{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T11:08:02Z","timestamp":1781953682440,"version":"3.54.5"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"S\u00e3o Paulo State Research Foundation","award":["#2012\/10993-4"],"award-info":[{"award-number":["#2012\/10993-4"]}]},{"name":"S\u00e3o Paulo State Research Foundation","award":["#2013\/00798-2"],"award-info":[{"award-number":["#2013\/00798-2"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2016,2]]},"DOI":"10.1016\/j.toxicon.2015.11.012","type":"journal-article","created":{"date-parts":[[2015,11,22]],"date-time":"2015-11-22T14:47:11Z","timestamp":1448203631000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Alternagin-C (ALT-C), a disintegrin-like protein from Rhinocerophis alternatus snake venom promotes positive inotropism and chronotropism in fish heart"],"prefix":"10.1016","volume":"110","author":[{"given":"D.A.","family":"Monteiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.L.","family":"Kalinin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.S.","family":"Selistre-de-Araujo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.S.","family":"Vasconcelos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.T.","family":"Rantin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2015.11.012_bib1","first-page":"36","article-title":"Image processing with image","volume":"11","author":"Abramoff","year":"2004","journal-title":"J.\u00a0Biophot. Int."},{"key":"10.1016\/j.toxicon.2015.11.012_bib2","series-title":"Analytical Techniques. Biochemistry and Molecular Biology of Fishes","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/B978-0-444-82033-4.50008-8","article-title":"Cannulation techniques","author":"Axelsson","year":"1994"},{"key":"10.1016\/j.toxicon.2015.11.012_bib3","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/2044-5040-1-32","article-title":"Transgenic overexpression of \u03b3-cytoplasmic actin protects against eccentric contraction-induced force loss in mdx mice","volume":"1","author":"Baltgalvis","year":"2011","journal-title":"Skelet. Muscle"},{"key":"10.1016\/j.toxicon.2015.11.012_bib4","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1113\/jphysiol.1994.sp020130","article-title":"Relaxation in rabbit and rat cardiac cells: species-dependent differences in cellular mechanisms","author":"Bassani","year":"1994","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib5","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1161\/01.RES.73.5.813","article-title":"Effects of thapsigargin and cyclopiazonic acid on twitch force and sarcoplasmic reticulum Ca2+ content of rabbit ventricular muscle","volume":"73","author":"Baudet","year":"1993","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib6","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.bbamcr.2008.10.005","article-title":"Inositol trisphosphate and calcium signalling mechanisms","volume":"1793","author":"Berridge","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2015.11.012_bib7","first-page":"H366","article-title":"Ca influx and sarcoplasmic reticulum Ca release in cardiac muscle activation during post-rest recovery","volume":"248","author":"Bers","year":"1985","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib8","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1590\/S0066-782X2012005000016","article-title":"Myocardial remodeling after large infarcts in rat converts post rest-potentiation in force decay","volume":"98","author":"Bocalini","year":"2012","journal-title":"Arq. Bras. Cardiol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib9","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1161\/01.RES.0000164359.95588.25","article-title":"Targeting protein phosphatase 1 in heart failure","volume":"96","author":"Champion","year":"2005","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib10","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1042\/bj3010879","article-title":"Quantal Ca2+ mobilization by ryanodine receptors is due to all-or-none release from functionally discrete intracellular stores","volume":"301","author":"Cheek","year":"1994","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxicon.2015.11.012_bib11","doi-asserted-by":"crossref","first-page":"18247","DOI":"10.1074\/jbc.M311771200","article-title":"Alternagin-C, a disintegrin-like protein, induces vascular endothelial cell growth factor (VEGF) expression and endothelial cell proliferation in\u00a0vitro","volume":"279","author":"Cominetti","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2015.11.012_bib12","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1093\/cvr\/cvp416","article-title":"FAK mediates the activation of cardiac fibroblasts induced by mechanical stress through regulation of the mTOR complex","volume":"86","author":"Dalla Costa","year":"2010","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib13","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jprot.2009.01.015","article-title":"Timeline of key events in snake venom metalloproteinase research","volume":"72","author":"Fox","year":"2009","journal-title":"J.\u00a0Proteomics"},{"key":"10.1016\/j.toxicon.2015.11.012_bib14","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1161\/01.RES.87.7.558","article-title":"Early activation of the multicomponent signaling complex associated with focal adhesion kinase induced by pressure overload in the rat heart","volume":"87","author":"Franchini","year":"2000","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib15","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1469-7793.1998.231bx.x","article-title":"Mechanisms of force inhibition by halothane and isoflurane in intact rat cardiac muscle","volume":"506","author":"Hanley","year":"1998","journal-title":"J.\u00a0Physiol. (Lond.)"