{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:21:15Z","timestamp":1782940875910,"version":"3.54.5"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"central fiscal investment"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.toxicon.2017.02.021","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T21:13:14Z","timestamp":1488489194000},"page":"79-86","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Preparation and characterization of a human scFv against the Clostridium perfringens type A alpha-toxin"],"prefix":"10.1016","volume":"130","author":[{"given":"Dongdong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhuan","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangmou","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuangang","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinpeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongrong","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyuan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.toxicon.2017.02.021_bib1","doi-asserted-by":"crossref","first-page":"552","DOI":"10.4161\/mabs.1.6.10057","article-title":"Human monomeric antibody fragments to trail-r1 and trail-r2 that display potent in\u00a0vitro agonism","volume":"1","author":"Dobson","year":"2008","journal-title":"mAbs"},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.toxicon.2014.06.012","article-title":"Prevention and treatment of clostridium perfringens epsilon toxin intoxication in mice with a neutralizing monoclonal antibody (c4d7) produced in nicotiana benthamiana","volume":"88","author":"Garcia","year":"2014","journal-title":"Toxicon"},{"issue":"1","key":"10.1016\/j.toxicon.2017.02.021_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1746-6148-10-32","article-title":"Clostridium perfringens strains from bovine enterotoxemia cases are not superior in in\u00a0vitro production of alpha toxin, perfringolysin o and proteolytic enzymes","volume":"10","author":"Goossens","year":"2014","journal-title":"BMC Veterinary Res."},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib4","first-page":"323","article-title":"Necrotizing soft tissue infections: a primary care review","volume":"68","author":"Headley","year":"2003","journal-title":"Am. Fam. Physician"},{"issue":"11","key":"10.1016\/j.toxicon.2017.02.021_bib5","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1128\/IAI.00686-08","article-title":"Recombinant bacillus subtilis expressing the clostridium perfringens alpha toxoid is a candidate orally delivered vaccine against necrotic enteritis","volume":"76","author":"Hoang","year":"2008","journal-title":"Infect. Immun."},{"issue":"10","key":"10.1016\/j.toxicon.2017.02.021_bib6","first-page":"1258","article-title":"Prokaryotic expression and purification of clostridium perfringens alpha toxin","volume":"27","author":"Jia","year":"2014","journal-title":"Chin. J. Biol."},{"issue":"1\u20132","key":"10.1016\/j.toxicon.2017.02.021_bib7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0022-1759(96)00188-3","article-title":"High level production of soluble single chain antibodies in small-scale escherichia coli cultures","volume":"200","author":"Kipriyanov","year":"1997","journal-title":"J.\u00a0Immunol. Methods"},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib8","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/MMBR.00062-12","article-title":"Toxin plasmids of clostridium perfringens","volume":"77","author":"Li","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev. Mmbr"},{"issue":"12","key":"10.1016\/j.toxicon.2017.02.021_bib9","doi-asserted-by":"crossref","first-page":"4338","DOI":"10.1128\/IAI.59.12.4338-4342.1991","article-title":"Epitope mapping of the alpha-toxin of clostridium perfringens","volume":"59","author":"Logan","year":"1991","journal-title":"Infect. Immun."},{"issue":"8","key":"10.1016\/j.toxicon.2017.02.021_bib10","first-page":"543","article-title":"Therapeutic bispecific antibodies: the selection of stable single-chain fragments to overcome engineering obstacles","volume":"13","author":"Mabry","year":"2010","journal-title":"Idrugs Investigational Drugs J."},{"issue":"5","key":"10.1016\/j.toxicon.2017.02.021_bib11","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/1348-0421.12036","article-title":"A\u00a0recombinant carboxy-terminal domain of alpha-toxin protects mice against clostridium perfringens","volume":"57","author":"Nagahama","year":"2013","journal-title":"Microbiol. Immunol."},{"issue":"39","key":"10.1016\/j.toxicon.2017.02.021_bib12","doi-asserted-by":"crossref","first-page":"33070","DOI":"10.1074\/jbc.M112.393801","article-title":"Clostridium perfringens alpha-toxin recognizes the gm1a-trka complex","volume":"287","author":"Oda","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib13","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.imlet.2015.07.005","article-title":"A\u00a0new expression vector facilitating production and functional analysis of scfv antibody fragments selected from tomlinson i+j phagemid libraries","volume":"167","author":"Ossysek","year":"2015","journal-title":"Immunol. Lett."},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib14","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.imlet.2015.07.005","article-title":"A\u00a0new expression vector facilitating production and functional analysis of scfv antibody fragments selected from tomlinson i+j phagemid libraries","volume":"167","author":"Ossysek","year":"2015","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.toxicon.2017.02.021_bib15","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/978-1-61779-974-7_26","article-title":"Affinity maturation of antibodies: optimized methods to generate high-quality scfv libraries and isolate igg candidates by high-throughput screening","volume":"907","author":"Renaut","year":"2012","journal-title":"Methods Mol. Biol."