{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:06:20Z","timestamp":1775235980491,"version":"3.50.1"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["15-04-01843"],"award-info":[{"award-number":["15-04-01843"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"name":"President of the Russian Federation","award":["\u041c\u041a-6216.2016.4"],"award-info":[{"award-number":["\u041c\u041a-6216.2016.4"]}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["14-24-00118"],"award-info":[{"award-number":["14-24-00118"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.toxicon.2016.08.020","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T07:29:54Z","timestamp":1472801394000},"page":"70-76","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Peptides from puff adder Bitis arietans venom, novel inhibitors of nicotinic acetylcholine receptors"],"prefix":"10.1016","volume":"121","author":[{"given":"Catherine A.","family":"Vulfius","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina N.","family":"Spirova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina V.","family":"Serebryakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina V.","family":"Shelukhina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis S.","family":"Kudryavtsev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena V.","family":"Kryukova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav G.","family":"Starkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina V.","family":"Kopylova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxim N.","family":"Zhmak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor A.","family":"Ivanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ksenia S.","family":"Kudryashova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana V.","family":"Andreeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor I.","family":"Tsetlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4609-970X","authenticated-orcid":false,"given":"Yuri N.","family":"Utkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2016.08.020_bib1","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/S0022-3565(24)36503-6","article-title":"Properties of neuronal nicotinic acetylcholine receptors: pharmacological characterization and modulation of synaptic function","volume":"280","author":"Albuquerque","year":"1997","journal-title":"J.\u00a0Pharmacol. Exp. Ther."},{"key":"10.1016\/j.toxicon.2016.08.020_bib2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1007\/BF00306989","article-title":"Golgi-Cox studies on the central nervous system of a gastropod mollusk","volume":"128","author":"Benjamin","year":"1972","journal-title":"Z.\u00a0Zellforsch"},{"key":"10.1016\/j.toxicon.2016.08.020_bib3","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1146\/annurev.pharmtox.40.1.431","article-title":"Nicotinic receptors at the amino acid level","volume":"40","author":"Corringer","year":"2000","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.toxicon.2016.08.020_bib4","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1146\/annurev.pharmtox.47.120505.105214","article-title":"Nicotinic acetylcholine receptors and nicotinic cholinergic mechanisms of the central nervous system","volume":"47","author":"Dani","year":"2007","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.toxicon.2016.08.020_bib5","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.tips.2014.12.002","article-title":"Nicotinic ACh receptors as therapeutic targets in CNS disorders","volume":"36","author":"Dineley","year":"2015","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.toxicon.2016.08.020_bib6","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1074\/jbc.M204565200","article-title":"Alpha-conotoxins ImI and ImII. Similar alpha 7 nicotinic receptor antagonists act at different sites","volume":"278","author":"Ellison","year":"2003","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib7","doi-asserted-by":"crossref","first-page":"16019","DOI":"10.1021\/bi048918g","article-title":"Alpha-conotoxins ImI and ImII target distinct regions of the human alpha7 nicotinic acetylcholine receptor and distinguish human nicotinic receptor subtypes","volume":"43","author":"Ellison","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.toxicon.2016.08.020_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-319-13665-3_1","article-title":"Structure of neuronal nicotinic receptors","volume":"23","author":"Fasoli","year":"2015","journal-title":"Curr. Top. Behav. Neurosci."},{"key":"10.1016\/j.toxicon.2016.08.020_bib9","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.bcp.2009.05.024","article-title":"Structural and functional diversity of native brain neuronal nicotinic receptors","volume":"78","author":"Gotti","year":"2010","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.toxicon.2016.08.020_bib10","doi-asserted-by":"crossref","first-page":"22254","DOI":"10.1074\/jbc.M110.111880","article-title":"\u03b1-Conotoxin AuIB isomers exhibit distinct inhibitory mechanisms and differential sensitivity to stoichiometry of \u03b13\u03b24 nicotinic acetylcholine receptors","volume":"285","author":"Grishin","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib11","first-page":"51","volume":"39","author":"International Guiding Principles for Animal Research","year":"1985","journal-title":"WHO Chron."},{"key":"10.1016\/j.toxicon.2016.08.020_bib12","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nrn731","article-title":"Emerging structure of the nicotinic acetylcholine receptors","volume":"3","author":"Karlin","year":"2002","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.toxicon.2016.08.020_bib13","doi-asserted-by":"crossref","first-page":"4470","DOI":"10.1111\/j.1742-4658.2006.05453.x","article-title":"\u03b1-conotoxin analogs with additional positive charge show increased selectivity towards Torpedo californica and some neuronal subtypes of nicotinic acetylcholine receptors","volume":"273","author":"Kasheverov","year":"2006","journal-title":"FEBS J."