{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:21:31Z","timestamp":1791354091936,"version":"4.3.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1016\/j.toxicon.2017.10.005","type":"journal-article","created":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T16:17:20Z","timestamp":1507825040000},"page":"87-93","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["An improved technique for the assessment of venom-induced haemorrhage in a murine model"],"prefix":"10.1016","volume":"139","author":[{"given":"Timothy P.","family":"Jenkins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro","family":"Segura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u00e1ngela","family":"Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0778-8059","authenticated-orcid":false,"given":"Trenton K.","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8385-3081","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2017.10.005_bib1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.toxicon.2017.04.001","article-title":"Peptidomimetic hydroxamate metalloproteinase inhibitors abrogate local and systemic toxicity induced by Echis ocellatus (saw scaled) snake venom","volume":"132","author":"Arias","year":"2017","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0001670","article-title":"Consequences of neglect: analysis of the sub-saharan African snake antivenom market and the global context","volume":"6","author":"Brown","year":"2012","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2017.10.005_bib3","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1586\/epr.11.61","article-title":"Proteomic tools against the neglected pathology of snake bite envenoming","volume":"8","author":"Calvete","year":"2011","journal-title":"Expert Rev. Proteom."},{"key":"10.1016\/j.toxicon.2017.10.005_bib4","doi-asserted-by":"crossref","first-page":"9205","DOI":"10.1073\/pnas.1405484111","article-title":"Medically important differences in snake venom composition are dictated by distinct postgenomic mechanisms","volume":"111","author":"Casewell","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.toxicon.2017.10.005_bib5","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/0041-0101(91)90116-9","article-title":"Snake venom variability: methods of study, results and interpretation","volume":"29","author":"Chippaux","year":"1991","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib6","first-page":"515","article-title":"Snake-bites: appraisal of the global situation","volume":"76","author":"Chippaux","year":"1998","journal-title":"Bull. World Health Organ"},{"key":"10.1016\/j.toxicon.2017.10.005_bib7","series-title":"The International Guiding Principles for Biomedical Research Involving Animals","author":"CIOMS (Council of International Organizations of Medical Sciences)","year":"1985"},{"key":"10.1016\/j.toxicon.2017.10.005_bib8","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/S0041-0101(99)00205-6","article-title":"A\u00a0comparison of different methods to assess the hemorrhagic activity of Bothrops venoms","volume":"38","author":"De Roodt","year":"2000","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib9","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1016\/j.jprot.2011.03.026","article-title":"Key events in microvascular damage induced by snake venom hemorrhagic metalloproteinases","volume":"74","author":"Escalante","year":"2011","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2017.10.005_bib10","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/0041-0101(85)90380-0","article-title":"Neutralization of proteolytic and hemorrhagic activities of Costa Rican snake venoms by a polyvalent antivenom","volume":"23","author":"Guti\u00e9rrez","year":"1985","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib11","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/0041-0101(90)90110-S","article-title":"Standardization of assays for testing the neutralizing ability of antivenoms","volume":"28","author":"Guti\u00e9rrez","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib12","series-title":"Envenomings and Their Treatments","first-page":"223","article-title":"Evaluation of the neutralizing ability of antivenoms for the treatment of snakebite envenoming in Central America","author":"Guti\u00e9rrez","year":"1996"},{"key":"10.1016\/j.toxicon.2017.10.005_bib13","doi-asserted-by":"crossref","first-page":"0727","DOI":"10.1371\/journal.pmed.0030150","article-title":"Confronting the neglected problem of snake bite envenoming: the need for a global partnership","volume":"3","author":"Guti\u00e9rrez","year":"2006","journal-title":"PLoS Med."