},{"key":"10.1016\/j.toxicon.2015.11.012_bib16","series-title":"Mechanics and Energetics of the Myocardium","author":"Holubarsch","year":"2002"},{"key":"10.1016\/j.toxicon.2015.11.012_bib17","doi-asserted-by":"crossref","first-page":"13609","DOI":"10.1074\/jbc.271.23.13609","article-title":"Phosphorylation-dependent regulation of cardiac Na+\/Ca2+ exchanger via protein kinase C","volume":"271","author":"Iwamoto","year":"1996","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2015.11.012_bib18","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1590\/S0100-879X1998000700001","article-title":"Snake venom metalloproteinases and disintegrins: interactions with cells","volume":"31","author":"Kamiguti","year":"1998","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib19","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1161\/01.RES.87.12.1095","article-title":"Regulation of cardiac L-type calcium channels by protein kinase A and protein kinase C","volume":"87","author":"Kamp","year":"2000","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib20","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3389\/fnmol.2011.00051","article-title":"PI3K\/AKT\/mTOR pathway in angiogenesis","volume":"4","author":"Karar","year":"2011","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.toxicon.2015.11.012_bib21","series-title":"Methods in Molecular Biology. Basic Protein and Peptide Protocols","first-page":"9","article-title":"The Bradford method for protein quantitation","author":"Kruger","year":"1994"},{"key":"10.1016\/j.toxicon.2015.11.012_bib22","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1242\/jeb.198.12.2491","article-title":"The length dependence of work production in rat papillary-muscles in-vitro","volume":"198","author":"Layland","year":"1995","journal-title":"J.\u00a0Exp. Biol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib23","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1139\/y95-081","article-title":"Characteristics of tetanic contractions in caffeinetreated rat myocardium","volume":"73","author":"Leite","year":"1995","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib24","doi-asserted-by":"crossref","first-page":"856","DOI":"10.2310\/JIM.0b013e3181c5e61f","article-title":"Integrins, focal adhesions and cardiac fibroblasts","volume":"57","author":"Manso","year":"2009","journal-title":"J.\u00a0Investig. Med."},{"key":"10.1016\/j.toxicon.2015.11.012_bib25","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1242\/jeb.167.1.203","article-title":"Effect of season and temperature-acclimation on the function of crucian carp (Carassius carassius) heart","volume":"167","author":"Matikainen","year":"1992","journal-title":"J.\u00a0Exp. Biol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib26","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1590\/S1678-91992009000200013","article-title":"ALT-C, a disintegrin-like cys-rich protein from Bothrops alternatus, increases skeletal myoblast viability","volume":"15","author":"Mesquita-Ferrari","year":"2009","journal-title":"J.\u00a0Venom. Anim. Tox. Incl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2015.11.012_bib27","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1139\/y98-013","article-title":"Comparison of the contractile performance of the hypertrophied myocardium from spontaneous hypertensive rats and normotensive infarcted rats","volume":"76","author":"Mill","year":"1998","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib28","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/s00360-008-0331-3","article-title":"The positive force-frequency relationship is maintained in absence of sarcoplasmic reticulum function in rabbit, but not in rat myocardium","volume":"179B","author":"Monasky","year":"2009","journal-title":"J.\u00a0Comp. Physiol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1152\/physiolgenomics.00107.2002","article-title":"Abnormal contractile activity and calcium cycling in cardiac myocytes isolated from dmpk knockout mice","volume":"13","author":"Pall","year":"2003","journal-title":"Physiol. Genomics"},{"key":"10.1016\/j.toxicon.2015.11.012_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2007.02.021","article-title":"Modulation of in\u00a0vitro and in\u00a0vivo angiogenesis by alternagin-C, a disintegrin-like protein from Bothrops alternatus snake venom and by a peptide derived from its sequence","volume":"461","author":"Ramos","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2015.11.012_bib31","first-page":"328","article-title":"Snake venom metalloproteases \u2013 structure and function of catalytic and disintegrin domains","volume":"142C","author":"Ramos","year":"2006","journal-title":"Comp. Biochem. Physiol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib32","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1600-0773.1997.tb00038.x","article-title":"Mechanical restitution in atrial muscle from human and rat hearts: effects of agents that modify sarcoplasmic reticulum function","volume":"81","author":"Ravens","year":"1997","journal-title":"Pharmacol. Toxicol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib33","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.cardiores.2004.04.027","article-title":"Molecular and mechanical synergy: cross-talk between integrins and growth factor receptors","volume":"63","author":"Ross","year":"2004","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib34","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1161\/hh1101.091862","article-title":"Integrins and the myocardium","volume":"88","author":"Ross","year":"2001","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib35","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1101\/gad.1319405","article-title":"VEGF-PLCgamma1 pathway controls cardiac contractility in the embryonic heart","volume":"19","author":"Rottbauer","year":"2005","journal-title":"Genes Dev."