{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T05:08:33Z","timestamp":1740114513123,"version":"3.37.3"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"vor","delay-in-days":286,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1016\/j.toxicon.2016.03.002","type":"journal-article","created":{"date-parts":[[2016,3,8]],"date-time":"2016-03-08T02:45:35Z","timestamp":1457405135000},"page":"49-54","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Expression and partial biochemical characterization of a recombinant serine protease from Bothrops pauloensis snake venom"],"prefix":"10.1016","volume":"115","author":[{"given":"Thais F.","family":"Isabel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme Nunes Moreira","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabela B.","family":"Pacheco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana G.","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1974-1736","authenticated-orcid":false,"given":"C\u00e9lio D.","family":"Santos-Junior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando P.P.","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johara","family":"Boldrini Fran\u00e7a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio","family":"Henrique-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly A.G.","family":"Yoneyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata S.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veridiana de Melo","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2016.03.002_bib1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1080\/15569540701209815","article-title":"Expression of snake venom toxins in Pichia pastoris","volume":"26","author":"Anangi","year":"2007","journal-title":"Toxin Rev."},{"key":"10.1016\/j.toxicon.2016.03.002_bib2","first-page":"727","article-title":"Biochemical and biological differentiation of the venoms of the lancehead vipers (Bothrops marajoensis and Bothrops moojeni)","volume":"102","author":"Assakura","year":"1992","journal-title":"Compend. Biochem. Physiol."},{"key":"10.1016\/j.toxicon.2016.03.002_bib3","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1006\/abbi.1995.1227","article-title":"Families and clans of serine peptidases","volume":"318","author":"Barrett","year":"1995","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2016.03.002_bib4","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0168-1656(02)00157-8","article-title":"High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris","volume":"9","author":"Boettner","year":"2002","journal-title":"J.\u00a0Biotechnol."},{"key":"10.1016\/j.toxicon.2016.03.002_bib5","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/S0041-0101(02)00140-X","article-title":"Purification, characterization and crystallization of jararacussin-I, a fibrinogen-clotting enzyme isolated from the venom of Bothrops jararacussu","volume":"40","author":"Bortoleto","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib6","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A\u00a0rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2016.03.002_bib7","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/S0300-9084(00)01178-0","article-title":"Snake venom proteins acting on hemostasis","volume":"82","author":"Braud","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2016.03.002_bib8","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.toxicon.2009.05.040","article-title":"Biochemical and functional characterization of a new thrombin-like enzyme isolated of Bothrops pauloensis snake venom","volume":"54","author":"Costa","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib9","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/jmr.687","article-title":"Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production","volume":"18","author":"Daly","year":"2005","journal-title":"J.\u00a0Mol. Recognit."},{"key":"10.1016\/j.toxicon.2016.03.002_bib10","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.4238\/2012.May.21.11","article-title":"Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum(Hypocreaceae) in the yeast Pichia pastoris","volume":"11","author":"Generoso","year":"2012","journal-title":"Genet. Mol. Res."},{"key":"10.1016\/j.toxicon.2016.03.002_bib11","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1110\/ps.04746804","article-title":"On the modeling of snake venom proteinase interactions with benzamidine-based thrombin inhibitors","volume":"13","author":"Henriques","year":"2004","journal-title":"Protein Sci."