},{"key":"10.1016\/j.toxicon.2017.10.005_bib14","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.toxicon.2012.11.016","article-title":"Assessing the preclinical efficacy of antivenoms: from the lethality neutralization assay to antivenomics","volume":"69","author":"Guti\u00e9rrez","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib15","doi-asserted-by":"crossref","first-page":"E163","DOI":"10.3390\/toxins9050163","article-title":"Preclinical evaluation of the efficacy of antivenoms for snakebite envenoming: state-of-the-art and challenges ahead","volume":"9","author":"Guti\u00e9rrez","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2017.10.005_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0000569","article-title":"Snake envenoming: a disease of poverty","volume":"3","author":"Harrison","year":"2009","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2017.10.005_bib17","series-title":"Multimedia Systems and Equipment-Color Measurement and Management - Part 2-1","author":"International Electrotechnical Commission (IEC)","year":"2006"},{"key":"10.1016\/j.toxicon.2017.10.005_bib18","doi-asserted-by":"crossref","first-page":"e218","DOI":"10.1371\/journal.pmed.0050218","article-title":"The global burden of snakebite: a literature analysis and modelling based on regional estimates of envenoming and deaths","volume":"5","author":"Kasturiratne","year":"2008","journal-title":"PLoS Med."},{"key":"10.1016\/j.toxicon.2017.10.005_bib19","doi-asserted-by":"crossref","first-page":"43","DOI":"10.7883\/yoken1952.13.43","article-title":"Studies on the quantitative method for determination of hemorrhagic activity of Habu snake venom","volume":"13","author":"Kondo","year":"1960","journal-title":"Jpn. J. Med. Sci. Biol."},{"key":"10.1016\/j.toxicon.2017.10.005_bib20","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1081\/CLT-120021113","article-title":"Snake antivenoms","volume":"41","author":"Lalloo","year":"2003","journal-title":"J.\u00a0Toxicol. Clin. Toxicol."},{"key":"10.1016\/j.toxicon.2017.10.005_bib21","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0041-0101(84)90023-0","article-title":"A\u00a0new method for quantitating hemorrhage induced by rattlesnake venoms: ability of polyvalent antivenom to neutralize hemorrhagic activity","volume":"22","author":"Ownby","year":"1984","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib22","series-title":"International Conference on Scale Space and Variational Methods in Computer Vision","first-page":"413","article-title":"Luminance-hue specification in the RGB space","author":"Pierre","year":"2015"},{"key":"10.1016\/j.toxicon.2017.10.005_bib23","doi-asserted-by":"crossref","first-page":"313","DOI":"10.4269\/ajtmh.2000.63.313","article-title":"Inhibition of local hemorrhage and dermonecrosis induced by Bothrops asper snake venom: effectiveness of early in situ administration of the peptidomimetic metalloproteinase inhibitor batimastat and the chelating agent CaNa2EDTA","volume":"63","author":"Rucavado","year":"2000","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.toxicon.2017.10.005_bib24","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/09629350220131980","article-title":"Increments in cytokines and matrix metalloproteinases in skeletal muscle after injection of tissue-damaging toxins from the venom of the snake Bothrops asper","volume":"11","author":"Rucavado","year":"2002","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.toxicon.2017.10.005_bib25","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jprot.2017.02.015","article-title":"Proteomic, toxicological and immunogenic characterization of Mexican west-coast rattlesnake (Crotalus basiliscus) venom and its immunological relatedness with the venom of Central American rattlesnake (Crotalus simus)","volume":"158","author":"Segura \u00c1.","year":"2017","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2017.10.005_bib27","first-page":"949","article-title":"Development of simple standard assay procedures for the characterization of snake venoms","volume":"61","author":"Theakston","year":"1983","journal-title":"Bull. World Health Organ"},{"key":"10.1016\/j.toxicon.2017.10.005_bib28","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/S0041-0101(02)00393-8","article-title":"Report of a WHO workshop on the standardization and control of antivenoms","volume":"41","author":"Theakston","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2017.10.005_bib29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0140-6736(09)61754-2","article-title":"Snake bite","volume":"375","author":"Warrell","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.toxicon.2017.10.005_bib30","series-title":"WHO Guidelines for the Production Control and Regulation of Snake Antivenom Immunoglobulins","author":"World Health Organization (WHO)","year":"2010"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010117303021?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010117303021?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,8,26]],"date-time":"2018-08-26T21:30:17Z","timestamp":1535319017000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010117303021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":29,"alternative-id":["S0041010117303021"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2017.10.005","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An improved technique for the assessment of venom-induced haemorrhage in a murine model","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2017.10.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}