},{"key":"10.1016\/j.toxicon.2015.11.012_bib36","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1742-481X.2011.00776.x","article-title":"Rat skin wound healing induced by alternagin-C, a disintegrin-like, Cys-rich protein from Bothrops alternatus venom","volume":"8","author":"Sant'Ana","year":"2011","journal-title":"Int. Wound J."},{"key":"10.1016\/j.toxicon.2015.11.012_bib37","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.abb.2008.07.030","article-title":"Angiogenesis and growth factor modulation induced by alternagin C, a snake venom disintegrin-like, cysteine-rich protein on a rat skin wound model","volume":"479","author":"Sant'Ana","year":"2008","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2015.11.012_bib38","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1590\/S0100-879X2005001000007","article-title":"Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates \u03b12\u00df1 integrin-mediated cell adhesion, migration and proliferation","volume":"38","author":"Selistre-de-Araujo","year":"2005","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib39","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.3390\/toxins2112606","article-title":"Snake venom disintegrins and cell migration","volume":"2","author":"Selistre-de-Araujo","year":"2010","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2015.11.012_bib40","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1242\/jeb.02321","article-title":"Sarcolemmal ion currents and sarcoplasmic reticulum Ca2+ content in ventricular myocytes from the cold stenothermic fish, the burbot (Lota lota)","volume":"209","author":"Shiels","year":"2006","journal-title":"J.\u00a0Exp. Biol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib41","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/S1095-6433(02)00050-8","article-title":"The force-frequency relationship in fish hearts \u2013 a review","volume":"132A","author":"Shiels","year":"2002","journal-title":"Comp. Biochem. Physiol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib42","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1161\/01.RES.0000022200.71892.9F","article-title":"Role of Akt signaling in vascular homeostasis and angiogenesis","volume":"90","author":"Shiojima","year":"2002","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib43","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1101\/gad.1492806","article-title":"Regulation of cardiac growth and coronary angiogenesis by the Akt\/PKB signaling pathway","volume":"20","author":"Shiojima","year":"2006","journal-title":"Genes Dev."},{"key":"10.1016\/j.toxicon.2015.11.012_bib44","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1161\/01.RES.62.6.1223","article-title":"Effects of metabolic blockade on intracellular calcium concentration in isolated ferret ventricular muscle","volume":"62","author":"Smith","year":"1988","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib45","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1161\/CIRCRESAHA.109.209809","article-title":"Cardiac fibroblast: the renaissance cell","volume":"105","author":"Souders","year":"2009","journal-title":"Circ. Res."},{"key":"10.1016\/j.toxicon.2015.11.012_bib46","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1006\/abbi.2000.2120","article-title":"The disintegrin-like domain of the snake venom metalloprotease alternagin inhibits alpha2beta1 integrin-mediated cell adhesion","volume":"384","author":"Souza","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2015.11.012_bib47","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1152\/physiol.00009.2012","article-title":"Cardiac actions of protein kinase C isoforms","volume":"27","author":"Steinberg","year":"2012","journal-title":"Physiology"},{"key":"10.1016\/j.toxicon.2015.11.012_bib48","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.toxicon.2011.01.007","article-title":"Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells","volume":"57","author":"Teklemariam","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2015.11.012_bib49","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1016\/j.bbamcr.2006.08.025","article-title":"New perspectives on the role of SERCA2's Ca2+ affinity in cardiac function","volume":"1763","author":"Vangheluwe","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.toxicon.2015.11.012_bib50","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1242\/jeb.202.13.1763","article-title":"Na+\/Ca2+ exchange current in ventricular myocytes of fish heart: contribution to sarcolemmal Ca2+ influx","volume":"202","author":"Vornanen","year":"1999","journal-title":"J.\u00a0Exp. Biol."},{"key":"10.1016\/j.toxicon.2015.11.012_bib51","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1096\/fj.09-143180","article-title":"Cardiomyocyte VEGFR-1 activation by VEGF-B induces compensatory hypertrophy and preserves cardiac function after myocardial infarction","volume":"24","author":"Zentilin","year":"2010","journal-title":"FASEB J."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010115301380?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010115301380?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T20:19:33Z","timestamp":1653596373000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010115301380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":51,"alternative-id":["S0041010115301380"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2015.11.012","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2016,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Alternagin-C (ALT-C), a disintegrin-like protein from Rhinocerophis alternatus snake venom promotes positive inotropism and chronotropism in fish heart","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2015.11.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}