},{"issue":"1","key":"10.1016\/j.toxicon.2017.02.021_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/mpat.1996.0123","article-title":"Neutralization of hemolytic and mouse lethal activities of c. perfringens alpha-toxin need simultaneous blockade of two epitopes by monoclonal antibodies","volume":"23","author":"Schoepe","year":"1997","journal-title":"Microb. Pathog."},{"issue":"1","key":"10.1016\/j.toxicon.2017.02.021_bib17","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.anaerobe.2005.06.003","article-title":"Immunization with an alphatoxin variant 121a\/91-r212h protects mice against clostridium perfringens, alphatoxin","volume":"12","author":"Schoepe","year":"2006","journal-title":"Anaerobe"},{"issue":"1","key":"10.1016\/j.toxicon.2017.02.021_bib18","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.molimm.2015.01.005","article-title":"Immunization with recombinant bivalent chimera r-cpae confers protection against alpha toxin and enterotoxin of clostridium perfringens, type a in murine model","volume":"65","author":"Shreya","year":"2015","journal-title":"Mol. Immunol."},{"issue":"6","key":"10.1016\/j.toxicon.2017.02.021_bib19","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.bios.2014.04.025","article-title":"Surface plasmon resonance characterization of monoclonal and polyclonal antibodies of malaria for biosensor applications","volume":"60","author":"Sikarwar","year":"2014","journal-title":"Biosens. Bioelectron."},{"issue":"4","key":"10.1016\/j.toxicon.2017.02.021_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0120497","article-title":"Clostridium perfringens alpha-toxin induces gm1a clustering and trka phosphorylation in the host cell membrane","volume":"10","author":"Takagishi","year":"2015","journal-title":"Plos One"},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib21","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/j.1365-2672.2012.05333.x","article-title":"Generation and characterization of an inter-generic bivalent alpha domain fusion protein \u03b1cs from clostridium perfringens, and staphylococcus aureus, for concurrent diagnosis and therapeutic applications","volume":"113","author":"Uppalapati","year":"2012","journal-title":"J.\u00a0Appl. Microbiol."},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib23","doi-asserted-by":"crossref","first-page":"24","DOI":"10.2174\/1875414701003020024","article-title":"Clostridium perfringens toxins involved in mammalian veterinary diseases","volume":"2","author":"Uzal","year":"2010","journal-title":"Open Toxinology J."},{"issue":"3","key":"10.1016\/j.toxicon.2017.02.021_bib24","doi-asserted-by":"crossref","first-page":"361","DOI":"10.2217\/fmb.13.168","article-title":"Towards an understanding of the role of clostridium perfringens toxins in human and animal disease","volume":"9","author":"Uzal","year":"2014","journal-title":"Future Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.toxicon.2017.02.021_bib25","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.vetmic.2015.02.013","article-title":"Animal models to study the pathogenesis of human and animal clostridium perfringens infections","volume":"179","author":"Uzal","year":"2015","journal-title":"Veterinary Microbiol."},{"issue":"11","key":"10.1016\/j.toxicon.2017.02.021_bib26","doi-asserted-by":"crossref","first-page":"3320","DOI":"10.1038\/srep03320","article-title":"Isolation, identification and expression of specific human cd133 antibodies","volume":"3","author":"Xia","year":"2013","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.toxicon.2017.02.021_bib27","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s10616-007-9072-5","article-title":"Recent advances in the generation of human monoclonal antibody","volume":"55","author":"Yamashita","year":"2007","journal-title":"Cytotechnology"},{"issue":"3","key":"10.1016\/j.toxicon.2017.02.021_bib28","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.thromres.2004.06.013","article-title":"Generation and characterization of a novel single-chain antibody fragment specific against human fibrin clots from phage display antibody library","volume":"114","author":"Yan","year":"2004","journal-title":"Thrombosis Res."},{"issue":"24","key":"10.1016\/j.toxicon.2017.02.021_bib29","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.11569\/wcjd.v14.i24.2395","article-title":"Construction and expression of bivalent single-chain antibody against human Colorectal carcinoma","volume":"14","author":"Yan","year":"2006","journal-title":"World Chin. J. Dig."},{"issue":"1","key":"10.1016\/j.toxicon.2017.02.021_bib30","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1128\/AEM.63.1.263-269.1997","article-title":"Molecular cloning, expression, and characterization of a functional single-chain fv antibody to the mycotoxin zearalenone","volume":"63","author":"Yuan","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"issue":"6","key":"10.1016\/j.toxicon.2017.02.021_bib31","first-page":"690","article-title":"Molecular cloning, expression and biological activity of a functional scfv to the alpha-toxin of clostridium perfringens type A","volume":"19","author":"Zhao","year":"2003","journal-title":"Chin. J. Biochem. Mol. Biol."}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010117300673?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010117300673?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T05:19:53Z","timestamp":1601702393000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010117300673"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":30,"alternative-id":["S0041010117300673"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2017.02.021","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preparation and characterization of a human scFv against the Clostridium perfringens type A alpha-toxin","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2017.02.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}