},{"key":"10.1016\/j.toxicon.2016.03.002_bib12","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.biochi.2006.10.010","article-title":"Purification and partial characterization of two phospholipases A2 from Bothrops leucurus ( white-tailed jararaca ) snake venom","volume":"89","author":"Higuchi","year":"2007","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2016.03.002_bib13","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s00253-010-2447-0","article-title":"Engineering of protein secretion in yeast: strategies and impact on protein production","volume":"86","author":"Idiris","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.toxicon.2016.03.002_bib14","doi-asserted-by":"crossref","first-page":"3132","DOI":"10.1016\/S0021-9258(18)61479-6","article-title":"Molecular cloning and sequence analysis of cDNA for batroxobin, a thrombin-like snake venom enzyme","volume":"262","author":"Itoh","year":"1987","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2016.03.002_bib15","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0041-0101(00)00169-0","article-title":"Biochemical characterization of a thrombin-like enzyme and a fibrinolytic serine protease from snake (Agkistrodon saxatilis) venom","volume":"39","author":"Koh","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib46","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0378-1119(02)01080-6","article-title":"A survey of gene expression and diversity in the venom glands of the pitviper snake Bothrops insularis, through the generation of expressed sequence tags (ESTs)","volume":"299","author":"Junqueira-de-Azevedo","year":"2002","journal-title":"Gene"},{"key":"10.1016\/j.toxicon.2016.03.002_bib16","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0163-7258(85)90008-7","article-title":"The influence of snake venom enzymes on blood coagulation","volume":"29","author":"Kornalik","year":"1985","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.toxicon.2016.03.002_bib17","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2016.03.002_bib19","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1093\/glycob\/cwq195","article-title":"Terminal desialylated multiantennary complex-type N-glycans carried on acutobin define the glycosylation characteristics of the Deinagkistrodon acutus venom","volume":"21","author":"Lin","year":"2011","journal-title":"Glycobiology"},{"key":"10.1016\/j.toxicon.2016.03.002_bib20","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1093\/oxfordjournals.jbchem.a123432","article-title":"Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme","volume":"109","author":"Maeda","year":"1991","journal-title":"J.\u00a0Biochem."},{"key":"10.1016\/j.toxicon.2016.03.002_bib21","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0041-0101(98)00126-3","article-title":"Snake venoms and the hemostatic system","volume":"36","author":"Markland","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib22","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/S0167-4838(99)00268-X","article-title":"Snake venom proteases affecting hemostasis and thrombosis","volume":"1477","author":"Matsui","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"issue":"12","key":"10.1016\/j.toxicon.2016.03.002_bib23","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1016\/j.biochi.2012.07.007","article-title":"Biochemical characterization and comparative analysis of two distinct serine proteases from Bothrops pirajai snake venom","volume":"94","author":"Menaldo","year":"2012","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2016.03.002_bib24","first-page":"791","article-title":"Structure and activation of trypsinogen and chymotrypsinogen","volume":"16","author":"Neurath","year":"1957","journal-title":"Fed. Proc."},{"key":"10.1016\/j.toxicon.2016.03.002_bib26","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/S0041-0101(02)00363-X","article-title":"Purification and characterization of a thrombin like enzyme, elegaxobin II, with Lys-bradykinin releasing activity from the venom of Trimeresurus elegans (Sakishima Habu)","volume":"41","author":"Oyama","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib27","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1007\/s00018-008-7565-9","article-title":"Serine peptidases: classification, structure and function","volume":"65","author":"Page","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.toxicon.2016.03.002_bib28","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1055\/s-0037-1614965","article-title":"Thrombin-like enzymes from snake venoms: an updated inventory","volume":"79","author":"Pirkle","year":"1998","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.toxicon.2016.03.002_bib29","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1093\/nar\/gkr987","article-title":"Merops: the database of proteolytic enzymes, their substrates and inhibitors","volume":"40","author":"Rawlings","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.toxicon.2016.03.002_bib30","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1006\/abbi.2000.1958","article-title":"Structural and functional characterization of neuwiedase, a non hemorrhagic fibrin(ogen)olytic metalloprotease from Bothrops neuwiedi Snake venom","volume":"381","author":"Rodrigues","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"issue":"9","key":"10.1016\/j.toxicon.2016.03.002_bib31","doi-asserted-by":"crossref","first-page":"2707","DOI":"10.1016\/j.jprot.2012.03.028","article-title":"Combined snake venomics and venom gland transcriptomic analysis of Bothropoides pauloensis","volume":"75","author":"Rodrigues","year":"2012","journal-title":"J.\u00a0Proteom."