},{"key":"10.1016\/j.toxicon.2016.08.020_bib14","doi-asserted-by":"crossref","first-page":"2430","DOI":"10.2174\/138161209788682316","article-title":"Naturally occurring and synthetic peptides acting on nicotinic acetylcholine receptors","volume":"15","author":"Kasheverov","year":"2009","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.toxicon.2016.08.020_bib15","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1111\/j.1471-4159.2009.06359.x","article-title":"Interaction of alpha-conotoxin ImII and its analogs with nicotinic receptors and acetylcholine-binding proteins: additional binding sites on Torpedo receptor","volume":"111","author":"Kasheverov","year":"2009","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib16","doi-asserted-by":"crossref","first-page":"8198","DOI":"10.1523\/JNEUROSCI.18-20-08198.1998","article-title":"Two distinct nicotinic receptors, one pharmacologically similar to the vertebrate \u03b17-containing receptor, mediate Cl currents in Aplysia neurons","volume":"18","author":"Kehoe","year":"1998","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.toxicon.2016.08.020_bib17","doi-asserted-by":"crossref","first-page":"20060","DOI":"10.1074\/jbc.M115.647040","article-title":"Identification and characterization of a G protein-binding cluster in \u03b17 nicotinic acetylcholine receptors","volume":"290","author":"King","year":"2015","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib18","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0165-0270(81)90032-7","article-title":"Intracellular perfusion","volume":"4","author":"Kostyuk","year":"1981","journal-title":"J.\u00a0Neurosci. Methods"},{"key":"10.1016\/j.toxicon.2016.08.020_bib19","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/S0022-3565(24)38167-4","article-title":"Waglerin-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor","volume":"289","author":"McArdle","year":"1999","journal-title":"J.\u00a0Pharmacol. Exp. Ther."},{"key":"10.1016\/j.toxicon.2016.08.020_bib20","series-title":"Acetylcholine. An Approach to the Molecular Machanism of Action","first-page":"73","author":"Michelson","year":"1973"},{"key":"10.1016\/j.toxicon.2016.08.020_bib21","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1042\/bst0310869","article-title":"Assembly and subunit diversity of nicotinic acetylcholine receptors","volume":"31","author":"Millar","year":"2003","journal-title":"Bioch. Soc. Trans."},{"key":"10.1016\/j.toxicon.2016.08.020_bib22","doi-asserted-by":"crossref","first-page":"5433","DOI":"10.1074\/jbc.M109232200","article-title":"Identification of residues at the alpha and epsilon subunit interfaces mediating species selectivity of Waglerin-1 for nicotinic acetylcholine receptors","volume":"277","author":"Molles","year":"2002","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib23","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1111\/j.1432-1033.2004.04145.x","article-title":"\u03b1-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic receptor subtypes","volume":"271","author":"Nicke","year":"2004","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bcp.2014.01.029","article-title":"Merging old and new perspectives on nicotinic acetylcholine receptors","volume":"89","author":"Papke","year":"2014","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.toxicon.2016.08.020_bib25","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1255\/ejms.792","article-title":"Mass spectrometric sequencing and acylation character analysis of C-terminal anchoring segment from Influenza A hemagglutinin","volume":"12","author":"Serebryakova","year":"2006","journal-title":"Eur. J. Mass Spectrom. (Chichester)"},{"key":"10.1016\/j.toxicon.2016.08.020_bib26","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1021\/ac00229a008","article-title":"Direct determination of absolute circular dichroism data and calibration of commercial instruments","volume":"53","author":"Schippers","year":"1981","journal-title":"Anal. Chem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib27","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0014-5793(03)01454-6","article-title":"Snake and snail toxins acting on nicotinic acetylcholine receptors: fundamental aspects and medical applications","volume":"557","author":"Tsetlin","year":"2004","journal-title":"FEBS Lett."},{"key":"10.1016\/j.toxicon.2016.08.020_bib28","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.bcp.2009.05.032","article-title":"Polypeptide and peptide toxins, magnifying lenses for binding sites in nicotinic acetylcholine receptors","volume":"78","author":"Tsetlin","year":"2009","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.toxicon.2016.08.020_bib29","doi-asserted-by":"crossref","first-page":"27079","DOI":"10.1074\/jbc.M112.363051","article-title":"Azemiopsin from Azemiops feae viper venom, a novel polypeptide ligand of nicotinic acetylcholine receptor","volume":"287","author":"Utkin","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2016.08.020_bib30","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.neulet.2004.10.010","article-title":"Diversity of nicotinic receptors mediating Cl- current in Lymnaea neurons distinguished with specific agonists and antagonist","volume":"373","author":"Vulfius","year":"2005","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.toxicon.2016.08.020_bib31","first-page":"111","article-title":"\u03b17-subtype of neuronal nicotinic acetylcholine receptors: structure, properties, distribution, functions","volume":"23","author":"Vulfius","year":"2006","journal-title":"Biol. Membr."},{"key":"10.1016\/j.toxicon.2016.08.020_bib32","series-title":"Proc. Of International Conference \u201cReceptors and Intracellular Signaling\u201d, Pushchino 2009, v.1","first-page":"345","article-title":"Polypeptide antagonists of nicotinic acetylcholine receptors and Ca2+ channels from venoms of snakes of Viperidae family","author":"Vulfius","year":"2009"},{"key":"10.1016\/j.toxicon.2016.08.020_bib33","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.toxicon.2011.02.013","article-title":"An unusual phospholipase A2 from puff adder Bitis arietans venom\u2013a novel blocker of nicotinic acetylcholine receptors","volume":"57","author":"Vulfius","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2016.08.020_bib34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0140-6736(09)61754-2","article-title":"Snake bite","volume":"375","author":"Warrell","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.toxicon.2016.08.020_bib35","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1385\/JMN:30:1:137","article-title":"Nicotinic receptors modulate transmitter cross talk in the CNS: nicotinic modulation of transmitters","volume":"30","author":"Wonnacott","year":"2006","journal-title":"J.\u00a0Mol. Neurosci."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010116302616?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010116302616?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T16:07:22Z","timestamp":1749571642000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010116302616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":35,"alternative-id":["S0041010116302616"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2016.08.020","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Peptides from puff adder Bitis arietans venom, novel inhibitors of nicotinic acetylcholine receptors","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2016.08.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}