site of the amino terminal domain of proteinase-activated receptor-2 in intact cells","volume":"304","author":"Al-Ani","year":"2003","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.toxicon.2013.12.002_bib2","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/0041-0101(88)90260-7","article-title":"Gyroxin, a toxin from the venom of Crotalus durissus terrificus, is a thrombin-like enzyme","volume":"26","author":"Alexander","year":"1988","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2013.12.002_bib3","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s10967-006-0268-5","article-title":"Biodistribution of gyroxin using 125I as radiotracer","volume":"269","author":"Alves da Silva","year":"2006","journal-title":"J. Radioanal. Nucl. Chem."},{"key":"10.1016\/j.toxicon.2013.12.002_bib4","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.toxicon.2010.06.027","article-title":"Gyroxin increases blood-brain barrier permeability to Evans blue dye in mice","volume":"57","author":"Alves da Silva","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2013.12.002_bib5","first-page":"224","article-title":"Gyroxin, a new neurotoxin of Crotalus durissus terrificus venom","volume":"11","author":"Barrio","year":"1961","journal-title":"Acta Physiol. Latinoam."},{"key":"10.1016\/j.toxicon.2013.12.002_bib6","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem"},{"key":"10.1016\/j.toxicon.2013.12.002_bib7","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/S0041-0101(00)00222-1","article-title":"Gyroxin fails to modify in\u00a0vitro release of labelled dopamine and acetylcholine from rat and mouse striatal tissue","volume":"39","author":"Camillo","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2013.12.002_bib8","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/0006-8993(75)90586-7","article-title":"Barrel rotation induced by somatostatin in the non-lesioned rat","volume":"96","author":"Cohn","year":"1975","journal-title":"Brain Res."},{"key":"10.1016\/j.toxicon.2013.12.002_bib9","doi-asserted-by":"crossref","first-page":"11023","DOI":"10.1073\/pnas.96.20.11023","article-title":"How the protease thrombin talks to cells","volume":"96","author":"Coughlin","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.toxicon.2013.12.002_bib10","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1038\/35025229","article-title":"Thrombin signalling and protease-activated receptors","volume":"407","author":"Coughlin","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2013.12.002_bib11","doi-asserted-by":"crossref","first-page":"281","DOI":"10.2174\/092986712803414123","article-title":"Gyroxin and its biological activity: effects on CNS basement membranes and endothelium and protease-activated receptors","volume":"19","author":"Da Silva","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.toxicon.2013.12.002_bib12","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1016\/S0142-9612(01)00012-6","article-title":"Study of a (trimethylenecarbonate-co-epsiloncaprolactone) polymer\u2013part 2: in\u00a0vitro cytocompatibility analysis and in\u00a0vivo ED1 cell response of a new nerve guide","volume":"22","author":"Fabre","year":"2001","journal-title":"Biomaterials"},{"key":"10.1016\/j.toxicon.2013.12.002_bib13","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0022-1759(94)00277-4","article-title":"Microculture tetrazolium assays: a comparison between two new tetrazolium salts, XTT and MTS","volume":"179","author":"Goodwin","year":"1995","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.toxicon.2013.12.002_bib14","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0076-6879(85)09097-8","article-title":"Primary glial cultures as a model for studying hormone action","volume":"109","author":"Hamprecht","year":"1985","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.toxicon.2013.12.002_bib15","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/BF00119771","article-title":"Internally quenched fluorogenic protease substrates: solidphase synthesis and fluorescence spectroscopy of peptides containing orthoaminobenzoyl\/dinitrophenyl groups as donor-acceptor pairs","volume":"6","author":"Hirata","year":"1995","journal-title":"Lett. Pept. Sci."},{"key":"10.1016\/j.toxicon.2013.12.002_bib16","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/29325","article-title":"A dual thrombin receptor system for platelet activation","volume":"394","author":"Kahn","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2013.12.002_bib17","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nat. Lond."