{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:17:22Z","timestamp":1777501042070,"version":"3.51.4"},"reference-count":41,"publisher":"Elsevier BV","content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2014,6]]},"DOI":"10.1016\/j.toxicon.2014.04.002","type":"journal-article","created":{"date-parts":[[2014,4,12]],"date-time":"2014-04-12T22:01:18Z","timestamp":1397340078000},"page":"56-64","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Whole venom of Loxosceles similis activates caspases-3, -6, -7, and -9 in human primary skin fibroblasts"],"prefix":"10.1016","volume":"84","author":[{"given":"Arthur Estanislau","family":"Dantas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Campolina Rebello","family":"Horta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais M.M.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson Oliveira","family":"do Carmo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara Bruna Ribeiro de Oliveira","family":"Mendes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo M.","family":"Goes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evanguedes","family":"Kalapothakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-991X","authenticated-orcid":false,"given":"Dawidson A.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.toxicon.2014.04.002_bib3","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.toxicon.2004.12.009","article-title":"Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas","volume":"45","author":"Barbaro","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2014.04.002_bib29","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem"},{"key":"10.1016\/j.toxicon.2014.04.002_bib4","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.taap.2005.05.015","article-title":"Brown spider dermonecrotic toxin directly induces nephrotoxicity","volume":"211","author":"Chaim","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"1","key":"10.1016\/j.toxicon.2014.04.002_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxicon.2012.03.007","article-title":"Cutaneous loxoscelism caused by Loxosceles similis venom and neutralization capacity of its specific antivenom","volume":"60","author":"Chatzaki","year":"2012","journal-title":"Toxicon: Off. J. Int. Soc. Toxinol."},{"issue":"4","key":"10.1016\/j.toxicon.2014.04.002_bib6","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1002\/jcb.22148","article-title":"Identification of a direct hemolytic effect dependent on the catalytic activity induced by phospholipase-d (dermonecrotic toxin) from brown spider venom","volume":"107","author":"Chaves-Moreira","year":"2009","journal-title":"J. Cell. Biochem."},{"issue":"9","key":"10.1016\/j.toxicon.2014.04.002_bib7","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1002\/jcb.23177","article-title":"The relationship between calcium and the metabolism of plasma membrane phospholipids in hemolysis induced by brown spider venom phospholipase-d toxin","volume":"112","author":"Chaves-Moreira","year":"2011","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.toxicon.2014.04.002_bib30","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/A:1023470829671","article-title":"Purification and characterization of Loxnecrogin, a dermonecrotic toxin from Loxosceles gaucho brown spider venom","volume":"22","author":"Cunha","year":"2003","journal-title":"J. Prot. Chem."},{"issue":"7","key":"10.1016\/j.toxicon.2014.04.002_bib8","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.toxicon.2004.07.012","article-title":"Brown spiders and loxoscelism","volume":"44","author":"Da Silva","year":"2004","journal-title":"Toxicon: Off. J. Int. Soc. Toxinol."},{"key":"10.1016\/j.toxicon.2014.04.002_bib9","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0041-0101(02)00078-8","article-title":"Identification of proteases in the extract of venom glands from brown spiders","volume":"40","author":"Da Silveira","year":"2002","journal-title":"Toxicon"},{"issue":"6","key":"10.1016\/j.toxicon.2014.04.002_bib31","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.toxicon.2006.11.024","article-title":"Hyaluronidases in Loxosceles intermedia (Brown spider) venom are endo-beta-N-acetyl-d-hexosaminidases hydrolases","volume":"49","author":"da Silveira","year":"2007","journal-title":"Toxicon"},{"issue":"2","key":"10.1016\/j.toxicon.2014.04.002_bib32","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1042\/BJ20070363","article-title":"Identification, cloning, expression and functional characterization of an astacin-like metalloprotease toxin from Loxosceles intermedia (brown spider) venom","volume":"406","author":"da Silveira","year":"2007","journal-title":"Biochem J"},{"issue":"6","key":"10.1016\/j.toxicon.2014.04.002_bib33","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.toxicon.2006.11.024","article-title":"Hyaluronidases in Loxosceles intermedia (Brown spider) venom are endo-beta-N-acetyl-d-hexosaminidases hydrolases","volume":"49","author":"da Silveira","year":"2007","journal-title":"Toxicon"},{"issue":"8","key":"10.1016\/j.toxicon.2014.04.002_bib10","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1016\/j.toxicon.2010.08.008","article-title":"Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1","volume":"56","author":"Dias-Lopes","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2014.04.002_bib34","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0041-0101(97)00083-4","article-title":"Detection and characterization of metal- loproteinases with gelatinolytic, fibronectinolytic and fibrinogeno- lytic activities in brown spider (Loxosceles intermedia) venom","volume":"36","author":"Feitosa","year":"1998","journal-title":"Toxicon"},{"issue":"5","key":"10.1016\/j.toxicon.2014.04.002_bib11","doi-asserted-by":"crossref","first-page":"e2206","DOI":"10.1371\/journal.pntd.0002206","article-title":"A novel hyaluronidase from brown spider (Loxosceles intermedia) venom (Dietrich's Hyaluronidase): from cloning to functional characterization","volume":"7","author":"Ferrer","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"4","key":"10.1016\/j.toxicon.2014.04.002_bib35","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/00000441-199210000-00008","article-title":"Loxoscelism","volume":"304","author":"Futrell","year":"1992","journal-title":"Am. J. Med. Sci."},{"issue":"1","key":"10.1016\/j.toxicon.2014.04.002_bib12","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/cdd.2011.96","article-title":"Molecular definitions of cell death subroutines: recommendations of the nomenclature committee on cell death 2012","volume":"19","author":"Galluzzi","year":"2012","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.toxicon.2014.04.002_bib36","first-page":"117","article-title":"The spider genus Loxosceles in South America (Araneae, Scytodidae)","volume":"136","author":"Gertsch","year":"1967","journal-title":"Bull. Am. Museum Nat. Hist."},{"key":"10.1016\/j.toxicon.2014.04.002_bib37","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0962-8924(98)01273-2","article-title":"The central executioners of apoptosis: caspases or mitochondria?","volume":"8","author":"Green","year":"1998","journal-title":"Trends Cell Biol"},{"key":"10.1016\/j.toxicon.2014.04.002_bib13","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.toxicon.2013.04.018","article-title":"Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: neutralization of its toxic effects by anti-loxoscelic antivenoms","volume":"70","author":"Guimar\u00e3es","year":"2013","journal-title":"Toxicon: Off. J. Int. Soc. Toxinol."},{"issue":"3","key":"10.1016\/j.toxicon.2014.04.002_bib38","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1083\/jcb.200310015","article-title":"Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and Abeta-induced cell death","volume":"165","author":"Hitomi","year":"2004","journal-title":"J. Cell Biol."},{"issue":"6","key":"10.1016\/j.toxicon.2014.04.002_bib14","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1038\/jid.2013.40","article-title":"Lysophosphatidic acid mediates the release of cytokines and chemokines by human fibroblasts treated with loxosceles spider venom","volume":"133","author":"Horta","year":"2013","journal-title":"J. Investig. Dermatol."},{"key":"10.1016\/j.toxicon.2014.04.002_bib39","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/bjc.1972.33","article-title":"Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics","volume":"26","author":"Kerr","year":"1972","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.toxicon.2014.04.002_bib40","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1002\/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-J","article-title":"Apoptosis: its significance in cancer and cancer therapy","volume":"73","author":"Kerr","year":"1994","journal-title":"Cancer"},{"key":"10.1016\/j.toxicon.2014.04.002_bib41","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/cdd.2008.150","article-title":"Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009","volume":"16","author":"Kroemer","year":"2009","journal-title":"Cell Death Differ."},{"issue":"9","key":"10.1016\/j.toxicon.2014.04.002_bib15","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1038\/cdd.2008.91","article-title":"Cell death in the host response to infection","volume":"15","author":"Labb\u00e9","year":"2008","journal-title":"Cell Death Differ."