},{"volume":"3 vol.","year":"2001","author":"Sambrook","key":"10.1016\/j.toxicon.2016.03.002_bib33"},{"key":"10.1016\/j.toxicon.2016.03.002_bib34","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2174\/157341207780598968","article-title":"Proteases from south American snake venoms affecting fibrinolysis","volume":"3","author":"Sanchez","year":"2007","journal-title":"Curr. Pharm. Anal."},{"key":"10.1016\/j.toxicon.2016.03.002_bib36","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0041-0101(90)90062-C","article-title":"Isolation and characterization of hemorrhagic, myonecrotic and edema-inducing toxins from Bothrops insularis (jararaca ilhoa) snake venon","volume":"28","author":"Selistre","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib37","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1006\/prep.2000.1225","article-title":"Expression, refolding, and activity of a recombinant nonhemorrhagic snake venom metalloprotease","volume":"19","author":"Selistre-De-Araujo","year":"2000","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2016.03.002_bib38","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.toxicon.2005.02.020","article-title":"Snake venom serine proteinases: sequence homology vs. substrate specificity, a paradox to be solved","volume":"45","author":"Serrano","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib39","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.toxicon.2012.09.003","article-title":"The long road of research on snake venom serine proteinases","volume":"62","author":"Serrano","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib40","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S0076-6879(76)45021-8","article-title":"The coagulant enzyme from Bothrops atrox venom (batroxobin)","volume":"45","author":"Stocker","year":"1976","journal-title":"Methods Enzym."},{"key":"10.1016\/j.toxicon.2016.03.002_bib41","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.toxicon.2005.02.027","article-title":"Snake venom fibrin(ogen)olytic enzymes","volume":"45","author":"Swenson","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib47","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1016\/S0006-2952(01)00800-0","article-title":"Inhibitory properties of the anti-bothropic complex from Didelphis albiventris serum on toxic and pharmacological actions of metalloproteases and myotoxins from Bothrops asper venom","volume":"62","author":"Trento","year":"2001","journal-title":"Biochem. Pharmacol"},{"key":"10.1016\/j.toxicon.2016.03.002_bib42","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1042\/bj3540161","article-title":"Serine protease isoforms of Deinagkistrodon acutus venom: cloning, sequencing and phylogenetic analysis","volume":"354","author":"Wang","year":"2001","journal-title":"Biochem. J."},{"key":"10.1016\/j.toxicon.2016.03.002_bib43","first-page":"6","article-title":"Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis","volume":"34","author":"Yang","year":"2002","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"10.1016\/j.toxicon.2016.03.002_bib44","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.febslet.2004.06.060","article-title":"Functional characterization of recombinant batroxobin, a snake venom thrombin-like enzyme, expressed from Pichia pastoris","volume":"571","author":"You","year":"2004","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2016.03.002_bib48","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.toxicon.2009.03.022","article-title":"Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional Gyroxin homologue in COS-7 cells","volume":"54","author":"Yonamine","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.03.002_bib45","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.pep.2007.07.002","article-title":"Expression and purification of Ancrod, an anticoagulant drug, in Pichia pastoris","volume":"55","author":"Yu","year":"2007","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.toxicon.2016.03.002_bib49","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1358\/mf.2003.25.4.769672","article-title":"cDNA cloning and high-level expression of a thrombin-like enzyme from Agkistrodon acutus venom","volume":"25","author":"Zha","year":"2003","journal-title":"Methods Find. Exp. Clin. Pharmacol"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010116300423?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010116300423?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T13:42:50Z","timestamp":1617716570000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010116300423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":45,"alternative-id":["S0041010116300423"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2016.03.002","relation":{},"ISSN":["0041-0101"],"issn-type":[{"type":"print","value":"0041-0101"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expression and partial biochemical characterization of a recombinant serine protease from Bothrops pauloensis snake venom","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2016.03.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}