{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T20:25:22Z","timestamp":1781727922565,"version":"3.54.5"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,7,28]],"date-time":"2017-07-28T00:00:00Z","timestamp":1501200000000},"content-version":"vor","delay-in-days":330,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["063\/2010"],"award-info":[{"award-number":["063\/2010"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005670","name":"FAPERN","doi-asserted-by":"publisher","award":["003\/2011"],"award-info":[{"award-number":["003\/2011"]}],"id":[{"id":"10.13039\/501100005670","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["400743\/2013-2\/"],"award-info":[{"award-number":["400743\/2013-2\/"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1016\/j.toxicon.2016.06.002","type":"journal-article","created":{"date-parts":[[2016,6,3]],"date-time":"2016-06-03T16:25:57Z","timestamp":1464971157000},"page":"362-370","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"special_numbering":"C","title":["Characterization of TistH, a multifunctional peptide from the scorpion Tityus stigmurus: Structure, cytotoxicity and antimicrobial activity"],"prefix":"10.1016","volume":"119","author":[{"given":"Richele J.A.","family":"Machado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9ia B.","family":"Estrela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana K.L.","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menilla M.A.","family":"Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoela","family":"Torres-R\u00eago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edeltrurdes O.","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo A.O.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eneas","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arn\u00f3bio A.","family":"Silva-Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4221-9580","authenticated-orcid":false,"given":"Matheus F.","family":"Fernandes-Pedrosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2016.06.002_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-13-362","article-title":"Profiling the resting venom gland of the scorpion Tityus stigmurus through a transcriptomic survey","volume":"13","author":"Almeida","year":"2012","journal-title":"BMC Genomics"},{"key":"10.1016\/j.toxicon.2016.06.002_bib2","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1590\/S0037-86822009000300004","article-title":"Tityus stigmurus (Thorell, 1876) (Scorpiones; Buthidae): response to chemical control and understanding of scorpionism among the population","volume":"42","author":"De Albuquerque","year":"2009","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.toxicon.2016.06.002_bib3","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1177\/1535370213513991","article-title":"Scorpion venoms as a potential source of novel cancer therapeutic compounds","volume":"239","author":"Ding","year":"2014","journal-title":"Exp. Biol. Med."},{"key":"10.1016\/j.toxicon.2016.06.002_bib4","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/S0041-0101(02)00142-3","article-title":"An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch","volume":"40","author":"Goudet","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.06.002_bib5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","article-title":"Analysis of nitrate, nitrite, and [ 15N ] nitrate in biological fluids","volume":"138","author":"Green","year":"1982","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2016.06.002_bib6","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1038\/nprot.2006.202","article-title":"Using circular dichroism spectra to estimate protein secondary structure","volume":"1","author":"Greenfield","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.toxicon.2016.06.002_bib7","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1016\/j.biochi.2013.06.003","article-title":"Two peptides, TsAP-1 and TsAP-2, from the venom of the Brazilian yellow scorpion, Tityus serrulatus: evaluation of their antimicrobial and anticancer activities","volume":"95","author":"Guo","year":"2013","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2016.06.002_bib8","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/(SICI)1099-1565(200005\/06)11:3<137::AID-PCA514>3.0.CO;2-I","article-title":"Testing of antifungal natural products: methodologies, comparability of results and assay choice","volume":"11","author":"Hadacek","year":"2000","journal-title":"Phytochem. Anal."},{"key":"10.1016\/j.toxicon.2016.06.002_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/958797","article-title":"Scorpion peptides: potential use for new drug development","volume":"2013","author":"Hmed","year":"2013","journal-title":"J.\u00a0Toxicol."},{"key":"10.1016\/j.toxicon.2016.06.002_bib10","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.jep.2014.03.060","article-title":"Ipomoea asarifolia neutralizes inflammation induced by Tityus serrulatus scorpion venom","volume":"153","author":"Lima","year":"2014","journal-title":"J.\u00a0Ethnopharmacol."},{"key":"10.1016\/j.toxicon.2016.06.002_bib11","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1590\/S1678-91992010000100004","article-title":"Some arachnidan peptides with potential medical application","volume":"16","author":"Lima","year":"2010","journal-title":"J.