},{"issue":"2","key":"10.1016\/j.toxicon.2014.04.002_bib16","doi-asserted-by":"crossref","first-page":"113","DOI":"10.35699\/2675-5327.2005.22104","article-title":"Sobre a presen\u00e7a de tr\u00eas esp\u00e9cies de Loxosceles Heineken & Lowe (Araneae: Sicariidae) no munic\u00edpio de Belo Horizonte, Minas Gerais, Brasil","volume":"6","author":"Machado","year":"2005","journal-title":"Lundiana"},{"key":"10.1016\/j.toxicon.2014.04.002_bib42","series-title":"Sistema Nacional de Notifica\u00e7\u00e3o de Agravos","author":"Minist\u00e9rio da Sa\u00fade","year":"2011"},{"issue":"1\u20132","key":"10.1016\/j.toxicon.2014.04.002_bib17","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"issue":"4","key":"10.1016\/j.toxicon.2014.04.002_bib18","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.toxicon.2010.03.027","article-title":"Brown spider venom toxins interact with cell surface and are endocytosed by rabbit endothelial cells","volume":"56","author":"Nowatzki","year":"2010","journal-title":"Toxicon: Off. J. Int. Soc. Toxinol."},{"issue":"3","key":"10.1016\/j.toxicon.2014.04.002_bib19","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.toxicon.2012.04.333","article-title":"Brown spider (Loxosceles intermedia) venom triggers endothelial cells death by anoikis","volume":"60","author":"Nowatzki","year":"2012","journal-title":"Toxicon: Off. J. Int. Soc. Toxinol."},{"issue":"1","key":"10.1016\/j.toxicon.2014.04.002_bib20","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/sj.jid.5700049","article-title":"Role of matrix metalloproteinases in HaCaT keratinocytes apoptosis induced by Loxosceles venom sphingomyelinase D","volume":"126","author":"Paix\u00e3o-Cavalcante","year":"2006","journal-title":"J. Investig. Dermatol."},{"issue":"6","key":"10.1016\/j.toxicon.2014.04.002_bib21","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1038\/sj.jid.5700688","article-title":"Tetracycline protects against dermonecrosis induced by loxosceles spider venom","volume":"127","author":"Paix\u00e3o-Cavalcante","year":"2007","journal-title":"J. Investig. Dermatol."},{"key":"10.1016\/j.toxicon.2014.04.002_bib22","first-page":"1","article-title":"Establishing primary adult fibroblast cultures from rodents","author":"Seluanov","year":"2010","journal-title":"J. Vis. Exp. JoVE"},{"issue":"4","key":"10.1016\/j.toxicon.2014.04.002_bib23","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.4049\/jimmunol.1101620","article-title":"Caspase-4 is required for activation of inflammasomes","volume":"188","author":"Sollberger","year":"2012","journal-title":"J. Immunol. (Baltimore, Md.: 1950)"},{"issue":"1","key":"10.1016\/j.toxicon.2014.04.002_bib43","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1046\/j.1365-2567.2002.01483.x","article-title":"Mechanismofinductionofcomplementsusceptibilityof erythrocytesby spider and bacterial sphingomyelinases","volume":"107","author":"Tambourgi","year":"2002","journal-title":"Immunology"},{"issue":"3","key":"10.1016\/j.toxicon.2014.04.002_bib24","first-page":"231","article-title":"Apoptosis: controlled demolition at the cellular level. Nature reviews","volume":"9","author":"Taylor","year":"2008","journal-title":"Mol. Cell. Biol."},{"issue":"6","key":"10.1016\/j.toxicon.2014.04.002_bib25","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1002\/cbf.1774","article-title":"Apoptosis: why and how does it occur in biology?","volume":"29","author":"Ulukaya","year":"2011","journal-title":"Cell Biochem. Funct."},{"issue":"7","key":"10.1016\/j.toxicon.2014.04.002_bib26","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1023\/A:1004031019827","article-title":"Effect of brown spider venom on basement membrane structures","volume":"32","author":"Veiga","year":"2000","journal-title":"Histochem. J."},{"key":"10.1016\/j.toxicon.2014.04.002_bib27","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1590\/S0100-879X2001000700002","article-title":"Extracellular matrix molecules as targets for brown spider venom toxins","volume":"34","author":"Veiga","year":"2001","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.toxicon.2014.04.002_bib28","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.toxicon.2013.01.027","article-title":"Modulation of membrane phospholipids, the cytosolic calcium influx and cell proliferation following treatment of B16-F10 cells with recombinant phospholipase-D from Loxosceles intermedia (brown spider) venom","volume":"67","author":"Wille","year":"2013","journal-title":"Toxicon"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010114001032?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010114001032?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T02:40:14Z","timestamp":1689129614000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010114001032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":41,"alternative-id":["S0041010114001032"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2014.04.002","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2014,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Whole venom of Loxosceles similis activates caspases-3, -6, -7, and -9 in human primary skin fibroblasts","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2014.04.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}