},{"issue":"28","key":"10.1016\/j.toxicon.2013.12.002_bib18","doi-asserted-by":"crossref","first-page":"21487","DOI":"10.1074\/jbc.M109.092692","article-title":"Induced-fit mechanism for prolyl endopeptidase","volume":"285","author":"Li","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2013.12.002_bib19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0031-6997(24)01493-5","article-title":"Proteinase activated receptors","volume":"53","author":"Macfarlane","year":"2001","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.toxicon.2013.12.002_bib20","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.bcp.2007.09.002","article-title":"Characterization of a new peptide agonist of the protease-activated receptor-1","volume":"75","author":"Mao","year":"2008","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.toxicon.2013.12.002_bib21","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/abio.2001.5115","article-title":"Synthesis and hydrolysis by cysteine and serine proteases of short internally quenched fluorogenic peptides","volume":"293","author":"Melo","year":"2001","journal-title":"Anal. Biochem"},{"key":"10.1016\/j.toxicon.2013.12.002_bib22","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1038\/35007085","article-title":"PAR3 is a cofactor for PAR4 activation by thrombin","volume":"404","author":"Nakanishi-Matsui","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2013.12.002_bib23","doi-asserted-by":"crossref","first-page":"796","DOI":"10.2174\/092986610791190363","article-title":"Salt effect on substrate specificity of a subtilisin-like halophilic protease","volume":"17","author":"Okamoto","year":"2010","journal-title":"Protein Pept. Lett."},{"issue":"73","key":"10.1016\/j.toxicon.2013.12.002_bib24","first-page":"425","article-title":"Developing PAR1 antagonists: minding the endothelial gap","volume":"13","author":"Ramachandran","year":"2012","journal-title":"Discov. Med."},{"key":"10.1016\/j.toxicon.2013.12.002_bib25","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1006\/abio.1998.3001","article-title":"Lipid analysis by matrix-assisted laser desorption and ionization mass spectrometry: a methodological approach","volume":"267","author":"Schiller","year":"1999","journal-title":"Anal. Biochem"},{"key":"10.1016\/j.toxicon.2013.12.002_bib26","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s12264-010-0813-7","article-title":"Excitotoxicity effects of glutamate on human neuroblastoma SH-SY5Y cells via oxidative damage","volume":"26","author":"Sun","year":"2010","journal-title":"Neurosci. Bull."},{"key":"10.1016\/j.toxicon.2013.12.002_bib27","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/(SICI)1098-1136(199712)21:4<361::AID-GLIA3>3.0.CO;2-6","article-title":"Characteristics of thrombin-induced calcium signals in rat astrocytes","volume":"21","author":"Ubl","year":"1997","journal-title":"Glia"},{"key":"10.1016\/j.toxicon.2013.12.002_bib28","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1002\/glia.10012","article-title":"Four subtypes of protease-activated receptors, co-expressed in rat astrocytes, evoke different physiological signaling","volume":"37","author":"Wang","year":"2002","journal-title":"Glia"},{"key":"10.1016\/j.toxicon.2013.12.002_bib29","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1016\/j.biochi.2012.07.020","article-title":"Kinetic characterization of gyroxin, a serine protease from Crotalus durissus terrificus venom","volume":"94","author":"Yonamine","year":"2012","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2013.12.002_bib30","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.toxicon.2009.03.022","article-title":"Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional Gyroxin homologue in COS-7 cells","volume":"54","author":"Yonamine","year":"2009","journal-title":"Toxicon"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010113004583?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010113004583?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T11:40:40Z","timestamp":1746099640000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010113004583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3]]},"references-count":30,"alternative-id":["S0041010113004583"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2013.12.002","relation":{},"ISSN":["0041-0101"],"issn-type":[{"type":"print","value":"0041-0101"}],"subject":[],"published":{"date-parts":[[2014,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Enzyme specificity and effects of gyroxin, a serine protease from the venom of the South American rattlesnake Crotalus durissus terrificus, on protease-activated receptors","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2013.12.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}