\u00a0Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2016.06.002_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxicon.2015.04.003","article-title":"Homology modeling, vasorelaxant and bradykinin-potentiating activities of a novel hypotensin found in the scorpion venom from Tityus stigmurus","volume":"101","author":"Machado","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.06.002_bib13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.peptides.2015.03.003","article-title":"Structural characterization of a novel peptide with antimicrobial activity from the venom gland of the scorpion Tityus stigmurus: stigmurin","volume":"68","author":"Melo","year":"2015","journal-title":"Peptides"},{"key":"10.1016\/j.toxicon.2016.06.002_bib14","series-title":"Man. Control. Escorpi\u00f5es","author":"Minist\u00e9rio da Sa\u00fade","year":"2009"},{"key":"10.1016\/j.toxicon.2016.06.002_bib15","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J.\u00a0Immunol. Methods"},{"key":"10.1016\/j.toxicon.2016.06.002_bib16","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.toxicon.2014.11.233","article-title":"Scorpion venom components as potential candidates for drug development","volume":"93","author":"Ortiz","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.06.002_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2007.08.018","article-title":"Southern African scorpion toxins: an overview","volume":"51","author":"Plessis","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.06.002_bib18","series-title":"Evaluation of Antifungal Activity and Mode of Action of New Coumarin Derivative, against Aspergillus spp. 2015","author":"Queiroga","year":"2015"},{"key":"10.1016\/j.toxicon.2016.06.002_bib19","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1016\/j.toxicon.2010.04.006","article-title":"Structure-function studies of Tityus serrulatus Hypotensin-I (TsHpt-I): a new agonist of B(2) kinin receptor","volume":"56","author":"Verano-Braga","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.06.002_bib20","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.bbrc.2008.04.104","article-title":"Tityus serrulatus Hypotensins: a new family of peptides from scorpion venom","volume":"371","author":"Verano-Braga","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.toxicon.2016.06.002_bib21","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.trim.2008.01.003","article-title":"Efficacy of tacrolimus in inhibiting inflammation caused by carrageenan in a murine model of air pouch","volume":"19","author":"Vigil","year":"2008","journal-title":"Transpl. Immunol."},{"key":"10.1016\/j.toxicon.2016.06.002_bib22","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1110\/ps.0229603","article-title":"Analyses of circular dichroism spectra of membrane proteins","volume":"12","author":"Wallace","year":"2003","journal-title":"Protein Sci."},{"key":"10.1016\/j.toxicon.2016.06.002_bib23","first-page":"1","article-title":"Review on bioactive peptides and pharmacological activities of buthus martensii karsch","volume":"04","author":"Wang","year":"2015","journal-title":"Biochem. Pharmacol. Open Access"},{"key":"10.1016\/j.toxicon.2016.06.002_bib24","doi-asserted-by":"crossref","first-page":"W668","DOI":"10.1093\/nar\/gkh371","article-title":"DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data","volume":"32","author":"Whitmore","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.toxicon.2016.06.002_bib25","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/bip.20853","article-title":"Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases","volume":"89","author":"Whitmore","year":"2008","journal-title":"Biopolymers"},{"key":"10.1016\/j.toxicon.2016.06.002_bib26","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.neuropharm.2004.12.024","article-title":"Intrathecal neostigmine reduces the zymosan-induced inflammatory response in a mouse air pouch model via adrenomedullary activity: involvement of spinal muscarinic type 2 receptors","volume":"49","author":"Yoon","year":"2005","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.toxicon.2016.06.002_bib27","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.peptides.2011.11.012","article-title":"Characterization of BmKbpp, a multifunctional peptide from the Chinese scorpion Mesobuthus martensii Karsch: gaining insight into a new mechanism for the functional diversification of scorpion venom peptides","volume":"33","author":"Zeng","year":"2012","journal-title":"Peptides"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010116301568?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010116301568?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T16:12:52Z","timestamp":1572624772000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010116301568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":27,"alternative-id":["S0041010116301568"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2016.06.002","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of TistH, a multifunctional peptide from the scorpion Tityus stigmurus: Structure, cytotoxicity and antimicrobial activity","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2016.06.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}