{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T19:52:07Z","timestamp":1778701927271,"version":"3.51.4"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100005298","name":"Universidad de Costa Rica","doi-asserted-by":"publisher","award":["VI-741-B5-602"],"award-info":[{"award-number":["VI-741-B5-602"]}],"id":[{"id":"10.13039\/501100005298","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.toxicon.2018.04.026","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T02:39:39Z","timestamp":1524796779000},"page":"143-148","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina"],"prefix":"10.1016","volume":"148","author":[{"given":"Adolfo","family":"de Roodt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juli\u00e1n","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Solano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2419-6469","authenticated-orcid":false,"given":"Bruno","family":"Lomonte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2018.04.026_bib1","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/S0041-0101(00)00227-0","article-title":"Immunochemical properties of the N-terminal helix of myotoxin II, a Lysine-49 phospholipase A2 from Bothrops asper snake venom","volume":"39","author":"Angulo","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib2","doi-asserted-by":"crossref","first-page":"15514","DOI":"10.1038\/s41598-017-15614-z","article-title":"PLA2-like proteins myotoxic mechanism: a dynamic model description","volume":"7","author":"Borges","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.toxicon.2018.04.026_bib3","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1002\/jms.1242","article-title":"Snake venomics. Strategy and applications","volume":"11","author":"Calvete","year":"2007","journal-title":"J.\u00a0Mass Spectrom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib4","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1016\/S0041-0101(99)00087-2","article-title":"Identification of bothrojaracin-like proteins in snake venoms from Bothrops species and Lachesis muta","volume":"37","author":"Castro","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jprot.2013.06.012","article-title":"Functional proteomic approach to discover geographic variations of king cobra venoms from Southeast Asia and China","volume":"89","author":"Chang","year":"2013","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib6","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/BF01024915","article-title":"Bothropstoxin-I: amino acid sequence and function","volume":"12","author":"Cintra","year":"1993","journal-title":"J.\u00a0Protein Chem."},{"key":"10.1016\/j.toxicon.2018.04.026_bib7","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.toxicon.2011.01.012","article-title":"Toxicity of Bothrops neuwiedi complex (\u201cyarar\u00e1 chica\u201d) venom from different regions of Argentina (Serpentes, Viperidae)","volume":"57","author":"Costa de Oliveira","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib8","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.toxicon.2011.04.013","article-title":"Neutralization of Bothrops alternatus regional venom pools and individual venoms by antivenom: a systematic comparison","volume":"57","author":"de Roodt","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib9","doi-asserted-by":"crossref","first-page":"889","DOI":"10.15517\/rbt.v54i3.13686","article-title":"Caracter\u00edsticas biol\u00f3gicas e inmunol\u00f3gicas del veneno de Bothrops cotiara","volume":"54","author":"de Roodt","year":"2006","journal-title":"Rev. Biol. Trop."},{"key":"10.1016\/j.toxicon.2018.04.026_bib10","doi-asserted-by":"crossref","first-page":"3370","DOI":"10.1021\/acs.jproteome.7b00414","article-title":"Integrated venomics and venom gland transcriptome analysis of juvenile and adult Mexican rattlesnakes Crotalus simus, C. tzabcan, and C.\u00a0culminatus revealed miRNA-modulated ontogenetic shifts","volume":"16","author":"Durban","year":"2017","journal-title":"J.\u00a0Proteom. Res."},{"key":"10.1016\/j.toxicon.2018.04.026_bib11","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1016\/j.bbapap.2014.09.015","article-title":"A\u00a0structure-based proposal for a comprehensive myotoxic mechanism of phospholipase A2-like proteins from viperid snake venoms","volume":"1844","author":"Fernandes","year":"2014","journal-title":"Biochim. Biophys. Acta."},{"key":"10.1016\/j.toxicon.2018.04.026_bib12","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/0041-0101(92)90032-Z","article-title":"Toxic activities of venoms from nine Bothrops species and their correlation with lethality and necrosis","volume":"30","author":"Ferreira","year":"1992","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib13","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/0041-0101(92)90031-Y","article-title":"Neutralization of different activities of venoms from nine species of Bothrops snakes by Bothrops jararaca antivenom","volume":"30","author":"Ferreira","year":"1992","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib14","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/0003-9861(91)90307-5","article-title":"Myotoxin II from Bothrops asper (Terciopelo) venom is a Lysine-49 phospholipase A2","volume":"284","author":"Francis","year":"1991","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2018.04.026_bib15","first-page":"21","article-title":"Bothrops sp. snake venoms: comparison of some biochemical and physicochemical properties and interference in platelet functions","volume":"119","author":"Francischetti","year":"1998","journal-title":"Comp. Biochem. Physiol. C"},{"key":"10.1016\/j.toxicon.2018.04.026_bib16","first-page":"338","article-title":"Ofidios ponzo\u00f1osos de la Argentina","volume":"24","author":"Freiberg","year":"1968","journal-title":"Cienc. T\u00e9cnica"},{"key":"10.1016\/j.toxicon.2018.04.026_bib17","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0041-0101(91)90106-2","article-title":"Comparative study of nine Bothrops snake venoms from adult female snakes and their offspring","volume":"29","author":"Furtado","year":"1991","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib18","first-page":"303","article-title":"Categorizaci\u00f3n del estado de conservaci\u00f3n de las Serpientes de la Rep\u00fablica Argentina","volume":"26","author":"Giraudo","year":"2012","journal-title":"Cuad. Herpetol."},{"key":"10.1016\/j.toxicon.2018.04.026_bib19","series-title":"Serpientes de la selva Paranaense y del Chaco H\u00famedo","author":"Giraudo","year":"2001"},{"key":"10.1016\/j.toxicon.2018.04.026_bib20","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/0041-0101(95)00085-Z","article-title":"Phospholipase A2 myotoxins from Bothrops snake venoms","volume":"33","author":"Guti\u00e9rrez","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib21","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/0041-0101(96)00057-8","article-title":"An aqueous endpoint assay of snake venom phospholipase A2","volume":"34","author":"Holzer","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib22","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.toxicon.2010.01.015","article-title":"A\u00a0study on the venom of Bothrops alternatus Dum\u00e9ril, (Bibron and Dum\u00e9ril) from different regions of Argentina","volume":"55","author":"Lanari","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib23","doi-asserted-by":"crossref","first-page":"860","DOI":"10.2174\/092986609788923356","article-title":"The phospholipase A2 homologues of snake venoms: biological activities and their possible adaptive roles","volume":"16","author":"Lomonte","year":"2009","journal-title":"Prot. Pept. Lett."},{"key":"10.1016\/j.toxicon.2018.04.026_bib24","first-page":"23","article-title":"Strategies in 'snake venomics' aiming at an integrative view of compositional, functional, and immunological characteristics of venoms","volume":"28","author":"Lomonte","year":"2017","journal-title":"J.\u00a0Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2018.04.026_bib25","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.toxicon.2012.02.007","article-title":"Snake venom Lys49 myotoxins: from phospholipases A2 to non-enzymatic membrane disruptors","volume":"60","author":"Lomonte","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib26","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.toxicon.2013.01.006","article-title":"Individual venom profiling of Crotalus durissus terrificus specimens from a geographically limited region: crotamine assessment and captivity evaluation on the biological activities","volume":"69","author":"Louren\u00e7o","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib27","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/0041-0101(95)00012-B","article-title":"Fractionation of Bothrops pirajai snake venom: isolation and characterization of piratoxin-I, a new myotoxic protein","volume":"33","author":"Mancuso","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib28","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1093\/molbev\/msx231","article-title":"Quantity, not quality: rapid adaptation in a polygenic trait proceeded exclusively through expression differentiation","volume":"34","author":"Margres","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.toxicon.2018.04.026_bib29","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1016\/j.jprot.2012.02.035","article-title":"Venom variability and envenoming severity outcomes of the Crotalus scutulatus scutulatus (Mojave rattlesnake) from Southern Arizona","volume":"75","author":"Massey","year":"2012","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib30","series-title":"Gu\u00eda de prevenci\u00f3n, diagn\u00f3stico, tratamiento y vigilancia epidemiol\u00f3gica de los envenenamientos of\u00eddicos","isbn-type":"print","author":"Ministerio de Salud","year":"2014","ISBN":"https:\/\/id.crossref.org\/isbn\/9789503801581"},{"key":"10.1016\/j.toxicon.2018.04.026_bib31","doi-asserted-by":"crossref","first-page":"e569","DOI":"10.7717\/peerj.569","article-title":"Role of enzymatic activity in muscle damage and cytotoxicity induced by Bothrops asper Asp49 phospholipase A2 myotoxins: are there additional effector mechanisms involved?","volume":"2","author":"Mora-Obando","year":"2014","journal-title":"Peer J."},{"key":"10.1016\/j.toxicon.2018.04.026_bib32","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1016\/0041-0101(91)90003-A","article-title":"Neutralization of myotoxic activity of Bothrops venoms by antisera to purified myotoxins and to crude venoms","volume":"29","author":"Moura-da-Silva","year":"1991","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib33","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/0041-0101(90)90094-N","article-title":"Differences in distribution of myotoxic proteins in venoms from different Bothrops species","volume":"28","author":"Moura-da-Silva","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib34","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/0041-0101(91)90063-W","article-title":"Isolation and comparison of myotoxins isolated from venoms of different species of Bothrops snakes","volume":"29","author":"Moura-da-Silva","year":"1991","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib35","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1055\/s-0038-1646781","article-title":"Thrombin-like and factor X-activator components of Bothrops snake venoms","volume":"41","author":"Nahas","year":"1979","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.toxicon.2018.04.026_bib36","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF02933424","article-title":"Screening of Bothrops snake venoms for L-amino acid oxidase activity","volume":"51","author":"Pessatti","year":"1995","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.toxicon.2018.04.026_bib37","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/j.toxicon.2008.10.002","article-title":"Interspecific variation in venom composition and toxicity of Brazilian snakes from Bothrops genus","volume":"52","author":"Queiroz","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib38","doi-asserted-by":"crossref","first-page":"11627","DOI":"10.1016\/S0021-9258(17)39077-4","article-title":"A\u00a0comparison of the crystal structures of phospholipase A2 from bovine pancreas and Crotalus atrox venom","volume":"260","author":"Renetseder","year":"1985","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.toxicon.2018.04.026_bib39","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.toxicon.2012.05.023","article-title":"Mipartoxin-I, a novel three-finger toxin, is the major neurotoxic component in the venom of the redtail coral snake Micrurus mipartitus (Elapidae)","volume":"60","author":"Rey-Su\u00e1rez","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib40","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.toxicon.2011.10.015","article-title":"Venom lethality and diet: differential responses of natural prey and model organisms to the venom of the saw-scaled vipers (Echis)","volume":"59","author":"Richards","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib41","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1006\/abbi.2000.1790","article-title":"Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A2 homologue from Bothrops neuwiedi pauloensis venom","volume":"378","author":"Soares","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.toxicon.2018.04.026_bib42","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jprot.2017.03.003","article-title":"Functional proteomic analyses of Bothrops atrox venom reveals phenotypes associated with habitat variation in the Amazon","volume":"159","author":"Sousa","year":"2017","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib43","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jprot.2015.02.012","article-title":"Venomics, lethality and neutralization of Naja kaouthia (monocled cobra) venoms from three different geographical regions of Southeast Asia","volume":"120","author":"Tan","year":"2015","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib44","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.jprot.2008.07.007","article-title":"Snake venomics of the Brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy markers","volume":"71","author":"Tashima","year":"2008","journal-title":"J.\u00a0Proteom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib45","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1074\/mcp.M112.019331","article-title":"Peptidomics of three Bothrops snake venoms: insights into the molecular diversification of proteomes and peptidomes","volume":"11","author":"Tashima","year":"2012","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.toxicon.2018.04.026_bib46","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0300-9084(00)00202-9","article-title":"Amino acid sequence of piratoxin-II, a myotoxic Lys49 phospholipase A2 homologue from Bothrops pirajai venom","volume":"82","author":"Toyama","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2018.04.026_bib47","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.toxicon.2013.07.008","article-title":"Amino acid sequence and biological characterization of BlatPLA2, a non-toxic acidic phospholipase A2 from the venom of the arboreal snake Bothriechis lateralis from Costa Rica","volume":"73","author":"Van der Laat","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2018.04.026_bib48","series-title":"El porqu\u00e9, cu\u00e1ndo, c\u00f3mo y d\u00f3nde de los ofidios","author":"Vogt","year":"1985"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010118301697?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010118301697?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T13:46:47Z","timestamp":1617716807000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010118301697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":48,"alternative-id":["S0041010118301697"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2018.04.026","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2018.04.026","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}